dbPTM

STX1A_RAT - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	STX1A_RAT
UniProt AC	P32851
Protein Name	Syntaxin-1A
Gene Name	Stx1a
Organism	Rattus norvegicus (Rat).
Sequence Length	288
Subcellular Localization	Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane Single-pass type IV membrane protein . Cell membrane . Cell junction, synapse, synaptosome . Colocalizes with KCNB1 at the cell membrane (PubMed:17301173). Colocalizes with PLCL1 at
Protein Description	Plays a role in hormone and neurotransmitter exocytosis. [PubMed: 17301173]
Protein Sequence	MKDRTQELRTAKDSDDDDDVTVTVDRDRFMDEFFEQVEEIRGFIDKIAENVEEVKRKHSAILASPNPDEKTKEELEELMSDIKKTANKVRSKLKSIEQSIEQEEGLNRSSADLRIRKTQHSTLSRKFVEVMSEYNATQSDYRERCKGRIQRQLEITGRTTTSEELEDMLESGNPAIFASGIIMDSSISKQALSEIETRHSEIIKLENSIRELHDMFMDMAMLVESQGEMIDRIEYNVEHAVDYVERAVSDTKKAVKYQSKARRKKIMIIICCVILGIIIASTIGGIFG

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
10	Phosphorylation	DRTQELRTAKDSDDD HHHHHHHHCCCCCCC	51.16	30240740
14	Phosphorylation	ELRTAKDSDDDDDVT HHHHCCCCCCCCCCE	42.08	21187092
21	Phosphorylation	SDDDDDVTVTVDRDR CCCCCCCEEEEEHHH	19.90	25403869
23	Phosphorylation	DDDDVTVTVDRDRFM CCCCCEEEEEHHHHH	13.81	28432305
46	Acetylation	EIRGFIDKIAENVEE HHHHHHHHHHHCHHH	38.71	22902405
46	Ubiquitination	EIRGFIDKIAENVEE HHHHHHHHHHHCHHH	38.71	-
59	Phosphorylation	EEVKRKHSAILASPN HHHHHHHHHHHCCCC	21.75	28432305
64	Phosphorylation	KHSAILASPNPDEKT HHHHHHCCCCCCHHH	23.05	30411139
83	Acetylation	EELMSDIKKTANKVR HHHHHHHHHHHHHHH	49.41	22902405
88	Acetylation	DIKKTANKVRSKLKS HHHHHHHHHHHHHHH	36.24	22902405
92	Acetylation	TANKVRSKLKSIEQS HHHHHHHHHHHHHHH	49.74	22902405
94	Ubiquitination	NKVRSKLKSIEQSIE HHHHHHHHHHHHHHH	53.80	-
95	Phosphorylation	KVRSKLKSIEQSIEQ HHHHHHHHHHHHHHH	41.62	25403869
99	Phosphorylation	KLKSIEQSIEQEEGL HHHHHHHHHHHHHCC	18.46	22673903
109	Phosphorylation	QEEGLNRSSADLRIR HHHCCCCCHHHHHHH	28.89	25403869
110	Phosphorylation	EEGLNRSSADLRIRK HHCCCCCHHHHHHHH	23.67	25403869
122	Phosphorylation	IRKTQHSTLSRKFVE HHHHHCCHHHHHHHH	26.99	25403869
159	Phosphorylation	QLEITGRTTTSEELE HHHHHCCCCCHHHHH	35.45	22673903
160	Phosphorylation	LEITGRTTTSEELED HHHHCCCCCHHHHHH	27.28	22673903
161	Phosphorylation	EITGRTTTSEELEDM HHHCCCCCHHHHHHH	33.26	22673903
162	Phosphorylation	ITGRTTTSEELEDML HHCCCCCHHHHHHHH	27.29	22817900
188	Phosphorylation	IIMDSSISKQALSEI EEECCCHHHHHHHHH	22.21	22817900
200	Phosphorylation	SEIETRHSEIIKLEN HHHHHHHHHHHHHHH	27.48	25403869
256	Ubiquitination	SDTKKAVKYQSKARR HHHHHHHHHHHHHHH	42.29	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
14	S	Phosphorylation	Kinase	CK2A1	P68400	PSP
14	S	Phosphorylation	Kinase	CSNK2A1	P19139	GPS
21	T	Phosphorylation	Kinase	CSNK1A1	P48729	GPS
188	S	Phosphorylation	Kinase	DAPK1	P53355	PSP
188	S	Phosphorylation	Kinase	DAPK1	-	PhosphoELM

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Reference
188	S	Phosphorylation	12730201
Phosphorylation at Ser-188 by DAPK1 significantly decreases its interaction with STXBP1 (By similarity).
188	S	Phosphorylation	12730201
Phosphorylation at Ser-188 by DAPK1 significantly decreases its interaction with STXBP1.

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of STX1A_RAT !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
STXB1_RAT	Stxbp1	physical	8108429
STXB1_RAT	Stxbp1	physical	21266332
SNP25_RAT	Snap25	physical	21266332
VAMP2_RAT	Vamp2	physical	21266332
SNP25_MOUSE	Snap25	physical	7768895
STXB1_RAT	Stxbp1	physical	7768895
STXB2_RAT	Stxbp2	physical	7768895
STXB1_RAT	Stxbp1	physical	8996080
HGS_RAT	Hgs	physical	10825299
SNP25_RAT	Snap25	physical	10825299
KCNB1_RAT	Kcnb1	physical	22411134
STX1A_RAT	Stx1a	physical	22411134
STXB1_RAT	Stxbp1	physical	22411134
SNP25_RAT	Snap25	physical	22411134
VAMP2_RAT	Vamp2	physical	22411134

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of STX1A_RAT

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Ca2+-dependent phosphorylation of syntaxin-1A by the death-associatedprotein (DAP) kinase regulates its interaction with Munc18."; Tian J.H., Das S., Sheng Z.H.; J. Biol. Chem. 278:26265-26274(2003). Cited for: PHOSPHORYLATION AT SER-188, AND INTERACTION WITH DAPK1 AND STKB1.	12730201 [Abstract]

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