APAF_HUMAN - dbPTM
APAF_HUMAN - PTM Information in dbPTM
Basic Information of Protein
UniProt ID APAF_HUMAN
UniProt AC O14727
Protein Name Apoptotic protease-activating factor 1
Gene Name APAF1
Organism Homo sapiens (Human).
Sequence Length 1248
Subcellular Localization Cytoplasm .
Protein Description Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis..
Protein Sequence MDAKARNCLLQHREALEKDIKTSYIMDHMISDGFLTISEEEKVRNEPTQQQRAAMLIKMILKKDNDSYVSFYNALLHEGYKDLAALLHDGIPVVSSSSGKDSVSGITSYVRTVLCEGGVPQRPVVFVTRKKLVNAIQQKLSKLKGEPGWVTIHGMAGCGKSVLAAEAVRDHSLLEGCFPGGVHWVSVGKQDKSGLLMKLQNLCTRLDQDESFSQRLPLNIEEAKDRLRILMLRKHPRSLLILDDVWDSWVLKAFDSQCQILLTTRDKSVTDSVMGPKYVVPVESSLGKEKGLEILSLFVNMKKADLPEQAHSIIKECKGSPLVVSLIGALLRDFPNRWEYYLKQLQNKQFKRIRKSSSYDYEALDEAMSISVEMLREDIKDYYTDLSILQKDVKVPTKVLCILWDMETEEVEDILQEFVNKSLLFCDRNGKSFRYYLHDLQVDFLTEKNCSQLQDLHKKIITQFQRYHQPHTLSPDQEDCMYWYNFLAYHMASAKMHKELCALMFSLDWIKAKTELVGPAHLIHEFVEYRHILDEKDCAVSENFQEFLSLNGHLLGRQPFPNIVQLGLCEPETSEVYQQAKLQAKQEVDNGMLYLEWINKKNITNLSRLVVRPHTDAVYHACFSEDGQRIASCGADKTLQVFKAETGEKLLEIKAHEDEVLCCAFSTDDRFIATCSVDKKVKIWNSMTGELVHTYDEHSEQVNCCHFTNSSHHLLLATGSSDCFLKLWDLNQKECRNTMFGHTNSVNHCRFSPDDKLLASCSADGTLKLWDATSANERKSINVKQFFLNLEDPQEDMEVIVKCCSWSADGARIMVAAKNKIFLFDIHTSGLLGEIHTGHHSTIQYCDFSPQNHLAVVALSQYCVELWNTDSRSKVADCRGHLSWVHGVMFSPDGSSFLTSSDDQTIRLWETKKVCKNSAVMLKQEVDVVFQENEVMVLAVDHIRRLQLINGRTGQIDYLTEAQVSCCCLSPHLQYIAFGDENGAIEILELVNNRIFQSRFQHKKTVWHIQFTADEKTLISSSDDAEIQVWNWQLDKCIFLRGHQETVKDFRLLKNSRLLSWSFDGTVKVWNIITGNKEKDFVCHQGTVLSCDISHDATKFSSTSADKTAKIWSFDLLLPLHELRGHNGCVRCSAFSVDSTLLATGDDNGEIRIWNVSNGELLHLCAPLSEEGAATHGGWVTDLCFSPDGKMLISAGGYIKWWNVVTGESSQTFYTNGTNLKKIHVSPDFKTYVTVDNLGILYILQTLE
Overview of Protein Modification Sites with Functional and Structural Information
Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations Modification Substrate Peptides
&
Secondary Structure		 ASA (%) Reference Orthologous
Protein Cluster
24PhosphorylationEKDIKTSYIMDHMIS
HHHHHHHHHHHHHHC		12.90-
31PhosphorylationYIMDHMISDGFLTIS
HHHHHHHCCCCEECC		24.89-
48PhosphorylationEKVRNEPTQQQRAAM
HHHCCCCCHHHHHHH		32.6027470641
63UbiquitinationLIKMILKKDNDSYVS
HHHHHHCCCCCCHHH		59.3229967540
95PhosphorylationHDGIPVVSSSSGKDS
HCCCCEEECCCCCCC		25.4128348404
96PhosphorylationDGIPVVSSSSGKDSV
CCCCEEECCCCCCCC		19.5329954749
97PhosphorylationGIPVVSSSSGKDSVS
CCCEEECCCCCCCCC		35.2928348404
98PhosphorylationIPVVSSSSGKDSVSG
CCEEECCCCCCCCCC		52.3828348404
120UbiquitinationVLCEGGVPQRPVVFV
EECCCCCCCCCEEEE		27.9529967540
128UbiquitinationQRPVVFVTRKKLVNA
CCCEEEEEHHHHHHH		25.8829967540
