ATG5_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: ATG5_HUMAN
• UniProt AC: Q9H1Y0
• Protein Name: Autophagy protein 5
• Gene Name: ATG5
• Organism: Homo sapiens (Human).
• Sequence Length: 275
• Subcellular Localization: Cytoplasm . Preautophagosomal structure membrane; Peripheral membrane protein. Colocalizes with nonmuscle actin. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed (By similarity). Localizes also
• Protein Description: Involved in autophagic vesicle formation. Conjugation with ATG12, through a ubiquitin-like conjugating system involving ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. Involved in mitochondrial quality control after oxidative damage, and in subsequent cellular longevity. Plays a critical role in multiple aspects of lymphocyte development and is essential for both B and T lymphocyte survival and proliferation. Required for optimal processing and presentation of antigens for MHC II. Involved in the maintenance of axon morphology and membrane structures, as well as in normal adipocyte differentiation. Promotes primary ciliogenesis through removal of OFD1 from centriolar satellites and degradation of IFT20 via the autophagic pathway.; May play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. Plays a crucial role in IFN-gamma-induced autophagic cell death by interacting with FADD.; (Microbial infection) May act as a proviral factor. In association with ATG12, negatively regulates the innate antiviral immune response by impairing the type I IFN production pathway upon vesicular stomatitis virus (VSV) infection. [PubMed: 17709747 Required for the translation of incoming hepatitis C virus (HCV) RNA and, thereby, for initiation of HCV replication, but not required once infection is established]
• Protein Sequence: MTDDKDVLRDVWFGRIPTCFTLYQDEITEREAEPYYLLLPRVSYLTLVTDKVKKHFQKVMRQEDISEIWFEYEGTPLKWHYPIGLLFDLLASSSALPWNITVHFKSFPEKDLLHCPSKDAIEAHFMSCMKEADALKHKSQVINEMQKKDHKQLWMGLQNDRFDQFWAINRKLMEYPAEENGFRYIPFRIYQTTTERPFIQKLFRPVAADGQLHTLGDLLKEVCPSAIDPEDGEKKNQVMIHGIEPMLETPLQWLSEHLSYPDNFLHISIIPQPTD

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
1	Acetylation	-------MTDDKDVL -------CCCHHHHH	9.28	22814378
5	Ubiquitination	---MTDDKDVLRDVW ---CCCHHHHHHHHH	53.17	-
35	Phosphorylation	TEREAEPYYLLLPRV CCCCCCCEEEECCCC	9.76	30576142
36	Phosphorylation	EREAEPYYLLLPRVS CCCCCCEEEECCCCC	11.11	21214269
49	Phosphorylation	VSYLTLVTDKVKKHF CCHHHHCHHHHHHHH	32.96	30576142
60 (in isoform 2)	Ubiquitination	-	4.76	21906983
75	Phosphorylation	IWFEYEGTPLKWHYP HHCEECCCCCEECCC	17.32	-
81	Phosphorylation	GTPLKWHYPIGLLFD CCCCEECCCHHHHHH	8.74	-
93 (in isoform 2)	Ubiquitination	-	17.64	21906983
101	Phosphorylation	SALPWNITVHFKSFP CCCCCEEEEEECCCC	12.52	-
138	Ubiquitination	EADALKHKSQVINEM HHHHHHHHHHHHHHH	40.78	21906983
138 (in isoform 1)	Ubiquitination	-	40.78	21906983
147	Ubiquitination	QVINEMQKKDHKQLW HHHHHHHHHHHHHHH	59.88	-
171	Ubiquitination	QFWAINRKLMEYPAE HHHHHHHHHHCCCHH	47.93	2190698
171 (in isoform 1)	Ubiquitination	-	47.93	21906983
214	Phosphorylation	AADGQLHTLGDLLKE CCCCCEEEHHHHHHH	40.82	27307780
220	Ubiquitination	HTLGDLLKEVCPSAI EEHHHHHHHHCCCCC	56.23	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
101	T	Phosphorylation	Kinase	PAK1	Q13153	PSP

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of ATG5_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of ATG5_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
IMDH2_HUMAN	IMPDH2	physical	17353931
A16L1_HUMAN	ATG16L1	physical	20562859
TCPR1_HUMAN	TECPR1	physical	20562859
ATG12_HUMAN	ATG12	physical	20562859
TKT_HUMAN	TKT	physical	20562859
SIR1_HUMAN	SIRT1	physical	18296641
TCPR1_HUMAN	TECPR1	physical	22342342
A16L1_HUMAN	ATG16L1	physical	22342342
ATG12_HUMAN	ATG12	physical	22342342
WDFY3_HUMAN	WDFY3	physical	20417604
A16L1_HUMAN	ATG16L1	physical	22863883
CAV1_HUMAN	CAV1	physical	24727585
FADD_HUMAN	FADD	physical	15778222
KEAP1_HUMAN	KEAP1	physical	25416956
CCHCR_HUMAN	CCHCR1	physical	25416956
TEKT4_HUMAN	TEKT4	physical	25416956
A16L1_HUMAN	ATG16L1	physical	23202584
A16L1_HUMAN	ATG16L1	physical	26344197
TCPR1_HUMAN	TECPR1	physical	25484072
A16L1_HUMAN	ATG16L1	physical	25484072
SQSTM_HUMAN	SQSTM1	physical	27879200
CACO2_HUMAN	CALCOCO2	physical	27879200
OPTN_HUMAN	OPTN	physical	27879200

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• There are no disease associations of PTM sites.
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.