dbPTM

RPN1_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	RPN1_HUMAN
UniProt AC	P04843
Protein Name	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1
Gene Name	RPN1
Organism	Homo sapiens (Human).
Sequence Length	607
Subcellular Localization	Endoplasmic reticulum . Endoplasmic reticulum membrane Single-pass type I membrane protein . Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Protein Description	Essential subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains..
Protein Sequence	MEAPAAGLFLLLLLGTWAPAPGSASSEAPPLINEDVKRTVDLSSHLAKVTAEVVLAHLGGGSTSRATSFLLALEPELEARLAHLGVQVKGEDEEENNLEVRETKIKGKSGRFFTVKLPVALDPGAKISVIVETVYTHVLHPYPTQITQSEKQFVVFEGNHYFYSPYPTKTQTMRVKLASRNVESYTKLGNPTRSEDLLDYGPFRDVPAYSQDTFKVHYENNSPFLTITSMTRVIEVSHWGNIAVEENVDLKHTGAVLKGPFSRYDYQRQPDSGISSIRSFKTILPAAAQDVYYRDEIGNVSTSHLLILDDSVEMEIRPRFPLFGGWKTHYIVGYNLPSYEYLYNLGDQYALKMRFVDHVFDEQVIDSLTVKIILPEGAKNIEIDSPYEISRAPDELHYTYLDTFGRPVIVAYKKNLVEQHIQDIVVHYTFNKVLMLQEPLLVVAAFYILFFTVIIYVRLDFSITKDPAAEARMKVACITEQVLTLVNKRIGLYRHFDETVNRYKQSRDISTLNSGKKSLETEHKALTSEIALLQSRLKTEGSDLCDRVSEMQKLDAQVKELVLKSAVEAERLVAGKLKKDTYIENEKLISGKRQELVTKIDHILDAL

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
37	Ubiquitination	PLINEDVKRTVDLSS CCCCCCHHHHHHHHH	55.46	-
39	Phosphorylation	INEDVKRTVDLSSHL CCCCHHHHHHHHHHH	16.80	21406692
43	Phosphorylation	VKRTVDLSSHLAKVT HHHHHHHHHHHHHHH	15.96	21406692
44	Phosphorylation	KRTVDLSSHLAKVTA HHHHHHHHHHHHHHH	29.80	21406692
48	Ubiquitination	DLSSHLAKVTAEVVL HHHHHHHHHHHHHHH	46.62	-
50	O-linked_Glycosylation	SSHLAKVTAEVVLAH HHHHHHHHHHHHHHH	19.18	OGP
62	Phosphorylation	LAHLGGGSTSRATSF HHHCCCCCHHHHHHH	26.87	24719451
63	Phosphorylation	AHLGGGSTSRATSFL HHCCCCCHHHHHHHH	26.05	28348404
64	Phosphorylation	HLGGGSTSRATSFLL HCCCCCHHHHHHHHH	22.93	26437602
67	Phosphorylation	GGSTSRATSFLLALE CCCHHHHHHHHHHHC	20.92	30087585
68	Phosphorylation	GSTSRATSFLLALEP CCHHHHHHHHHHHCH	16.95	30087585
89	Ubiquitination	AHLGVQVKGEDEEEN HHCCCEECCCCCCCC	39.22	16196087
108	Ubiquitination	RETKIKGKSGRFFTV EEEEEECCCCCEEEE	44.74	24816145
116	Ubiquitination	SGRFFTVKLPVALDP CCCEEEEEEEEEECC	43.97	23000965
135	Phosphorylation	SVIVETVYTHVLHPY EEEEEEHHHCCCCCC	9.88	-
136	Phosphorylation	VIVETVYTHVLHPYP EEEEEHHHCCCCCCC	10.94	-
144	O-linked_Glycosylation	HVLHPYPTQITQSEK CCCCCCCCCCCCCCC	27.10	OGP
151	Ubiquitination	TQITQSEKQFVVFEG CCCCCCCCEEEEEEC	55.04	16196087
155	Ubiquitination	QSEKQFVVFEGNHYF CCCCEEEEEECCEEE	3.69	21890473
161	Phosphorylation	VVFEGNHYFYSPYPT EEEECCEEEECCCCC	14.28	28152594
163	Phosphorylation	FEGNHYFYSPYPTKT EECCEEEECCCCCCC	11.11	28152594
