DTX3L_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: DTX3L_HUMAN
• UniProt AC: Q8TDB6
• Protein Name: E3 ubiquitin-protein ligase DTX3L
• Gene Name: DTX3L
• Organism: Homo sapiens (Human).
• Sequence Length: 740
• Subcellular Localization: Cytoplasm . Nucleus . Early endosome membrane ; Peripheral membrane protein ; Cytoplasmic side . Lysosome membrane ; Peripheral membrane protein ; Cytoplasmic side . Translocates to the nucleus in response to IFNG or IFNB1 stimulation (PubMed:2647978
• Protein Description: E3 ubiquitin-protein ligase which, in association with ADP-ribosyltransferase PARP9, plays a role in DNA damage repair and in interferon-mediated antiviral responses. [PubMed: 12670957]
• Protein Sequence: MASHLRPPSPLLVRVYKSGPRVRRKLESYFQSSKSSGGGECTVSTQEHEAPGTFRVEFSERAAKERVLKKGEHQILVDEKPVPIFLVPTENSIKKNTRPQISSLTQSQAETPSGDMHQHEGHIPNAVDSCLQKIFLTVTADLNCNLFSKEQRAYITTLCPSIRKMEGHDGIEKVCGDFQDIERIHQFLSEQFLESEQKQQFSPSMTERKPLSQQERDSCISPSEPETKAEQKSNYFEVPLPYFEYFKYICPDKINSIEKRFGVNIEIQESSPNMVCLDFTSSRSGDLEAARESFASEFQKNTEPLKQECVSLADSKQANKFKQELNHQFTKLLIKEKGGELTLLGTQDDISAAKQKISEAFVKIPVKLFAANYMMNVIEVDSAHYKLLETELLQEISEIEKRYDICSKVSEKGQKTCILFESKDRQVDLSVHAYASFIDAFQHASCQLMREVLLLKSLGKERKHLHQTKFADDFRKRHPNVHFVLNQESMTLTGLPNHLAKAKQYVLKGGGMSSLAGKKLKEGHETPMDIDSDDSKAASPPLKGSVSSEASELDKKEKGICVICMDTISNKKVLPKCKHEFCAPCINKAMSYKPICPTCQTSYGIQKGNQPEGSMVFTVSRDSLPGYESFGTIVITYSMKAGIQTEEHPNPGKRYPGIQRTAYLPDNKEGRKVLKLLYRAFDQKLIFTVGYSRVLGVSDVITWNDIHHKTSRFGGPEMYGYPDPSYLKRVKEELKAKGIE

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Acetylation	------MASHLRPPS ------CCCCCCCCC	12.98	19369195
3	Phosphorylation	-----MASHLRPPSP -----CCCCCCCCCC	23.04	29255136
9	Phosphorylation	ASHLRPPSPLLVRVY CCCCCCCCCCEEEEE	30.75	29255136
16	Phosphorylation	SPLLVRVYKSGPRVR CCCEEEEECCCHHHH	6.72	24719451
18	Phosphorylation	LLVRVYKSGPRVRRK CEEEEECCCHHHHHH	36.02	24719451
25	Acetylation	SGPRVRRKLESYFQS CCHHHHHHHHHHHHC	46.15	23954790
25	Malonylation	SGPRVRRKLESYFQS CCHHHHHHHHHHHHC	46.15	26320211
28	Phosphorylation	RVRRKLESYFQSSKS HHHHHHHHHHHCCCC	41.50	29978859
29	Phosphorylation	VRRKLESYFQSSKSS HHHHHHHHHHCCCCC	9.03	23312004
32	Phosphorylation	KLESYFQSSKSSGGG HHHHHHHCCCCCCCC	29.81	29978859
33	Phosphorylation	LESYFQSSKSSGGGE HHHHHHCCCCCCCCC	26.27	23312004
34	Ubiquitination	ESYFQSSKSSGGGEC HHHHHCCCCCCCCCC	54.62	29967540
35	Phosphorylation	SYFQSSKSSGGGECT HHHHCCCCCCCCCCE	35.84	28857561
36	Phosphorylation	YFQSSKSSGGGECTV HHHCCCCCCCCCCEE	45.23	28857561
44	Phosphorylation	GGGECTVSTQEHEAP CCCCCEEECCCCCCC	13.25	22468782
45	Phosphorylation	GGECTVSTQEHEAPG CCCCEEECCCCCCCC	33.37	22468782
80	Ubiquitination	HQILVDEKPVPIFLV CEEEECCCCCCEEEE	47.02	29967540
89	Phosphorylation	VPIFLVPTENSIKKN CCEEEEECCCCCCCC	40.08	27251275
92	Phosphorylation	FLVPTENSIKKNTRP EEEECCCCCCCCCCH	29.56	27251275
95	Ubiquitination	PTENSIKKNTRPQIS ECCCCCCCCCCHHHH	62.98	-
148	Phosphorylation	DLNCNLFSKEQRAYI CCCCCCCCHHHHHHH	38.15	24719451
