SHAN3_RAT - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: SHAN3_RAT
• UniProt AC: Q9JLU4
• Protein Name: SH3 and multiple ankyrin repeat domains protein 3
• Gene Name: Shank3
• Organism: Rattus norvegicus (Rat).
• Sequence Length: 1740
• Subcellular Localization: Cytoplasm. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell projection, dendritic spine. In neuronal cells, extends into the region subjacent to the PSD.
• Protein Description: Major scaffold postsynaptic density protein which interacts with multiple proteins and complexes to orchestrate the dendritic spine and synapse formation, maturation and maintenance. Interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors via complexes with GKAP/PSD-95 and HOMER, respectively, and the actin-based cytoskeleton. Plays a role in the structural and functional organization of the dendritic spine and synaptic junction through the interaction with Arp2/3 and WAVE1 complex as well as the promotion of the F-actin clusters. By way of this control of actin dynamics, participates in the regulation of developing neurons growth cone motility and the NMDA receptor-signaling. Also modulates GRIA1 exocytosis and GRM5/MGLUR5 expression and signaling to control the AMPA and metabotropic glutamate receptor-mediated synaptic transmission and plasticity. May be required at an early stage of synapse formation and be inhibited by IGF1 to promote synapse maturation..
• Protein Sequence: MDGPGASAVVVRVGIPDLQQTKCLRLDPTAPVWAAKQRVLCALNHSLQDALNYGLFQPPSRGRAGKFLDEERLLQDYPPNLDTPLPYLEFRYKRRVYAQNLIDDKQFAKLHTKANLKKFMDYVQLHSTDKVARLLDKGLDPNFHDPDSGECPLSLAAQLDNATDLLKVLRNGGAHLDFRTRDGLTAVHCATRQRNAGALTTLLDLGASPDYKDSRGLTPLYHSALGGGDALCCELLLHDHAQLGTTDENGWQEIHQACRFGHVQHLEHLLFYGANMGAQNASGNTALHICALYNQESCARVLLFRGANKDVRNYNSQTAFQVAIIAGNFELAEVIKTHKDSDVVPFRETPSYAKRRRLAGPSGLASPRPLQRSASDINLKGDQPAASPGPTLRSLPHQLLLQRLQEEKDRDRDGEQENDISGPSAGRGGHSKISPSGPGGSGPAPGPGPASPAPPAPPPRGPKRKLYSAVPGRKFIAVKAHSPQGEGEIPLHRGEAVKVLSIGEGGFWEGTVKGRTGWFPADCVEEVQMRQYDTRHETREDRTKRLFRHYTVGSYDSLTSHSDYVIDDKVAILQKRDHEGFGFVLRGAKAETPIEEFTPTPAFPALQYLESVDVEGVAWKAGLRTGDFLIEVNGVNVVKVGHKQVVGLIRQGGNRLVMKVVSVTRKPEEDSARRRAPPPPKRAPSTTLTLRSKSMTAELEELASIRRRKGEKLDEILAVAAEPTLRPDIADADSRAATVKQRPTSRRITPAEISSLFERQGLPGPEKLPGSLRKGIPRTKSVGEDEKLASLLEGRFPRSTSMQDTVREGRGIPPPPQTAPPPPPAPYYFDSGPPPTFSPPPPPPGRAYDTVRSSFKPGLEARLGAGAAGLYDSGTPLGPLPYPERQKRARSMIILQDSAPEVGDVPRPAPAATPPERPKRRPRPSGPDSPYANLGAFSASLFAPSKPQRRKSPLVKQLQVEDAQERAALAVGSPGPVGGSFAREPSPTHRGPRPGGLDYSSGEGLGLTFGGPSPGPVKERRLEERRRSTVFLSVGAIEGSPPSADLPSLQPSRSIDERLLGTGATTGRDLLLPSPVSALKPLVGGPSLGPSGSTFIHPLTGKPLDPSSPLALALAARERALASQTPSRSPTPVHSPDADRPGPLFVDVQTRDSERGPLASPAFSPRSPAWIPVPARREAEKPTREERKSPEDKKSMILSVLDTSLQRPAGLIVVHATSNGQEPNRLGAEEERPGTPELAPTPMQAAAVAEPMPSPRAQPPGSIPADPGPGQGSSEEEPELVFAVNLPPAQLSSSDEETREELARIGLVPPPEEFANGILLATPPPGPGPLPTTVPSPASGKPSSELPPAPESAADSGVEEADTRSSSDPHLETTSTISTVSSMSTLSSESGELTDTHTSFADGHTFLLEKPPVPPKPKLKSPLGKGPVTFRDPLLKQSSDSELMAQQHHATSTGLTSAAGPARPRYLFQRRSKLWGDPVESRGLPGPEDDKPTVISELSSRLQQLNKDTRSLGEEPVGGLGSLLDPAKKSPIAAARCAVVPSAGWLFSSLGELSTISAQRSPGGPGGGASYSVRPSGRYPVARRAPSPVKPASLERVEGLGAGVGGAGRPFGLTPPTILKSSSLSIPHEPKEVRFVVRSVSARSRSPSPSPLPSPSPGSGPSAGPRRPFQQKPLQLWSKFDVGDWLESIHLGEHRDRFEDHEIEGAHLPALTKEDFVELGVTRVGHRMNIERALRQLDGS

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
53	Phosphorylation	SLQDALNYGLFQPPS CHHHHHHCCCCCCCC	37.46	-
66	Ubiquitination	PSRGRAGKFLDEERL CCCCCCCCCCCHHHH	30.76	-
105	Ubiquitination	AQNLIDDKQFAKLHT HHCCCCHHHHHHHHH	11.31	-
122	Phosphorylation	NLKKFMDYVQLHSTD HHHHHHHHHHHCCHH	4.09	25403869
127	Phosphorylation	MDYVQLHSTDKVARL HHHHHHCCHHHHHHH	30.46	-
137	Ubiquitination	KVARLLDKGLDPNFH HHHHHHHCCCCCCCC	28.29	-
167	Ubiquitination	DNATDLLKVLRNGGA CCHHHHHHHHHHCCC	17.73	-
341	Phosphorylation	VIKTHKDSDVVPFRE HHHHCCCCCCCCCCC	2.19	30181290
349	Phosphorylation	DVVPFRETPSYAKRR CCCCCCCCCCHHHHC	7.46	22673903
351	Phosphorylation	VPFRETPSYAKRRRL CCCCCCCCHHHHCHH	4.70	22673903
352	Phosphorylation	PFRETPSYAKRRRLA CCCCCCCHHHHCHHC	24.66	22673903
362	Phosphorylation	RRRLAGPSGLASPRP HCHHCCCCCCCCCCC	2.35	30181290
366	Phosphorylation	AGPSGLASPRPLQRS CCCCCCCCCCCCCCC	16.69	30181290
373	Phosphorylation	SPRPLQRSASDINLK CCCCCCCCHHHCCCC	2.49	27097102
375	Phosphorylation	RPLQRSASDINLKGD CCCCCCHHHCCCCCC	24.76	29779826
387	Phosphorylation	KGDQPAASPGPTLRS CCCCCCCCCCCCHHH	50.47	29779826
391	Phosphorylation	PAASPGPTLRSLPHQ CCCCCCCCHHHHHHH	20.67	27097102
394	Phosphorylation	SPGPTLRSLPHQLLL CCCCCHHHHHHHHHH	5.80	29779826
434	Phosphorylation	RGGHSKISPSGPGGS CCCCCCCCCCCCCCC	26.49	26437020
436	Phosphorylation	GHSKISPSGPGGSGP CCCCCCCCCCCCCCC	21.44	26437020