164	Phosphorylation	EGNHYFYSPYPTKTQ ECCEEEECCCCCCCC	14.20	28152594
166	Phosphorylation	NHYFYSPYPTKTQTM CEEEECCCCCCCCEE	20.00	28152594
168	Phosphorylation	YFYSPYPTKTQTMRV EEECCCCCCCCEEEE	40.66	28152594
176	Ubiquitination	KTQTMRVKLASRNVE CCCEEEEEEHHCCHH	28.15	27667366
184	Phosphorylation	LASRNVESYTKLGNP EHHCCHHHHHCCCCC	33.54	28152594
185	Phosphorylation	ASRNVESYTKLGNPT HHCCHHHHHCCCCCC	8.39	26437602
186	Phosphorylation	SRNVESYTKLGNPTR HCCHHHHHCCCCCCC	29.13	28152594
187	2-Hydroxyisobutyrylation	RNVESYTKLGNPTRS CCHHHHHCCCCCCCC	46.28	-
187	Acetylation	RNVESYTKLGNPTRS CCHHHHHCCCCCCCC	46.28	19608861
187	Malonylation	RNVESYTKLGNPTRS CCHHHHHCCCCCCCC	46.28	26320211
187	Ubiquitination	RNVESYTKLGNPTRS CCHHHHHCCCCCCCC	46.28	27667366
194	Phosphorylation	KLGNPTRSEDLLDYG CCCCCCCCHHHHHCC	37.63	21712546
200	Phosphorylation	RSEDLLDYGPFRDVP CCHHHHHCCCCCCCC	27.03	28152594
209	Phosphorylation	PFRDVPAYSQDTFKV CCCCCCCCCCCCEEE	10.78	28152594
210	Phosphorylation	FRDVPAYSQDTFKVH CCCCCCCCCCCEEEE	24.56	28152594
213	Phosphorylation	VPAYSQDTFKVHYEN CCCCCCCCEEEEECC	20.09	28152594
215	Ubiquitination	AYSQDTFKVHYENNS CCCCCCEEEEECCCC	30.14	21906983
222	Phosphorylation	KVHYENNSPFLTITS EEEECCCCCCEEEEE	28.72	-
226	Ubiquitination	ENNSPFLTITSMTRV CCCCCCEEEEEEEEE	23.69	21890473
228	O-linked_Glycosylation	NSPFLTITSMTRVIE CCCCEEEEEEEEEEE	13.75	OGP
231	O-linked_Glycosylation	FLTITSMTRVIEVSH CEEEEEEEEEEEEEC	22.88	OGP
254	Ubiquitination	NVDLKHTGAVLKGPF CCCCCCCCCEEECCC	17.26	21890473
258	Ubiquitination	KHTGAVLKGPFSRYD CCCCCEEECCCCCCC	58.92	23000965
264	Phosphorylation	LKGPFSRYDYQRQPD EECCCCCCCCCCCCC	20.15	-
266	Phosphorylation	GPFSRYDYQRQPDSG CCCCCCCCCCCCCCC	9.17	-
272	Phosphorylation	DYQRQPDSGISSIRS CCCCCCCCCCCHHHH	44.71	21406692
275	Phosphorylation	RQPDSGISSIRSFKT CCCCCCCCHHHHHCH	24.20	24719451
276	Phosphorylation	QPDSGISSIRSFKTI CCCCCCCHHHHHCHH	21.79	25954137
281	2-Hydroxyisobutyrylation	ISSIRSFKTILPAAA CCHHHHHCHHHHHHH	35.58	-
281	Ubiquitination	ISSIRSFKTILPAAA CCHHHHHCHHHHHHH	35.58	21890473
292	Phosphorylation	PAAAQDVYYRDEIGN HHHHCCCEECCCCCC	10.89	28152594
293	Phosphorylation	AAAQDVYYRDEIGNV HHHCCCEECCCCCCC	16.07	28152594
297	Ubiquitination	DVYYRDEIGNVSTSH CCEECCCCCCCCCCE	5.95	21890473
299	N-linked_Glycosylation	YYRDEIGNVSTSHLL EECCCCCCCCCCEEE	30.17	19159218
320	Ubiquitination	EMEIRPRFPLFGGWK EEEECCCCCCCCCCE	7.36	21890473
352	Ubiquitination	LGDQYALKMRFVDHV CCCCHHHHHHHCCCC	21.62	21906983
367	O-linked_Glycosylation	FDEQVIDSLTVKIIL CCHHHHHEEEEEEEC	18.15	23301498