163 (in isoform 2)	Ubiquitination	-	36.28	21890473
164	Ubiquitination	TLCPSIRKMEGHDGI HHCHHHHHHCCCCCH	39.37	29967540
173	Ubiquitination	EGHDGIEKVCGDFQD CCCCCHHHHHCCHHH	40.69	29967540
189	Phosphorylation	ERIHQFLSEQFLESE HHHHHHHHHHHHHHH	30.55	24732914
195	Phosphorylation	LSEQFLESEQKQQFS HHHHHHHHHHHHCCC	47.69	26074081
197 (in isoform 2)	Ubiquitination	-	59.13	21890473
198	Ubiquitination	QFLESEQKQQFSPSM HHHHHHHHHCCCCCC	41.85	29967540
202	Phosphorylation	SEQKQQFSPSMTERK HHHHHCCCCCCCCCC	16.24	29255136
204	Phosphorylation	QKQQFSPSMTERKPL HHHCCCCCCCCCCCC	36.82	29255136
206	Phosphorylation	QQFSPSMTERKPLSQ HCCCCCCCCCCCCCH	36.66	26329039
209	Ubiquitination	SPSMTERKPLSQQER CCCCCCCCCCCHHHH	44.04	29967540
212	Phosphorylation	MTERKPLSQQERDSC CCCCCCCCHHHHHCC	38.04	26074081
216 (in isoform 2)	Ubiquitination	-	36.48	21890473
218	Phosphorylation	LSQQERDSCISPSEP CCHHHHHCCCCCCCC	21.41	20058876
221	Phosphorylation	QERDSCISPSEPETK HHHHCCCCCCCCCCH	27.05	25159151
223	Phosphorylation	RDSCISPSEPETKAE HHCCCCCCCCCCHHH	60.02	30183078
227	Phosphorylation	ISPSEPETKAEQKSN CCCCCCCCHHHHHCC	46.39	23403867
228	Ubiquitination	SPSEPETKAEQKSNY CCCCCCCHHHHHCCC	48.54	21963094
232	Ubiquitination	PETKAEQKSNYFEVP CCCHHHHHCCCEECC	31.97	22817900
233	Phosphorylation	ETKAEQKSNYFEVPL CCHHHHHCCCEECCC	36.13	28348404
235	Phosphorylation	KAEQKSNYFEVPLPY HHHHHCCCEECCCHH	14.28	22817900
248	Phosphorylation	PYFEYFKYICPDKIN HHHHHHHHHCHHHHH	9.61	22817900
253	Acetylation	FKYICPDKINSIEKR HHHHCHHHHHHHHHH	29.00	25953088
253	Ubiquitination	FKYICPDKINSIEKR HHHHCHHHHHHHHHH	29.00	29967540
256	Phosphorylation	ICPDKINSIEKRFGV HCHHHHHHHHHHHCC	35.28	23401153
259	Ubiquitination	DKINSIEKRFGVNIE HHHHHHHHHHCCCEE	51.89	-
293	Phosphorylation	DLEAARESFASEFQK CHHHHHHHHHHHHHH	22.13	27499020
300	Ubiquitination	SFASEFQKNTEPLKQ HHHHHHHHCCHHHHH	71.77	29967540
306	Acetylation	QKNTEPLKQECVSLA HHCCHHHHHHHHHHH	55.56	26051181
306	Ubiquitination	QKNTEPLKQECVSLA HHCCHHHHHHHHHHH	55.56	29967540
311	Phosphorylation	PLKQECVSLADSKQA HHHHHHHHHHHHHHH	29.51	30108239
315	Phosphorylation	ECVSLADSKQANKFK HHHHHHHHHHHHHHH	22.51	30108239
316	Ubiquitination	CVSLADSKQANKFKQ HHHHHHHHHHHHHHH	54.71	21963094
320	Ubiquitination	ADSKQANKFKQELNH HHHHHHHHHHHHHHH	57.88	22817900
322	Ubiquitination	SKQANKFKQELNHQF HHHHHHHHHHHHHHH	44.46	29967540
331	Ubiquitination	ELNHQFTKLLIKEKG HHHHHHHHHHHHCCC	42.45	29967540
337	Ubiquitination	TKLLIKEKGGELTLL HHHHHHCCCCEEEEE	68.18	29967540
354	Ubiquitination	QDDISAAKQKISEAF HHHHHHHHHHHHHHH	52.41	21963094
356	Ubiquitination	DISAAKQKISEAFVK HHHHHHHHHHHHHHH	47.71	22817900
363	Ubiquitination	KISEAFVKIPVKLFA HHHHHHHHHHHHHHH	34.53	21963094
367	Ubiquitination	AFVKIPVKLFAANYM HHHHHHHHHHHHHHH	32.31	22817900
373	Phosphorylation	VKLFAANYMMNVIEV HHHHHHHHHCCEEEE	7.94	22817900
385	Phosphorylation	IEVDSAHYKLLETEL EEECHHHHHHHHHHH	11.75	22817900
401	Acetylation	QEISEIEKRYDICSK HHHHHHHHHHCHHHH	63.02	26051181
401	Ubiquitination	QEISEIEKRYDICSK HHHHHHHHHHCHHHH	63.02	22505724
