AAKG3_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: AAKG3_HUMAN
• UniProt AC: Q9UGI9
• Protein Name: 5'-AMP-activated protein kinase subunit gamma-3
• Gene Name: PRKAG3
• Organism: Homo sapiens (Human).
• Sequence Length: 489
• Protein Description: AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Gamma non-catalytic subunit mediates binding to AMP, ADP and ATP, leading to activate or inhibit AMPK: AMP-binding results in allosteric activation of alpha catalytic subunit (PRKAA1 or PRKAA2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits. ADP also stimulates phosphorylation, without stimulating already phosphorylated catalytic subunit. ATP promotes dephosphorylation of catalytic subunit, rendering the AMPK enzyme inactive..
• Protein Sequence: MEPGLEHALRRTPSWSSLGGSEHQEMSFLEQENSSSWPSPAVTSSSERIRGKRRAKALRWTRQKSVEEGEPPGQGEGPRSRPAAESTGLEATFPKTTPLAQADPAGVGTPPTGWDCLPSDCTASAAGSSTDDVELATEFPATEAWECELEGLLEERPALCLSPQAPFPKLGWDDELRKPGAQIYMRFMQEHTCYDAMATSSKLVIFDTMLEIKKAFFALVANGVRAAPLWDSKKQSFVGMLTITDFILVLHRYYRSPLVQIYEIEQHKIETWREIYLQGCFKPLVSISPNDSLFEAVYTLIKNRIHRLPVLDPVSGNVLHILTHKRLLKFLHIFGSLLPRPSFLYRTIQDLGIGTFRDLAVVLETAPILTALDIFVDRRVSALPVVNECGQVVGLYSRFDVIHLAAQQTYNHLDMSVGEALRQRTLCLEGVLSCQPHESLGEVIDRIAREQVHRLVLVDETQHLLGVVSLSDILQALVLSPAGIDALGA

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
27	Phosphorylation	GSEHQEMSFLEQENS CCCCCCHHHHHCCCC	26.40	23663014
34	Phosphorylation	SFLEQENSSSWPSPA HHHHCCCCCCCCCCC	25.62	23663014
35	Phosphorylation	FLEQENSSSWPSPAV HHHCCCCCCCCCCCC	48.26	23663014
36	Phosphorylation	LEQENSSSWPSPAVT HHCCCCCCCCCCCCC	42.71	23663014
39	Phosphorylation	ENSSSWPSPAVTSSS CCCCCCCCCCCCCCC	21.16	23663014
43	Phosphorylation	SWPSPAVTSSSERIR CCCCCCCCCCCHHHH	25.62	23663014
44	Phosphorylation	WPSPAVTSSSERIRG CCCCCCCCCCHHHHC	25.85	23663014
45	Phosphorylation	PSPAVTSSSERIRGK CCCCCCCCCHHHHCH	27.18	23663014
46	Phosphorylation	SPAVTSSSERIRGKR CCCCCCCCHHHHCHH	30.57	23663014
65	Phosphorylation	LRWTRQKSVEEGEPP HHHHHHHCHHCCCCC	26.82	26437602
80	Phosphorylation	GQGEGPRSRPAAEST CCCCCCCCCCCHHHC	45.83	26437602
86	Phosphorylation	RSRPAAESTGLEATF CCCCCHHHCCCCCCC	24.05	26437602
192	Phosphorylation	MRFMQEHTCYDAMAT HHHHHHCCHHHHHHC	16.41	23663014
194	Phosphorylation	FMQEHTCYDAMATSS HHHHCCHHHHHHCCC	14.11	23663014
199	Phosphorylation	TCYDAMATSSKLVIF CHHHHHHCCCCEEEE	22.05	23663014
233	Ubiquitination	AAPLWDSKKQSFVGM EECCCCCCCCCCEEE	52.97	-
236	Phosphorylation	LWDSKKQSFVGMLTI CCCCCCCCCEEEEHH	30.68	24667141
323	Phosphorylation	GNVLHILTHKRLLKF CCCHHHHCHHHHHHH	25.42	50565027
355	Phosphorylation	IQDLGIGTFRDLAVV HHHHCCCCHHHHHHH	17.36	24719451
397	Phosphorylation	GQVVGLYSRFDVIHL HCEEEHHHHHHHHHH	31.36	24719451

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of AAKG3_HUMAN !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of AAKG3_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of AAKG3_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
AAKB1_HUMAN	PRKAB1	physical	10698692
AAKB2_HUMAN	PRKAB2	physical	10698692
CRYAB_HUMAN	CRYAB	physical	19616115
LMCD1_HUMAN	LMCD1	physical	19616115
NGRN_HUMAN	NGRN	physical	19616115
KLC1_HUMAN	KLC1	physical	21915095
BRCA1_HUMAN	BRCA1	physical	25184681
AAKB2_HUMAN	PRKAB2	physical	28514442
AAKB1_HUMAN	PRKAB1	physical	28514442
AAPK1_HUMAN	PRKAA1	physical	28514442
AAPK2_HUMAN	PRKAA2	physical	28514442
CLH2_HUMAN	CLTCL1	physical	28514442
PFD1_HUMAN	PFDN1	physical	28514442

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.