HGS_RAT - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: HGS_RAT
• UniProt AC: Q9JJ50
• Protein Name: Hepatocyte growth factor-regulated tyrosine kinase substrate
• Gene Name: Hgs
• Organism: Rattus norvegicus (Rat).
• Sequence Length: 776
• Subcellular Localization: Cytoplasm . Early endosome membrane ; Peripheral membrane protein ; Cytoplasmic side . Endosome, multivesicular body membrane ; Peripheral membrane protein . Colocalizes with UBQLN1 in ubiquitin-rich cytoplasmic aggregates that are not endocytic comp
• Protein Description: Involved in intracellular signal transduction mediated by cytokines and growth factors. When associated with STAM, it suppresses DNA signaling upon stimulation by IL-2 and GM-CSF. Could be a direct effector of PI3-kinase in vesicular pathway via early endosomes and may regulate trafficking to early and late endosomes by recruiting clathrin. May concentrate ubiquitinated receptors within clathrin-coated regions. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with STAM (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes. Involved in receptor recycling via its association with the CART complex, a multiprotein complex required for efficient transferrin receptor recycling but not for EGFR degradation (By similarity). May contribute to the efficient recruitment of SMADs to the activin receptor complex..
• Protein Sequence: MGRGSGTFERLLDKATSQLLLETDWESILQICDLIRQGDTQAKYAVNSIKKKVNDKNPHVALYALEVMESVVKNCGQTVHDEVANKQTMEELKELLKRQVEVNVRNKILYLIQAWAHAFRNEPKYKVVQDTYQIMKVEGHVFPEFKESDAMFAAERAPDWVDAEECHRCRVQFGVVTRKHHCRACGQIFCGKCSSKYSTIPKFGIEKEVRVCEPCYEQLNKKAEGKAASTTELPPEYLTSPLSQQSQLPPKRDETALQEEEELQLALALSQSEAEEKERMRQKSTYTAHPKSEPAPLASSAPPAGSLYSSPVNSSAPLAEDIDPELARYLNRNYWEKKQEEARKSPTPSAPVPLTEPAAQPGEGHTAPNSMVEAPLPETDSQPITSCSGPFSEQYQNGESEESHEQFLKALQNAVSTFVNRMKSNHMRGRSITNDSAVLSLFQSINSTHPQLLELLNRLDERRLYYEGLQDKLAQIRDARGALSALREEHREKLRRAAEEAERQRQIQLAQKLEIMRQKKQEYLEVQRQLAIQRLQEQEKERQMRLEQQKQTVQMRAQMPAFPLPYAQLQAMPTAGGVLYQPSGPTSFPGTFSPAGSVEGSPMHGVYMSQPAPATGPYPSMPGTTADPSMVSAYMYPAGAPGAQAAPQAQAGPTTNPAYSSYQPTPTPGYQNVASQAPQSLPAISQPPQTSNIGYMGSQPMSMGYQPYNMQNLMTTLPGQDASLPAQQPYITGQQPMYQQMAPSTGPPQQQPPVAQPPPTQGPPAQGNETQLISFD

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
126	Ubiquitination	FRNEPKYKVVQDTYQ HHCCCCCCEEECHHH	41.89	-
132	Phosphorylation	YKVVQDTYQIMKVEG CCEEECHHHHHEECC	12.34	-
207	Acetylation	IPKFGIEKEVRVCEP CCCCCCCCEEEECHH	60.64	-
207	Ubiquitination	IPKFGIEKEVRVCEP CCCCCCCCEEEECHH	60.64	-
216	Phosphorylation	VRVCEPCYEQLNKKA EEECHHHHHHHHHHH	19.88	28689409
221	Ubiquitination	PCYEQLNKKAEGKAA HHHHHHHHHHCCCCC	63.05	-
222	Ubiquitination	CYEQLNKKAEGKAAS HHHHHHHHHCCCCCC	51.07	-
226	Ubiquitination	LNKKAEGKAASTTEL HHHHHCCCCCCCCCC	32.08	-
230	Phosphorylation	AEGKAASTTELPPEY HCCCCCCCCCCCHHH	21.40	25403869
231	Phosphorylation	EGKAASTTELPPEYL CCCCCCCCCCCHHHH	32.79	25403869
237	Phosphorylation	TTELPPEYLTSPLSQ CCCCCHHHHCCCHHH	22.51	25403869
239	Phosphorylation	ELPPEYLTSPLSQQS CCCHHHHCCCHHHCC	27.65	25403869
240	Phosphorylation	LPPEYLTSPLSQQSQ CCHHHHCCCHHHCCC	22.65	25403869
243	Phosphorylation	EYLTSPLSQQSQLPP HHHCCCHHHCCCCCC	29.56	25403869
246	Phosphorylation	TSPLSQQSQLPPKRD CCCHHHCCCCCCCCC	26.50	25403869
308	Phosphorylation	APPAGSLYSSPVNSS CCCCCCCCCCCCCCC	14.59	-
329	Phosphorylation	IDPELARYLNRNYWE CCHHHHHHHHHHHHH	11.61	-
334	Phosphorylation	ARYLNRNYWEKKQEE HHHHHHHHHHHHHHH	16.23	-
349	Phosphorylation	ARKSPTPSAPVPLTE HHCCCCCCCCCCCCC	47.91	22668510
550	Succinylation	QMRLEQQKQTVQMRA HHHHHHHHHHHHHHH	47.86	-
550	Succinylation	QMRLEQQKQTVQMRA HHHHHHHHHHHHHHH	47.86	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of HGS_RAT !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of HGS_RAT !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of HGS_RAT !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
TRAK2_RAT	Trak2	physical	17062640
NTRK2_RAT	Ntrk2	physical	19351881

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.