SLX4_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: SLX4_MOUSE
• UniProt AC: Q6P1D7
• Protein Name: Structure-specific endonuclease subunit SLX4
• Gene Name: Slx4
• Organism: Mus musculus (Mouse).
• Sequence Length: 1565
• Subcellular Localization: Nucleus. Localizes to sites of DNA damage..
• Protein Description: Regulatory subunit that interacts with and increases the activity of different structure-specific endonucleases. Has several distinct roles in protecting genome stability by resolving diverse forms of deleterious DNA structures originating from replication and recombination intermediates and from DNA damage. Component of the SLX1-SLX4 structure-specific endonuclease that resolves DNA secondary structures generated during DNA repair and recombination. Has endonuclease activity towards branched DNA substrates, introducing single-strand cuts in duplex DNA close to junctions with ss-DNA. Has a preference for 5'-flap structures, and promotes symmetrical cleavage of static and migrating Holliday junctions (HJs). Resolves HJs by generating two pairs of ligatable, nicked duplex products. Interacts with the structure-specific ERCC4-ERCC1 endonuclease and promotes the cleavage of bubble structures. Interacts with the structure-specific MUS81-EME1 endonuclease and promotes the cleavage of 3'-flap and replication fork-like structures. SLX4 is required for recovery from alkylation-induced DNA damage and is involved in the resolution of DNA double-strand breaks (By similarity)..
• Protein Sequence: MVPESAPNGNSQPLPSCFTTTGVPSPSKPRVSELVLQRMKQFKRADPERLRHASEESPQKTALGDDVPRSPPEETVGENEYKLDATDSDAAMALALQQEFRREEASSHHDSLEEKGLFFCQMCQKNLSAMNVTRREQHVNRCLDEAEKAQRPASPRIPDCPICGKPFLTTKSRISHLKQCAVRMEVGPQLLLQAVRLQTAQPEVDGSPQVPSFSNNVGGLKRKGVTTKREPRRRKVNKPEAPSEDLLVAMALSRSEVEHCPVVPPLRLENAFSEKIRLGAEKKSRKKRPPVCPPQLVTQDSETTGRQIEDRVAQLLSEEAELSCTPPLLASKISKEELEPAGWRARLPEGKRNFLWELSALTGAWAEESFYTVGLFPPIVSQCPSKEPQLPLELPKQGEPSPRRPPASQSSLPVSHSPKIRLLSSSQRERQALQDLVDLAVEGLSSSPQPGSRGVPTGLDLVPSSLPLTGFVLPCKKTLKKDDSASLSLGLLVTDFGAMVNNPHLSDVQFQLDSGEVLYAHKFVLYARCPLLIQYVSTESFSSEEDGDLTQRALLSDVSSEAAHAFLNYLYMADTDMPPSLVPDLRSLALRFGVSDLVQLCEQVPAVVDLEGEQPEETSEDCESRAETFLELLRSVWVDNEEEVETLLKPELCEEERERVNEAEMEEIYEFAATQRKLLQWGRAADPDGSTNPHGEDGAVSEPSLAGVQSNRQLENTEHMESSGLEKEEALASWEQEGHSTPLQDQCPDWAGKAEAQDALGEATDDPSFCSRHRRGKECLPLHPNKAHGCKQPLPSNPRVSSELSQITVDHEEQSDHVRETQADMAQAPTPHSCSLVSQSSVDGSPSQSWLHLYHTSHLSPSVSQSHSSISRVASPRSLSPTTPTKQRRGSNIVTLRKDAGHHRGQQSSPIAGHRNRGILISPAKSPPIDLTQSVPEPLSPRAQDPLHFVKKEDEVILLLDSDEELEHTKTESVSKDSPEGRKVPEFSPRSSELFSVIDVEEDHEHFQSPLKREAGLQHGEEGQLGNQSALGCRDIPWLLCSQKTSLDEDSATDTSWLVPATPGVSRSRDCSSQTQIKSLKTRIPSDETAQQTPRPNLERRTMLETAQQFSVIMPHTQPITLGAFDSGRQAYRSPSHPYPRHHRLSSSQPSCPGPDFTRWSQKSSAPRPCLPNLPAADDVVEVGDSDDEVASHQGNSSPVLDGDPPGPMGDYCWNEPLSPIPIDHLNLERTGPLTTSSPSSQVLEALHSDDCHSPGLGTTPIRGSCGTLRESQERSSLAGSPEALWDDWNEEEGQSPEAPPVAQMLSTRTRKPDRPETPKGANQKKNLPPKVPITPMPRYSIMETPVLKKELDRFGVRALPKRQMVLKLKEIFQYTHQTLESDSEDEVQSPQIPAELPCRQASTTETCNPSRLPTGEPSHPDGDAQLPASQESMATSVDGSDNSFSSKSSSAEFGAAFEYSDEDKDEEVGVTASQAAIQAADTEEAVRRYIRSKPALHRQVLRYQPVELAELQAELKQNGIPVAMGKLSDILDAQCITFTTAAARKEKLKHKRRQPSGRKKKDQK

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
20	Phosphorylation	PLPSCFTTTGVPSPS CCCCCEECCCCCCCC	10.99	22817900
21	Phosphorylation	LPSCFTTTGVPSPSK CCCCEECCCCCCCCC	32.88	21183079
25	Phosphorylation	FTTTGVPSPSKPRVS EECCCCCCCCCCCHH	39.12	21183079
27	Phosphorylation	TTGVPSPSKPRVSEL CCCCCCCCCCCHHHH	60.68	21183079
54	Phosphorylation	PERLRHASEESPQKT HHHHHHCCCCCCCCC	35.45	25266776
57	Phosphorylation	LRHASEESPQKTALG HHHCCCCCCCCCCCC	28.69	25266776
61	Phosphorylation	SEESPQKTALGDDVP CCCCCCCCCCCCCCC	23.13	25293948
70	Phosphorylation	LGDDVPRSPPEETVG CCCCCCCCCCHHCCC	38.44	25619855
75	Phosphorylation	PRSPPEETVGENEYK CCCCCHHCCCCCCEE	31.95	25619855
81	Phosphorylation	ETVGENEYKLDATDS HCCCCCCEECCCCCH	28.56	25293948
86	Phosphorylation	NEYKLDATDSDAAMA CCEECCCCCHHHHHH	35.38	25293948
88	Phosphorylation	YKLDATDSDAAMALA EECCCCCHHHHHHHH	25.46	25293948
111	Phosphorylation	EASSHHDSLEEKGLF HHHHCCHHHHHHCHH	33.07	-
154	Phosphorylation	EKAQRPASPRIPDCP HHHCCCCCCCCCCCC	19.86	30635358
165	Ubiquitination	PDCPICGKPFLTTKS CCCCCCCCEECCCHH	28.34	-
323	Phosphorylation	LSEEAELSCTPPLLA HCHHHHHCCCHHHHH	13.88	25293948
325	Phosphorylation	EEAELSCTPPLLASK HHHHHCCCHHHHHHH	25.27	25266776
401	Phosphorylation	LPKQGEPSPRRPPAS CCCCCCCCCCCCCCC	27.08	29514104
410	Phosphorylation	RRPPASQSSLPVSHS CCCCCCCCCCCCCCC	31.20	24719451
415	Phosphorylation	SQSSLPVSHSPKIRL CCCCCCCCCCCCEEE	19.13	25619855
417	Phosphorylation	SSLPVSHSPKIRLLS CCCCCCCCCCEEECC	22.00	25619855
424	Phosphorylation	SPKIRLLSSSQRERQ CCCEEECCCCHHHHH	32.32	30482847
425	Phosphorylation	PKIRLLSSSQRERQA CCEEECCCCHHHHHH	30.38	28066266
426	Phosphorylation	KIRLLSSSQRERQAL CEEECCCCHHHHHHH	29.85	25266776
445	Phosphorylation	DLAVEGLSSSPQPGS HHHHHHCCCCCCCCC	39.36	23984901
446	Phosphorylation	LAVEGLSSSPQPGSR HHHHHCCCCCCCCCC	51.59	21149613
447	Phosphorylation	AVEGLSSSPQPGSRG HHHHCCCCCCCCCCC	24.95	21149613
452	Phosphorylation	SSSPQPGSRGVPTGL CCCCCCCCCCCCCCC	32.32	23984901
464	Phosphorylation	TGLDLVPSSLPLTGF CCCCCCCCCCCCCCE	36.71	22006019
635	Phosphorylation	TFLELLRSVWVDNEE HHHHHHHHHCCCCHH	21.94	30635358
805	Phosphorylation	PRVSSELSQITVDHE CCCCHHHHCEECCHH	18.58	-
845	Phosphorylation	SQSSVDGSPSQSWLH ECCCCCCCCCCCCHH	19.53	-
875	Phosphorylation	SSISRVASPRSLSPT CCCCCCCCCCCCCCC	20.28	22817900
