AMN1_YEAST - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: AMN1_YEAST
• UniProt AC: P38285
• Protein Name: Antagonist of mitotic exit network protein 1
• Gene Name: AMN1
• Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
• Sequence Length: 549
• Subcellular Localization: Cytoplasm . Nucleus .
• Protein Description: Negative regulator of the mitotic exit network (MEN), required for multiple cell cycle checkpoints. Acts in the daughter cell to inhibit the mitotic exit pathway once MEN has executed its function. Through its binding ability to TEM1, interferes with the TEM1-CDC5 association, required for CDC5 kinase activation and MEN activation. Required for daughter cell separation and chromosome stability. Involved in copper sensitivity..
• Protein Sequence: MKLERVSSNGSFKRGRDIQSLESPCTRPLKKMSPSPSFTSLKMEKPFKDIVRKYGGHLHQSSYNPGSSKVELVRPDLSLKTDQSFLQSSVQTTPNKKSCNEYLSTPEATPLKNTATENAWATSRVVSASSLSIVTPTEIKNILVDEFSELKLGQPLTAQHQRSHAVFEIPEIVENIIKMIVSLESANIPKERPCLRRNPQSYEHSLLMYKDEERAKKAWSAAQQLRDPPLVGHKEKKQGALFSCMMVNRLWLNVTRPFLFKSLHFKSVHNFKEFLRTSQETTQVMRPSHFILHKLHQVTQPDIERLSRMECQNLKWLEFYVCPRITPPLSWFDNLHKLEKLIIPGNKNIDDNFLLRLSQSIPNLKHLVLRACDNVSDSGVVCIALNCPKLKTFNIGRHRRGNLITSVSLVALGKYTQVETVGFAGCDVDDAGIWEFARLNGKNVERLSLNSCRLLTDYSLPILFALNSFPNLAVLEIRNLDKITDVRHFVKYNLWKKSLDAPILIEACERITKLIDQEENRVKRINSLVALKDMTAWVNADDEIENNVD

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
7	Phosphorylation	-MKLERVSSNGSFKR -CCCEEECCCCCCCC	25.06	19823750
8	Phosphorylation	MKLERVSSNGSFKRG CCCEEECCCCCCCCC	42.21	20377248
11	Phosphorylation	ERVSSNGSFKRGRDI EEECCCCCCCCCCCC	31.67	21440633
33	Phosphorylation	TRPLKKMSPSPSFTS CCCCCCCCCCCCCCC	30.98	21440633
35	Phosphorylation	PLKKMSPSPSFTSLK CCCCCCCCCCCCCCC	26.35	21440633
37	Phosphorylation	KKMSPSPSFTSLKME CCCCCCCCCCCCCCC	45.56	21440633
40	Phosphorylation	SPSPSFTSLKMEKPF CCCCCCCCCCCCCCH	24.79	21440633
53	Ubiquitination	PFKDIVRKYGGHLHQ CHHHHHHHHCCCCCC	37.04	17644757
69	Ubiquitination	SYNPGSSKVELVRPD CCCCCCCCEEEECCC	41.23	17644757
80	Ubiquitination	VRPDLSLKTDQSFLQ ECCCCCCCCCHHHHH	46.83	17644757
127	Phosphorylation	WATSRVVSASSLSIV HHHCEEEEHHHCCCC	21.41	27017623
135	Phosphorylation	ASSLSIVTPTEIKNI HHHCCCCCCHHHHHH	23.87	28152593
178	Ubiquitination	EIVENIIKMIVSLES HHHHHHHHHHHHHHH	20.83	17644757
201	Phosphorylation	CLRRNPQSYEHSLLM CHHCCCCCHHCCEEC	33.47	27017623
202	Phosphorylation	LRRNPQSYEHSLLMY HHCCCCCHHCCEECC	16.65	26447709
205	Phosphorylation	NPQSYEHSLLMYKDE CCCCHHCCEECCCCH	15.97	26447709
209	Phosphorylation	YEHSLLMYKDEERAK HHCCEECCCCHHHHH	18.43	26447709
267	Phosphorylation	FKSLHFKSVHNFKEF HHHCCCCCCCCHHHH	27.89	23749301
294	Ubiquitination	PSHFILHKLHQVTQP HHHHHHHHHHHCCCC	44.19	23749301
299	Phosphorylation	LHKLHQVTQPDIERL HHHHHHCCCCCHHHH	28.02	27017623
315	Ubiquitination	RMECQNLKWLEFYVC HHHHCCCCEEEEEEC	58.23	23749301
347	Ubiquitination	KLIIPGNKNIDDNFL EEEECCCCCCCCCHH	62.28	23749301
360	Phosphorylation	FLLRLSQSIPNLKHL HHHHHHCCCCCHHHH	36.32	23749301
365	Ubiquitination	SQSIPNLKHLVLRAC HCCCCCHHHHHHHHC	41.34	23749301
389	Ubiquitination	CIALNCPKLKTFNIG EEEECCCCCCEEECC	65.89	23749301
391	Ubiquitination	ALNCPKLKTFNIGRH EECCCCCCEEECCCC	58.14	22817900
442	Ubiquitination	EFARLNGKNVERLSL HHHHHCCCCCEEEEC	57.66	23749301
482	Ubiquitination	LEIRNLDKITDVRHF EEECCCCHHHCCHHH	51.15	23749301
527	Phosphorylation	NRVKRINSLVALKDM HHHHHHHHHHHHHHC	23.11	21440633

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of AMN1_YEAST !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of AMN1_YEAST !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of AMN1_YEAST !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
SSZ1_YEAST	SSZ1	physical	11805826
ZUO1_YEAST	ZUO1	physical	11805826
TEM1_YEAST	TEM1	physical	12628189
SSZ1_YEAST	SSZ1	physical	16429126
ZUO1_YEAST	ZUO1	physical	16429126
TEM1_YEAST	TEM1	genetic	12628189
2AAA_YEAST	TPD3	genetic	16079183
BFA1_YEAST	BFA1	genetic	16079183
2ABA_YEAST	CDC55	genetic	16079183
PP2A1_YEAST	PPH21	genetic	16079183
PP2A2_YEAST	PPH22	genetic	16079183
BUB2_YEAST	BUB2	genetic	16079183

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.