131UbiquitinationVVFVTRKKLVNAIQQ
EEEEEHHHHHHHHHH		55.1429967540
133UbiquitinationFVTRKKLVNAIQQKL
EEEHHHHHHHHHHHH		6.6329967540
139UbiquitinationLVNAIQQKLSKLKGE
HHHHHHHHHHHCCCC		38.0529967540
144UbiquitinationQQKLSKLKGEPGWVT
HHHHHHCCCCCCEEE		66.0329967540
161PhosphorylationGMAGCGKSVLAAEAV
ECCCCCHHHHHHHHH		13.98-
178UbiquitinationHSLLEGCFPGGVHWV
CCHHCCCCCCCEEEE		10.4129967540
181UbiquitinationLEGCFPGGVHWVSVG
HCCCCCCCEEEEEEC		14.3529967540
187UbiquitinationGGVHWVSVGKQDKSG
CCEEEEEECCCCCCH		8.8729967540
189UbiquitinationVHWVSVGKQDKSGLL
EEEEEECCCCCCHHH		53.8429967540
192UbiquitinationVSVGKQDKSGLLMKL
EEECCCCCCHHHHHH		43.8829967540
198UbiquitinationDKSGLLMKLQNLCTR
CCCHHHHHHHHHHHH		47.0629967540
204PhosphorylationMKLQNLCTRLDQDES
HHHHHHHHHCCCCCC		37.1222817900
213UbiquitinationLDQDESFSQRLPLNI
CCCCCCHHHCCCCCH		24.5429967540
213 (in isoform 2)Ubiquitination-24.5421906983
213 (in isoform 3)Ubiquitination-24.5421890473
224UbiquitinationPLNIEEAKDRLRILM
CCCHHHHHHHHHHHH		46.8121906983
224 (in isoform 1)Ubiquitination-46.8121906983
224 (in isoform 4)Ubiquitination-46.8121890473
224 (in isoform 5)Ubiquitination-46.8121890473
224 (in isoform 6)Ubiquitination-46.8121906983
238PhosphorylationMLRKHPRSLLILDDV
HHHCCCCCEEEECCC		31.7925850435
248PhosphorylationILDDVWDSWVLKAFD
EECCCCHHHHHHHHH		11.9025850435
256UbiquitinationWVLKAFDSQCQILLT
HHHHHHHCCCEEEEE		26.8029967540
266UbiquitinationQILLTTRDKSVTDSV
EEEEEECCCCCCCCC		45.5529967540
267UbiquitinationILLTTRDKSVTDSVM
EEEEECCCCCCCCCC		43.4629967540
268PhosphorylationLLTTRDKSVTDSVMG
EEEECCCCCCCCCCC		34.2422246185
270PhosphorylationTTRDKSVTDSVMGPK
EECCCCCCCCCCCCE		29.6424905233
272PhosphorylationRDKSVTDSVMGPKYV
CCCCCCCCCCCCEEE		12.4924905233
277UbiquitinationTDSVMGPKYVVPVES
CCCCCCCEEEEECCC		44.9722817900
277 (in isoform 2)Ubiquitination-44.9721906983
277 (in isoform 3)Ubiquitination-44.9721890473
278PhosphorylationDSVMGPKYVVPVESS
CCCCCCEEEEECCCC		15.1527642862
279UbiquitinationSVMGPKYVVPVESSL
CCCCCEEEEECCCCC		4.9822817900
288MalonylationPVESSLGKEKGLEIL
ECCCCCCHHHCHHHH		62.6426320211
288UbiquitinationPVESSLGKEKGLEIL
ECCCCCCHHHCHHHH		62.6422817900
288 (in isoform 1)Ubiquitination-62.6421906983
288 (in isoform 4)Ubiquitination-62.6421890473
288 (in isoform 5)Ubiquitination-62.6421890473
288 (in isoform 6)Ubiquitination-62.6421906983
290UbiquitinationESSLGKEKGLEILSL
CCCCCHHHCHHHHHH		72.7422817900
292UbiquitinationSLGKEKGLEILSLFV
CCCHHHCHHHHHHHH		5.6829967540
303MalonylationSLFVNMKKADLPEQA
HHHHCCHHCCCHHHH		36.0432601280
