TBK1_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: TBK1_MOUSE
• UniProt AC: Q9WUN2
• Protein Name: Serine/threonine-protein kinase TBK1
• Gene Name: Tbk1
• Organism: Mus musculus (Mouse).
• Sequence Length: 729
• Subcellular Localization: Cytoplasm. Upon mitogen stimulation or triggering of the immune system, TBK1 is recruited to the exocyst by EXOC2..
• Protein Description: Serine/threonine kinase that plays an essential role in regulating inflammatory responses to foreign agents. [PubMed: 10581243]
• Protein Sequence: MQSTSNHLWLLSDILGQGATANVFRGRHKKTGDLYAVKVFNNISFLRPVDVQMREFEVLKKLNHKNIVKLFAIEEETTTRHKVLIMEFCPCGSLYTVLEEPSNAYGLPESEFLIVLRDVVGGMNHLRENGIVHRDIKPGNIMRVIGEDGQSVYKLTDFGAARELEDDEQFVSLYGTEEYLHPDMYERAVLRKDHQKKYGATVDLWSVGVTFYHAATGSLPFRPFEGPRRNKEVMYKIITGKPSGAISGVQKAENGPIDWSGDMPLSCSLSQGLQALLTPVLANILEADQEKCWGFDQFFAETSDVLHRMVIHVFSLQHMTAHKIYIHSYNTAAVFHELVYKQTKIVSSNQELIYEGRRLVLELGRLAQHFPKTTEENPIFVTSREQLNTVGLRYEKISLPKIHPRYDLDGDASMAKAVTGVVCYACRTASTLLLYQELMRKGVRWLVELVKDDYNETVHKKTEVVITLDFCIRNIEKTVKVYEKLMKVNLEAAELGEISDIHTKLLRLSSSQGTIESSLQDISSRLSPGGLLADTWAHQEGTHPRDRNVEKLQVLLNCITEIYYQFKKDKAERRLAYNEEQIHKFDKQKLYYHATKAMSHFSEECVRKYEAFKDKSEEWMRKMLHLRKQLLSLTNQCFDIEEEVSKYQDYTNELQETLPQKMLAASGGVKHAMAPIYPSSNTLVEMTLGMKKLKEEMEGVVKELAENNHILERFGSLTMDGGLRNVDCL

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
30	Acetylation	VFRGRHKKTGDLYAV EECCCCCCCCCEEEE	52.17	112807629
38	Ubiquitination	TGDLYAVKVFNNISF CCCEEEEEEECCCCE	32.70	-
154	Acetylation	EDGQSVYKLTDFGAA CCCCEEEEECCCCCC	42.63	112807625
172	Phosphorylation	EDDEQFVSLYGTEEY CCHHHHHHHHCCHHH	19.70	22322096
236	Acetylation	RNKEVMYKIITGKPS CCCEEEEEHHCCCCC	14.99	112807631
241	Acetylation	MYKIITGKPSGAISG EEEHHCCCCCCCCCC	27.62	112807621
251	Acetylation	GAISGVQKAENGPID CCCCCEEECCCCCCC	56.09	112807627
398	Phosphorylation	GLRYEKISLPKIHPR CCEEEECCCCCCCCC	50.08	24719451
499	Phosphorylation	AAELGEISDIHTKLL HHHHCCCCHHHHHHH	26.64	24719451
510	Phosphorylation	TKLLRLSSSQGTIES HHHHHHHCCCCCHHH	31.13	25338131
511	Phosphorylation	KLLRLSSSQGTIESS HHHHHHCCCCCHHHH	29.29	25338131
646	Acetylation	DIEEEVSKYQDYTNE CHHHHHHHHHHHHHH	52.70	112807623
691	Acetylation	VEMTLGMKKLKEEME HHHHHCCHHHHHHHH	53.83	112807633
716	Phosphorylation	HILERFGSLTMDGGL CHHHHHHCEECCCCC	20.25	28725479
718	Phosphorylation	LERFGSLTMDGGLRN HHHHHCEECCCCCCC	18.21	28066266

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
172	S	Phosphorylation	Kinase	IKKB	O88351	Uniprot
179	Y	Phosphorylation	Kinase	PYK2	Q9QVP9	PSP

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
30	K	Phosphorylation	Phosphorylation requires homodimerization and ubiquitination at Lys-30 and Lys-401.	-
30	K	ubiquitylation	'Lys-63'-linked polyubiquitination by MIB1 after RNA virus infection, or by NRDP1 after LPS stimulation at Lys-30 and Lys-401, participates in kinase activation.	-
30	K	ubiquitylation	'Lys-63'-linked polyubiquitination by RNF128 at Lys-30 and Lys-401 leads to the activation of antiviral responses.	-
30	K	ubiquitylation	Phosphorylation requires homodimerization and ubiquitination at Lys-30 and Lys-401.	-
172	S	Phosphorylation	Autophosphorylation at Ser-172 activates the kinase, and is an essential step for virus-triggered signaling.	23396211
172	S	Phosphorylation	Dephosphorylated at Ser-172 by PPM1B and this negatively regulates its role in mediating antiviral response.	23396211
172	S	Phosphorylation	Phosphorylated by IKBKB/IKKB at Ser-172.	23396211
401	K	Phosphorylation	Phosphorylation requires homodimerization and ubiquitination at Lys-30 and Lys-401.	-
401	K	ubiquitylation	'Lys-63'-linked polyubiquitination by MIB1 after RNA virus infection, or by NRDP1 after LPS stimulation at Lys-30 and Lys-401, participates in kinase activation.	-
401	K	ubiquitylation	'Lys-63'-linked polyubiquitination by RNF128 at Lys-30 and Lys-401 leads to the activation of antiviral responses.	-
401	K	ubiquitylation	Phosphorylation requires homodimerization and ubiquitination at Lys-30 and Lys-401.	-
670	K	ubiquitylation	'Lys-48'-linked polyubiquitination at Lys-670 by DTX4 leads to proteasomal degradation.	-

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of TBK1_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
TANK_HUMAN	TANK	physical	10581243
IRF1_MOUSE	Irf1	physical	17468758
OPTN_MOUSE	Optn	physical	21862579
IRF3_MOUSE	Irf3	physical	21862579
TRAIP_MOUSE	Traip	physical	22945920
TBK1_MOUSE	Tbk1	physical	23746807
IRF1_HUMAN	IRF1	physical	23746807
AZI2_MOUSE	Azi2	physical	21903422
MIB1_MOUSE	Mib1	physical	21903422
OPTN_HUMAN	OPTN	physical	26376340

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.