dbPTM

P85A_MOUSE - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	P85A_MOUSE
UniProt AC	P26450
Protein Name	Phosphatidylinositol 3-kinase regulatory subunit alpha
Gene Name	Pik3r1
Organism	Mus musculus (Mouse).
Sequence Length	724
Subcellular Localization
Protein Description	Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling (By similarity). Modulates the cellular response to ER stress by promoting nuclear translocation of XBP1 isoform 2 in a ER stress- and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement. [PubMed: 20348926]
Protein Sequence	MSAEGYQYRALYDYKKEREEDIDLHLGDILTVNKGSLVALGFSDGQEARPEDIGWLNGYNETTGERGDFPGTYVEYIGRKRISPPTPKPRPPRPLPVAPGSSKTEADTEQQALPLPDLAEQFAPPDVAPPLLIKLLEAIEKKGLECSTLYRTQSSSNPAELRQLLDCDAASVDLEMIDVHVLADAFKRYLADLPNPVIPVAVYNEMMSLAQELQSPEDCIQLLKKLIRLPNIPHQCWLTLQYLLKHFFKLSQASSKNLLNARVLSEIFSPVLFRFPAASSDNTEHLIKAIEILISTEWNERQPAPALPPKPPKPTTVANNSMNNNMSLQDAEWYWGDISREEVNEKLRDTADGTFLVRDASTKMHGDYTLTLRKGGNNKLIKIFHRDGKYGFSDPLTFNSVVELINHYRNESLAQYNPKLDVKLLYPVSKYQQDQVVKEDNIEAVGKKLHEYNTQFQEKSREYDRLYEEYTRTSQEIQMKRTAIEAFNETIKIFEEQCQTQERYSKEYIEKFKREGNEKEIQRIMHNHDKLKSRISEIIDSRRRLEEDLKKQAAEYREIDKRMNSIKPDLIQLRKTRDQYLMWLTQKGVRQKKLNEWLGNENTEDQYSLVEDDEDLPHHDEKTWNVGSSNRNKAENLLRGKRDGTFLVRESSKQGCYACSVVVDGEVKHCVINKTATGYGFAEPYNLYSSLKELVLHYQHTSLVQHNDSLNVTLAYPVYAQQRR

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Acetylation	------MSAEGYQYR ------CCCCCCHHH	34.72	-
2	Phosphorylation	------MSAEGYQYR ------CCCCCCHHH	34.72	30635358
6	Phosphorylation	--MSAEGYQYRALYD --CCCCCCHHHHHHC	8.22	29514104
83	Phosphorylation	YIGRKRISPPTPKPR EECCCCCCCCCCCCC	28.94	29472430
86	Phosphorylation	RKRISPPTPKPRPPR CCCCCCCCCCCCCCC	47.53	29472430
150	Phosphorylation	GLECSTLYRTQSSSN CCCCHHHEECCCCCC	16.23	29514104
154	Phosphorylation	STLYRTQSSSNPAEL HHHEECCCCCCHHHH	34.81	21825134
279	Phosphorylation	LFRFPAASSDNTEHL HCCCCCCCCCCHHHH	40.09	-
354	Phosphorylation	LRDTADGTFLVRDAS HHHCCCCCEEEEECC	17.84	28285833
361	Phosphorylation	TFLVRDASTKMHGDY CEEEEECCCCCCCEE	32.61	21825134
362	Phosphorylation	FLVRDASTKMHGDYT EEEEECCCCCCCEEE	33.82	28059163
382	Ubiquitination	GGNNKLIKIFHRDGK CCCCCEEEEEECCCC	50.16	22790023
426	Phosphorylation	KLDVKLLYPVSKYQQ CCCEEEEEECCHHCC	16.42	-
431	Phosphorylation	LLYPVSKYQQDQVVK EEEECCHHCCCCCCC	12.14	26026062
452	Phosphorylation	VGKKLHEYNTQFQEK HHHHHHHHHHHHHHH	16.86	22817900
463	Phosphorylation	FQEKSREYDRLYEEY HHHHHHHHHHHHHHH	12.78	22499769
467	Phosphorylation	SREYDRLYEEYTRTS HHHHHHHHHHHHHHH	13.96	25177544
470	Phosphorylation	YDRLYEEYTRTSQEI HHHHHHHHHHHHHHH	6.92	22499769
471	Phosphorylation	DRLYEEYTRTSQEIQ HHHHHHHHHHHHHHH	30.65	-
504	Phosphorylation	QCQTQERYSKEYIEK HHHHHHHHHHHHHHH	24.28	29514104
505	Phosphorylation	CQTQERYSKEYIEKF HHHHHHHHHHHHHHH	25.82	29514104
508	Phosphorylation	QERYSKEYIEKFKRE HHHHHHHHHHHHHHH	20.37	22499769
556	Phosphorylation	LKKQAAEYREIDKRM HHHHHHHHHHHHHHH	14.60	22817900
576	Phosphorylation	DLIQLRKTRDQYLMW CHHHHHHCHHHHHHH	32.80	26643407
580	Phosphorylation	LRKTRDQYLMWLTQK HHHCHHHHHHHHHHH	11.65	21082442
603	Phosphorylation	EWLGNENTEDQYSLV HHHCCCCCHHHHCCC	34.46	30635358
607	Phosphorylation	NENTEDQYSLVEDDE CCCCHHHHCCCCCCC	19.12	22817900
608	Phosphorylation	ENTEDQYSLVEDDED CCCHHHHCCCCCCCC	21.95	22817900
651	Phosphorylation	GTFLVRESSKQGCYA CCEEEEECCCCCEEE	32.07	30635358
652	Phosphorylation	TFLVRESSKQGCYAC CEEEEECCCCCEEEE	25.01	21825134
657	Phosphorylation	ESSKQGCYACSVVVD ECCCCCEEEEEEEEC	19.91	30635358
679	Phosphorylation	INKTATGYGFAEPYN ECCCCCCCCCCCCCC	12.52	22817900
690	Phosphorylation	EPYNLYSSLKELVLH CCCCHHHHHHHHHHH	29.88	22342344

