dbPTM

CREM_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	CREM_HUMAN
UniProt AC	Q03060
Protein Name	cAMP-responsive element modulator
Gene Name	CREM
Organism	Homo sapiens (Human).
Sequence Length	361
Subcellular Localization	Nucleus. Isoform 6: Cytoplasm. Nucleus.
Protein Description	Transcriptional regulator that binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. Isoforms are either transcriptional activators or repressors. Plays a role in spermatogenesis and is involved in spermatid maturation. [PubMed: 10373550; Isoform 6: May play a role in the regulation of the circadian clock: acts as a transcriptional repressor of the core circadian component PER1 by directly binding to cAMP response elements in its promoter.]
Protein Sequence	MSKCARKKYIKTNPRQMTMETVESQHDGSITASLTESKSAHVQTQTGQNSIPALAQVSVAGSGTRRGSPAVTLVQLPSGQTIHVQGVIQTPQPWVIQSSEIHTVQVAAIAETDESAESEGVIDSHKRREILSRRPSYRKILNELSSDVPGVPKIEEERSEEEGTPPSIATMAVPTSIYQTSTGQYIAIAQGGTIQISNPGSDGVQGLQALTMTNSGAPPPGATIVQYAAQSADGTQQFFVPGSQVVVQDEETELAPSHMAAATGDMPTYQIRAPTAALPQGVVMAASPGSLHSPQQLAEEATRKRELRLMKNREAAKECRRRKKEYVKCLESRVAVLEVQNKKLIEELETLKDICSPKTDY

