HXK1_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: HXK1_HUMAN
• UniProt AC: P19367
• Protein Name: Hexokinase-1
• Gene Name: HK1
• Organism: Homo sapiens (Human).
• Sequence Length: 917
• Subcellular Localization: Mitochondrion outer membrane. Its hydrophobic N-terminal sequence may be involved in membrane binding.
• Protein Sequence: MIAAQLLAYYFTELKDDQVKKIDKYLYAMRLSDETLIDIMTRFRKEMKNGLSRDFNPTATVKMLPTFVRSIPDGSEKGDFIALDLGGSSFRILRVQVNHEKNQNVHMESEVYDTPENIVHGSGSQLFDHVAECLGDFMEKRKIKDKKLPVGFTFSFPCQQSKIDEAILITWTKRFKASGVEGADVVKLLNKAIKKRGDYDANIVAVVNDTVGTMMTCGYDDQHCEVGLIIGTGTNACYMEELRHIDLVEGDEGRMCINTEWGAFGDDGSLEDIRTEFDREIDRGSLNPGKQLFEKMVSGMYLGELVRLILVKMAKEGLLFEGRITPELLTRGKFNTSDVSAIEKNKEGLHNAKEILTRLGVEPSDDDCVSVQHVCTIVSFRSANLVAATLGAILNRLRDNKGTPRLRTTVGVDGSLYKTHPQYSRRFHKTLRRLVPDSDVRFLLSESGSGKGAAMVTAVAYRLAEQHRQIEETLAHFHLTKDMLLEVKKRMRAEMELGLRKQTHNNAVVKMLPSFVRRTPDGTENGDFLALDLGGTNFRVLLVKIRSGKKRTVEMHNKIYAIPIEIMQGTGEELFDHIVSCISDFLDYMGIKGPRMPLGFTFSFPCQQTSLDAGILITWTKGFKATDCVGHDVVTLLRDAIKRREEFDLDVVAVVNDTVGTMMTCAYEEPTCEVGLIVGTGSNACYMEEMKNVEMVEGDQGQMCINMEWGAFGDNGCLDDIRTHYDRLVDEYSLNAGKQRYEKMISGMYLGEIVRNILIDFTKKGFLFRGQISETLKTRGIFETKFLSQIESDRLALLQVRAILQQLGLNSTCDDSILVKTVCGVVSRRAAQLCGAGMAAVVDKIRENRGLDRLNVTVGVDGTLYKLHPHFSRIMHQTVKELSPKCNVSFLLSEDGSGKGAALITAVGVRLRTEASS

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
1	Acetylation	-------MIAAQLLA -------CHHHHHHH	4.19	25944712
9	Phosphorylation	IAAQLLAYYFTELKD HHHHHHHHHHHHCCH	10.09	23663014
10	Phosphorylation	AAQLLAYYFTELKDD HHHHHHHHHHHCCHH	9.83	23663014
12	Phosphorylation	QLLAYYFTELKDDQV HHHHHHHHHCCHHHH	25.04	23663014
21	Ubiquitination	LKDDQVKKIDKYLYA CCHHHHHHHHHHHHH	58.23	21890473
21 (in isoform 1)	Ubiquitination	-	58.23	21890473
24	Malonylation	DQVKKIDKYLYAMRL HHHHHHHHHHHHHHC	40.40	26320211
25	Phosphorylation	QVKKIDKYLYAMRLS HHHHHHHHHHHHHCC	10.95	-
27	Phosphorylation	KKIDKYLYAMRLSDE HHHHHHHHHHHCCHH	8.68	-
32	Phosphorylation	YLYAMRLSDETLIDI HHHHHHCCHHHHHHH	24.04	20068231
35	Phosphorylation	AMRLSDETLIDIMTR HHHCCHHHHHHHHHH	33.72	20068231
41	Phosphorylation	ETLIDIMTRFRKEMK HHHHHHHHHHHHHHH	27.30	20068231
61 (in isoform 2)	Ubiquitination	-	2.96	-
62	Ubiquitination	FNPTATVKMLPTFVR CCCCCHHEECCEEHH	30.30	-
66	Phosphorylation	ATVKMLPTFVRSIPD CHHEECCEEHHCCCC	30.70	28270605
70	Phosphorylation	MLPTFVRSIPDGSEK ECCEEHHCCCCCCCC	32.92	28270605