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
508	Y	Phosphorylation	Kinase	PDGFRB	P05622	PSP
508	Y	Phosphorylation	Kinase	PGFRB	P05622	PhosphoELM
652	S	Phosphorylation	Kinase	PRKD1	Q15139	PSP
690	S	Phosphorylation	Kinase	CHUK	O15111	GPS
690	S	Phosphorylation	Kinase	IKKB	O14920	PSP

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
608	S	Phosphorylation		9312046
Phosphorylated by PIK3CA at Ser-608; phosphorylation is stimulated by insulin and PDGF.

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of P85A_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
CBL_MOUSE	Cbl	physical	9174058
IRS1_MOUSE	Irs1	physical	15380067
IRS2_MOUSE	Irs2	physical	15380067
CBL_MOUSE	Cbl	physical	9013636
GRB10_MOUSE	Grb10	physical	12783867
CBL_MOUSE	Cbl	physical	10940298
CBL_MOUSE	Cbl	physical	10391678
CBL_MOUSE	Cbl	physical	20133944
KIT_MOUSE	Kit	physical	22378847
PSN1_MOUSE	Psen1	physical	15192701
CADH1_MOUSE	Cdh1	physical	15192701
CADH2_MOUSE	Cdh2	physical	15192701
THB_MOUSE	Thrb	physical	17606624
RASH_MOUSE	Hras	physical	24470255
PLAK_MOUSE	Jup	physical	24567360
AKT1_MOUSE	Akt1	physical	24567360
PA2G4_MOUSE	Pa2g4	physical	24651434
JAK2_MOUSE	Jak2	physical	8702385
OSTP_MOUSE	Spp1	physical	25436971
ABI1_MOUSE	Abi1	physical	12515821
CYFP1_MOUSE	Cyfip1	physical	21946561
NCKP1_MOUSE	Nckap1	physical	21946561
CSF1R_MOUSE	Csf1r	physical	8947469
PK3CA_MOUSE	Pik3ca	physical	8947469
CBL_MOUSE	Cbl	physical	8947469
GRB2_MOUSE	Grb2	physical	10995764
SHC1_MOUSE	Shc1	physical	10995764
PTN11_MOUSE	Ptpn11	physical	10995764
RET_MOUSE	Ret	physical	10995764

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of P85A_MOUSE

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Quantitative time-resolved phosphoproteomic analysis of mast cellsignaling."; Cao L., Yu K., Banh C., Nguyen V., Ritz A., Raphael B.J., Kawakami Y.,Kawakami T., Salomon A.R.; J. Immunol. 179:5864-5876(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-467, AND MASSSPECTROMETRY.	17947660 [Abstract]
"Immunoaffinity profiling of tyrosine phosphorylation in cancercells."; Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H.,Zha X.-M., Polakiewicz R.D., Comb M.J.; Nat. Biotechnol. 23:94-101(2005). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-467 AND TYR-607, ANDMASS SPECTROMETRY.	15592455 [Abstract]

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