LEF1_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: LEF1_MOUSE
• UniProt AC: P27782
• Protein Name: Lymphoid enhancer-binding factor 1
• Gene Name: Lef1
• Organism: Mus musculus (Mouse).
• Sequence Length: 397
• Subcellular Localization: Nucleus. Found in nuclear bodies upon PIASG binding.
• Protein Description: Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1 (By similarity). Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIASG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1..
• Protein Sequence: MPQLSGGGGGGDPELCATDEMIPFKDEGDPQKEKIFAEISHPEEEGDLADIKSSLVNESEIIPASNGHEVVRQAPSSQEPYHDKAREHPDEGKHPDGGLYNKGPSYSSYSGYIMMPNMNSDPYMSNGSLSPPIPRTSNKVPVVQPSHAVHPLTPLITYSDEHFSPGSHPSHIPSDVNSKQGMSRHPPAPEIPTFYPLSPGGVGQITPPIGWQGQPVYPITGGFRQPYPSSLSGDTSMSRFSHHMIPGPPGPHTTGIPHPAIVTPQVKQEHPHTDSDLMHVKPQHEQRKEQEPKRPHIKKPLNAFMLYMKEMRANVVAECTLKESAAINQILGRRWHALSREEQAKYYELARKERQLHMQLYPGWSARDNYGKKKKRKREKLQESTSGTGPRMTAAYI

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
25	Sumoylation	TDEMIPFKDEGDPQK CCCEECCCCCCCHHH	49.90	-
25	Sumoylation	TDEMIPFKDEGDPQK CCCEECCCCCCCHHH	49.90	-
40	Phosphorylation	EKIFAEISHPEEEGD HEEEEECCCCHHCCC	25.21	27600695
77	Phosphorylation	VVRQAPSSQEPYHDK HEECCCCCCCCCCHH	37.19	28285833
130	Phosphorylation	YMSNGSLSPPIPRTS CCCCCCCCCCCCCCC	30.26	-
146	Phosphorylation	KVPVVQPSHAVHPLT CCCCCCCHHCCCCCC	13.64	25367039
153	Phosphorylation	SHAVHPLTPLITYSD HHCCCCCCCCEEECC	22.09	25367039
157	Phosphorylation	HPLTPLITYSDEHFS CCCCCCEEECCCCCC	25.34	25367039
158	Phosphorylation	PLTPLITYSDEHFSP CCCCCEEECCCCCCC	13.71	25367039
159	Phosphorylation	LTPLITYSDEHFSPG CCCCEEECCCCCCCC	28.07	25367039
164	Phosphorylation	TYSDEHFSPGSHPSH EECCCCCCCCCCCCC	30.20	25367039
167	Phosphorylation	DEHFSPGSHPSHIPS CCCCCCCCCCCCCCC	35.29	25367039
170	Phosphorylation	FSPGSHPSHIPSDVN CCCCCCCCCCCCCCC	28.73	25367039
174	Phosphorylation	SHPSHIPSDVNSKQG CCCCCCCCCCCCCCC	54.29	25367039
178	Phosphorylation	HIPSDVNSKQGMSRH CCCCCCCCCCCCCCC	26.63	25367039
183	Phosphorylation	VNSKQGMSRHPPAPE CCCCCCCCCCCCCCC	33.97	25367039
238	Phosphorylation	LSGDTSMSRFSHHMI CCCCCCCHHCCCCCC	29.91	-
267	Sumoylation	AIVTPQVKQEHPHTD CCCCCCCCCCCCCCC	44.73	-
267	Sumoylation	AIVTPQVKQEHPHTD CCCCCCCCCCCCCCC	44.73	-
339	Phosphorylation	GRRWHALSREEQAKY HHHHHHCCHHHHHHH	38.13	-
346	Phosphorylation	SREEQAKYYELARKE CHHHHHHHHHHHHHH	12.75	29895711
347	Phosphorylation	REEQAKYYELARKER HHHHHHHHHHHHHHH	12.08	-
385	Phosphorylation	REKLQESTSGTGPRM HHHHHHHCCCCCCCC	30.35	28285833

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
40	S	Phosphorylation	Kinase	CK2-FAMILY	-	GPS
153	T	Phosphorylation	Kinase	NLK	O54949	Uniprot
164	S	Phosphorylation	Kinase	NLK	O54949	Uniprot

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
153	T	Phosphorylation	Phosphorylated at Thr-153 and/or Ser-164 by NLK.	-
164	S	Phosphorylation	Phosphorylated at Thr-153 and/or Ser-164 by NLK.	-

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of LEF1_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
PIAS4_MOUSE	Pias4	physical	20211142
PAX3_MOUSE	Pax3	physical	15729346
TLE4_MOUSE	Tle4	physical	15729346
PAX3_MOUSE	Pax3	physical	20006729
HMGB2_MOUSE	Hmgb2	physical	19805379
SMAD4_MOUSE	Smad4	physical	17452626
SMAD2_MOUSE	Smad2	physical	17452626
CTNB1_MOUSE	Ctnnb1	physical	17445771
CTNB1_MOUSE	Ctnnb1	physical	16309666
PAX5_MOUSE	Pax5	physical	12244173
MYB_MOUSE	Myb	physical	12244173
TDG_MOUSE	Tdg	physical	24748645
TCF7_MOUSE	Tcf7	physical	24748645
TF7L2_MOUSE	Tcf7l2	physical	24748645
CTNB1_MOUSE	Ctnnb1	physical	24748645

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.