408	Acetylation	KRYDICSKVSEKGQK HHHCHHHHHCCCCCE	45.42	25953088
408	Ubiquitination	KRYDICSKVSEKGQK HHHCHHHHHCCCCCE	45.42	22505724
412	Ubiquitination	ICSKVSEKGQKTCIL HHHHHCCCCCEEEEE	60.17	-
415	Ubiquitination	KVSEKGQKTCILFES HHCCCCCEEEEEEEC	54.77	29967540
423	Ubiquitination	TCILFESKDRQVDLS EEEEEECCCCCEEEE	50.13	-
456	Ubiquitination	MREVLLLKSLGKERK HHHHHHHHHHCHHHH	43.53	21890473
460	Ubiquitination	LLLKSLGKERKHLHQ HHHHHHCHHHHHHHH	60.69	23000965
463	Ubiquitination	KSLGKERKHLHQTKF HHHCHHHHHHHHCCC	52.16	29967540
469	Ubiquitination	RKHLHQTKFADDFRK HHHHHHCCCHHHHHH	31.80	29967540
476	Ubiquitination	KFADDFRKRHPNVHF CCHHHHHHHCCCEEE	55.86	29967540
501	Ubiquitination	GLPNHLAKAKQYVLK CCCCHHHHHHHHHHH	63.58	29967540
503	Ubiquitination	PNHLAKAKQYVLKGG CCHHHHHHHHHHHCC	41.26	29967540
508	Ubiquitination	KAKQYVLKGGGMSSL HHHHHHHHCCCHHHH	45.53	29967540
513	Phosphorylation	VLKGGGMSSLAGKKL HHHCCCHHHHCCCCC	26.02	-
514	Phosphorylation	LKGGGMSSLAGKKLK HHCCCHHHHCCCCCC	17.22	28857561
518	Acetylation	GMSSLAGKKLKEGHE CHHHHCCCCCCCCCC	49.69	25953088
518	Ubiquitination	GMSSLAGKKLKEGHE CHHHHCCCCCCCCCC	49.69	29967540
519	Ubiquitination	MSSLAGKKLKEGHET HHHHCCCCCCCCCCC	64.97	-
521	Ubiquitination	SLAGKKLKEGHETPM HHCCCCCCCCCCCCC	71.21	-
526	Phosphorylation	KLKEGHETPMDIDSD CCCCCCCCCCCCCCC	20.45	29255136
532	Phosphorylation	ETPMDIDSDDSKAAS CCCCCCCCCCCCCCC	43.59	29255136
535	Phosphorylation	MDIDSDDSKAASPPL CCCCCCCCCCCCCCC	29.23	30266825
539	Phosphorylation	SDDSKAASPPLKGSV CCCCCCCCCCCCCCC	31.73	23401153
543	Ubiquitination	KAASPPLKGSVSSEA CCCCCCCCCCCCCCH	55.71	33845483
545	O-linked_Glycosylation	ASPPLKGSVSSEASE CCCCCCCCCCCCHHH	19.76	29351928
545	Phosphorylation	ASPPLKGSVSSEASE CCCCCCCCCCCCHHH	19.76	26657352
547	Phosphorylation	PPLKGSVSSEASELD CCCCCCCCCCHHHCC	24.94	23927012
548	Phosphorylation	PLKGSVSSEASELDK CCCCCCCCCHHHCCC	34.72	26657352
551	Phosphorylation	GSVSSEASELDKKEK CCCCCCHHHCCCCCC	34.34	24732914
556	Ubiquitination	EASELDKKEKGICVI CHHHCCCCCCCEEEE	65.41	-
558	Ubiquitination	SELDKKEKGICVICM HHCCCCCCCEEEEEE	63.56	-
567	Phosphorylation	ICVICMDTISNKKVL EEEEEECCCCCCCCC	10.22	27251275
591	Phosphorylation	PCINKAMSYKPICPT HHHHHHHCCCCCCCC	34.56	28857561
592	Phosphorylation	CINKAMSYKPICPTC HHHHHHCCCCCCCCC	14.01	20090780
593	Ubiquitination	INKAMSYKPICPTCQ HHHHHCCCCCCCCCC	22.16	22505724
601	Phosphorylation	PICPTCQTSYGIQKG CCCCCCCHHCCCCCC	26.51	27251275
602	Phosphorylation	ICPTCQTSYGIQKGN CCCCCCHHCCCCCCC	8.76	28857561
603	Phosphorylation	CPTCQTSYGIQKGNQ CCCCCHHCCCCCCCC	22.95	27251275
607	Ubiquitination	QTSYGIQKGNQPEGS CHHCCCCCCCCCCCE	58.89	21963094
614	Phosphorylation	KGNQPEGSMVFTVSR CCCCCCCEEEEEEEC	15.31	27499020
623	Phosphorylation	VFTVSRDSLPGYESF EEEEECCCCCCCCCC	35.04	-
629	Phosphorylation	DSLPGYESFGTIVIT CCCCCCCCCCEEEEE	22.09	27251275
632	Phosphorylation	PGYESFGTIVITYSM CCCCCCCEEEEEEEE	15.29	27251275
653	Ubiquitination	EEHPNPGKRYPGIQR CCCCCCCCCCCCCCC	50.44	21963094