371	Ubiquitination	VIDSLTVKIILPEGA HHHEEEEEEECCCCC	20.85	21906983
379	2-Hydroxyisobutyrylation	IILPEGAKNIEIDSP EECCCCCCCCEECCC	70.12	-
379	Ubiquitination	IILPEGAKNIEIDSP EECCCCCCCCEECCC	70.12	21906983
385	Phosphorylation	AKNIEIDSPYEISRA CCCCEECCCCCCCCC	34.73	28152594
387	Phosphorylation	NIEIDSPYEISRAPD CCEECCCCCCCCCCC	29.82	28152594
390	Phosphorylation	IDSPYEISRAPDELH ECCCCCCCCCCCCCE	15.51	28152594
391	Ubiquitination	DSPYEISRAPDELHY CCCCCCCCCCCCCEE	56.66	21890473
398	Phosphorylation	RAPDELHYTYLDTFG CCCCCCEEEEECCCC	15.09	-
399	Phosphorylation	APDELHYTYLDTFGR CCCCCEEEEECCCCC	13.58	-
410	Ubiquitination	TFGRPVIVAYKKNLV CCCCEEEEEECCCHH	4.92	21890473
412	Phosphorylation	GRPVIVAYKKNLVEQ CCEEEEEECCCHHHH	16.17	28152594
413	Acetylation	RPVIVAYKKNLVEQH CEEEEEECCCHHHHH	26.00	26051181
413	Ubiquitination	RPVIVAYKKNLVEQH CEEEEEECCCHHHHH	26.00	21906983
414	Acetylation	PVIVAYKKNLVEQHI EEEEEECCCHHHHHH	42.72	156415
414	Ubiquitination	PVIVAYKKNLVEQHI EEEEEECCCHHHHHH	42.72	-
418	Ubiquitination	AYKKNLVEQHIQDIV EECCCHHHHHHHHHH	39.34	21890473
452	Ubiquitination	AFYILFFTVIIYVRL HHHHHHHHHHHHHHC	11.94	21890473
465	Ubiquitination	RLDFSITKDPAAEAR HCCCCCCCCHHHHHH	61.11	21906983
474	Ubiquitination	PAAEARMKVACITEQ HHHHHHHHHHHHHHH	22.56	-
477	S-nitrosocysteine	EARMKVACITEQVLT HHHHHHHHHHHHHHH	4.41	-
477	Glutathionylation	EARMKVACITEQVLT HHHHHHHHHHHHHHH	4.41	22555962
477	S-nitrosylation	EARMKVACITEQVLT HHHHHHHHHHHHHHH	4.41	20140087
477	S-palmitoylation	EARMKVACITEQVLT HHHHHHHHHHHHHHH	4.41	29575903
488	2-Hydroxyisobutyrylation	QVLTLVNKRIGLYRH HHHHHHHHHHCCHHH	37.60	-
488	Acetylation	QVLTLVNKRIGLYRH HHHHHHHHHHCCHHH	37.60	25953088
488	Ubiquitination	QVLTLVNKRIGLYRH HHHHHHHHHHCCHHH	37.60	21906983
504	Ubiquitination	DETVNRYKQSRDIST HHHHHHHHHHCCHHH	38.26	21890473
510	Phosphorylation	YKQSRDISTLNSGKK HHHHCCHHHHHHCCC	31.00	21815630
511	Phosphorylation	KQSRDISTLNSGKKS HHHCCHHHHHHCCCC	30.23	24719451
514	Phosphorylation	RDISTLNSGKKSLET CCHHHHHHCCCCHHH	55.92	21815630
516	2-Hydroxyisobutyrylation	ISTLNSGKKSLETEH HHHHHHCCCCHHHHH	38.72	-
516	Acetylation	ISTLNSGKKSLETEH HHHHHHCCCCHHHHH	38.72	26051181
516	Malonylation	ISTLNSGKKSLETEH HHHHHHCCCCHHHHH	38.72	26320211
516	Ubiquitination	ISTLNSGKKSLETEH HHHHHHCCCCHHHHH	38.72	27667366
517	Ubiquitination	STLNSGKKSLETEHK HHHHHCCCCHHHHHH	64.89	29967540
518	Phosphorylation	TLNSGKKSLETEHKA HHHHCCCCHHHHHHH	34.62	26657352
521	Phosphorylation	SGKKSLETEHKALTS HCCCCHHHHHHHHHH	49.00	28102081
524	Acetylation	KSLETEHKALTSEIA CCHHHHHHHHHHHHH	38.57	23954790
524	Malonylation	KSLETEHKALTSEIA CCHHHHHHHHHHHHH	38.57	26320211