303UbiquitinationSLFVNMKKADLPEQA
HHHHCCHHCCCHHHH		36.0429967540
304UbiquitinationLFVNMKKADLPEQAH
HHHCCHHCCCHHHHH		20.3232015554
312PhosphorylationDLPEQAHSIIKECKG
CCHHHHHHHHHHCCC		29.0824719451
315UbiquitinationEQAHSIIKECKGSPL
HHHHHHHHHCCCCHH		56.2232015554
320PhosphorylationIIKECKGSPLVVSLI
HHHHCCCCHHHHHHH		10.68-
337UbiquitinationLLRDFPNRWEYYLKQ
HHCCCCCHHHHHHHH		28.0429967540
348UbiquitinationYLKQLQNKQFKRIRK
HHHHHHHHHHHHHHC		44.1229967540
357PhosphorylationFKRIRKSSSYDYEAL
HHHHHCCCCCCHHHH		35.6322246185
369PhosphorylationEALDEAMSISVEMLR
HHHHHHHCCCHHHHH		20.82-
369UbiquitinationEALDEAMSISVEMLR
HHHHHHHCCCHHHHH		20.8229967540
371PhosphorylationLDEAMSISVEMLRED
HHHHHCCCHHHHHHH		12.52-
380UbiquitinationEMLREDIKDYYTDLS
HHHHHHHHHHHHHHH		52.5829967540
382PhosphorylationLREDIKDYYTDLSIL
HHHHHHHHHHHHHHH		11.8529978859
383PhosphorylationREDIKDYYTDLSILQ
HHHHHHHHHHHHHHH		12.1529978859
384PhosphorylationEDIKDYYTDLSILQK
HHHHHHHHHHHHHHC		25.0029978859
387PhosphorylationKDYYTDLSILQKDVK
HHHHHHHHHHHCCCC		24.8629978859
391UbiquitinationTDLSILQKDVKVPTK
HHHHHHHCCCCCCCE		62.1329967540
432PhosphorylationFCDRNGKSFRYYLHD
EECCCCCEEEEEEHH		19.1628348404
435PhosphorylationRNGKSFRYYLHDLQV
CCCCEEEEEEHHHCC		14.3628348404
436PhosphorylationNGKSFRYYLHDLQVD
CCCEEEEEEHHHCCH		8.0328348404
447UbiquitinationLQVDFLTEKNCSQLQ
HCCHHHCCCCHHHHH		44.4029967540
458UbiquitinationSQLQDLHKKIITQFQ
HHHHHHHHHHHHHHH		53.6229967540
506PhosphorylationELCALMFSLDWIKAK
HHHHHHHCHHHHHHH		16.0824719451
570UbiquitinationIVQLGLCEPETSEVY
EEEECCCCCCCHHHH		51.7329967540
581UbiquitinationSEVYQQAKLQAKQEV
HHHHHHHHHHHHHHH		36.4729967540
589UbiquitinationLQAKQEVDNGMLYLE
HHHHHHHHCCEEEEE		45.2529967540
590UbiquitinationQAKQEVDNGMLYLEW
HHHHHHHCCEEEEEE		42.8429967540
600UbiquitinationLYLEWINKKNITNLS
EEEEECCCCCCCCHH		37.7629967540
601UbiquitinationYLEWINKKNITNLSR
EEEECCCCCCCCHHH		49.4429967540
619PhosphorylationRPHTDAVYHACFSED
CCCCHHHEEEEECCC		5.8527642862
626UbiquitinationYHACFSEDGQRIASC
EEEEECCCCCEEECC		58.2829967540
632UbiquitinationEDGQRIASCGADKTL
CCCCEEECCCCCCCE		15.6529967540
637UbiquitinationIASCGADKTLQVFKA
EECCCCCCCEEEEEC		51.0729967540
638UbiquitinationASCGADKTLQVFKAE
ECCCCCCCEEEEECC		23.9929967540
643AcetylationDKTLQVFKAETGEKL
CCCEEEEECCCCCCE		46.597661749
643UbiquitinationDKTLQVFKAETGEKL
CCCEEEEECCCCCCE		46.5929967540
649UbiquitinationFKAETGEKLLEIKAH
EECCCCCCEEEEEEC		60.7729967540
654AcetylationGEKLLEIKAHEDEVL
CCCEEEEEECCCCEE		34.0218527667
679AcetylationIATCSVDKKVKIWNS
EEEEECCCCEEEEEC		58.2627452117