75	Phosphorylation	VRSIPDGSEKGDFIA HHCCCCCCCCCCEEE	43.44	28270605
76 (in isoform 2)	Ubiquitination	-	67.59	-
77	Acetylation	SIPDGSEKGDFIALD CCCCCCCCCCEEEEE	66.53	23954790
88	Phosphorylation	IALDLGGSSFRILRV EEEECCCCCEEEEEE	25.22	25693802
89	Phosphorylation	ALDLGGSSFRILRVQ EEECCCCCEEEEEEE	23.09	24076635
92 (in isoform 3)	Phosphorylation	-	2.44	27251275
109	Phosphorylation	NQNVHMESEVYDTPE CCCEEECEEEEECCC	25.45	25693802
112	Phosphorylation	VHMESEVYDTPENIV EEECEEEEECCCCEE	15.28	25693802
114	Phosphorylation	MESEVYDTPENIVHG ECEEEEECCCCEECC	18.15	25693802
122	Phosphorylation	PENIVHGSGSQLFDH CCCEECCCHHHHHHH	22.01	25693802
124	Phosphorylation	NIVHGSGSQLFDHVA CEECCCHHHHHHHHH	25.96	25693802
147	Ubiquitination	KRKIKDKKLPVGFTF HCCCCCCCCCCEEEE	69.06	-
153	Phosphorylation	KKLPVGFTFSFPCQQ CCCCCEEEEEEECCC	16.67	29514088
155	Phosphorylation	LPVGFTFSFPCQQSK CCCEEEEEEECCCCC	25.81	29514088
173	Ubiquitination	AILITWTKRFKASGV EEEEEEHHCCHHCCC	47.61	-
175 (in isoform 2)	Ubiquitination	-	9.15	-
176	Malonylation	ITWTKRFKASGVEGA EEEHHCCHHCCCCCH	45.75	32601280
176	Methylation	ITWTKRFKASGVEGA EEEHHCCHHCCCCCH	45.75	73914825
176	Ubiquitination	ITWTKRFKASGVEGA EEEHHCCHHCCCCCH	45.75	-
178	Phosphorylation	WTKRFKASGVEGADV EHHCCHHCCCCCHHH	43.92	24719451
182 (in isoform 3)	Phosphorylation	-	25.35	24719451
186 (in isoform 2)	Ubiquitination	-	3.58	-
187	Acetylation	VEGADVVKLLNKAIK CCCHHHHHHHHHHHH	47.29	25038526
187	Ubiquitination	VEGADVVKLLNKAIK CCCHHHHHHHHHHHH	47.29	21890473
187 (in isoform 1)	Ubiquitination	-	47.29	21890473
190 (in isoform 2)	Ubiquitination	-	41.99	-
191	Malonylation	DVVKLLNKAIKKRGD HHHHHHHHHHHHCCC	52.00	26320211
191	Ubiquitination	DVVKLLNKAIKKRGD HHHHHHHHHHHHCCC	52.00	21890473
191 (in isoform 1)	Ubiquitination	-	52.00	21890473
269	Phosphorylation	GAFGDDGSLEDIRTE CCCCCCCCHHHHHHH	35.26	21815630
273 (in isoform 3)	Phosphorylation	-	4.24	24719451
289 (in isoform 2)	Ubiquitination	-	29.30	-
290	Ubiquitination	RGSLNPGKQLFEKMV CCCCCCCHHHHHHHH	45.12	-
298	Phosphorylation	QLFEKMVSGMYLGEL HHHHHHHHCCHHHHH	17.84	20068231
300	Sulfoxidation	FEKMVSGMYLGELVR HHHHHHCCHHHHHHH	1.62	28465586
301	Phosphorylation	EKMVSGMYLGELVRL HHHHHCCHHHHHHHH	18.32	20068231
311 (in isoform 2)	Ubiquitination	-	2.94	-
312	2-Hydroxyisobutyrylation	LVRLILVKMAKEGLL HHHHHHHHHHHCCCC	29.69	-
315	2-Hydroxyisobutyrylation	LILVKMAKEGLLFEG HHHHHHHHCCCCCCC	49.14	-