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
8 (in isoform 14)	Phosphorylation	-	46.81	8404858
8 (in isoform 7)	Phosphorylation	-	46.81	8404858
12 (in isoform 10)	Phosphorylation	-	40.68	24275569
12 (in isoform 8)	Phosphorylation	-	40.68	24275569
18	Phosphorylation	KTNPRQMTMETVESQ CCCCCCCEEEHHHHC	11.97	24043423
21	Phosphorylation	PRQMTMETVESQHDG CCCCEEEHHHHCCCC	20.82	24043423
24	Phosphorylation	MTMETVESQHDGSIT CEEEHHHHCCCCCEE	28.60	24043423
27 (in isoform 24)	Phosphorylation	-	44.92	22210691
27 (in isoform 27)	Phosphorylation	-	44.92	22210691
29	Phosphorylation	VESQHDGSITASLTE HHHCCCCCEEEEEEC	23.87	20068231
31	Phosphorylation	SQHDGSITASLTESK HCCCCCEEEEEECCC	16.34	24043423
33	Phosphorylation	HDGSITASLTESKSA CCCCEEEEEECCCCC	27.46	24043423
33 (in isoform 12)	Phosphorylation	-	27.46	8404858
33 (in isoform 13)	Phosphorylation	-	27.46	8404858
33 (in isoform 22)	Phosphorylation	-	27.46	8404858
35	Phosphorylation	GSITASLTESKSAHV CCEEEEEECCCCCEE	35.40	24043423
37	Phosphorylation	ITASLTESKSAHVQT EEEEEECCCCCEEEE	27.03	24043423
39	Phosphorylation	ASLTESKSAHVQTQT EEEECCCCCEEEECC	32.65	20068231
44	Phosphorylation	SKSAHVQTQTGQNSI CCCCEEEECCCCCCC	27.32	18669648
47 (in isoform 25)	Phosphorylation	-	18.17	8404858
47 (in isoform 30)	Phosphorylation	-	18.17	8404858
50	Phosphorylation	QTQTGQNSIPALAQV EECCCCCCCCCEEEE	23.28	18669648
63	Phosphorylation	QVSVAGSGTRRGSPA EEEECCCCCCCCCCC	23.48	8404858
63	Ubiquitination	QVSVAGSGTRRGSPA EEEECCCCCCCCCCC	23.48	33845483
63 (in isoform 15)	Phosphorylation	-	23.48	8404858
63 (in isoform 16)	Phosphorylation	-	23.48	8404858
63 (in isoform 2)	Phosphorylation	-	23.48	8404858
63 (in isoform 26)	Phosphorylation	-	23.48	8404858
63 (in isoform 31)	Phosphorylation	-	23.48	8404858
63 (in isoform 32)	Phosphorylation	-	23.48	8404858
65	Ubiquitination	SVAGSGTRRGSPAVT EECCCCCCCCCCCEE	44.48	24816145
66	Phosphorylation	VAGSGTRRGSPAVTL ECCCCCCCCCCCEEE	50.18	8404858
70	Ubiquitination	GTRRGSPAVTLVQLP CCCCCCCCEEEEECC	14.50	32015554
71	Ubiquitination	TRRGSPAVTLVQLPS CCCCCCCEEEEECCC	4.89	24816145
75	Phosphorylation	SPAVTLVQLPSGQTI CCCEEEEECCCCCEE	51.25	8404858
75	Ubiquitination	SPAVTLVQLPSGQTI CCCEEEEECCCCCEE	51.25	24816145
76	Ubiquitination	PAVTLVQLPSGQTIH CCEEEEECCCCCEEE	2.58	32015554
78	Ubiquitination	VTLVQLPSGQTIHVQ EEEEECCCCCEEEEE	52.72	24816145
80	Ubiquitination	LVQLPSGQTIHVQGV EEECCCCCEEEEEEE	41.44	32015554
81 (in isoform 1)	O-linked_Glycosylation	-	19.31	29485866
83	Phosphorylation	LPSGQTIHVQGVIQT CCCCCEEEEEEEEEC	14.83	8404858
83	Ubiquitination	LPSGQTIHVQGVIQT CCCCCEEEEEEEEEC	14.83	24816145
84	Ubiquitination	PSGQTIHVQGVIQTP CCCCEEEEEEEEECC	4.72	23000965
87	Phosphorylation	QTIHVQGVIQTPQPW CEEEEEEEEECCCCE	1.31	8404858
88	Ubiquitination	TIHVQGVIQTPQPWV EEEEEEEEECCCCEE	4.89	32015554
90 (in isoform 1)	O-linked_Glycosylation	-	16.87	29485866
90	Ubiquitination	HVQGVIQTPQPWVIQ EEEEEEECCCCEEEE	16.87	23000965
91	Ubiquitination	VQGVIQTPQPWVIQS EEEEEECCCCEEEEC	23.12	21890473
91	Ubiquitination	VQGVIQTPQPWVIQS EEEEEECCCCEEEEC	23.12	21890473
94	Ubiquitination	VIQTPQPWVIQSSEI EEECCCCEEEECCCE	8.46	23000965
94 (in isoform 30)	Phosphorylation	-	8.46	28348404
96	Phosphorylation	QTPQPWVIQSSEIHT ECCCCEEEECCCEEE	2.65	8404858
97	Phosphorylation	TPQPWVIQSSEIHTV CCCCEEEECCCEEEE	31.14	8404858
97	Ubiquitination	TPQPWVIQSSEIHTV CCCCEEEECCCEEEE	31.14	21890473
97	Ubiquitination	TPQPWVIQSSEIHTV CCCCEEEECCCEEEE	31.14	21890473
97 (in isoform 9)	Ubiquitination	-	31.14	21890473
99 (in isoform 30)	Phosphorylation	-	24.97	28348404
100	Ubiquitination	PWVIQSSEIHTVQVA CEEEECCCEEEEEEE	44.00	23000965
101	Ubiquitination	WVIQSSEIHTVQVAA EEEECCCEEEEEEEE	3.40	21890473
101	Ubiquitination	WVIQSSEIHTVQVAA EEEECCCEEEEEEEE	3.40	21890473
102	Ubiquitination	VIQSSEIHTVQVAAI EEECCCEEEEEEEEE	18.88	23000965
102	Phosphorylation	VIQSSEIHTVQVAAI EEECCCEEEEEEEEE	18.88	-
104	Ubiquitination	QSSEIHTVQVAAIAE ECCCEEEEEEEEEEE	2.39	21890473
107	Ubiquitination	EIHTVQVAAIAETDE CEEEEEEEEEEECCC	3.98	23000965
109	Ubiquitination	HTVQVAAIAETDESA EEEEEEEEEECCCCH	2.28	21890473
109 (in isoform 8)	Ubiquitination	-	2.28	21890473
109	Ubiquitination	HTVQVAAIAETDESA EEEEEEEEEECCCCH	2.28	21890473
110	Phosphorylation	TVQVAAIAETDESAE EEEEEEEEECCCCHH	14.65	8404858
110 (in isoform 2)	Phosphorylation	-	14.65	28348404
110 (in isoform 32)	Phosphorylation	-	14.65	28348404
112	Phosphorylation	QVAAIAETDESAESE EEEEEEECCCCHHHC	35.72	8404858
112	Ubiquitination	QVAAIAETDESAESE EEEEEEECCCCHHHC	35.72	23000965
115	Phosphorylation	AIAETDESAESEGVI EEEECCCCHHHCCCC	39.90	8404858
115 (in isoform 2)	Phosphorylation	-	39.90	28348404
115 (in isoform 32)	Phosphorylation	-	39.90	28348404