441	Phosphorylation	SPSGPGGSGPAPGPG CCCCCCCCCCCCCCC	26.87	26437020
451	Phosphorylation	APGPGPASPAPPAPP CCCCCCCCCCCCCCC	33.25	22108457
467	Phosphorylation	RGPKRKLYSAVPGRK CCCCCCEEECCCCCE	3.84	-
468	Phosphorylation	GPKRKLYSAVPGRKF CCCCCEEECCCCCEE	38.33	-
482	Phosphorylation	FIAVKAHSPQGEGEI EEEEEECCCCCCCCC	47.50	30411139
550	Phosphorylation	TKRLFRHYTVGSYDS HHHHHHHCCCCCCCC	4.11	30181290
551	Phosphorylation	KRLFRHYTVGSYDSL HHHHHHCCCCCCCCC	4.90	30181290
554	Phosphorylation	FRHYTVGSYDSLTSH HHHCCCCCCCCCCCC	31.21	30181290
555	Phosphorylation	RHYTVGSYDSLTSHS HHCCCCCCCCCCCCC	11.20	30181290
557	Phosphorylation	YTVGSYDSLTSHSDY CCCCCCCCCCCCCCE	24.63	16452087
559	Phosphorylation	VGSYDSLTSHSDYVI CCCCCCCCCCCCEEE	66.63	30181290
564	Phosphorylation	SLTSHSDYVIDDKVA CCCCCCCEEECCEEE	6.91	-
685	Phosphorylation	PPPKRAPSTTLTLRS CCCCCCCCCEEEECC	43.72	23984901
686	Phosphorylation	PPKRAPSTTLTLRSK CCCCCCCCEEEECCC	22.48	23984901
687	Phosphorylation	PKRAPSTTLTLRSKS CCCCCCCEEEECCCC	7.54	23984901
694	Phosphorylation	TLTLRSKSMTAELEE EEEECCCCCCHHHHH	6.66	25403869
696	Phosphorylation	TLRSKSMTAELEELA EECCCCCCHHHHHHH	16.14	25575281
704	Phosphorylation	AELEELASIRRRKGE HHHHHHHHHHHHHCC	3.77	25575281
749	Phosphorylation	RPTSRRITPAEISSL CCCCCCCCHHHHHHH	38.54	22108457
779	Phosphorylation	LRKGIPRTKSVGEDE CCCCCCCCCCCCCHH	29.29	25575281
781	Phosphorylation	KGIPRTKSVGEDEKL CCCCCCCCCCCHHHH	29.83	29779826
790	Phosphorylation	GEDEKLASLLEGRFP CCHHHHHHHHCCCCC	50.79	30181290
799	Phosphorylation	LEGRFPRSTSMQDTV HCCCCCCCCCHHHHH	27.23	28432305
800	Phosphorylation	EGRFPRSTSMQDTVR CCCCCCCCCHHHHHH	12.53	28432305
801	Phosphorylation	GRFPRSTSMQDTVRE CCCCCCCCHHHHHHC	29.35	28432305
805	Phosphorylation	RSTSMQDTVREGRGI CCCCHHHHHHCCCCC	17.20	30181290
856	Phosphorylation	DTVRSSFKPGLEARL HHHHHHCCCCHHHHC	34.44	16452087
856	Ubiquitination	DTVRSSFKPGLEARL HHHHHHCCCCHHHHC	34.44	-
871	Phosphorylation	GAGAAGLYDSGTPLG CCCCCCCCCCCCCCC	9.66	-
891	Phosphorylation	ERQKRARSMIILQDS HHHHHHHEEEEECCC	50.34	30181290
898	Phosphorylation	SMIILQDSAPEVGDV EEEEECCCCCCCCCC	43.38	30181290
913	Phosphorylation	PRPAPAATPPERPKR CCCCCCCCCCCCCCC	38.69	-
929	Phosphorylation	PRPSGPDSPYANLGA CCCCCCCCCCCCCCH	27.07	22673903
931	Phosphorylation	PSGPDSPYANLGAFS CCCCCCCCCCCCHHH	39.87	22673903
945	Phosphorylation	SASLFAPSKPQRRKS HHHHCCCCCCCCCCC	3.53	-