315	Ubiquitination	LILVKMAKEGLLFEG HHHHHHHHCCCCCCC	49.14	-
332 (in isoform 2)	Ubiquitination	-	33.40	-
333	2-Hydroxyisobutyrylation	PELLTRGKFNTSDVS HHHHHCCCCCHHHCH	31.95	-
333	Acetylation	PELLTRGKFNTSDVS HHHHHCCCCCHHHCH	31.95	25953088
333	Malonylation	PELLTRGKFNTSDVS HHHHHCCCCCHHHCH	31.95	26320211
333	Ubiquitination	PELLTRGKFNTSDVS HHHHHCCCCCHHHCH	31.95	21890473
333 (in isoform 1)	Ubiquitination	-	31.95	21890473
336	Phosphorylation	LTRGKFNTSDVSAIE HHCCCCCHHHCHHHH	29.45	25850435
337	Phosphorylation	TRGKFNTSDVSAIEK HCCCCCHHHCHHHHH	36.80	25850435
340	Phosphorylation	KFNTSDVSAIEKNKE CCCHHHCHHHHHCCC	28.64	25850435
340 (in isoform 3)	Phosphorylation	-	28.64	24719451
343 (in isoform 2)	Ubiquitination	-	50.74	-
344	Malonylation	SDVSAIEKNKEGLHN HHCHHHHHCCCCHHC	68.18	26320211
344	Ubiquitination	SDVSAIEKNKEGLHN HHCHHHHHCCCCHHC	68.18	-
345 (in isoform 2)	Ubiquitination	-	34.52	-
346	2-Hydroxyisobutyrylation	VSAIEKNKEGLHNAK CHHHHHCCCCHHCHH	65.31	-
346	Ubiquitination	VSAIEKNKEGLHNAK CHHHHHCCCCHHCHH	65.31	-
364	Phosphorylation	TRLGVEPSDDDCVSV HHHCCCCCCCCCCCH	39.92	-
403	Phosphorylation	RLRDNKGTPRLRTTV HHHCCCCCCCCEEEE	13.57	24719451
407 (in isoform 3)	Phosphorylation	-	29.63	24719451
408	Phosphorylation	KGTPRLRTTVGVDGS CCCCCCEEEECCCCC	30.35	29759185
409	Phosphorylation	GTPRLRTTVGVDGSL CCCCCEEEECCCCCC	14.61	29759185
415	Phosphorylation	TTVGVDGSLYKTHPQ EEECCCCCCEECCHH	24.86	29759185
417	Phosphorylation	VGVDGSLYKTHPQYS ECCCCCCEECCHHHH	18.61	29083192
418	2-Hydroxyisobutyrylation	GVDGSLYKTHPQYSR CCCCCCEECCHHHHH	45.72	-
418	Malonylation	GVDGSLYKTHPQYSR CCCCCCEECCHHHHH	45.72	26320211
418	Ubiquitination	GVDGSLYKTHPQYSR CCCCCCEECCHHHHH	45.72	21890473
418 (in isoform 1)	Ubiquitination	-	45.72	21890473
419	Phosphorylation	VDGSLYKTHPQYSRR CCCCCEECCHHHHHH	25.82	-
429	Acetylation	QYSRRFHKTLRRLVP HHHHHHHHHHHHHCC	46.68	25953088
445	Phosphorylation	SDVRFLLSESGSGKG HHHEEEEECCCCCHH	31.68	22673903
447	Phosphorylation	VRFLLSESGSGKGAA HEEEEECCCCCHHHH	34.58	23312004
449	Phosphorylation	FLLSESGSGKGAAMV EEEECCCCCHHHHHH	46.76	22673903
457	Phosphorylation	GKGAAMVTAVAYRLA CHHHHHHHHHHHHHH	11.43	-
461	Phosphorylation	AMVTAVAYRLAEQHR HHHHHHHHHHHHHHH	10.28	24927040
488	2-Hydroxyisobutyrylation	KDMLLEVKKRMRAEM HHHHHHHHHHHHHHH	25.86	-
489	Acetylation	DMLLEVKKRMRAEME HHHHHHHHHHHHHHH	57.51	22424773
