SPI1_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: SPI1_MOUSE
• UniProt AC: P17433
• Protein Name: Transcription factor PU.1
• Gene Name: Spi1
• Organism: Mus musculus (Mouse).
• Sequence Length: 272
• Subcellular Localization: Nucleus.
• Protein Description: Binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B-cells. Also binds RNA and may modulate pre-mRNA splicing..
• Protein Sequence: MLQACKMEGFSLTAPPSDDLVTYDSELYQRPMHDYYSFVGSDGESHSDHYWDFSAHHVHNNEFENFPENHFTELQSVQPPQLQQLYRHMELEQMHVLDTPMVPPHTGLSHQVSYMPRMCFPYQTLSPAHQQSSDEEEGERQSPPLEVSDGEADGLEPGPGLLHGETGSKKKIRLYQFLLDLLRSGDMKDSIWWVDKDKGTFQFSSKHKEALAHRWGIQKGNRKKMTYQKMARALRNYGKTGEVKKVKKKLTYQFSGEVLGRGGLAERRLPPH

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
41	Phosphorylation	DYYSFVGSDGESHSD CCCCEECCCCCCCCC	36.82	20190819
122	Phosphorylation	MPRMCFPYQTLSPAH CCCCCCCCCCCCHHH	8.22	25159016
124	Phosphorylation	RMCFPYQTLSPAHQQ CCCCCCCCCCHHHHC	24.30	25159016
126	Phosphorylation	CFPYQTLSPAHQQSS CCCCCCCCHHHHCCC	24.35	25159016
132	Phosphorylation	LSPAHQQSSDEEEGE CCHHHHCCCCCCCCC	32.94	17200120
133	Phosphorylation	SPAHQQSSDEEEGER CHHHHCCCCCCCCCC	44.71	17200120
142	Phosphorylation	EEEGERQSPPLEVSD CCCCCCCCCCEECCC	34.40	27180971
148	Phosphorylation	QSPPLEVSDGEADGL CCCCEECCCCCCCCC	30.44	22802335
227	Phosphorylation	GNRKKMTYQKMARAL CCHHHCHHHHHHHHH	11.57	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
142	S	Phosphorylation	Kinase	P38-SUBFAMILY	-	GPS
148	S	Phosphorylation	Kinase	CSNK2A1	Q60737	GPS

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of SPI1_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of SPI1_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
MTG16_MOUSE	Cbfa2t3	physical	21518930
SIN3A_MOUSE	Sin3a	physical	21518930
HDAC2_MOUSE	Hdac2	physical	21518930
PML_MOUSE	Pml	physical	17562868
EP300_MOUSE	Ep300	physical	17562868
IRF4_MOUSE	Irf4	physical	11133986
HXA10_MOUSE	Hoxa10	physical	18692240
JUN_MOUSE	Jun	physical	19181746
PRDM1_MOUSE	Prdm1	physical	17977828
CBP_HUMAN	CREBBP	physical	10050886

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"The phagosomal proteome in interferon-gamma-activated macrophages.";
Trost M., English L., Lemieux S., Courcelles M., Desjardins M.,Thibault P.;
Immunity 30:143-154(2009).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-148, AND MASSSPECTROMETRY.
PubMed: 19144319