SKP2_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: SKP2_MOUSE
• UniProt AC: Q9Z0Z3
• Protein Name: S-phase kinase-associated protein 2
• Gene Name: Skp2
• Organism: Mus musculus (Mouse).
• Sequence Length: 424
• Subcellular Localization: Cytoplasm. Nucleus.
• Protein Description: Substrate recognition component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. The SCF complex provides substrate specificity and interacts with both, the E2 ubiquitin-conjugating enzyme and the substrate. Specifically recognizes phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. Degradation of CDKN1B/p27kip also requires CKS1. Promotes ubiquitination and destruction of CDH1 in a CK1-Dependent Manner, thereby regulating cell migration (By similarity)..
• Protein Sequence: MHRKHLQEIPDQSGNVTTSFTWGWDSSKTSELLSGMGVSALEKEEVDSENIPHGLLSNLGHPQSPPRKRVKGKGSDKDFVIIRRPKLSRENFPGVSWDSLPDELLLGIFSCLCLPELLRVSGVCKRWYRLSLDESLWQSLDLAGKNLHPDVTVRLLSRGVVAFRCPRSFMEQPLGESFSSFRVQHMDLSNSVINVSNLHKILSECSKLQNLSLEGLQLSDPIVKTLAQNENLVRLNLCGCSGFSESAVATLLSSCSRLDELNLSWCFDFTEKHVQAAVAHLPNTITQLNLSGYRKNLQKTDLCTIIKRCPNLIRLDLSDSIMLKNDCFPEFFQLNYLQHLSLSRCYDIIPDTLLELGEIPTLKTLQVFGIVPEGTLQLLREALPRLQINCAYFTTIARPTMDSKKNLEIWGIKCRLTLQKPSCL

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
29	Phosphorylation	WGWDSSKTSELLSGM ECCCCCHHHHHHCCC	29.40	25777480
30	Phosphorylation	GWDSSKTSELLSGMG CCCCCHHHHHHCCCC	29.95	25777480
34	Phosphorylation	SKTSELLSGMGVSAL CHHHHHHCCCCCCHH	39.08	25777480
39	Phosphorylation	LLSGMGVSALEKEEV HHCCCCCCHHHHHHC	22.77	25777480
48	Phosphorylation	LEKEEVDSENIPHGL HHHHHCCCCCCCCHH	37.41	23984901
57	Phosphorylation	NIPHGLLSNLGHPQS CCCCHHHHHCCCCCC	34.74	21149613
64	Phosphorylation	SNLGHPQSPPRKRVK HHCCCCCCCCCCCCC	41.12	21149613
68	Acetylation	HPQSPPRKRVKGKGS CCCCCCCCCCCCCCC	68.28	51346005
71	Acetylation	SPPRKRVKGKGSDKD CCCCCCCCCCCCCCC	59.96	51346003
75	Phosphorylation	KRVKGKGSDKDFVII CCCCCCCCCCCEEEE	45.00	25338131
88	Phosphorylation	IIRRPKLSRENFPGV EEECCCCCCCCCCCC	43.61	28059163
179	Phosphorylation	QPLGESFSSFRVQHM CCCCCCCCCEEEEEC	37.53	-
224	Ubiquitination	QLSDPIVKTLAQNEN CCCCHHHHHHHCCCC	38.24	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
256	S	Phosphorylation	Kinase	AMPKB1	Q9Y478	PSP
256	S	Phosphorylation	Kinase	AMPKG2	Q9UGJ0	PSP
256	S	Phosphorylation	Kinase	AMPKA1	Q13131	PSP

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
68	K	Acetylation	Acetylation at Lys-68 and Lys-71 increases stability through impairment of APC/C-mediated proteolysis and promotes cytoplasmic retention.	-
71	K	Acetylation	Acetylation at Lys-68 and Lys-71 increases stability through impairment of APC/C-mediated proteolysis and promotes cytoplasmic retention.	-

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of SKP2_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
SRC_MOUSE	Src	physical	20596523
CDN1B_MOUSE	Cdkn1b	physical	11463388
CUL1_HUMAN	CUL1	physical	10790373
CUL1_MOUSE	Cul1	physical	11735228
SKP1_MOUSE	Skp1a	physical	11735228
E2F1_MOUSE	E2f1	physical	23868976
SKP1_MOUSE	Skp1a	physical	23868976
CUL1_MOUSE	Cul1	physical	23868976
CADH1_MOUSE	Cdh1	physical	24115035
SKP1_MOUSE	Skp1a	physical	24390425
CUL1_MOUSE	Cul1	physical	24390425
CUL1_HUMAN	CUL1	physical	24390425
HS90B_HUMAN	HSP90AB1	physical	24390425
CSN4_HUMAN	COPS4	physical	24390425
SKP1_HUMAN	SKP1	physical	24390425
CADH1_MOUSE	Cdh1	physical	17601983

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.