dbPTM

NEK1_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	NEK1_HUMAN
UniProt AC	Q96PY6
Protein Name	Serine/threonine-protein kinase Nek1
Gene Name	NEK1
Organism	Homo sapiens (Human).
Sequence Length	1258
Subcellular Localization	Nucleus . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cytoplasm . Associated with the pericentriolar material (PubMed:21211617). Localizes to centrosome during interphase and mitosis (By similarity). Translocated from cytopla
Protein Description	Phosphorylates serines and threonines, but also appears to possess tyrosine kinase activity (By similarity). Involved in DNA damage checkpoint control and for proper DNA damage repair. In response to injury that includes DNA damage, NEK1 phosphorylates VDAC1 to limit mitochondrial cell death. [PubMed: 20230784 May be implicated in the control of meiosis (By similarity Involved in cilium assembly]
Protein Sequence	MEKYVRLQKIGEGSFGKAILVKSTEDGRQYVIKEINISRMSSKEREESRREVAVLANMKHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGVLFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYELCTLKHAFEAGSMKNLVLKIISGSFPPVSLHYSYDLRSLVSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTFSKFGSQPIPAKRPASGQNSISVMPAQKITKPAAKYGIPLAYKKYGDKKLHEKKPLQKHKQAHQTPEKRVNTGEERRKISEEAARKRRLEFIEKEKKQKDQIISLMKAEQMKRQEKERLERINRAREQGWRNVLSAGGSGEVKAPFLGSGGTIAPSSFSSRGQYEHYHAIFDQMQQQRAEDNEAKWKREIYGRGLPERGILPGVRPGFPYGAAGHHHFPDADDIRKTLKRLKAVSKQANANRQKGQLAVERAKQVEEFLQRKREAMQNKARAEGHMVYLARLRQIRLQNFNERQQIKAKLRGEKKEANHSEGQEGSEEADMRRKKIESLKAHANARAAVLKEQLERKRKEAYEREKKVWEEHLVAKGVKSSDVSPPLGQHETGGSPSKQQMRSVISVTSALKEVGVDSSLTDTRETSEEMQKTNNAISSKREILRRLNENLKAQEDEKGKQNLSDTFEINVHEDAKEHEKEKSVSSDRKKWEAGGQLVIPLDELTLDTSFSTTERHTVGEVIKLGPNGSPRRAWGKSPTDSVLKILGEAELQLQTELLENTTIRSEISPEGEKYKPLITGEKKVQCISHEINPSAIVDSPVETKSPEFSEASPQMSLKLEGNLEEPDDLETEILQEPSGTNKDESLPCTITDVWISEEKETKETQSADRITIQENEVSEDGVSSTVDQLSDIHIEPGTNDSQHSKCDVDKSVQPEPFFHKVVHSEHLNLVPQVQSVQCSPEESFAFRSHSHLPPKNKNKNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLMDVPTVGDVRQDNLEIDEIEDENIKEGPSDSEDIVFEETDTDLQELQASMEQLLREQPGEEYSEEEESVLKNSDVEPTANGTDVADEDDNPSSESALNEEWHSDNSDGEIASECECDSVFNHLEELRLHLEQEMGFEKFFEVYEKIKAIHEDEDENIEICSKIVQNILGNEHQHLYAKILHLVMADGAYQEDNDE

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
14	Phosphorylation	LQKIGEGSFGKAILV CEEECCCCCCEEEEE	26.90	-
38	Phosphorylation	VIKEINISRMSSKER EEEEEEHHHCCHHHH	19.90	29514088
41	Phosphorylation	EINISRMSSKEREES EEEHHHCCHHHHHHH	36.83	29514088
42	Phosphorylation	INISRMSSKEREESR EEHHHCCHHHHHHHH	29.23	30576142
130	Ubiquitination	KILHRDIKSQNIFLT CCCCCCCCCCCEEEC	50.72	-
138	Ubiquitination	SQNIFLTKDGTVQLG CCCEEECCCCEEEEC	57.20	-
