SPAG8_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: SPAG8_HUMAN
• UniProt AC: Q99932
• Protein Name: Sperm-associated antigen 8
• Gene Name: SPAG8
• Organism: Homo sapiens (Human).
• Sequence Length: 426
• Subcellular Localization: Cytoplasm . Nucleus . Cytoplasmic vesicle, secretory vesicle, acrosome . Cytoplasm, cytoskeleton, microtubule organizing center . Cytoplasm, cytoskeleton, spindle . In mature sperm cells, detected in the acrosomal region of the head and in the middle
• Protein Description: Plays a role in spermatogenesis by enhancing the binding of CREM isoform tau to its coactivator FHL5 and increasing the FHL5-regulated transcriptional activation of CREM isoform tau (By similarity). Involved in the acrosome reaction and in binding of sperm to the zona pellucida (By similarity). Plays a role in regulation of the cell cycle by controlling progression through the G2/M phase, possibly by delaying the activation of CDK1 which is required for entry into mitosis. [PubMed: 19548270 May play a role in fertility and microtubule formation through interaction with RANBP9]
• Protein Sequence: METNESTEGSRSRSRSLDIQPSSEGLGPTSEPFPSSDDSPRSALAAATAAAAAAASAAAATAAFTTAKAAALSTKTPAPCSEFMEPSSDPSLLGEPCAGPGFTHNIAHGSLGFEPVYVSCIAQDTCTTTDHSSNPGPVPGSSSGPVLGSSSGAGHGSGSGSGPGCGSVPGSGSGPGPGSGPGSGPGHGSGSHPGPASGPGPDTGPDSELSPCIPPGFRNLVADRVPNYTSWSQHCPWEPQKQPPWEFLQVLEPGARGLWKPPDIKGKLMVCYETLPRGQCLLYNWEEERATNHLDQVPSMQDGSESFFFRHGHRGLLTMQLKSPMPSSTTQKDSYQPPGNVYWPLRGKREAMLEMLLQHQICKEVQAEQEPTRKLFEVESVTHHDYRMELAQAGTPAPTKPHDYRQEQPETFWIQRAPQLPVCEGD

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
10	Phosphorylation	TNESTEGSRSRSRSL CCCCCCCCCCCCCCC	22.35	-
14	Phosphorylation	TEGSRSRSRSLDIQP CCCCCCCCCCCCCCC	27.71	27966365
16	Phosphorylation	GSRSRSRSLDIQPSS CCCCCCCCCCCCCCC	31.47	25693802
22	Phosphorylation	RSLDIQPSSEGLGPT CCCCCCCCCCCCCCC	25.33	30622161
23	Phosphorylation	SLDIQPSSEGLGPTS CCCCCCCCCCCCCCC	42.22	30622161
228	Phosphorylation	VADRVPNYTSWSQHC HCCCCCCCCCHHHCC	8.97	25693802
229	Phosphorylation	ADRVPNYTSWSQHCP CCCCCCCCCHHHCCC	30.29	25693802
230	Phosphorylation	DRVPNYTSWSQHCPW CCCCCCCCHHHCCCC	17.86	25693802
232	Phosphorylation	VPNYTSWSQHCPWEP CCCCCCHHHCCCCCC	15.15	25693802
267	Acetylation	KPPDIKGKLMVCYET CCCCCCCCEEEEEEE	28.54	25038526
438 (in isoform 3)	Phosphorylation	-		25219547
440 (in isoform 3)	Phosphorylation	-		25219547
441 (in isoform 3)	Phosphorylation	-		25219547
480 (in isoform 2)	Phosphorylation	-		25332170

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of SPAG8_HUMAN !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of SPAG8_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of SPAG8_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
RANB9_HUMAN	RANBP9	physical	15014887
CARM1_HUMAN	CARM1	physical	23455924
KDM3B_HUMAN	KDM3B	physical	23455924
JMJD4_HUMAN	JMJD4	physical	23455924
ANM1_HUMAN	PRMT1	physical	23455924
ANM2_HUMAN	PRMT2	physical	23455924
ANM5_HUMAN	PRMT5	physical	23455924
NSD3_HUMAN	WHSC1L1	physical	23455924
DTX2_HUMAN	DTX2	physical	25416956
KLH32_HUMAN	KLHL32	physical	25416956

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.