RIMS1_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: RIMS1_MOUSE
• UniProt AC: Q99NE5
• Protein Name: Regulating synaptic membrane exocytosis protein 1
• Gene Name: Rims1
• Organism: Mus musculus (Mouse).
• Sequence Length: 1463
• Subcellular Localization: Cell membrane; Peripheral membrane protein. Cell junction, synapse. Cell junction, synapse, presynaptic cell membrane; Peripheral membrane protein.
• Protein Description: Rab effector involved in exocytosis. [PubMed: 11797009 May act as scaffold protein that regulates neurotransmitter release at the active zone. Essential for maintaining normal probability of neurotransmitter release and for regulating release during short-term synaptic plasticity]
• Protein Sequence: MSSAVGPRGPRPPTVPPPMQELPDLSHLTEEERNIIMAVMDRQKEEEEKEEAMLKCVVRDMAKPAACKTPRNAESQPHQPPLNIFRCVCVPRKPSSEEGGPDRNWRLHQQFESYKEQVRKIGEEARRYQGEHKDDAPTCGICHKTKFADGCGHLCSYCRTKFCARCGGRVSLRSNNEDKVVMWVCNLCRKQQEILTKSGAWFFGSGPQQPSQDGTLSDTATGAGSEVPREKKARLQERSRSQTPLSTAAVSSQDTASHGAPLDRNKGAEPSQQALGPEQKQASRSRSEPPRERKKAPGLSEQNGKGGQKSERKRVPKSVVQPGEGTADERERKERRETRRLEKGRSQDYPDRLEKREDGRVAEDEKQRKEEEGVSTPEYTSCEDVELESESVSEKGDLDYWLDPATWHSRETSPISSHPVTWQPSKEGDRLIGRVILNKRTTMPKESGALLGLKVVGGKMTDLGRLGAFITKVKKGSLADVVGHLRAGDEVLEWNGKPLPGATNEEVYNIILESKSEPQVEIIVSRPIGDIPRIPESSHPPLESSSSSFESQKMERPSISVISPTSPGALKDAPQVLPGQLSVKLWYDKVGHQLIVNVLQATDLPPRVDGRPRNPYVKMYFLPDRSDKSKRRTKTVKKLLEPKWNQTFVYSHVHRRDFRERMLEITVWDQPRVQDEESEFLGEILIELETALLDDEPHWYKLQTHDESSLPLPQPSPFMPRRHIHGESSSKKLQRSQRISDSDISDYEVDDGIGVVPPVGYRASARESKATTLTVPEQQRTTHHRSRSVSPHRGDDQGRPRSRLPNVPLQRSLDEIHPTRRSRSPTRHHDASRSLADHRSRHAESQYSSEPDSELLMLPRAKRGRSAECLHMTSELQPSLDRARSASTNCLRPDTSLHSPERERGRWSPSLARRRPASPRIQIQHASPENDRHSRKSERSSIQKQSRKGTASDADRVLPPCLSRRGYAIPRATDQPVIRGKHTTRSRSSEHSSIRTLCSMHHLAPGGSAPPSPLLTRTHRQGSPTQSPPADTSFGSRRGRQLPQVPVRSGSIEQASLVVEERTRQMKMKVHRFKQTTGSGSSQELDHEQYSKYNIHKDQYRSCDNASAKSSDSDVSDVSAISRASSTSRLSSTSFMSEQSERPRGRISSFTPKMQGRRMGTSGRAIIKSTSVSGEIYTLEHNDGSQSDTAVGTVGAGGKKRRSSLSAKVVAIVSRRSRSTSQLSQTESGHKKLKSTIQRSTETGMAAEMRKMVRQPSRESTDGSINSYSSEGNLIFPGVRVGPDSQFSDFLDGLGPAQLVGRQTLATPAMGDIQIGMEDKKGQLEVEVIRARSLTQKPGSKSTPAPYVKVYLLENGACIAKKKTRIARKTLDPLYQQSLVFDESPQGKVLQVIVWGDYGRMDHKCFMGVAQILLEELDLSSMVIGWYKLFPPSSLVDPTLTPLTRRASQSSLESSSGPPCIRS

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
44	Methylation	MAVMDRQKEEEEKEE HHHHHHHHHHHHHHH	68.90	-
55	Methylation	EKEEAMLKCVVRDMA HHHHHHHHHHHHHCC	16.34	-
95	Phosphorylation	VCVPRKPSSEEGGPD EEEECCCCCCCCCCC	54.11	25521595
96	Phosphorylation	CVPRKPSSEEGGPDR EEECCCCCCCCCCCC	48.47	25521595