878	Phosphorylation	SRVASPRSLSPTTPT CCCCCCCCCCCCCCC	36.26	28833060
880	Phosphorylation	VASPRSLSPTTPTKQ CCCCCCCCCCCCCCC	22.80	26824392
882	Phosphorylation	SPRSLSPTTPTKQRR CCCCCCCCCCCCCCC	41.87	28833060
883	Phosphorylation	PRSLSPTTPTKQRRG CCCCCCCCCCCCCCC	32.14	28833060
885	Phosphorylation	SLSPTTPTKQRRGSN CCCCCCCCCCCCCCC	37.40	28833060
891	Phosphorylation	PTKQRRGSNIVTLRK CCCCCCCCCCEEEEC	22.90	27600695
908	Phosphorylation	GHHRGQQSSPIAGHR CCCCCCCCCCCCCCC	30.14	25266776
909	Phosphorylation	HHRGQQSSPIAGHRN CCCCCCCCCCCCCCC	18.91	26824392
922	Phosphorylation	RNRGILISPAKSPPI CCCCEEEECCCCCCC	18.50	28973931
926	Phosphorylation	ILISPAKSPPIDLTQ EEEECCCCCCCCCCC	37.71	26824392
932	Phosphorylation	KSPPIDLTQSVPEPL CCCCCCCCCCCCCCC	18.30	21082442
934	Phosphorylation	PPIDLTQSVPEPLSP CCCCCCCCCCCCCCC	34.11	25619855
940	Phosphorylation	QSVPEPLSPRAQDPL CCCCCCCCCCCCCCC	24.05	26824392
962	Phosphorylation	EVILLLDSDEELEHT EEEEEECCHHHHHCC	47.06	25619855
975	Phosphorylation	HTKTESVSKDSPEGR CCCCCCCCCCCCCCC	39.77	25159016
978	Phosphorylation	TESVSKDSPEGRKVP CCCCCCCCCCCCCCC	28.70	25159016
988	Phosphorylation	GRKVPEFSPRSSELF CCCCCCCCCCCHHCE	20.10	26824392
1009	Phosphorylation	EDHEHFQSPLKREAG CCHHHHCCHHHHHHC	31.12	25266776
1029	Phosphorylation	EGQLGNQSALGCRDI CCCCCCCCCCCCCCC	29.48	28066266
1045	Phosphorylation	WLLCSQKTSLDEDSA CEEECCCCCCCCCCC	27.21	30635358
1046	Phosphorylation	LLCSQKTSLDEDSAT EEECCCCCCCCCCCC	40.64	30635358
1051	Phosphorylation	KTSLDEDSATDTSWL CCCCCCCCCCCCCCE	30.90	30635358
1053	Phosphorylation	SLDEDSATDTSWLVP CCCCCCCCCCCCEEE	43.70	30635358
1055	Phosphorylation	DEDSATDTSWLVPAT CCCCCCCCCCEEECC	19.90	30635358
1056	Phosphorylation	EDSATDTSWLVPATP CCCCCCCCCEEECCC	22.63	30635358
1086	Phosphorylation	SLKTRIPSDETAQQT HHHHCCCCHHHHHCC	45.79	25338131
1117	Phosphorylation	FSVIMPHTQPITLGA CCEECCCCCCCEECE	30.12	17525332
1132	Phosphorylation	FDSGRQAYRSPSHPY ECCCCCCCCCCCCCC	11.86	26643407
1134	Phosphorylation	SGRQAYRSPSHPYPR CCCCCCCCCCCCCCC	20.34	26643407
1136	Phosphorylation	RQAYRSPSHPYPRHH CCCCCCCCCCCCCCC	38.04	28066266
1146	Phosphorylation	YPRHHRLSSSQPSCP CCCCCCCCCCCCCCC	28.09	28066266
1147	Phosphorylation	PRHHRLSSSQPSCPG CCCCCCCCCCCCCCC	37.27	28066266
1148	Phosphorylation	RHHRLSSSQPSCPGP CCCCCCCCCCCCCCC	42.61	28066266
1231	Phosphorylation	DHLNLERTGPLTTSS CCCCCCCCCCCCCCC	33.50	25293948
1235	Phosphorylation	LERTGPLTTSSPSSQ CCCCCCCCCCCCHHH	27.97	25293948
1236	Phosphorylation	ERTGPLTTSSPSSQV CCCCCCCCCCCHHHH	34.58	25293948
1237	Phosphorylation	RTGPLTTSSPSSQVL CCCCCCCCCCHHHHH	34.71	25293948
1238	Phosphorylation	TGPLTTSSPSSQVLE CCCCCCCCCHHHHHH	27.06	19060867
1240	Phosphorylation	PLTTSSPSSQVLEAL CCCCCCCHHHHHHHH	35.04	22817900
