RIR2B_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: RIR2B_HUMAN
• UniProt AC: Q7LG56
• Protein Name: Ribonucleoside-diphosphate reductase subunit M2 B
• Gene Name: RRM2B
• Organism: Homo sapiens (Human).
• Sequence Length: 351
• Subcellular Localization: Cytoplasm. Nucleus. Translocates from cytoplasm to nucleus in response to DNA damage.
• Protein Description: Plays a pivotal role in cell survival by repairing damaged DNA in a p53/TP53-dependent manner. Supplies deoxyribonucleotides for DNA repair in cells arrested at G1 or G2. Contains an iron-tyrosyl free radical center required for catalysis. Forms an active ribonucleotide reductase (RNR) complex with RRM1 which is expressed both in resting and proliferating cells in response to DNA damage..
• Protein Sequence: MGDPERPEAAGLDQDERSSSDTNESEIKSNEEPLLRKSSRRFVIFPIQYPDIWKMYKQAQASFWTAEEVDLSKDLPHWNKLKADEKYFISHILAFFAASDGIVNENLVERFSQEVQVPEARCFYGFQILIENVHSEMYSLLIDTYIRDPKKREFLFNAIETMPYVKKKADWALRWIADRKSTFGERVVAFAAVEGVFFSGSFAAIFWLKKRGLMPGLTFSNELISRDEGLHCDFACLMFQYLVNKPSEERVREIIVDAVKIEQEFLTEALPVGLIGMNCILMKQYIEFVADRLLVELGFSKVFQAENPFDFMENISLEGKTNFFEKRVSEYQRFAVMAETTDNVFTLDADF

