FBXW7_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: FBXW7_MOUSE
• UniProt AC: Q8VBV4
• Protein Name: F-box/WD repeat-containing protein 7 {ECO:0000305}
• Gene Name: Fbxw7 {ECO:0000312|MGI:MGI:1354695}
• Organism: Mus musculus (Mouse).
• Sequence Length: 710
• Subcellular Localization: Nucleus, nucleoplasm .
• Protein Description: Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. [PubMed: 21953459 Recognizes and binds phosphorylated sites/phosphodegrons within target proteins and thereafter bring them to the SCF complex for ubiquitination (By similarity Identified substrates include cyclin-E (CCNE1 or CCNE2), JUN, MYC, NOTCH1 released notch intracellular domain (NICD), NOTCH2, MCL1 and probably PSEN1 (By similarity Acts as a negative regulator of JNK signaling by binding to phosphorylated JUN and promoting its ubiquitination and subsequent degradation (By similarity SCF(FBXW7) complex mediates the ubiquitination and subsequent degradation of NFE2L1]
• Protein Sequence: MNQELLSVGSKRRRTGGSLRGNASSSQVDEGQMNRVVEEDPQQQARHQEEEHTARNGELVGANPRPGDQNDTQQGQVEENNNRFISVDEDSSGNQEEQEEDEEHAGEQEEEEEEEEEEEEMDQESDDFDPSDDSSREDEHTHNSNVTNCSSVSDLPAHQLSSPFYTKTTKMKRKLDHGSEVRSFSLGKKPCKVSDYTSTTGLVPCSATPTTFGDLRAANGQGQQRRRITSVQPPTGLQEWLKMFQSWSGPEKLLALDELIDSCEPTQVKHMMQVIEPQFQRDFISLLPKELALYVLSFLEPKDLLQAAQTCRYWRILAEDNLLWREKCKEEGIDEPLHIKRRKIIKPGFIHSPWKSAYIRQHRIDTNWRRGELKSPKVLKGHDDHVITCLQFCGNRIVSGSDDNTLKVWSAVTGKCLRTLVGHTGGVWSSQMRDNIIISGSTDRTLKVWNAETGECIHTLYGHTSTVRCMHLHEKRVVSGSRDATLRVWDIETGQCLHVLMGHVAAVRCVQYDGRRVVSGAYDFMVKVWDPETETCLHTLQGHTNRVYSLQFDGIHVVSGSLDTSIRVWDVETGNCIHTLTGHQSLTSGMELKDNILVSGNADSTVKIWDIKTGQCLQTLQGPSKHQSAVTCLQFNKNFVITSSDDGTVKLWDLKTGEFIRNLVTLESGGSGGVVWRIRASNTKLVCAVGSRNGTEETKLLVLDFDVDMK

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
7	Phosphorylation	-MNQELLSVGSKRRR -CCHHHHCCCCCCCC	16.81	-
7	Phosphorylation	-MNQELLSVGSKRRR -CCHHHHCCCCCCCC	16.81	19367708
10	Phosphorylation	QELLSVGSKRRRTGG HHHHCCCCCCCCCCC	3.29	-
10	Phosphorylation	QELLSVGSKRRRTGG HHHHCCCCCCCCCCC	3.29	19367708
127	Phosphorylation	EMDQESDDFDPSDDS HHHHCCCCCCCCCCC	12.60	-
149	Phosphorylation	HNSNVTNCSSVSDLP CCCCCCCCCCHHHCC	19.18	-
208	Phosphorylation	GLVPCSATPTTFGDL CEEECCCCCCCHHHH	66.39	-
230	Phosphorylation	QQRRRITSVQPPTGL CCCCCCCCCCCCCCH	1.77	-
285	Phosphorylation	QFQRDFISLLPKELA HHHHHHHHCCHHHHH	34.02	30635358
294	Phosphorylation	LPKELALYVLSFLEP CHHHHHHHHHHHCCH	42.45	-
343	Phosphorylation	PLHIKRRKIIKPGFI CCHHCCCCCCCCCCC	29.69	28576409
358	Phosphorylation	HSPWKSAYIRQHRID CCCCHHHHHHHHCCC	19.11	23984901
360	Phosphorylation	PWKSAYIRQHRIDTN CCHHHHHHHHCCCCC	21.97	23984901
361	Phosphorylation	WKSAYIRQHRIDTNW CHHHHHHHHCCCCCC	32.78	23984901
375	Phosphorylation	WRRGELKSPKVLKGH CCCCCCCCCHHHCCC	2.67	24719451

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
230	S	Phosphorylation	Kinase	SGK1	Q9WVC6	Uniprot

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
208	T	Phosphorylation	Phosphorylation at Thr-208 promotes interaction with PIN1, leading to disrupt FBXW7 dimerization and promoting FBXW7 autoubiquitination and degradation.	-
208	T	Phosphorylation	Ubiquitinated: autoubiquitinates following phosphorylation at Thr-208 and subsequent interaction with PIN1 (By similarity).	22748924
208	T	ubiquitylation	Phosphorylation at Thr-208 promotes interaction with PIN1, leading to disrupt FBXW7 dimerization and promoting FBXW7 autoubiquitination and degradation.	-
208	T	ubiquitylation	Ubiquitinated: autoubiquitinates following phosphorylation at Thr-208 and subsequent interaction with PIN1 (By similarity).	22748924

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of FBXW7_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
PIN1_MOUSE	Pin1	physical	22608923
MCL1_MOUSE	Mcl1	physical	21368834
CEBPA_HUMAN	CEBPA	physical	20534483
KPCE_MOUSE	Prkce	physical	20815813
KPCA_MOUSE	Prkca	physical	20815813
KPCB_MOUSE	Prkcb	physical	20815813
KPCD_MOUSE	Prkcd	physical	20815813
KPCZ_MOUSE	Prkcz	physical	20815813
CUL1_MOUSE	Cul1	physical	11735228
SKP1_MOUSE	Skp1a	physical	11735228
CR3L1_HUMAN	CREB3L1	physical	23955342
SKP1_MOUSE	Skp1a	physical	23955342
CR3L2_HUMAN	CREB3L2	physical	23955342
GATA2_MOUSE	Gata2	physical	25670854
SKP1_MOUSE	Skp1a	physical	25670854
CUL1_MOUSE	Cul1	physical	25670854
MYC_MOUSE	Myc	physical	25670854
MYB_MOUSE	Myb	physical	25670854
RUNX2_MOUSE	Runx2	physical	26542806
GSK3B_MOUSE	Gsk3b	physical	26542806
PRGC1_MOUSE	Ppargc1a	physical	25918410
MYB_HUMAN	MYB	physical	21092141
MYB_MOUSE	Myb	physical	21092141

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.