524	Ubiquitination	KSLETEHKALTSEIA CCHHHHHHHHHHHHH	38.57	29967540
527	Phosphorylation	ETEHKALTSEIALLQ HHHHHHHHHHHHHHH	29.22	25693802
527	Ubiquitination	ETEHKALTSEIALLQ HHHHHHHHHHHHHHH	29.22	21890473
528	Phosphorylation	TEHKALTSEIALLQS HHHHHHHHHHHHHHH	28.05	21712546
535	Phosphorylation	SEIALLQSRLKTEGS HHHHHHHHHHHHCCC	39.28	30266825
538	2-Hydroxyisobutyrylation	ALLQSRLKTEGSDLC HHHHHHHHHCCCHHH	43.34	-
538	Acetylation	ALLQSRLKTEGSDLC HHHHHHHHHCCCHHH	43.34	23749302
538	Malonylation	ALLQSRLKTEGSDLC HHHHHHHHHCCCHHH	43.34	26320211
538	Sumoylation	ALLQSRLKTEGSDLC HHHHHHHHHCCCHHH	43.34	25114211
538	Ubiquitination	ALLQSRLKTEGSDLC HHHHHHHHHCCCHHH	43.34	27667366
542	Phosphorylation	SRLKTEGSDLCDRVS HHHHHCCCHHHHHHH	22.60	23312004
545	Glutathionylation	KTEGSDLCDRVSEMQ HHCCCHHHHHHHHHH	3.64	22555962
547	Methylation	EGSDLCDRVSEMQKL CCCHHHHHHHHHHHH	32.63	115492375
553	Acetylation	DRVSEMQKLDAQVKE HHHHHHHHHHHHHHH	47.20	23954790
553	Malonylation	DRVSEMQKLDAQVKE HHHHHHHHHHHHHHH	47.20	26320211
553	Ubiquitination	DRVSEMQKLDAQVKE HHHHHHHHHHHHHHH	47.20	23000965
555	Ubiquitination	VSEMQKLDAQVKELV HHHHHHHHHHHHHHH	41.83	21890473
559	2-Hydroxyisobutyrylation	QKLDAQVKELVLKSA HHHHHHHHHHHHHHH	33.00	-
559	Acetylation	QKLDAQVKELVLKSA HHHHHHHHHHHHHHH	33.00	26822725
559	Malonylation	QKLDAQVKELVLKSA HHHHHHHHHHHHHHH	33.00	26320211
559	Ubiquitination	QKLDAQVKELVLKSA HHHHHHHHHHHHHHH	33.00	23000965
564	2-Hydroxyisobutyrylation	QVKELVLKSAVEAER HHHHHHHHHHHHHHH	28.96	-
564	Acetylation	QVKELVLKSAVEAER HHHHHHHHHHHHHHH	28.96	25953088
564	Malonylation	QVKELVLKSAVEAER HHHHHHHHHHHHHHH	28.96	26320211
564	Ubiquitination	QVKELVLKSAVEAER HHHHHHHHHHHHHHH	28.96	23000965
576	Acetylation	AERLVAGKLKKDTYI HHHHHCCCCCCCCEE	47.60	25953088
576	Malonylation	AERLVAGKLKKDTYI HHHHHCCCCCCCCEE	47.60	26320211
576	Ubiquitination	AERLVAGKLKKDTYI HHHHHCCCCCCCCEE	47.60	33845483
577	Ubiquitination	ERLVAGKLKKDTYIE HHHHCCCCCCCCEEC	9.02	21890473
578	Acetylation	RLVAGKLKKDTYIEN HHHCCCCCCCCEECC	52.68	23749302
578	Ubiquitination	RLVAGKLKKDTYIEN HHHCCCCCCCCEECC	52.68	21963094
579	Acetylation	LVAGKLKKDTYIENE HHCCCCCCCCEECCC	66.78	26051181
579	Ubiquitination	LVAGKLKKDTYIENE HHCCCCCCCCEECCC	66.78	21963094
581	Phosphorylation	AGKLKKDTYIENEKL CCCCCCCCEECCCCC	34.97	28152594
582	Phosphorylation	GKLKKDTYIENEKLI CCCCCCCEECCCCCC	19.17	28152594
587	2-Hydroxyisobutyrylation	DTYIENEKLISGKRQ CCEECCCCCCCCCHH	63.80	-
587	Acetylation	DTYIENEKLISGKRQ CCEECCCCCCCCCHH	63.80	23954790
587	Malonylation	DTYIENEKLISGKRQ CCEECCCCCCCCCHH	63.80	26320211