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
17	Methylation	AGLDQDERSSSDTNE CCCCCCCCCCCCCCH	50.56	115492913
18	Phosphorylation	GLDQDERSSSDTNES CCCCCCCCCCCCCHH	31.95	25849741
19	Phosphorylation	LDQDERSSSDTNESE CCCCCCCCCCCCHHH	38.15	25159151
20	Phosphorylation	DQDERSSSDTNESEI CCCCCCCCCCCHHHH	50.10	29743597
22	Phosphorylation	DERSSSDTNESEIKS CCCCCCCCCHHHHHC	42.72	23927012
25	Phosphorylation	SSSDTNESEIKSNEE CCCCCCHHHHHCCCC	46.84	24275569
28 (in isoform 5)	Ubiquitination	-	43.08	21906983
28 (in isoform 1)	Ubiquitination	-	43.08	21906983
28	Ubiquitination	DTNESEIKSNEEPLL CCCHHHHHCCCCCHH	43.08	21906983
29	Phosphorylation	TNESEIKSNEEPLLR CCHHHHHCCCCCHHH	55.95	24275569
41 (in isoform 4)	Ubiquitination	-	31.93	21906983
72	Phosphorylation	TAEEVDLSKDLPHWN CHHHCCCCCCCCCHH	21.47	19015526
92 (in isoform 6)	Phosphorylation	-	2.07	28985074
97	Phosphorylation	SHILAFFAASDGIVN HHHHHHHHHHCCCCC	10.29	-
100	Ubiquitination	LAFFAASDGIVNENL HHHHHHHCCCCCHHH	46.22	22817900
101	Phosphorylation	AFFAASDGIVNENLV HHHHHHCCCCCHHHH	24.21	-
114 (in isoform 3)	Ubiquitination	-	33.45	21906983
114 (in isoform 2)	Ubiquitination	-	33.45	21906983
114	Ubiquitination	LVERFSQEVQVPEAR HHHHHHHHCCCCCHH	33.45	22817900
115	Ubiquitination	VERFSQEVQVPEARC HHHHHHHCCCCCHHH	5.57	22817900
116 (in isoform 2)	Ubiquitination	-	44.27	21906983
116	Ubiquitination	ERFSQEVQVPEARCF HHHHHHCCCCCHHHC	44.27	22817900
128	Ubiquitination	RCFYGFQILIENVHS HHCCCCHHHEECHHH	3.80	27667366
166 (in isoform 1)	Ubiquitination	-	42.59	21906983
166	Ubiquitination	IETMPYVKKKADWAL HHHCHHHHHHHHHHH	42.59	22817900
167	Ubiquitination	ETMPYVKKKADWALR HHCHHHHHHHHHHHH	43.05	22817900
168	Ubiquitination	TMPYVKKKADWALRW HCHHHHHHHHHHHHH	45.95	22817900
168 (in isoform 1)	Ubiquitination	-	45.95	21906983
180	Ubiquitination	LRWIADRKSTFGERV HHHHHHCCCCCCHHH	55.16	27667366
218	Phosphorylation	RGLMPGLTFSNELIS CCCCCCCEECHHHHH	30.82	20068231
225	Phosphorylation	TFSNELISRDEGLHC EECHHHHHCCCCCCC	46.43	24719451
238	Ubiquitination	HCDFACLMFQYLVNK CCHHHHHHHHHHHCC	1.66	22817900
239	Ubiquitination	CDFACLMFQYLVNKP CHHHHHHHHHHHCCC	2.52	22817900
240	Ubiquitination	DFACLMFQYLVNKPS HHHHHHHHHHHCCCC	19.38	22817900
252	Ubiquitination	KPSEERVREIIVDAV CCCHHHHHHHHHHHH	35.30	27667366
268	Ubiquitination	IEQEFLTEALPVGLI HCHHHHHHHCCCCHH	50.81	22817900
274 (in isoform 2)	Ubiquitination	-	2.89	21906983
274	Ubiquitination	TEALPVGLIGMNCIL HHHCCCCHHCHHHHH	2.89	21963094
320	Ubiquitination	ENISLEGKTNFFEKR HHCCCCCCCCHHHHH	30.81	22817900
326	Acetylation	GKTNFFEKRVSEYQR CCCCHHHHHHHHHHH	53.60	11372325
326 (in isoform 1)	Ubiquitination	-	53.60	21906983
326	Ubiquitination	GKTNFFEKRVSEYQR CCCCHHHHHHHHHHH	53.60	21963094
329	Phosphorylation	NFFEKRVSEYQRFAV CHHHHHHHHHHHHHH	34.32	28857561
331	Phosphorylation	FEKRVSEYQRFAVMA HHHHHHHHHHHHHEE	9.47	-
392	Ubiquitination	------------------------------------------------ ------------------------------------------------		22817900
398	Ubiquitination	------------------------------------------------------ ------------------------------------------------------		21963094
401	Phosphorylation	--------------------------------------------------------- ---------------------------------------------------------		27251275

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
72	S	Phosphorylation	Kinase	ATM	Q13315	PSP
-	K	Ubiquitination	E3 ubiquitin ligase	MDM2	Q00987	PMID:19015526

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of RIR2B_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of RIR2B_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
P53_HUMAN	TP53	physical	12615712
MDM2_HUMAN	MDM2	physical	19015526
ATM_HUMAN	ATM	physical	19015526
RIR2B_HUMAN	RRM2B	physical	25416956
RIR2_HUMAN	RRM2	physical	26186194
SHIP2_HUMAN	INPPL1	physical	26186194
RIR2_HUMAN	RRM2	physical	28514442
SHIP2_HUMAN	INPPL1	physical	28514442
TPM2_HUMAN	TPM2	physical	28514442
JIP4_HUMAN	SPAG9	physical	28514442

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• 612075: Mitochondrial DNA depletion syndrome 8A (MTDPS8A)
• 612075: Mitochondrial DNA depletion syndrome 8B (MTDPS8B)
• 613077: Progressive external ophthalmoplegia with mitochondrial DNA deletions, autosomal dominant, 5 (PEOA5)
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• DB00242: Cladribine