AHK4_ARATH - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: AHK4_ARATH
• UniProt AC: Q9C5U0
• Protein Name: Histidine kinase 4
• Gene Name: AHK4
• Organism: Arabidopsis thaliana (Mouse-ear cress).
• Sequence Length: 1080
• Subcellular Localization: Endoplasmic reticulum membrane ; Multi-pass membrane protein .
• Protein Description: Cytokinins (CK) receptor related to bacterial two-component regulators. Binds also the synthetic urea-type cytokinin thiadiazuron, a potent defoliant and herbicide. Functions as a histidine kinase and transmits the stress signal to a downstream MAPK cascade. This protein undergoes an ATP-dependent autophosphorylation at a conserved histidine residue in the kinase core, and a phosphoryl group is then transferred to a conserved aspartate residue in the receiver domain. In the presence of cytokinin, feeds phosphate to phosphorelay-integrating histidine phosphotransfer protein (HPt) and activates subsequent cascade. In the absence of cytokinin, removes phosphate from HPt proteins, decreasing the system phosphoload. Involved in meristems establishment in seedlings. Acts as a redundant negative regulator of drought and salt stress responses, and abscisic acid (ABA) signaling in a cytokinin-dependent manner. Required to set vascular asymmetric cell divisions that establish phloem and procambium cell lines. Redundant positive regulator of cytokinin signaling that regulates many developmental processes including seed germination, cell division, seed size, chlorophyll retention during leaf senescence, root repression and shoot promotion. Can interact with isoprenoid-type cytokinins trans-zeatin (tZ and tZR), isopentenyladenine (iP), and isopentenyladenosine (iPR), the meta hydroxylated derivative of benzyladenine m-topolin, buta-2,3-dienyladenine (HA-8), penta-2,3-dienyladenine (HA-1), 4-methyl-penta-2,3-dienyladenine (HA-10), 4-hydroxy-2-butynyladenine (RM1), 2-butynyladenine (RM6), and to a lower extent, with cis-zeatin (cZ), zeatin riboside and dihydrozeatin (DZ). Together with AHK3, involved in the cytokinin-dependent responses to Pi starvation and sucrose stresses. Required for the formation of auxin-transporting vascular tissues in the hypocotyl, and primary and lateral roots, but not in adventitious roots, thus leading to auxin basipetal transport that regulates root development and branching. Involved in alkamides (e.g. N-isobutyl decanamide) and N-acylethanolamides (NAE) signaling that control meristematic activity and differentiation processes during plant development. Prevents the uptake of sulfate by mediating cytokinin-dependent down-regulation of high-affinity sulfate transporters (e.g. SULTR1;1 and SULTR1;2) expression in roots. Together with AHK2, required for growth and reproduction promotion stimulated by the endophytic fungus Piriformospora indica in a trans-zeatin-dependent manner. Required to trigger the phytotoxic effect of the snapdragon (Antirrhinum majus) flowers volatile organic compound (VOC) methyl benzoate (MB). Plays a role in the cytokinin-mediated repression of the iron uptake pathway..
• Protein Sequence: MRRDFVYNNNAMFNPLTTHYSSDMNWALNNHQEEEEEPRRIEISDSESLENLKSSDFYQLGGGGALNSSEKPRKIDFWRSGLMGFAKMQQQQQLQHSVAVKMNNNNNNDLMGNKKGSTFIQEHRALLPKALILWIIIVGFISSGIYQWMDDANKIRREEVLVSMCDQRARMLQDQFSVSVNHVHALAILVSTFHYHKNPSAIDQETFAEYTARTAFERPLLSGVAYAEKVVNFEREMFERQHNWVIKTMDRGEPSPVRDEYAPVIFSQDSVSYLESLDMMSGEEDRENILRARETGKAVLTSPFRLLETHHLGVVLTFPVYKSSLPENPTVEERIAATAGYLGGAFDVESLVENLLGQLAGNQAIVVHVYDITNASDPLVMYGNQDEEADRSLSHESKLDFGDPFRKHKMICRYHQKAPIPLNVLTTVPLFFAIGFLVGYILYGAAMHIVKVEDDFHEMQELKVRAEAADVAKSQFLATVSHEIRTPMNGILGMLAMLLDTELSSTQRDYAQTAQVCGKALIALINEVLDRAKIEAGKLELESVPFDIRSILDDVLSLFSEESRNKSIELAVFVSDKVPEIVKGDSGRFRQIIINLVGNSVKFTEKGHIFVKVHLAEQSKDESEPKNALNGGVSEEMIVVSKQSSYNTLSGYEAADGRNSWDSFKHLVSEEQSLSEFDISSNVRLMVSIEDTGIGIPLVAQGRVFMPFMQADSSTSRNYGGTGIGLSISKCLVELMRGQINFISRPHIGSTFWFTAVLEKCDKCSAINHMKKPNVEHLPSTFKGMKAIVVDAKPVRAAVTRYHMKRLGINVDVVTSLKTAVVAAAAFERNGSPLPTKPQLDMILVEKDSWISTEDNDSEIRLLNSRTNGNVHHKSPKLALFATNITNSEFDRAKSAGFADTVIMKPLRASMIGACLQQVLELRKTRQQHPEGSSPATLKSLLTGKKILVVDDNIVNRRVAAGALKKFGAEVVCAESGQVALGLLQIPHTFDACFMDIQMPQMDGFEATRQIRMMEKETKEKTNLEWHLPILAMTADVIHATYEECLKSGMDGYVSKPFEEENLYKSVAKSFKPNPISPSS

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
177	Phosphorylation	RMLQDQFSVSVNHVH HHHHHCCCHHHHHHH	14.09	28011693
179	Phosphorylation	LQDQFSVSVNHVHAL HHHCCCHHHHHHHHH	18.92	28011693
482	Phosphorylation	QFLATVSHEIRTPMN HHHHHHCHHHCCCCH	30.11	16753566
780	Phosphorylation	PNVEHLPSTFKGMKA CCHHCCCCCCCCCEE	53.49	19880383
781	Phosphorylation	NVEHLPSTFKGMKAI CHHCCCCCCCCCEEE	27.68	19880383
910	Phosphorylation	IMKPLRASMIGACLQ CHHHHHHHHHHHHHH	12.42	23776212
996	Phosphorylation	TFDACFMDIQMPQMD CCCEEEECCCCCCCC	14.02	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of AHK4_ARATH !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of AHK4_ARATH !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of AHK4_ARATH !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
AHP2_ARATH	AHP2	physical	18642946
WNK5_ARATH	WNK5	physical	18642946
R15A1_ARATH	AT5G59850	physical	18642946
RS81_ARATH	AT5G20290	physical	18642946
BLH4_ARATH	BLH4	physical	18642946
AMPD_ARATH	FAC1	physical	18642946
E136_ARATH	AT5G58090	physical	18642946
G3PP1_ARATH	GAPCP-1	physical	18642946
G3PC2_ARATH	GAPC2	physical	18642946
ATP5E_ARATH	AT1G51650	physical	18642946
PIP12_ARATH	PIP1B	physical	18642946
GNOM_ARATH	GN	physical	18642946
ALMT1_ARATH	ALMT1	physical	18642946
LSH1_ARATH	LSH1	physical	18642946
REMO_ARATH	AT2G45820	physical	18642946
FK132_ARATH	AT5G60570	physical	18642946
CSPLO_ARATH	AT4G15630	physical	18642946
AHP1_ARATH	AHP1	physical	16965536
AHP2_ARATH	AHP2	physical	16965536
AHP3_ARATH	AHP3	physical	16965536
AHP5_ARATH	AHP5	physical	16965536

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.