SNP25_RAT - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: SNP25_RAT
• UniProt AC: P60881
• Protein Name: Synaptosomal-associated protein 25
• Gene Name: Snap25
• Organism: Rattus norvegicus (Rat).
• Sequence Length: 206
• Subcellular Localization: Cytoplasm, perinuclear region . Cell membrane ; Lipid-anchor . Cell junction, synapse, synaptosome . Membrane association requires palmitoylation (By similarity). Expressed throughout cytoplasm, concentrating at the perinuclear region (By similarity)
• Protein Description: t-SNARE involved in the molecular regulation of neurotransmitter release. Modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1 in pancreatic beta cells. [PubMed: 12403834 May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF.]
• Protein Sequence: MAEDADMRNELEEMQRRADQLADESLESTRRMLQLVEESKDAGIRTLVMLDEQGEQLERIEEGMDQINKDMKEAEKNLTDLGKFCGLCVCPCNKLKSSDAYKKAWGNNQDGVVASQPARVVDEREQMAISGGFIRRVTNDARENEMDENLEQVSGIIGNLRHMALDMGNEIDTQNRQIDRIMEKADSNKTRIDEANQRATKMLGSG

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
25	Phosphorylation	ADQLADESLESTRRM HHHHHHHHHHHHHHH	37.67	28432305
28	Phosphorylation	LADESLESTRRMLQL HHHHHHHHHHHHHHH	32.29	28432305
29	Phosphorylation	ADESLESTRRMLQLV HHHHHHHHHHHHHHH	16.96	-
40	Ubiquitination	LQLVEESKDAGIRTL HHHHHHHHHCCCEEE	55.68	-
69	Ubiquitination	EGMDQINKDMKEAEK HHHHHHHHHHHHHHH	61.91	-
72	Ubiquitination	DQINKDMKEAEKNLT HHHHHHHHHHHHHHH	65.00	-
76	Ubiquitination	KDMKEAEKNLTDLGK HHHHHHHHHHHHHHH	65.39	-
85	S-palmitoylation	LTDLGKFCGLCVCPC HHHHHHHCCEEEECC	4.66	12837618
88	S-palmitoylation	LGKFCGLCVCPCNKL HHHHCCEEEECCCCC	1.47	12837618
90	S-palmitoylation	KFCGLCVCPCNKLKS HHCCEEEECCCCCCC	2.87	12837618
92	S-palmitoylation	CGLCVCPCNKLKSSD CCEEEECCCCCCCCH	6.24	12837618
102	Ubiquitination	LKSSDAYKKAWGNNQ CCCCHHHHHHHCCCC	37.42	-
103	Ubiquitination	KSSDAYKKAWGNNQD CCCHHHHHHHCCCCC	36.37	-
130	Phosphorylation	EREQMAISGGFIRRV HHHHHHHHCCHHHHH	24.27	28432305
138	Phosphorylation	GGFIRRVTNDARENE CCHHHHHCCCHHHHH	26.10	25403869
154	Phosphorylation	DENLEQVSGIIGNLR CCCHHHHHHHHHHHH	24.78	28551015
184	Ubiquitination	QIDRIMEKADSNKTR HHHHHHHHHHHCCCH	39.86	-
187	Phosphorylation	RIMEKADSNKTRIDE HHHHHHHHCCCHHHH	45.12	10965039
189	Ubiquitination	MEKADSNKTRIDEAN HHHHHHCCCHHHHHH	43.03	-
205	Phosphorylation	RATKMLGSG------ HHHHHHCCC------	37.05	28551015

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
138	T	Phosphorylation	Kinase	PKACA	P00517	PSP
138	T	Phosphorylation	Kinase	PRKACA	P27791	GPS
138	T	Phosphorylation	Kinase	PRKCA	P05696	GPS
138	T	Phosphorylation	Kinase	PKA-FAMILY	-	GPS
138	T	Phosphorylation	Kinase	PKC-FAMILY	-	GPS
138	T	Phosphorylation	Kinase	PKC	-	Uniprot
138	T	Phosphorylation	Kinase	PKA	-	Uniprot
187	S	Phosphorylation	Kinase	PRKCA	P17252	GPS
187	S	Phosphorylation	Kinase	PKCA	P05696	PSP
187	S	Phosphorylation	Kinase	PRKCB	P05771	GPS
187	S	Phosphorylation	Kinase	PKC-FAMILY	-	GPS
187	S	Phosphorylation	Kinase	PKC	-	Uniprot

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
85	C	Palmitoylation	Cys-85 appears to be the main site, and palmitoylation is required for membrane association (By similarity).	-

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of SNP25_RAT !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
STX1A_HUMAN	STX1A	physical	9168999
STX2_HUMAN	STX2	physical	9168999
STX3_HUMAN	STX3	physical	9168999
STX4_HUMAN	STX4	physical	9168999
HGS_RAT	Hgs	physical	10510169
HGS_RAT	Hgs	physical	10825299
STX1B_RAT	Stx1b	physical	10825299

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"Differential phosphorylation of SNAP-25 in vivo by protein kinase Cand protein kinase A.";
Hepp R., Cabaniols J.-P., Roche P.A.;
FEBS Lett. 532:52-56(2002).
Cited for: PHOSPHORYLATION AT THR-138 AND SER-187.
PubMed: 12459461