dbPTM

MAF1_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	MAF1_HUMAN
UniProt AC	Q9H063
Protein Name	Repressor of RNA polymerase III transcription MAF1 homolog
Gene Name	MAF1
Organism	Homo sapiens (Human).
Sequence Length	256
Subcellular Localization	Nucleus. Cytoplasm.
Protein Description	Element of the mTORC1 signaling pathway that acts as a mediator of diverse signals and that represses RNA polymerase III transcription. Inhibits the de novo assembly of TFIIIB onto DNA..
Protein Sequence	MKLLENSSFEAINSQLTVETGDAHIIGRIESYSCKMAGDDKHMFKQFCQEGQPHVLEALSPPQTSGLSPSRLSKSQGGEEEGPLSDKCSRKTLFYLIATLNESFRPDYDFSTARSHEFSREPSLSWVVNAVNCSLFSAVREDFKDLKPQLWNAVDEEICLAECDIYSYNPDLDSDPFGEDGSLWSFNYFFYNKRLKRIVFFSCRSISGSTYTPSEAGNELDMELGEEEVEEESRSGGSGAEETSTMEEDRVPVICI

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
35	Sumoylation	RIESYSCKMAGDDKH EEEEECCEECCCCHH	26.33	-
35	Sumoylation	RIESYSCKMAGDDKH EEEEECCEECCCCHH	26.33	-
60	Phosphorylation	PHVLEALSPPQTSGL CCHHHHCCCCCCCCC	41.40	30266825
64	Phosphorylation	EALSPPQTSGLSPSR HHCCCCCCCCCCHHH	30.45	23401153
65	Phosphorylation	ALSPPQTSGLSPSRL HCCCCCCCCCCHHHH	32.27	23401153
68	Phosphorylation	PPQTSGLSPSRLSKS CCCCCCCCHHHHCCC	25.10	22167270
70	Phosphorylation	QTSGLSPSRLSKSQG CCCCCCHHHHCCCCC	42.34	30266825
73	Phosphorylation	GLSPSRLSKSQGGEE CCCHHHHCCCCCCCC	29.20	30266825
74	Ubiquitination	LSPSRLSKSQGGEEE CCHHHHCCCCCCCCC	51.93	27667366
75	Phosphorylation	SPSRLSKSQGGEEEG CHHHHCCCCCCCCCC	30.59	29255136
85	Phosphorylation	GEEEGPLSDKCSRKT CCCCCCCCHHHCHHH	37.85	23403867
87	Ubiquitination	EEGPLSDKCSRKTLF CCCCCCHHHCHHHHH	30.63	-
89	Phosphorylation	GPLSDKCSRKTLFYL CCCCHHHCHHHHHHH	42.42	23312004
205	Phosphorylation	IVFFSCRSISGSTYT EEEEEEEECCCCCCC	25.98	29978859
207	Phosphorylation	FFSCRSISGSTYTPS EEEEEECCCCCCCCC	28.12	29978859
209	Phosphorylation	SCRSISGSTYTPSEA EEEECCCCCCCCCHH	16.08	29978859
210	Phosphorylation	CRSISGSTYTPSEAG EEECCCCCCCCCHHC	34.63	29978859
211	Phosphorylation	RSISGSTYTPSEAGN EECCCCCCCCCHHCC	20.53	29978859
212	Phosphorylation	SISGSTYTPSEAGNE ECCCCCCCCCHHCCC	22.04	29978859
214	Phosphorylation	SGSTYTPSEAGNELD CCCCCCCCHHCCCCC	32.07	11230166
235	Phosphorylation	EVEEESRSGGSGAEE HHHHHHHCCCCCCCC	57.70	24719451
238	Phosphorylation	EESRSGGSGAEETST HHHHCCCCCCCCCCC	38.05	28348404
243	Phosphorylation	GGSGAEETSTMEEDR CCCCCCCCCCCCCCC	22.47	28787133
244	Phosphorylation	GSGAEETSTMEEDRV CCCCCCCCCCCCCCC	29.09	28348404
245	Phosphorylation	SGAEETSTMEEDRVP CCCCCCCCCCCCCCC	35.70	28348404

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
60	S	Phosphorylation	Kinase	MTOR	P42345	Uniprot
68	S	Phosphorylation	Kinase	MTOR	P42345	Uniprot
75	S	Phosphorylation	Kinase	MTOR	P42345	Uniprot