668	Ubiquitination	TAYLPDNKEGRKVLK EEECCCCHHHHHHHH	69.77	23000965
672	Ubiquitination	PDNKEGRKVLKLLYR CCCHHHHHHHHHHHH	63.88	23000965
675	Acetylation	KEGRKVLKLLYRAFD HHHHHHHHHHHHHCC	39.16	25953088
675	Ubiquitination	KEGRKVLKLLYRAFD HHHHHHHHHHHHHCC	39.16	23000965
675 (in isoform 1)	Ubiquitination	-	39.16	21890473
698	Phosphorylation	YSRVLGVSDVITWND CCCCCCCCCEEEHHH	25.10	27251275
709	Ubiquitination	TWNDIHHKTSRFGGP EHHHHCCCCCCCCCH	33.75	22817900
709 (in isoform 1)	Ubiquitination	-	33.75	21890473
719	Phosphorylation	RFGGPEMYGYPDPSY CCCCHHHHCCCCHHH	16.31	23917254
721	Phosphorylation	GGPEMYGYPDPSYLK CCHHHHCCCCHHHHH	6.23	-
728	Ubiquitination	YPDPSYLKRVKEELK CCCHHHHHHHHHHHH	47.08	23000965
728 (in isoform 1)	Ubiquitination	-	47.08	21890473
731	Ubiquitination	PSYLKRVKEELKAKG HHHHHHHHHHHHHCC	49.88	23000965

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of DTX3L_HUMAN !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of DTX3L_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of DTX3L_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
UB2D1_HUMAN	UBE2D1	physical	12670957
UB2D2_HUMAN	UBE2D2	physical	12670957
UB2D3_HUMAN	UBE2D3	physical	12670957
DTX3L_HUMAN	DTX3L	physical	12670957
UB2D1_HUMAN	UBE2D1	physical	19818714
UB2D2_HUMAN	UBE2D2	physical	19818714
RN111_HUMAN	RNF111	physical	22493164
TRIM9_HUMAN	TRIM9	physical	22493164
ITCH_HUMAN	ITCH	physical	24790097
HGS_HUMAN	HGS	physical	24790097
STAM1_HUMAN	STAM	physical	24790097
DTX3L_HUMAN	DTX3L	physical	24790097
PARP9_HUMAN	PARP9	physical	24886089
PAR14_HUMAN	PARP14	physical	24886089
INGR1_HUMAN	IFNGR1	physical	24886089
JAK1_HUMAN	JAK1	physical	24886089
JAK2_HUMAN	JAK2	physical	24886089
STAT1_HUMAN	STAT1	physical	24886089
H2B1J_HUMAN	HIST1H2BJ	physical	26479788
H2AV_HUMAN	H2AFV	physical	26479788
STAT1_HUMAN	STAT1	physical	26479788
PARP9_HUMAN	PARP9	physical	26479788
PARP9_HUMAN	PARP9	physical	28514442

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• There are no disease associations of PTM sites.
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"Large-scale proteomics analysis of the human kinome.";
Oppermann F.S., Gnad F., Olsen J.V., Hornberger R., Greff Z., Keri G.,Mann M., Daub H.;
Mol. Cell. Proteomics 8:1751-1764(2009).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-9, AND MASSSPECTROMETRY.
PubMed: 19369195

"A quantitative atlas of mitotic phosphorylation.";
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,Elledge S.J., Gygi S.P.;
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-221, AND MASSSPECTROMETRY.
PubMed: 18669648

"Kinase-selective enrichment enables quantitative phosphoproteomics ofthe kinome across the cell cycle.";
Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R.,Greff Z., Keri G., Stemmann O., Mann M.;
Mol. Cell 31:438-448(2008).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-9 AND SER-202, AND MASSSPECTROMETRY.
PubMed: 18691976