T2AG_YEAST - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: T2AG_YEAST
• UniProt AC: P32774
• Protein Name: Transcription initiation factor IIA subunit 2
• Gene Name: TOA2
• Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
• Sequence Length: 122
• Subcellular Localization: Cytoplasm . Nucleus . Imported to nucleus via interaction with KAP122.
• Protein Description: TFIIA is a component of the transcription machinery of RNA polymerase II and plays an important role in transcriptional activation. TFIIA in a complex with TBP mediates transcriptional activity..
• Protein Sequence: MAVPGYYELYRRSTIGNSLVDALDTLISDGRIEASLAMRVLETFDKVVAETLKDNTQSKLTVKGNLDTYGFCDDVWTFIVKNCQVTVEDSHRDASQNGSGDSQSVISVDKLRIVACNSKKSE

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
6	Phosphorylation	--MAVPGYYELYRRS --CCCCCCHHHHHCC	6.41	30377154
7	Phosphorylation	-MAVPGYYELYRRST -CCCCCCHHHHHCCC	12.84	30377154
43	Phosphorylation	LAMRVLETFDKVVAE HHHHHHHHHHHHHHH	32.73	30377154
46	Acetylation	RVLETFDKVVAETLK HHHHHHHHHHHHHHC	34.12	24489116
53	Ubiquitination	KVVAETLKDNTQSKL HHHHHHHCCCCCCCE	57.25	23749301
86	Phosphorylation	IVKNCQVTVEDSHRD EEECCEEEEEECCCC	7.72	24961812
90	Phosphorylation	CQVTVEDSHRDASQN CEEEEEECCCCHHHC	13.60	28889911
95	Phosphorylation	EDSHRDASQNGSGDS EECCCCHHHCCCCCC	28.59	22369663
99	Phosphorylation	RDASQNGSGDSQSVI CCHHHCCCCCCCCEE	47.15	22369663
102	Phosphorylation	SQNGSGDSQSVISVD HHCCCCCCCCEEEEE	28.07	22369663
104	Phosphorylation	NGSGDSQSVISVDKL CCCCCCCCEEEEEEE	25.93	22369663
107	Phosphorylation	GDSQSVISVDKLRIV CCCCCEEEEEEEEEE	23.31	22369663

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of T2AG_YEAST !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of T2AG_YEAST !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of T2AG_YEAST !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
TBP_YEAST	SPT15	physical	11223526
TF2B_YEAST	SUA7	physical	11223526
TAF11_YEAST	TAF11	physical	11238911
TOA1_YEAST	TOA1	physical	11238911
TBP_YEAST	SPT15	physical	12527429
SPT3_YEAST	SPT3	genetic	15132995
SPT8_YEAST	SPT8	genetic	15132995
GCN5_YEAST	GCN5	genetic	15340090
SNF2_YEAST	SNF2	genetic	15340090
RRT5_YEAST	RRT5	physical	16554755
RT18_YEAST	MRPS18	physical	16554755
YO093_YEAST	YOR093C	physical	16554755
TOA1_YEAST	TOA1	physical	16554755
AP1_YEAST	YAP1	physical	16835452
TBP_YEAST	SPT15	genetic	15340090
DBP5_YEAST	DBP5	genetic	22963203
TAF4_YEAST	TAF4	physical	23814059
TOA1_YEAST	TOA1	genetic	23814059
TAF11_YEAST	TAF11	genetic	23814059
TAF4_YEAST	TAF4	genetic	23814059

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"A multidimensional chromatography technology for in-depthphosphoproteome analysis.";
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
Mol. Cell. Proteomics 7:1389-1396(2008).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-95; SER-99; SER-102;SER-104 AND SER-107, AND MASS SPECTROMETRY.
PubMed: 18407956

"Proteome-wide identification of in vivo targets of DNA damagecheckpoint kinases.";
Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H.;
Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-99 AND SER-102, AND MASSSPECTROMETRY.
PubMed: 17563356