495	Sulfoxidation	KKRMRAEMELGLRKQ HHHHHHHHHHCCCCC	5.13	30846556
501	Ubiquitination	EMELGLRKQTHNNAV HHHHCCCCCCCCHHH	65.67	-
503	Phosphorylation	ELGLRKQTHNNAVVK HHCCCCCCCCHHHHH	29.61	-
510	Ubiquitination	THNNAVVKMLPSFVR CCCHHHHHCHHHHHH	28.28	-
519	Phosphorylation	LPSFVRRTPDGTENG HHHHHHCCCCCCCCC	18.37	24076635
523	Phosphorylation	VRRTPDGTENGDFLA HHCCCCCCCCCCEEE	33.39	24076635
544	Acetylation	NFRVLLVKIRSGKKR EEEEEEEEECCCCCE	32.16	30585873
624	Malonylation	ITWTKGFKATDCVGH EEECCCCCCCCCCCH	60.07	26320211
624	Ubiquitination	ITWTKGFKATDCVGH EEECCCCCCCCCCCH	60.07	-
727	Methylation	DIRTHYDRLVDEYSL HHHHHHHHHHHHHCC	29.46	115478743
732	Phosphorylation	YDRLVDEYSLNAGKQ HHHHHHHHCCHHCHH	17.62	-
733	Phosphorylation	DRLVDEYSLNAGKQR HHHHHHHCCHHCHHH	17.07	-
737 (in isoform 2)	Ubiquitination	-	26.09	-
738	Ubiquitination	EYSLNAGKQRYEKMI HHCCHHCHHHHHHHH	29.23	-
746	Phosphorylation	QRYEKMISGMYLGEI HHHHHHHCHHHHHHH	17.63	-
749	Phosphorylation	EKMISGMYLGEIVRN HHHHCHHHHHHHHHH	18.32	-
762 (in isoform 2)	Ubiquitination	-	28.26	-
763	2-Hydroxyisobutyrylation	NILIDFTKKGFLFRG HHHHHHHCCCEEECC	51.39	-
763	Acetylation	NILIDFTKKGFLFRG HHHHHHHCCCEEECC	51.39	22424773
763	Malonylation	NILIDFTKKGFLFRG HHHHHHHCCCEEECC	51.39	26320211
763	Ubiquitination	NILIDFTKKGFLFRG HHHHHHHCCCEEECC	51.39	-
763 (in isoform 1)	Ubiquitination	-	51.39	21890473
776 (in isoform 2)	Ubiquitination	-	4.72	-
777	Acetylation	GQISETLKTRGIFET CCCCHHHHHCCCCCH	43.53	25953088
777	Malonylation	GQISETLKTRGIFET CCCCHHHHHCCCCCH	43.53	26320211
777	Ubiquitination	GQISETLKTRGIFET CCCCHHHHHCCCCCH	43.53	-
784 (in isoform 2)	Ubiquitination	-	19.15	-
788	Phosphorylation	IFETKFLSQIESDRL CCCHHHHHHHHHHHH	32.04	28857561
792	Phosphorylation	KFLSQIESDRLALLQ HHHHHHHHHHHHHHH	29.65	24076635
813	S-nitrosocysteine	QLGLNSTCDDSILVK HCCCCCCCCCHHHHH	5.70	-
813	S-nitrosylation	QLGLNSTCDDSILVK HCCCCCCCCCHHHHH	5.70	19483679
821	O-linked_Glycosylation	DDSILVKTVCGVVSR CCHHHHHHHHHHHHH	17.35	30379171
821	Phosphorylation	DDSILVKTVCGVVSR CCHHHHHHHHHHHHH	17.35	21406692
827	Phosphorylation	KTVCGVVSRRAAQLC HHHHHHHHHHHHHHH	17.40	21406692
844	Acetylation	GMAAVVDKIRENRGL CHHHHHHHHHHCCCC	31.86	25038526
849	Methylation	VDKIRENRGLDRLNV HHHHHHCCCCCCEEE	42.15	115478735
872	Phosphorylation	YKLHPHFSRIMHQTV EECCHHHHHHHHHHH	20.19	22468782
875	Sulfoxidation	HPHFSRIMHQTVKEL CHHHHHHHHHHHHHH	1.54	30846556