946	Ubiquitination	ASLFAPSKPQRRKSP HHHCCCCCCCCCCCC	13.72	-
956	Ubiquitination	RRKSPLVKQLQVEDA CCCCCCHHHHCHHHH	45.85	-
966	Asymmetric dimethylarginine	QVEDAQERAALAVGS CHHHHHHHHHHHCCC	17.22	-
966	Methylation	QVEDAQERAALAVGS CHHHHHHHHHHHCCC	17.22	-
980	Phosphorylation	SPGPVGGSFAREPSP CCCCCCCCCCCCCCC	3.40	25403869
986	Phosphorylation	GSFAREPSPTHRGPR CCCCCCCCCCCCCCC	26.52	29779826
999	Phosphorylation	PRPGGLDYSSGEGLG CCCCCCCCCCCCCCC	46.44	-
1000	Phosphorylation	RPGGLDYSSGEGLGL CCCCCCCCCCCCCCC	65.32	-
1028	Phosphorylation	RLEERRRSTVFLSVG HHHHHHHCCEEEEEE	44.18	23984901
1029	Phosphorylation	LEERRRSTVFLSVGA HHHHHHCCEEEEEEE	10.97	23984901
1033	Phosphorylation	RRSTVFLSVGAIEGS HHCCEEEEEEECCCC	5.23	23984901
1040	Phosphorylation	SVGAIEGSPPSADLP EEEECCCCCCCCCCC	46.11	25575281
1074	Phosphorylation	GRDLLLPSPVSALKP CCCCCCCCCHHHCCC	15.13	22673903
1077	Phosphorylation	LLLPSPVSALKPLVG CCCCCCHHHCCCCCC	27.35	22673903
1123	Phosphorylation	ARERALASQTPSRSP HHHHHHHHCCCCCCC	47.11	25575281
1125	Phosphorylation	ERALASQTPSRSPTP HHHHHHCCCCCCCCC	37.96	25575281
1127	Phosphorylation	ALASQTPSRSPTPVH HHHHCCCCCCCCCCC	26.21	25575281
1129	Phosphorylation	ASQTPSRSPTPVHSP HHCCCCCCCCCCCCC	38.43	25575281
1131	Phosphorylation	QTPSRSPTPVHSPDA CCCCCCCCCCCCCCC	35.89	25403869
1135	Phosphorylation	RSPTPVHSPDADRPG CCCCCCCCCCCCCCC	7.85	29779826
1150	Phosphorylation	PLFVDVQTRDSERGP CEEEEEECCCCCCCC	23.34	25575281
1153	Phosphorylation	VDVQTRDSERGPLAS EEEECCCCCCCCCCC	21.60	25575281
1160	Phosphorylation	SERGPLASPAFSPRS CCCCCCCCCCCCCCC	10.92	27097102
1164	Phosphorylation	PLASPAFSPRSPAWI CCCCCCCCCCCCCCC	20.75	27097102
1167	Phosphorylation	SPAFSPRSPAWIPVP CCCCCCCCCCCCCCC	33.56	22108457
1235	Phosphorylation	AEEERPGTPELAPTP CCCCCCCCCCCCCCC	25.17	29779826
1241	Phosphorylation	GTPELAPTPMQAAAV CCCCCCCCCCCHHHH	54.82	26437020
1254	Phosphorylation	AVAEPMPSPRAQPPG HHCCCCCCCCCCCCC	28.44	25403869
1421	Phosphorylation	PPKPKLKSPLGKGPV CCCCCCCCCCCCCCC	7.13	22673903
1438	Phosphorylation	RDPLLKQSSDSELMA CCHHHCCCCCHHHHH	39.23	30181290
1439	Phosphorylation	DPLLKQSSDSELMAQ CHHHCCCCCHHHHHH	34.78	30181290
1441	Phosphorylation	LLKQSSDSELMAQQH HHCCCCCHHHHHHHH	35.15	30181290
1509	Phosphorylation	LQQLNKDTRSLGEEP HHHHCHHHHHCCCCC	12.51	23984901
1511	Phosphorylation	QLNKDTRSLGEEPVG HHCHHHHHCCCCCCC	56.68	27097102