1241	Phosphorylation	LTTSSPSSQVLEALH CCCCCCHHHHHHHHH	27.91	25293948
1249	Phosphorylation	QVLEALHSDDCHSPG HHHHHHHCCCCCCCC	35.67	22817900
1254	Phosphorylation	LHSDDCHSPGLGTTP HHCCCCCCCCCCCCC	27.30	22817900
1259	Phosphorylation	CHSPGLGTTPIRGSC CCCCCCCCCCCCCCC	34.15	22817900
1260	Phosphorylation	HSPGLGTTPIRGSCG CCCCCCCCCCCCCCC	18.21	21183079
1276	Phosphorylation	LRESQERSSLAGSPE HHHHHHHHHHCCCHH	29.02	25293948
1277	Phosphorylation	RESQERSSLAGSPEA HHHHHHHHHCCCHHH	28.58	25293948
1281	Phosphorylation	ERSSLAGSPEALWDD HHHHHCCCHHHHCCC	17.60	25293948
1296	Phosphorylation	WNEEEGQSPEAPPVA CCCCCCCCCCCCCHH	35.25	25293948
1307	Phosphorylation	PPVAQMLSTRTRKPD CCHHHHHHCCCCCCC	15.53	25293948
1308	Phosphorylation	PVAQMLSTRTRKPDR CHHHHHHCCCCCCCC	31.90	25293948
1325	Acetylation	TPKGANQKKNLPPKV CCCCCCCCCCCCCCC	43.36	7844401
1326	Acetylation	PKGANQKKNLPPKVP CCCCCCCCCCCCCCC	54.69	7844413
1340	Phosphorylation	PITPMPRYSIMETPV CCCCCCCCCCCCCHH	9.18	28833060
1341	Phosphorylation	ITPMPRYSIMETPVL CCCCCCCCCCCCHHH	19.42	28833060
1345	Phosphorylation	PRYSIMETPVLKKEL CCCCCCCCHHHHHHH	11.00	26370283
1375	Phosphorylation	KLKEIFQYTHQTLES HHHHHHHHHHHHCCC	8.74	25293948
1376	Phosphorylation	LKEIFQYTHQTLESD HHHHHHHHHHHCCCC	9.20	25293948
1379	Phosphorylation	IFQYTHQTLESDSED HHHHHHHHCCCCCCC	25.53	25293948
1382	Phosphorylation	YTHQTLESDSEDEVQ HHHHHCCCCCCCCCC	49.93	22817900
1384	Phosphorylation	HQTLESDSEDEVQSP HHHCCCCCCCCCCCC	57.11	22817900
1390	Phosphorylation	DSEDEVQSPQIPAEL CCCCCCCCCCCCCCC	24.77	25293948
1403	Phosphorylation	ELPCRQASTTETCNP CCCCCCCCCCCCCCH	27.76	25521595

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of SLX4_MOUSE !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of SLX4_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of SLX4_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
MUS81_HUMAN	MUS81	physical	25224045
EME1_HUMAN	EME1	physical	25224045
XPF_HUMAN	ERCC4	physical	25722289
MUS81_HUMAN	MUS81	physical	25722289
PARP1_HUMAN	PARP1	physical	25722289
ERCC1_MOUSE	Ercc1	physical	25538220
UBP45_MOUSE	Usp45	physical	25538220
XPF_MOUSE	Ercc4	physical	24726326
ERCC1_MOUSE	Ercc1	physical	24726326
ERCC1_MOUSE	Ercc1	physical	21240276
MUS81_MOUSE	Mus81	physical	21240276
SLX1_MOUSE	Slx1b	physical	21240276

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"ATM and ATR substrate analysis reveals extensive protein networksresponsive to DNA damage.";
Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III,Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N.,Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J.;
Science 316:1160-1166(2007).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-1117, AND MASSSPECTROMETRY.
PubMed: 17525332