116	Ubiquitination	IAETDESAESEGVID EEECCCCHHHCCCCC	23.45	21890473
116	Phosphorylation	IAETDESAESEGVID EEECCCCHHHCCCCC	23.45	8404858
119	Ubiquitination	TDESAESEGVIDSHK CCCCHHHCCCCCHHH	49.32	23000965
124	Phosphorylation	ESEGVIDSHKRREIL HHCCCCCHHHHHHHH	21.56	8404858
126	Ubiquitination	EGVIDSHKRREILSR CCCCCHHHHHHHHHC	58.04	21890473
129	Ubiquitination	IDSHKRREILSRRPS CCHHHHHHHHHCCHH	52.92	23000965
129	Phosphorylation	IDSHKRREILSRRPS CCHHHHHHHHHCCHH	52.92	23186163
131	Ubiquitination	SHKRREILSRRPSYR HHHHHHHHHCCHHHH	2.53	23000965
132	Phosphorylation	HKRREILSRRPSYRK HHHHHHHHCCHHHHH	31.72	26074081
136	Phosphorylation	EILSRRPSYRKILNE HHHHCCHHHHHHHHH	35.44	27273156
137	Phosphorylation	ILSRRPSYRKILNEL HHHCCHHHHHHHHHH	20.71	26074081
138	Ubiquitination	LSRRPSYRKILNELS HHCCHHHHHHHHHHC	24.71	21890473
141	Ubiquitination	RPSYRKILNELSSDV CHHHHHHHHHHCCCC	4.58	23000965
145	Phosphorylation	RKILNELSSDVPGVP HHHHHHHCCCCCCCC	20.50	30266825
146	Phosphorylation	KILNELSSDVPGVPK HHHHHHCCCCCCCCC	56.08	30266825
159	Phosphorylation	PKIEEERSEEEGTPP CCHHHHHCCCCCCCC	53.27	8404858
160	Ubiquitination	KIEEERSEEEGTPPS CHHHHHCCCCCCCCC	67.31	23000965
167	Ubiquitination	EEEGTPPSIATMAVP CCCCCCCCEEEEECC	26.51	21890473
170	Ubiquitination	GTPPSIATMAVPTSI CCCCCEEEEECCCEE	12.18	23000965
181	Phosphorylation	PTSIYQTSTGQYIAI CCEEEECCCCCEEEE	18.27	20068231
183	Ubiquitination	SIYQTSTGQYIAIAQ EEEECCCCCEEEEEE	20.33	24816145
199	Ubiquitination	GTIQISNPGSDGVQG CEEEECCCCCCCCCH	36.55	24816145
202	Ubiquitination	QISNPGSDGVQGLQA EECCCCCCCCCHHEE	68.17	23000965
204	Ubiquitination	SNPGSDGVQGLQALT CCCCCCCCCHHEEEE	4.97	32015554
209	Ubiquitination	DGVQGLQALTMTNSG CCCCHHEEEEECCCC	15.28	21890473
211	Phosphorylation	VQGLQALTMTNSGAP CCHHEEEEECCCCCC	25.60	18669648
211	Ubiquitination	VQGLQALTMTNSGAP CCHHEEEEECCCCCC	25.60	24816145
212	Ubiquitination	QGLQALTMTNSGAPP CHHEEEEECCCCCCC	3.29	23000965
216	Ubiquitination	ALTMTNSGAPPPGAT EEEECCCCCCCCCCE	44.14	32015554
218	Ubiquitination	TMTNSGAPPPGATIV EECCCCCCCCCCEEE	38.86	23000965
225	Ubiquitination	PPPGATIVQYAAQSA CCCCCEEEEEEEECC	2.86	21890473
225	Ubiquitination	PPPGATIVQYAAQSA CCCCCEEEEEEEECC	2.86	21890473
226	Phosphorylation	PPGATIVQYAAQSAD CCCCEEEEEEEECCC	20.49	20068231
228	Ubiquitination	GATIVQYAAQSADGT CCEEEEEEEECCCCC	5.07	23000965
229	Phosphorylation	ATIVQYAAQSADGTQ CEEEEEEEECCCCCE	10.03	18669648
230	Ubiquitination	TIVQYAAQSADGTQQ EEEEEEEECCCCCEE	30.86	23000965
232	Phosphorylation	VQYAAQSADGTQQFF EEEEEECCCCCEEEE	13.74	20068231
237	Ubiquitination	QSADGTQQFFVPGSQ ECCCCCEEEEECCCE	33.32	21890473
237	Ubiquitination	QSADGTQQFFVPGSQ ECCCCCEEEEECCCE	33.32	21890473
237	Ubiquitination	QSADGTQQFFVPGSQ ECCCCCEEEEECCCE	33.32	21890473
240	Ubiquitination	DGTQQFFVPGSQVVV CCCEEEEECCCEEEE	5.76	23000965
241	Phosphorylation	GTQQFFVPGSQVVVQ CCEEEEECCCEEEEC	31.40	18669648
246	Ubiquitination	FVPGSQVVVQDEETE EECCCEEEECCCCCC	2.19	24816145
262	Ubiquitination	APSHMAAATGDMPTY CCHHHHHHCCCCCEE	11.54	24816145
265	Ubiquitination	HMAAATGDMPTYQIR HHHHHCCCCCEEEEE	35.22	23000965
267	Ubiquitination	AAATGDMPTYQIRAP HHHCCCCCEEEEECC	32.47	32015554
271	Phosphorylation	GDMPTYQIRAPTAAL CCCCEEEEECCCCCC	2.60	18220336
272	Ubiquitination	DMPTYQIRAPTAALP CCCEEEEECCCCCCC	20.99	21890473
274	Ubiquitination	PTYQIRAPTAALPQG CEEEEECCCCCCCCC	16.84	24816145
274	Phosphorylation	PTYQIRAPTAALPQG CEEEEECCCCCCCCC	16.84	18220336
274 (in isoform 21)	Ubiquitination	-	16.84	21890473
275	Phosphorylation	TYQIRAPTAALPQGV EEEEECCCCCCCCCE	24.47	24732914
275	Ubiquitination	TYQIRAPTAALPQGV EEEEECCCCCCCCCE	24.47	23000965
277	Phosphorylation	QIRAPTAALPQGVVM EEECCCCCCCCCEEE	23.42	18220336
281	Ubiquitination	PTAALPQGVVMAASP CCCCCCCCEEEEECC	16.43	23000965
286	Phosphorylation	PQGVVMAASPGSLHS CCCEEEEECCCCCCC	9.73	18669648
287	Phosphorylation	QGVVMAASPGSLHSP CCEEEEECCCCCCCH	21.91	22167270
288	Ubiquitination	GVVMAASPGSLHSPQ CEEEEECCCCCCCHH	31.50	21890473
290	Phosphorylation	VMAASPGSLHSPQQL EEEECCCCCCCHHHH	26.71	22167270
291	Ubiquitination	MAASPGSLHSPQQLA EEECCCCCCCHHHHH	6.01	23000965
293	Phosphorylation	ASPGSLHSPQQLAEE ECCCCCCCHHHHHHH	29.69	19664994
293	Ubiquitination	ASPGSLHSPQQLAEE ECCCCCCCHHHHHHH	29.69	23000965
300	Ubiquitination	SPQQLAEEATRKREL CHHHHHHHHHHHHHH	50.13	21890473
302	Phosphorylation	QQLAEEATRKRELRL HHHHHHHHHHHHHHH	39.75	21712546
303	Ubiquitination	QLAEEATRKRELRLM HHHHHHHHHHHHHHH	42.98	23000965
330 (in isoform 6)	Ubiquitination	-	5.77	-
337 (in isoform 6)	Ubiquitination	-	3.73	21890473
350	Phosphorylation	KLIEELETLKDICSP HHHHHHHHHHHHHCC	52.30	24719451
356	Phosphorylation	ETLKDICSPKTDY-- HHHHHHHCCCCCC--	29.75	24719451
361	Phosphorylation	ICSPKTDY------- HHCCCCCC-------	26.19	24719451