722UbiquitinationLLATGSSDCFLKLWD
EEEECCCCHHHHHHC		28.9632015554
733UbiquitinationKLWDLNQKECRNTMF
HHHCCCHHHHHHHHC		58.6832015554
743PhosphorylationRNTMFGHTNSVNHCR
HHHHCCCCCCCCCCC		29.5728857561
745UbiquitinationTMFGHTNSVNHCRFS
HHCCCCCCCCCCCCC		26.0032015554
752PhosphorylationSVNHCRFSPDDKLLA
CCCCCCCCCCCCCCC		14.2021815630
756UbiquitinationCRFSPDDKLLASCSA
CCCCCCCCCCCCCCC		53.4332015554
757UbiquitinationRFSPDDKLLASCSAD
CCCCCCCCCCCCCCC		6.5929967540
762PhosphorylationDKLLASCSADGTLKL
CCCCCCCCCCCCEEE		27.2223403867
768UbiquitinationCSADGTLKLWDATSA
CCCCCCEEEEECCCC		47.5829967540
807UbiquitinationIVKCCSWSADGARIM
HHHHHHCCCCCCEEE		10.5329967540
809UbiquitinationKCCSWSADGARIMVA
HHHHCCCCCCEEEEE		45.8521890473
809 (in isoform 3)Ubiquitination-45.8521890473
818UbiquitinationARIMVAAKNKIFLFD
CEEEEEECCEEEEEE		49.3829967540
820UbiquitinationIMVAAKNKIFLFDIH
EEEEECCEEEEEEEE		33.8421890473
820 (in isoform 4)Ubiquitination-33.8421890473
828 (in isoform 5)Ubiquitination-25.4821890473
901PhosphorylationGSSFLTSSDDQTIRL
CCCCCCCCCCCCEEE		39.72-
905PhosphorylationLTSSDDQTIRLWETK
CCCCCCCCEEEEEEH		18.30-
916UbiquitinationWETKKVCKNSAVMLK
EEEHHHHCCCCEEEE		57.85-
994UbiquitinationILELVNNRIFQSRFQ
HHHHHCCHHHHHHHC		26.3729967540
1005UbiquitinationSRFQHKKTVWHIQFT
HHHCCCCEEEEEEEE		33.3629967540
1023UbiquitinationKTLISSSDDAEIQVW
CEEECCCCCCEEEEE		62.1729967540
1025UbiquitinationLISSSDDAEIQVWNW
EECCCCCCEEEEEEE		22.3029967540
1034UbiquitinationIQVWNWQLDKCIFLR
EEEEEEECCEEEEEC		5.2629967540
1036UbiquitinationVWNWQLDKCIFLRGH
EEEEECCEEEEECCC		37.5129967540
1037UbiquitinationWNWQLDKCIFLRGHQ
EEEECCEEEEECCCH		2.3829967540
1045UbiquitinationIFLRGHQETVKDFRL
EEECCCHHHHHHHHH		50.3129967540
1048UbiquitinationRGHQETVKDFRLLKN
CCCHHHHHHHHHHCC		59.5829967540
1053UbiquitinationTVKDFRLLKNSRLLS
HHHHHHHHCCCEEEE		4.2629967540
1056UbiquitinationDFRLLKNSRLLSWSF
HHHHHCCCEEEEEEE		23.7929967540
1064UbiquitinationRLLSWSFDGTVKVWN
EEEEEEECCEEEEEE		46.4629967540
1066UbiquitinationLSWSFDGTVKVWNII
EEEEECCEEEEEEEE		20.9929967540
1068UbiquitinationWSFDGTVKVWNIITG
EEECCEEEEEEEECC		41.1729967540
1077UbiquitinationWNIITGNKEKDFVCH
EEEECCCCCCCEEEE		68.4429967540
1079UbiquitinationIITGNKEKDFVCHQG
EECCCCCCCEEEECC		59.0629967540
1088UbiquitinationFVCHQGTVLSCDISH
EEEECCEEEEEECCC		4.7829967540
1096UbiquitinationLSCDISHDATKFSST
EEEECCCCCCCCCCC		49.4929967540
1099UbiquitinationDISHDATKFSSTSAD
ECCCCCCCCCCCCCC		44.9929967540
1103PhosphorylationDATKFSSTSADKTAK
CCCCCCCCCCCCCCE		27.08-
1107UbiquitinationFSSTSADKTAKIWSF
CCCCCCCCCCEEEEE		50.5329967540