141	Phosphorylation	IFLTKDGTVQLGDFG EEECCCCEEEECHHH	18.40	-
155	Phosphorylation	GIARVLNSTVELART HHHHHHHHHHHHHHH	29.66	19060867
156	Phosphorylation	IARVLNSTVELARTC HHHHHHHHHHHHHHH	19.54	28857561
162	Phosphorylation	STVELARTCIGTPYY HHHHHHHHHCCCCCC	11.67	21082442
207	Phosphorylation	KHAFEAGSMKNLVLK HHHHHHCCCCHHHHH	33.36	27251275
207 (in isoform 3)	Phosphorylation	-	33.36	27251275
209	Ubiquitination	AFEAGSMKNLVLKII HHHHCCCCHHHHHHH	49.15	-
217	Phosphorylation	NLVLKIISGSFPPVS HHHHHHHHCCCCCEE	31.68	26552605
219	Phosphorylation	VLKIISGSFPPVSLH HHHHHHCCCCCEEEE	27.99	26552605
224	Phosphorylation	SGSFPPVSLHYSYDL HCCCCCEEEECCCCH	18.40	26552605
227	Phosphorylation	FPPVSLHYSYDLRSL CCCEEEECCCCHHHH	17.77	26552605
228	Phosphorylation	PPVSLHYSYDLRSLV CCEEEECCCCHHHHH	11.04	26552605
229	Phosphorylation	PVSLHYSYDLRSLVS CEEEECCCCHHHHHH	16.30	26552605
233	Phosphorylation	HYSYDLRSLVSQLFK ECCCCHHHHHHHHHH	40.18	26552605
236	Phosphorylation	YDLRSLVSQLFKRNP CCHHHHHHHHHHHCC	26.85	26552605
251	Phosphorylation	RDRPSVNSILEKGFI CCCCCHHHHHHHCHH	26.79	22617229
285	Phosphorylation	KTFSKFGSQPIPAKR HHHHHHCCCCCCCCC	35.60	28857561
285 (in isoform 3)	Phosphorylation	-	35.60	27251275
291	Acetylation	GSQPIPAKRPASGQN CCCCCCCCCCCCCCC	54.26	25953088
295	Phosphorylation	IPAKRPASGQNSISV CCCCCCCCCCCCCCE	43.52	28555341
299	Phosphorylation	RPASGQNSISVMPAQ CCCCCCCCCCEEECH	14.35	29514088
301	Phosphorylation	ASGQNSISVMPAQKI CCCCCCCCEEECHHC	16.51	29514088
315	Phosphorylation	ITKPAAKYGIPLAYK CCCHHHHHCCCEEEH	18.63	29496907
321	Phosphorylation	KYGIPLAYKKYGDKK HHCCCEEEHHHCCCC	18.72	-
322	Acetylation	YGIPLAYKKYGDKKL HCCCEEEHHHCCCCH	33.63	25953088
344	Phosphorylation	KHKQAHQTPEKRVNT HHHHHHCCCHHCCCC	24.45	17192257
351	Phosphorylation	TPEKRVNTGEERRKI CCHHCCCCHHHHHHH	43.01	21857030
383	Phosphorylation	KQKDQIISLMKAEQM HHHHHHHHHHHHHHH	24.84	27067055
414	Phosphorylation	QGWRNVLSAGGSGEV HHHHHHHHCCCCCEE	22.15	23401153
414 (in isoform 3)	Phosphorylation	-	22.15	24719451
418	Phosphorylation	NVLSAGGSGEVKAPF HHHHCCCCCEEECCE	30.75	29255136
418 (in isoform 3)	Phosphorylation	-	30.75	24719451
428	Phosphorylation	VKAPFLGSGGTIAPS EECCEECCCCEECCC	35.88	21082442
431	Phosphorylation	PFLGSGGTIAPSSFS CEECCCCEECCCCCC	19.93	21406692
435	Phosphorylation	SGGTIAPSSFSSRGQ CCCEECCCCCCCCCC	34.69	24117733
435 (in isoform 3)	Phosphorylation	-	34.69	27251275
436	Phosphorylation	GGTIAPSSFSSRGQY CCEECCCCCCCCCCH	28.26	29507054
438	Phosphorylation	TIAPSSFSSRGQYEH EECCCCCCCCCCHHH	22.52	24117733
439	Phosphorylation	IAPSSFSSRGQYEHY ECCCCCCCCCCHHHH	38.12	24117733
443	Phosphorylation	SFSSRGQYEHYHAIF CCCCCCCHHHHHHHH	13.90	21552520
443 (in isoform 3)	Phosphorylation	-	13.90	27642862