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Reference
35	K	Sumoylation	23673667
Sumoylated with SUMO1 and SUMO2, mainly on Lys-35.
60	S	Phosphorylation	18669648
Phosphorylated at Ser-60, Ser-68 and Ser-75; the major sites of phosphorylation.
68	S	Phosphorylation	20233713
Phosphorylated at Ser-60, Ser-68 and Ser-75; the major sites of phosphorylation.
75	S	Phosphorylation	17081983
Phosphorylated at Ser-60, Ser-68 and Ser-75; the major sites of phosphorylation.

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of MAF1_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
MAF1_HUMAN	MAF1	physical	20817737
RAD50_HUMAN	RAD50	physical	26186194
TSR3_HUMAN	TSR3	physical	26186194
KC1D_HUMAN	CSNK1D	physical	26186194
VCIP1_HUMAN	VCPIP1	physical	26186194
ARMX3_HUMAN	ARMCX3	physical	26186194
RPC1_HUMAN	POLR3A	physical	26186194
RPC6_HUMAN	POLR3F	physical	26186194
RPC2_HUMAN	POLR3B	physical	26186194
RPC6_HUMAN	POLR3F	physical	28514442
VCIP1_HUMAN	VCPIP1	physical	28514442
TSR3_HUMAN	TSR3	physical	28514442
ARMX3_HUMAN	ARMCX3	physical	28514442
FSBP_HUMAN	RAD54B	physical	28514442
RA54B_HUMAN	RAD54B	physical	28514442
RAD50_HUMAN	RAD50	physical	28514442
KC1D_HUMAN	CSNK1D	physical	28514442
RPC1_HUMAN	POLR3A	physical	28514442

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
There are no disease associations of PTM sites.
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of MAF1_HUMAN

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"mTOR associates with TFIIIC, is found at tRNA and 5S rRNA genes, andtargets their repressor Maf1."; Kantidakis T., Ramsbottom B.A., Birch J.L., Dowding S.N., White R.J.; Proc. Natl. Acad. Sci. U.S.A. 107:11823-11828(2010). Cited for: FUNCTION, PHOSPHORYLATION AT SER-75, SUBCELLULAR LOCATION, ANDMUTAGENESIS OF SER-75.	20543138 [Abstract]
"mTORC1 directly phosphorylates and regulates human MAF1."; Michels A.A., Robitaille A.M., Buczynski-Ruchonnet D., Hodroj W.,Reina J.H., Hall M.N., Hernandez N.; Mol. Cell. Biol. 30:3749-3757(2010). Cited for: FUNCTION, PHOSPHORYLATION AT SER-60; SER-68 AND SER-75 BY MTOR,PHOSPHORYLATION AT THR-64; SER-65; SER-70; THR-212 AND SER-214, ANDMUTAGENESIS OF SER-60; SER-68 AND SER-75.	20516213 [Abstract]
"Requirement of the mTOR kinase for the regulation of Maf1phosphorylation and control of RNA polymerase III-dependenttranscription in cancer cells."; Shor B., Wu J., Shakey Q., Toral-Barza L., Shi C., Follettie M.,Yu K.; J. Biol. Chem. 285:15380-15392(2010). Cited for: FUNCTION, PHOSPHORYLATION AT SER-60; THR-64; SER-68 AND SER-75,SUBCELLULAR LOCATION, AND MUTAGENESIS OF SER-60; THR-64; SER-68 ANDSER-75.	20233713 [Abstract]
"Quantitative phosphoproteomic analysis of T cell receptor signalingreveals system-wide modulation of protein-protein interactions."; Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K.,Rodionov V., Han D.K.; Sci. Signal. 2:RA46-RA46(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-75, AND MASSSPECTROMETRY.	19690332 [Abstract]
"A quantitative atlas of mitotic phosphorylation."; Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,Elledge S.J., Gygi S.P.; Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-60; SER-68 AND SER-75,AND MASS SPECTROMETRY.	18669648 [Abstract]
"Global, in vivo, and site-specific phosphorylation dynamics insignaling networks."; Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P.,Mann M.; Cell 127:635-648(2006). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-75, AND MASSSPECTROMETRY.	17081983 [Abstract]

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