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
271	S	Phosphorylation	Kinase	CDK1	P06493	PSP
277	S	Phosphorylation	Kinase	CDK1	P06493	PSP
277	S	Phosphorylation	Kinase	MAPK3	P27361	GPS
-	K	Ubiquitination	E3 ubiquitin ligase	CDC34	P49427	PMID:22199232
-	K	Ubiquitination	E3 ubiquitin ligase	STUB1	Q9UNE7	PMID:22267842

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
116	S	Phosphorylation		-
Phosphorylated on Ser-116 by TSSK4 in vitro.

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of CREM_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
TBP_HUMAN	TBP	physical	10409662
TAF4_HUMAN	TAF4	physical	10409662
FHL5_HUMAN	FHL5	physical	10086359
HDAC1_HUMAN	HDAC1	physical	17056544
EMP3_HUMAN	EMP3	physical	21988832
SP100_HUMAN	SP100	physical	21988832
UBC9_HUMAN	UBE2I	physical	21988832
PIAS3_HUMAN	PIAS3	physical	21988832

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
There are no disease associations of PTM sites.
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of CREM_HUMAN

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"A quantitative atlas of mitotic phosphorylation."; Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,Elledge S.J., Gygi S.P.; Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-287 AND SER-293, ANDMASS SPECTROMETRY.	18669648 [Abstract]
"Combining protein-based IMAC, peptide-based IMAC, and MudPIT forefficient phosphoproteomic analysis."; Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D.,Yates J.R. III; J. Proteome Res. 7:1346-1351(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-287; SER-290 ANDSER-293, AND MASS SPECTROMETRY.	18220336 [Abstract]

show