PTR7_ARATH - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: PTR7_ARATH
• UniProt AC: Q05085
• Protein Name: Protein NRT1/ PTR FAMILY 6.3
• Gene Name: NPF6.3
• Organism: Arabidopsis thaliana (Mouse-ear cress).
• Sequence Length: 590
• Subcellular Localization: Membrane ; Multi-pass membrane protein .
• Protein Description: Dual affinity nitrate transporter. Involved in proton-dependent nitrate uptake and in the regulation of the nitrate transporter NRT2.1. Acts also as a nitrate sensor that trigger a specific signaling pathway stimulating lateral root growth and seed germination. The uptake activity is not required for sensor function. Displays an auxin transport facilitation inhibited by high nitrate concentration. Required to prevent auxin accumulation in preemerged lateral root primordia and young lateral roots when external nitrate concentration is low or null. May be involved in the basipetal transport of auxin out of the lateral root tips. Acts as a bidirectional transporter involved in root-to-shoot nitrate translocation. Recognizes specifically nitrate and chlorate, but not nitrite, alanine, sulfate, phosphate or the di-peptide Ala-Ala..
• Protein Sequence: MSLPETKSDDILLDAWDFQGRPADRSKTGGWASAAMILCIEAVERLTTLGIGVNLVTYLTGTMHLGNATAANTVTNFLGTSFMLCLLGGFIADTFLGRYLTIAIFAAIQATGVSILTLSTIIPGLRPPRCNPTTSSHCEQASGIQLTVLYLALYLTALGTGGVKASVSGFGSDQFDETEPKERSKMTYFFNRFFFCINVGSLLAVTVLVYVQDDVGRKWGYGICAFAIVLALSVFLAGTNRYRFKKLIGSPMTQVAAVIVAAWRNRKLELPADPSYLYDVDDIIAAEGSMKGKQKLPHTEQFRSLDKAAIRDQEAGVTSNVFNKWTLSTLTDVEEVKQIVRMLPIWATCILFWTVHAQLTTLSVAQSETLDRSIGSFEIPPASMAVFYVGGLLLTTAVYDRVAIRLCKKLFNYPHGLRPLQRIGLGLFFGSMAMAVAALVELKRLRTAHAHGPTVKTLPLGFYLLIPQYLIVGIGEALIYTGQLDFFLRECPKGMKGMSTGLLLSTLALGFFFSSVLVTIVEKFTGKAHPWIADDLNKGRLYNFYWLVAVLVALNFLIFLVFSKWYVYKEKRLAEVGIELDDEPSIPMGH

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
101	Phosphorylation	TFLGRYLTIAIFAAI HHHHHHHHHHHHHHH	10.22	24572366
318	Phosphorylation	RDQEAGVTSNVFNKW HHCCCCCCCHHCCCC	17.42	30407730
319	Phosphorylation	DQEAGVTSNVFNKWT HCCCCCCCHHCCCCC	28.70	30407730

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
101	T	Phosphorylation	Kinase	CIPK23	Q93VD3	Uniprot

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of PTR7_ARATH !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of PTR7_ARATH !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
CIPKN_ARATH	CIPK23	physical	19766570
CDPK1_ARATH	CPK1	physical	22737156
HHP2_ARATH	HHP2	physical	24833385
UBC34_ARATH	UBC34	physical	24833385
WNK8_ARATH	WNK8	physical	24833385
C71BF_ARATH	PAD3	physical	24833385
LHWL3_ARATH	CPUORF7	physical	24833385
CNIH1_ARATH	AT3G12180	physical	24833385
POT2_ARATH	KT2	physical	24833385
CP21D_ARATH	AT3G66654	physical	24833385
BETL2_ARATH	AT1G29060	physical	24833385
BET12_ARATH	ATBET12	physical	24833385

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"Switching between the two action modes of the dual-affinity nitratetransporter CHL1 by phosphorylation.";
Liu K.H., Tsay Y.F.;
EMBO J. 22:1005-1013(2003).
Cited for: FUNCTION, MUTAGENESIS OF THR-28 AND THR-101, AND PHOSPHORYLATION ATTHR-101.
PubMed: 12606566