1108PhosphorylationSSTSADKTAKIWSFD
CCCCCCCCCEEEEEE		33.46-
1168UbiquitinationLLHLCAPLSEEGAAT
EEEEEEECCCCCCCC		5.5329967540
1179UbiquitinationGAATHGGWVTDLCFS
CCCCCCCEEEEEEEC		8.4029967540
1211UbiquitinationVVTGESSQTFYTNGT
ECCCCCCCEEEECCC		44.9329967540
1222UbiquitinationTNGTNLKKIHVSPDF
ECCCCCEEEEECCCC		41.0129967540
1231PhosphorylationHVSPDFKTYVTVDNL
EECCCCCCEEEECHH		24.1826546556
1232PhosphorylationVSPDFKTYVTVDNLG
ECCCCCCEEEECHHH		8.5026546556
Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)
Modified Location Modified Residue Modification Type of Upstream Proteins Gene Name of Upstream Proteins UniProt AC of Upstream Proteins Sources
268SPhosphorylationKinaseRPS6KA1Q15418
GPS
357SPhosphorylationKinaseRPS6KA1Q15418
GPS
Functions of PTM Sites
Modified Location Modified Residue Modification Function Reference

Oops, there are no descriptions of PTM sites of APAF_HUMAN !!
Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location Modification Variant Position
(Distance <= 10) 		Residue Change SAP Related Disease Reference

Oops, there are no SNP-PTM records of APAF_HUMAN !!
Protein-Protein Interaction
Interacting Protein Gene Name Interaction Type PPI Reference Domain-Domain Interactions
MTNB_HUMANAPIPphysical
15262985
CASP9_HUMANCASP9physical
15262985
NLRP1_HUMANNLRP1physical
11113115
HSP74_HUMANHSPA4physical
10934467
HS90A_HUMANHSP90AA1physical
10934467
CASP9_HUMANCASP9physical
9539746
CASP4_HUMANCASP4physical
9539746
CASP8_HUMANCASP8physical
9539746
CASP9_HUMANCASP9physical
9488720
B2CL1_HUMANBCL2L1physical
9488720
CASP9_HUMANCASP9physical
11242052
XIAP_HUMANXIAPphysical
11242052
CASP9_HUMANCASP9physical
20829884
ENPL_HUMANHSP90B1physical
25737303
CASP9_HUMANCASP9physical
27476169
CASP9_HUMANCASP9physical
27197231
Drug and Disease Associations
Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
There are no disease associations of PTM sites.
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
DB00171Adenosine triphosphate
Regulatory Network of APAF_HUMAN

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Related Literatures of Post-Translational Modification
Phosphorylation
ReferencePubMed
"Global, in vivo, and site-specific phosphorylation dynamics insignaling networks.";
Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P.,Mann M.;
Cell 127:635-648(2006).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-204; SER-238 ANDSER-248, AND MASS SPECTROMETRY.

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