1522	Phosphorylation	EPVGGLGSLLDPAKK CCCCCHHHHCCHHHC	22.31	23984901
1530	Phosphorylation	LLDPAKKSPIAAARC HCCHHHCCCCHHHHC	3.28	23984901
1548	Phosphorylation	PSAGWLFSSLGELST CCCHHHHHHHHHHHE	46.58	25403869
1549	Phosphorylation	SAGWLFSSLGELSTI CCHHHHHHHHHHHEE	6.96	25403869
1554	Phosphorylation	FSSLGELSTISAQRS HHHHHHHHEEEEECC	14.36	30181290
1555	Phosphorylation	SSLGELSTISAQRSP HHHHHHHEEEEECCC	39.86	30181290
1561	Phosphorylation	STISAQRSPGGPGGG HEEEEECCCCCCCCC	43.18	22673903
1587	Phosphorylation	PVARRAPSPVKPASL CCCCCCCCCCCCCCH	10.11	25403869
1593	Phosphorylation	PSPVKPASLERVEGL CCCCCCCCHHHCCCC	36.00	25403869
1621	Phosphorylation	TPPTILKSSSLSIPH CCCCCEECCCCCCCC	2.67	25575281
1622	Phosphorylation	PPTILKSSSLSIPHE CCCCEECCCCCCCCC	3.62	28432305
1623	Phosphorylation	PTILKSSSLSIPHEP CCCEECCCCCCCCCC	23.60	28432305
1625	Phosphorylation	ILKSSSLSIPHEPKE CEECCCCCCCCCCCE	6.31	25575281
1644	Phosphorylation	VRSVSARSRSPSPSP EEEEECCCCCCCCCC	14.47	27097102
1646	Phosphorylation	SVSARSRSPSPSPLP EEECCCCCCCCCCCC	15.62	27097102
1648	Phosphorylation	SARSRSPSPSPLPSP ECCCCCCCCCCCCCC	8.73	27097102
1650	Phosphorylation	RSRSPSPSPLPSPSP CCCCCCCCCCCCCCC	38.69	27097102
1654	Phosphorylation	PSPSPLPSPSPGSGP CCCCCCCCCCCCCCC	12.94	27097102
1656	Phosphorylation	PSPLPSPSPGSGPSA CCCCCCCCCCCCCCC	4.85	27097102
1662	Phosphorylation	PSPGSGPSAGPRRPF CCCCCCCCCCCCCCC	41.14	15358237

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
685	S	Phosphorylation	Kinase	PKACA	P17612	PSP
685	S	Phosphorylation	Kinase	PKACA	P27791	PSP
685	S	Phosphorylation	Kinase	CAMK2A	P11275	PSP
694	S	Phosphorylation	Kinase	CAMK2A	P11275	PSP
694	S	Phosphorylation	Kinase	CAMK2B	P08413	PSP
1511	S	Phosphorylation	Kinase	CAMK2A	P11275	PSP
1511	S	Phosphorylation	Kinase	CAMK2B	P08413	PSP

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of SHAN3_RAT !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of SHAN3_RAT !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
GRM5_RAT	Grm5	physical	10433269
SHAN3_RAT	Shank3	physical	23897824
RASH_HUMAN	HRAS	physical	28263956
RASK_HUMAN	KRAS	physical	28263956
RRAS_HUMAN	RRAS	physical	28263956
RASN_HUMAN	NRAS	physical	28263956
RAP1A_HUMAN	RAP1A	physical	28263956
RAP1B_HUMAN	RAP1B	physical	28263956

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.