GPRK2_DROME - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: GPRK2_DROME
• UniProt AC: P32866
• Protein Name: G protein-coupled receptor kinase 2
• Gene Name: Gprk2
• Organism: Drosophila melanogaster (Fruit fly).
• Sequence Length: 714
• Subcellular Localization: Membrane . Associated with nurse cell and oocyte plasma membranes during much of oogenesis.
• Protein Description: Specifically phosphorylates the activated forms of G protein-coupled receptors (By similarity). Required during oogenesis and embryogenesis; component of a signaling pathway that functions during egg chamber maturation..
• Protein Sequence: MELENIVANTVYLKAREGGSDSNKGKSKKWRKILQFPHISQCINLKDKLDISYGYVIDQQPIGRELFRLFCENKRPVYFRYITFLDEVVKYEIEYISNRIFIGHDIGRRFLDVEAQLELRNGSGGDALDAETQEELLLNSSNANPTETAETEHCNNTTANNCNNINNSNNSQHSSDINHKKLDTRNHNGDDATGNGSSHQDDGDESVKCQEGHDDAEKGGGGEGGGGGKCVPVGGYPDELVLDVLNDDLIAQVRNKLNSGGKDIFAQCVNAVKAFLAGEPFREFESSMYFHRYLQWKWLEAQPITYKTFRMYRVLGKGGFGEVCACQVRATGKMYACKKLEKKRIKKRKGESMVLIEKQILQKINSPFVVNLAYAYETKDALCLVLTIMNGGDLKFHIYNMGGEPGFELERARFYAAEVACGLQHLHKQGIVYRDCKPENILLDDHGHVRISDLGLAVEIPEGEMVRGRVGTVGYMAPEVIDNEKYAFSPDWFSFGCLLYEMIEGQAPFRMRKEKVKREEVDRRVKEDPEKYSSKFNDEAKSMCQQLLAKSIKQRLGCRNGRMGGQDVMAHPFFHSTQLNWRRLEAGMLEPPFVPDPHAVYAKDVLDIEQFSTVKGVNIDESDTNFYTKFNTGSVSISWQNEMMETECFRELNVFGPEECPTPDLQINAAPEPDKAGCFPFRRKKKQPARTQPIPIPEHLLTTSHSVSSTTVES

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
193	Phosphorylation	NHNGDDATGNGSSHQ CCCCCCCCCCCCCCC	37.87	19429919
197	Phosphorylation	DDATGNGSSHQDDGD CCCCCCCCCCCCCCC	28.75	19429919
198	Phosphorylation	DATGNGSSHQDDGDE CCCCCCCCCCCCCCC	26.94	19429919
612	Phosphorylation	VLDIEQFSTVKGVNI CCCHHHCEECCCCCC	31.80	19429919
613	Phosphorylation	LDIEQFSTVKGVNID CCHHHCEECCCCCCC	27.76	28490779
702	Phosphorylation	PIPEHLLTTSHSVSS CCCHHHHCCCCCCCC	32.39	19429919
703	Phosphorylation	IPEHLLTTSHSVSST CCHHHHCCCCCCCCC	25.14	19429919
704	Phosphorylation	PEHLLTTSHSVSSTT CHHHHCCCCCCCCCC	14.45	19429919
706	Phosphorylation	HLLTTSHSVSSTTVE HHHCCCCCCCCCCCC	24.38	19429919
708	Phosphorylation	LTTSHSVSSTTVES- HCCCCCCCCCCCCC-	25.61	19429919
709	Phosphorylation	TTSHSVSSTTVES-- CCCCCCCCCCCCC--	26.41	19429919
710	Phosphorylation	TSHSVSSTTVES--- CCCCCCCCCCCC---	27.62	19429919
711	Phosphorylation	SHSVSSTTVES---- CCCCCCCCCCC----	24.99	19429919
714	Phosphorylation	VSSTTVES------- CCCCCCCC-------	40.98	19429919

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of GPRK2_DROME !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of GPRK2_DROME !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of GPRK2_DROME !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
PDE4B_DROME	dnc	genetic	11319866
PDE4E_DROME	dnc	genetic	11319866
PDE4C_DROME	dnc	genetic	11319866
PDE4A_DROME	dnc	genetic	11319866
FOG_DROME	fog	genetic	24026125
SMO_DROME	smo	genetic	17483466
KAPC_DROME	Pka-C1	genetic	22096079
CACT_DROME	cact	physical	20421637
GPRK2_DROME	Gprk2	physical	20844016

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"Phosphoproteome analysis of Drosophila melanogaster embryos.";
Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
J. Proteome Res. 7:1675-1682(2008).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-612 AND THR-613, ANDMASS SPECTROMETRY.
PubMed: 18327897