446	Phosphorylation	SRGQYEHYHAIFDQM CCCCHHHHHHHHHHH	4.88	21552520
470	Phosphorylation	AKWKREIYGRGLPER HHHHHHHHCCCCCCC	8.94	19690332
472	Methylation	WKREIYGRGLPERGI HHHHHHCCCCCCCCC	26.89	82954837
528 (in isoform 6)	Phosphorylation	-	7.51	27251275
532	Ubiquitination	QLAVERAKQVEEFLQ CHHHHHHHHHHHHHH	62.18	-
557	Phosphorylation	RAEGHMVYLARLRQI HHHHHHHHHHHHHHH	6.25	-
572 (in isoform 3)	Phosphorylation	-	34.50	27251275
607	Phosphorylation	MRRKKIESLKAHANA HHHHHHHHHHHHHHH	38.35	-
649	Phosphorylation	LVAKGVKSSDVSPPL HHHCCCCCCCCCCCC	29.39	22199227
650	Phosphorylation	VAKGVKSSDVSPPLG HHCCCCCCCCCCCCC	35.99	22199227
653	Phosphorylation	GVKSSDVSPPLGQHE CCCCCCCCCCCCCCC	26.28	23927012
661	Phosphorylation	PPLGQHETGGSPSKQ CCCCCCCCCCCCCHH	44.72	23401153
664	Phosphorylation	GQHETGGSPSKQQMR CCCCCCCCCCHHHHH	27.61	25159151
666	Phosphorylation	HETGGSPSKQQMRSV CCCCCCCCHHHHHHH	44.81	23927012
675	Phosphorylation	QQMRSVISVTSALKE HHHHHHHHHHHHHHH	19.72	28857561
677	Phosphorylation	MRSVISVTSALKEVG HHHHHHHHHHHHHHC	11.07	23312004
678	Phosphorylation	RSVISVTSALKEVGV HHHHHHHHHHHHHCC	29.46	28555341
681 (in isoform 3)	Phosphorylation	-	50.50	24719451
687	Phosphorylation	LKEVGVDSSLTDTRE HHHHCCCCCCCCCHH	25.49	28857561
688	Phosphorylation	KEVGVDSSLTDTRET HHHCCCCCCCCCHHH	30.72	28674419
689 (in isoform 3)	Phosphorylation	-	6.10	24719451
692 (in isoform 3)	Phosphorylation	-	25.77	27251275
703 (in isoform 3)	Phosphorylation	-	42.86	27251275
735	Phosphorylation	GKQNLSDTFEINVHE CCCCCHHCEEEECCH	21.57	27251275
763 (in isoform 3)	Phosphorylation	-	16.92	27251275
798	Phosphorylation	IKLGPNGSPRRAWGK EEECCCCCCCCCCCC	23.13	28102081
805	Methylation	SPRRAWGKSPTDSVL CCCCCCCCCCCHHHH	42.43	115973879
806	Phosphorylation	PRRAWGKSPTDSVLK CCCCCCCCCCHHHHH	29.15	29255136
808	Phosphorylation	RAWGKSPTDSVLKIL CCCCCCCCHHHHHHH	48.25	21815630
810	Phosphorylation	WGKSPTDSVLKILGE CCCCCCHHHHHHHHH	31.65	29255136
826 (in isoform 3)	Phosphorylation	-	9.14	24719451
834	Phosphorylation	LENTTIRSEISPEGE HHCCCCCCCCCCCCC	35.12	23927012
834 (in isoform 3)	Phosphorylation	-	35.12	24719451
837	Phosphorylation	TTIRSEISPEGEKYK CCCCCCCCCCCCCCC	16.91	23401153
838 (in isoform 3)	Phosphorylation	-	60.31	27251275
843	Phosphorylation	ISPEGEKYKPLITGE CCCCCCCCCCCCCCC	17.34	28464451
848	Phosphorylation	EKYKPLITGEKKVQC CCCCCCCCCCCEEEE	47.15	23927012
857	Phosphorylation	EKKVQCISHEINPSA CCEEEEEECCCCHHH	23.77	30266825
863	Phosphorylation	ISHEINPSAIVDSPV EECCCCHHHHCCCCC	26.58	30266825
865 (in isoform 3)	Phosphorylation	-	4.08	24719451
868	Phosphorylation	NPSAIVDSPVETKSP CHHHHCCCCCCCCCC	21.68	30266825
872	Phosphorylation	IVDSPVETKSPEFSE HCCCCCCCCCCCHHH	36.74	30266825
874	Phosphorylation	DSPVETKSPEFSEAS CCCCCCCCCCHHHCC	36.76	29255136