587	Ubiquitination	DTYIENEKLISGKRQ CCEECCCCCCCCCHH	63.80	33845483
592	Ubiquitination	NEKLISGKRQELVTK CCCCCCCCHHHHHHH	44.08	21890473
598	Phosphorylation	GKRQELVTKIDHILD CCHHHHHHHHHHHHH	34.60	29496963
598	Ubiquitination	GKRQELVTKIDHILD CCHHHHHHHHHHHHH	34.60	21890473
599	Ubiquitination	KRQELVTKIDHILDA CHHHHHHHHHHHHHH	38.36	29967540

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
-	K	Ubiquitination	E3 ubiquitin ligase	ASB11	Q8WXH4	PMID:24337577

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of RPN1_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of RPN1_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
OS9_HUMAN	OS9	physical	18711132
GRP78_HUMAN	HSPA5	physical	10588643
STT3B_HUMAN	STT3B	physical	22939629
RPN2_HUMAN	RPN2	physical	22939629
SSRG_HUMAN	SSR3	physical	22939629
RSSA_HUMAN	RPSA	physical	22939629
TECR_HUMAN	TECR	physical	22939629
VDAC2_HUMAN	VDAC2	physical	22939629
SND1_HUMAN	SND1	physical	22939629
SGTA_HUMAN	SGTA	physical	25416956
UBQL1_HUMAN	UBQLN1	physical	25416956
ATPG_HUMAN	ATP5C1	physical	26344197
CALX_HUMAN	CANX	physical	26344197
CCD47_HUMAN	CCDC47	physical	26344197
CLGN_HUMAN	CLGN	physical	26344197
CY1_HUMAN	CYC1	physical	26344197
IDH3A_HUMAN	IDH3A	physical	26344197
COX2_HUMAN	COX2	physical	26344197
NDUAC_HUMAN	NDUFA12	physical	26344197
NDUB8_HUMAN	NDUFB8	physical	26344197
PYC_HUMAN	PC	physical	26344197
PPIB_HUMAN	PPIB	physical	26344197
RAB2A_HUMAN	RAB2A	physical	26344197
RL35_HUMAN	RPL35	physical	26344197
SSRA_HUMAN	SSR1	physical	26344197
SSRD_HUMAN	SSR4	physical	26344197
EFTU_HUMAN	TUFM	physical	26344197
UBC_HUMAN	UBC	physical	26912900

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
There are no disease associations of PTM sites.
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of RPN1_HUMAN

Related Literatures of Post-Translational Modification

Acetylation
Reference	PubMed
"Lysine acetylation targets protein complexes and co-regulates majorcellular functions."; Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.,Olsen J.V., Mann M.; Science 325:834-840(2009). Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-187, AND MASS SPECTROMETRY.	19608861 [Abstract]
N-linked Glycosylation
Reference	PubMed
"Glycoproteomics analysis of human liver tissue by combination ofmultiple enzyme digestion and hydrazide chemistry."; Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.; J. Proteome Res. 8:651-661(2009). Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-299, AND MASSSPECTROMETRY.	19159218 [Abstract]