DPOE3_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: DPOE3_HUMAN
• UniProt AC: Q9NRF9
• Protein Name: DNA polymerase epsilon subunit 3
• Gene Name: POLE3
• Organism: Homo sapiens (Human).
• Sequence Length: 147
• Subcellular Localization: Nucleus .
• Protein Description: Forms a complex with DNA polymerase epsilon subunit CHRAC1 and binds naked DNA, which is then incorporated into chromatin, aided by the nucleosome-remodeling activity of ISWI/SNF2H and ACF1..
• Protein Sequence: MAERPEDLNLPNAVITRIIKEALPDGVNISKEARSAISRAASVFVLYATSCANNFAMKGKRKTLNASDVLSAMEEMEFQRFVTPLKEALEAYRREQKGKKEASEQKKKDKDKKTDSEEQDKSRDEDNDEDEERLEEEEQNEEEEVDN

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Acetylation	------MAERPEDLN ------CCCCHHHCC	22.85	19413330
20	Ubiquitination	AVITRIIKEALPDGV HHHHHHHHHHCCCCC	34.27	-
31	Acetylation	PDGVNISKEARSAIS CCCCCCCHHHHHHHH	52.15	25953088
31	Ubiquitination	PDGVNISKEARSAIS CCCCCCCHHHHHHHH	52.15	21890473
35	Phosphorylation	NISKEARSAISRAAS CCCHHHHHHHHHHHH	36.68	20860994
38	Phosphorylation	KEARSAISRAASVFV HHHHHHHHHHHHHHH	18.64	20860994
62	Ubiquitination	FAMKGKRKTLNASDV CHHCCCCCCCCHHHH	61.93	-
63	Phosphorylation	AMKGKRKTLNASDVL HHCCCCCCCCHHHHH	29.31	22617229
67	Phosphorylation	KRKTLNASDVLSAME CCCCCCHHHHHHHHH	27.46	30576142
71	Phosphorylation	LNASDVLSAMEEMEF CCHHHHHHHHHHHHH	25.97	20068231
83	Phosphorylation	MEFQRFVTPLKEALE HHHHHHHHHHHHHHH	21.86	18669648
86	Ubiquitination	QRFVTPLKEALEAYR HHHHHHHHHHHHHHH	41.99	21890473
92	Phosphorylation	LKEALEAYRREQKGK HHHHHHHHHHHHHCC	10.79	23312004
114	Phosphorylation	KKDKDKKTDSEEQDK HHHHCCCCCHHHHHH	50.96	28985074
116	Phosphorylation	DKDKKTDSEEQDKSR HHCCCCCHHHHHHHC	48.54	30576142
122	Phosphorylation	DSEEQDKSRDEDNDE CHHHHHHHCCCCCHH	53.94	26503892
123	Methylation	SEEQDKSRDEDNDED HHHHHHHCCCCCHHH	58.01	115488093
133	Methylation	DNDEDEERLEEEEQN CCHHHHHHHHHHHHH	45.40	115488085

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of DPOE3_HUMAN !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of DPOE3_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of DPOE3_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
SMCA5_HUMAN	SMARCA5	physical	10880450
BAZ1A_HUMAN	BAZ1A	physical	10880450
CHRC1_HUMAN	CHRAC1	physical	10880450
BAZ1A_HUMAN	BAZ1A	physical	14759371
SMCA5_HUMAN	SMARCA5	physical	14759371
DPOE4_HUMAN	POLE4	physical	10801849
DPOE2_HUMAN	POLE2	physical	10801849
DPOE1_HUMAN	POLE	physical	21705323
A4_HUMAN	APP	physical	21832049
DPOE4_HUMAN	POLE4	physical	22939629
ANXA6_HUMAN	ANXA6	physical	22863883
SIAS_HUMAN	NANS	physical	22863883
CHRC1_HUMAN	CHRAC1	physical	25416956
ATAD2_HUMAN	ATAD2	physical	26344197
DPOE1_HUMAN	POLE	physical	26344197
CHRC1_HUMAN	CHRAC1	physical	21516116

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• There are no disease associations of PTM sites.
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• DB00242: Cladribine

Related Literatures of Post-Translational Modification

Acetylation

"Lys-N and trypsin cover complementary parts of the phosphoproteome ina refined SCX-based approach.";
Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J.,Mohammed S.;
Anal. Chem. 81:4493-4501(2009).
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, AND MASS SPECTROMETRY.
PubMed: 19413330

Phosphorylation

"A quantitative atlas of mitotic phosphorylation.";
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,Elledge S.J., Gygi S.P.;
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-83 AND SER-122, AND MASSSPECTROMETRY.
PubMed: 18669648

"Kinase-selective enrichment enables quantitative phosphoproteomics ofthe kinome across the cell cycle.";
Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R.,Greff Z., Keri G., Stemmann O., Mann M.;
Mol. Cell 31:438-448(2008).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-122, AND MASSSPECTROMETRY.
PubMed: 18691976

"Global, in vivo, and site-specific phosphorylation dynamics insignaling networks.";
Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P.,Mann M.;
Cell 127:635-648(2006).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-122, AND MASSSPECTROMETRY.
PubMed: 17081983