878	Phosphorylation	ETKSPEFSEASPQMS CCCCCCHHHCCCCCE	30.50	25850435
881	Phosphorylation	SPEFSEASPQMSLKL CCCHHHCCCCCEEEE	16.18	25159151
885	Phosphorylation	SEASPQMSLKLEGNL HHCCCCCEEEEECCC	19.66	29978859
896 (in isoform 3)	Phosphorylation	-	76.14	24719451
907	Phosphorylation	TEILQEPSGTNKDES HHHHCCCCCCCCCCC	58.45	26074081
909	Phosphorylation	ILQEPSGTNKDESLP HHCCCCCCCCCCCCC	43.35	26074081
909 (in isoform 3)	Phosphorylation	-	43.35	27251275
914	Phosphorylation	SGTNKDESLPCTITD CCCCCCCCCCEEEEE	47.98	26074081
918	Phosphorylation	KDESLPCTITDVWIS CCCCCCEEEEEEEEC	25.60	26074081
920	Phosphorylation	ESLPCTITDVWISEE CCCCEEEEEEEECCC	13.32	26074081
925	Phosphorylation	TITDVWISEEKETKE EEEEEEECCCCCCCC	24.80	29507054
930	Phosphorylation	WISEEKETKETQSAD EECCCCCCCCCCCCC	45.29	26074081
952	Phosphorylation	EVSEDGVSSTVDQLS CCCCCCCCCCHHHHH	26.31	26471730
953	Phosphorylation	VSEDGVSSTVDQLSD CCCCCCCCCHHHHHC	30.00	26471730
953 (in isoform 3)	Phosphorylation	-	30.00	27251275
954	Phosphorylation	SEDGVSSTVDQLSDI CCCCCCCCHHHHHCC	22.16	26471730
959	Phosphorylation	SSTVDQLSDIHIEPG CCCHHHHHCCEECCC	28.96	26471730
967	Phosphorylation	DIHIEPGTNDSQHSK CCEECCCCCCCCCCC	46.71	22210691
970	Phosphorylation	IEPGTNDSQHSKCDV ECCCCCCCCCCCCCC	31.68	26471730
973	Phosphorylation	GTNDSQHSKCDVDKS CCCCCCCCCCCCCCC	27.60	26471730
980	Phosphorylation	SKCDVDKSVQPEPFF CCCCCCCCCCCCCCC	22.86	28555341
1004	Phosphorylation	NLVPQVQSVQCSPEE CCCCCCEEEECCHHH	18.46	28464451
1008	Phosphorylation	QVQSVQCSPEESFAF CCEEEECCHHHCCCC	20.46	28464451
1012	Phosphorylation	VQCSPEESFAFRSHS EECCHHHCCCCCCCC	21.88	29978859
1030	Phosphorylation	PKNKNKNSLLIGLST CCCCCCCCEEEEECC	26.60	27251275
1032 (in isoform 3)	Phosphorylation	-	2.68	24719451
1036	Phosphorylation	NSLLIGLSTGLFDAN CCEEEEECCCCCCCC	18.40	27050516
1050	Phosphorylation	NNPKMLRTCSLPDLS CCHHHHCCCCCCHHH	11.50	19664994
1052	Phosphorylation	PKMLRTCSLPDLSKL HHHHCCCCCCHHHHH	42.18	25159151
1057	Phosphorylation	TCSLPDLSKLFRTLM CCCCCHHHHHHHHHC	34.39	30266825
1058 (in isoform 3)	Phosphorylation	-	59.97	27251275
1080 (in isoform 3)	Phosphorylation	-	41.25	24719451
1092	Phosphorylation	ENIKEGPSDSEDIVF CCCCCCCCCHHHCEE	65.70	22817901
1094	Phosphorylation	IKEGPSDSEDIVFEE CCCCCCCHHHCEEEC	41.32	27732954
1102	Phosphorylation	EDIVFEETDTDLQEL HHCEEECCCCCHHHH	36.68	30301811
1104	Phosphorylation	IVFEETDTDLQELQA CEEECCCCCHHHHHH	46.13	30301811
1122 (in isoform 3)	Phosphorylation	-	39.81	27251275
1125	Phosphorylation	REQPGEEYSEEEESV HHCCCCCCCHHHHHH	19.65	30278072
1126	Phosphorylation	EQPGEEYSEEEESVL HCCCCCCCHHHHHHH	41.64	23927012
1131	Phosphorylation	EYSEEEESVLKNSDV CCCHHHHHHHHCCCC	35.71	23927012
