HMGCL_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: HMGCL_HUMAN
• UniProt AC: P35914
• Protein Name: Hydroxymethylglutaryl-CoA lyase, mitochondrial
• Gene Name: HMGCL
• Organism: Homo sapiens (Human).
• Sequence Length: 325
• Subcellular Localization: Mitochondrion matrix. Peroxisome. Unprocessed form is peroxisomal.
• Protein Description: Key enzyme in ketogenesis (ketone body formation). Terminal step in leucine catabolism. Ketone bodies (beta-hydroxybutyrate, acetoacetate and acetone) are essential as an alternative source of energy to glucose, as lipid precursors and as regulators of metabolism..
• Protein Sequence: MAAMRKALPRRLVGLASLRAVSTSSMGTLPKRVKIVEVGPRDGLQNEKNIVSTPVKIKLIDMLSEAGLSVIETTSFVSPKWVPQMGDHTEVLKGIQKFPGINYPVLTPNLKGFEAAVAAGAKEVVIFGAASELFTKKNINCSIEESFQRFDAILKAAQSANISVRGYVSCALGCPYEGKISPAKVAEVTKKFYSMGCYEISLGDTIGVGTPGIMKDMLSAVMQEVPLAALAVHCHDTYGQALANTLMALQMGVSVVDSSVAGLGGCPYAQGASGNLATEDLVYMLEGLGIHTGVNLQKLLEAGNFICQALNRKTSSKVAQATCKL

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
17	Phosphorylation	RRLVGLASLRAVSTS HHHHHHHHHHHCCCC	24.35	23186163
22	Phosphorylation	LASLRAVSTSSMGTL HHHHHHCCCCCCCCC	22.91	26434776
23	Phosphorylation	ASLRAVSTSSMGTLP HHHHHCCCCCCCCCC	20.44	26434776
48	Succinylation	RDGLQNEKNIVSTPV CCCCCCCCCCCCCCC	60.57	23954790
48	Malonylation	RDGLQNEKNIVSTPV CCCCCCCCCCCCCCC	60.57	26320211
48	Ubiquitination	RDGLQNEKNIVSTPV CCCCCCCCCCCCCCC	60.57	19608861
48	Acetylation	RDGLQNEKNIVSTPV CCCCCCCCCCCCCCC	60.57	19608861
48	Succinylation	RDGLQNEKNIVSTPV CCCCCCCCCCCCCCC	60.57	-
52	Phosphorylation	QNEKNIVSTPVKIKL CCCCCCCCCCCHHHH	24.17	30266825
53	Phosphorylation	NEKNIVSTPVKIKLI CCCCCCCCCCHHHHH	22.62	30266825
78	Phosphorylation	IETTSFVSPKWVPQM EEECCCCCCCCCCCC	20.82	24275569
80	Methylation	TTSFVSPKWVPQMGD ECCCCCCCCCCCCCC	54.00	-
93	Acetylation	GDHTEVLKGIQKFPG CCHHHHHHHHHHCCC	60.17	23954790
93	Malonylation	GDHTEVLKGIQKFPG CCHHHHHHHHHHCCC	60.17	26320211
93	Ubiquitination	GDHTEVLKGIQKFPG CCHHHHHHHHHHCCC	60.17	19608861
111	Malonylation	PVLTPNLKGFEAAVA CEECCCCCHHHHHHH	69.10	26320211
111	Acetylation	PVLTPNLKGFEAAVA CEECCCCCHHHHHHH	69.10	23236377
137	Malonylation	ASELFTKKNINCSIE HHHHHHCCCCCCCHH	61.06	26320211
137	Succinylation	ASELFTKKNINCSIE HHHHHHCCCCCCCHH	61.06	-
137	Acetylation	ASELFTKKNINCSIE HHHHHHCCCCCCCHH	61.06	23749302
137	Succinylation	ASELFTKKNINCSIE HHHHHHCCCCCCCHH	61.06	-
179	Succinylation	LGCPYEGKISPAKVA CCCCCCCCCCHHHHH	28.24	-
179	Acetylation	LGCPYEGKISPAKVA CCCCCCCCCCHHHHH	28.24	-
179	Succinylation	LGCPYEGKISPAKVA CCCCCCCCCCHHHHH	28.24	-
181	Phosphorylation	CPYEGKISPAKVAEV CCCCCCCCHHHHHHH	23.45	24719451
198	Phosphorylation	KFYSMGCYEISLGDT HHHHCCCEEEECCCC	15.57	22817900
314	Phosphorylation	CQALNRKTSSKVAQA HHHHCCCCCHHHHHH	34.69	26074081
315	Phosphorylation	QALNRKTSSKVAQAT HHHCCCCCHHHHHHH	31.06	26074081
316	Phosphorylation	ALNRKTSSKVAQATC HHCCCCCHHHHHHHH	36.06	26074081
324	Acetylation	KVAQATCKL------ HHHHHHHCC------	53.53	25953088
324	Malonylation	KVAQATCKL------ HHHHHHHCC------	53.53	26320211

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of HMGCL_HUMAN !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of HMGCL_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of HMGCL_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
HES1_HUMAN	HES1	physical	21900206
DNJA1_HUMAN	DNAJA1	physical	21900206
RN126_HUMAN	RNF126	physical	21900206
ATS10_HUMAN	ADAMTS10	physical	21900206
AR6P1_HUMAN	ARL6IP1	physical	21900206
PNPT1_HUMAN	PNPT1	physical	26186194
FOLC_HUMAN	FPGS	physical	26186194
GLSK_HUMAN	GLS	physical	26186194
HEM1_HUMAN	ALAS1	physical	26186194
ACADV_HUMAN	ACADVL	physical	26186194
MGME1_HUMAN	MGME1	physical	26186194
FBX21_HUMAN	FBXO21	physical	26186194
LSM12_HUMAN	LSM12	physical	26344197
TOM40_HUMAN	TOMM40	physical	26344197
FOLC_HUMAN	FPGS	physical	28514442
GLSK_HUMAN	GLS	physical	28514442
HEM1_HUMAN	ALAS1	physical	28514442
ACADV_HUMAN	ACADVL	physical	28514442
MGME1_HUMAN	MGME1	physical	28514442

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• 246450: 3-hydroxy-3-methylglutaryl-CoA lyase deficiency (HMGCLD)
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Acetylation

"Lysine acetylation targets protein complexes and co-regulates majorcellular functions.";
Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.,Olsen J.V., Mann M.;
Science 325:834-840(2009).
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-48, AND MASS SPECTROMETRY.
PubMed: 19608861