878	Phosphorylation	FSRIMHQTVKELSPK HHHHHHHHHHHHCCC	20.90	22468782
879 (in isoform 2)	Ubiquitination	-	3.50	-
880	Acetylation	RIMHQTVKELSPKCN HHHHHHHHHHCCCCC	57.22	25953088
880	Malonylation	RIMHQTVKELSPKCN HHHHHHHHHHCCCCC	57.22	26320211
880	Ubiquitination	RIMHQTVKELSPKCN HHHHHHHHHHCCCCC	57.22	-
885	Malonylation	TVKELSPKCNVSFLL HHHHHCCCCCEEEEE	34.76	26320211
885	Ubiquitination	TVKELSPKCNVSFLL HHHHHCCCCCEEEEE	34.76	-
889	Phosphorylation	LSPKCNVSFLLSEDG HCCCCCEEEEECCCC	9.00	30622161
893	Phosphorylation	CNVSFLLSEDGSGKG CCEEEEECCCCCCCC	35.47	-
913	Phosphorylation	AVGVRLRTEASS--- EEEEEEECCCCC---	40.26	-
916	Phosphorylation	VRLRTEASS------ EEEECCCCC------	30.28	29514088
917	Phosphorylation	RLRTEASS------- EEECCCCC-------	51.82	29514088
921 (in isoform 3)	Phosphorylation	-		24719451

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
124	S	Phosphorylation	Kinase	ULK1	O75385	PSP
364	S	Phosphorylation	Kinase	ULK1	O75385	PSP
732	Y	Phosphorylation	Kinase	SRC	P12931	PSP
-	K	Ubiquitination	E3 ubiquitin ligase	PRKN	O60260	PMID:23068103

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of HXK1_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of HXK1_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
A4_HUMAN	APP	physical	21832049
ATF2_HUMAN	ATF2	physical	22304920
VDAC1_HUMAN	VDAC1	physical	22304920
SAHH_HUMAN	AHCY	physical	22863883
ARLY_HUMAN	ASL	physical	22863883
CAN2_HUMAN	CAPN2	physical	22863883
TF3C4_HUMAN	GTF3C4	physical	22863883
NSF1C_HUMAN	NSFL1C	physical	22863883
PARP6_HUMAN	PARP6	physical	22863883
PCNA_HUMAN	PCNA	physical	22863883
PLAP_HUMAN	PLAA	physical	22863883
TBB2A_HUMAN	TUBB2A	physical	22863883
EFTU_HUMAN	TUFM	physical	22863883
TWF2_HUMAN	TWF2	physical	22863883
UBA5_HUMAN	UBA5	physical	22863883
UBXN1_HUMAN	UBXN1	physical	22863883
ASPD_HUMAN	ASPDH	physical	26344197
HKDC1_HUMAN	HKDC1	physical	28514442
HXK2_HUMAN	HK2	physical	28514442
CAV1_HUMAN	CAV1	physical	28514442
SYUA_HUMAN	SNCA	physical	28514442
ASPC1_HUMAN	ASPSCR1	physical	28514442

Drug and Disease Associations

• Kegg Disease
• H00664: Anemia due to disorders of glycolytic enzymes, including: Hexokinase (HK) deficiency; Phosphoglycera
• OMIM Disease
• 235700: Hexokinase deficiency (HK deficiency)
• 605285: Hereditary motor and sensory neuropathy, Russe type (HMSNR)
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.