1154 (in isoform 3)	Phosphorylation	-	46.34	24719451
1225	Ubiquitination	ENIEICSKIVQNILG CCHHHHHHHHHHHHC	42.35	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
14	S	Phosphorylation	Kinase	TLK1	Q9UKI8	PSP
141	T	Phosphorylation	Kinase	TLK1	Q9UKI8	PSP
315	Y	Phosphorylation	Kinase	TLK1	Q9UKI8	PSP

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of NEK1_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of NEK1_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
ZN350_HUMAN	ZNF350	physical	14690447
FEZ1_HUMAN	FEZ1	physical	14690447
FEZ2_HUMAN	FEZ2	physical	14690447
1433F_HUMAN	YWHAH	physical	14690447
ATRX_HUMAN	ATRX	physical	14690447
TP53B_HUMAN	TP53BP1	physical	14690447
MRE11_HUMAN	MRE11A	physical	14690447
KIF3A_HUMAN	KIF3A	physical	14690447
2A5A_HUMAN	PPP2R5A	physical	14690447
TSC2_HUMAN	TSC2	physical	14690447
CTNL1_HUMAN	CTNNAL1	physical	14690447
VTNC_HUMAN	VTN	physical	27173435
FTM_HUMAN	RPGRIP1L	physical	27173435
SPAT7_HUMAN	SPATA7	physical	27173435

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
263520	Short-rib thoracic dysplasia 6 with or without polydactyly (SRTD6)
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of NEK1_HUMAN

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Quantitative phosphoproteomic analysis of T cell receptor signalingreveals system-wide modulation of protein-protein interactions."; Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K.,Rodionov V., Han D.K.; Sci. Signal. 2:RA46-RA46(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1126, AND MASSSPECTROMETRY.	19690332 [Abstract]
"Large-scale proteomics analysis of the human kinome."; Oppermann F.S., Gnad F., Olsen J.V., Hornberger R., Greff Z., Keri G.,Mann M., Daub H.; Mol. Cell. Proteomics 8:1751-1764(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-155; THR-344; SER-414;SER-418; SER-428; SER-653; THR-661; SER-798; SER-806; SER-834;SER-837; SER-868; SER-881; SER-1008; SER-1052; TYR-1125 AND SER-1126,AND MASS SPECTROMETRY.	19369195 [Abstract]
"Lys-N and trypsin cover complementary parts of the phosphoproteome ina refined SCX-based approach."; Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J.,Mohammed S.; Anal. Chem. 81:4493-4501(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-881 AND SER-1126, ANDMASS SPECTROMETRY.	19413330 [Abstract]
"Kinase-selective enrichment enables quantitative phosphoproteomics ofthe kinome across the cell cycle."; Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R.,Greff Z., Keri G., Stemmann O., Mann M.; Mol. Cell 31:438-448(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-344; SER-837; SER-1052AND SER-1126, AND MASS SPECTROMETRY.	18691976 [Abstract]