113	Phosphorylation	RLHQQFESYKEQVRK HHHHHHHHHHHHHHH	42.67	29899451
114	Phosphorylation	LHQQFESYKEQVRKI HHHHHHHHHHHHHHH	16.02	25521595
205	Phosphorylation	SGAWFFGSGPQQPSQ CCCCCCCCCCCCCCC	41.99	22807455
211	Phosphorylation	GSGPQQPSQDGTLSD CCCCCCCCCCCCCCC	36.24	26804993
215	Phosphorylation	QQPSQDGTLSDTATG CCCCCCCCCCCCCCC	31.23	21183079
217	Phosphorylation	PSQDGTLSDTATGAG CCCCCCCCCCCCCCC	32.52	19060867
219	Phosphorylation	QDGTLSDTATGAGSE CCCCCCCCCCCCCCC	23.81	26804993
221	Phosphorylation	GTLSDTATGAGSEVP CCCCCCCCCCCCCCC	29.65	26804993
225	Phosphorylation	DTATGAGSEVPREKK CCCCCCCCCCCHHHH	34.58	20415495
239	Phosphorylation	KARLQERSRSQTPLS HHHHHHHHHCCCCCC	35.22	25521595
241	Phosphorylation	RLQERSRSQTPLSTA HHHHHHHCCCCCCHH	39.39	19060867
243	Phosphorylation	QERSRSQTPLSTAAV HHHHHCCCCCCHHHC	27.69	25521595
246	Phosphorylation	SRSQTPLSTAAVSSQ HHCCCCCCHHHCCCC	19.73	29899451
247	Phosphorylation	RSQTPLSTAAVSSQD HCCCCCCHHHCCCCC	26.73	29899451
251	Phosphorylation	PLSTAAVSSQDTASH CCCHHHCCCCCHHHC	19.97	29899451
252	Phosphorylation	LSTAAVSSQDTASHG CCHHHCCCCCHHHCC	26.02	29899451
287	Phosphorylation	KQASRSRSEPPRERK HHHHHHCCCCCHHHC	56.56	29899451
310	Phosphorylation	NGKGGQKSERKRVPK CCCCCCCCCCCCCCC	34.44	21183079
326	Phosphorylation	VVQPGEGTADERERK CCCCCCCCCCHHHHH	27.00	29899451
346	Phosphorylation	RRLEKGRSQDYPDRL HHHHHHCCCCCHHHH	35.75	25521595
349	Phosphorylation	EKGRSQDYPDRLEKR HHHCCCCCHHHHHHC	10.21	29899451
379 (in isoform 2)	Phosphorylation	-	12.73	-
379 (in isoform 7)	Phosphorylation	-	12.73	-
406	Phosphorylation	DYWLDPATWHSRETS CEEECHHHCCCCCCC	29.15	21082442
409	Phosphorylation	LDPATWHSRETSPIS ECHHHCCCCCCCCCC	24.31	19060867
412	Phosphorylation	ATWHSRETSPISSHP HHCCCCCCCCCCCCC	38.40	30372032
413	Phosphorylation	TWHSRETSPISSHPV HCCCCCCCCCCCCCC	18.58	25521595
413 (in isoform 7)	Phosphorylation	-	18.58	21183079
416	Phosphorylation	SRETSPISSHPVTWQ CCCCCCCCCCCCCCC	26.44	23335269
417	Phosphorylation	RETSPISSHPVTWQP CCCCCCCCCCCCCCC	32.31	23335269
421	Phosphorylation	PISSHPVTWQPSKEG CCCCCCCCCCCCCCC	23.88	26804993
438 (in isoform 7)	Phosphorylation	-	22.19	25521595
439 (in isoform 7)	Phosphorylation	-	47.91	25521595
443 (in isoform 7)	Phosphorylation	-	4.39	25521595
447 (in isoform 7)	Phosphorylation	-	35.18	25521595
508 (in isoform 7)	Phosphorylation	-	12.15	29899451
514 (in isoform 7)	Phosphorylation	-	37.73	21183079
516 (in isoform 7)	Phosphorylation	-	64.88	21183079
517 (in isoform 7)	Phosphorylation	-	43.41	21183079
526 (in isoform 7)	Phosphorylation	-	18.89	21183079
537	Phosphorylation	DIPRIPESSHPPLES CCCCCCCCCCCCCCC	28.70	20415495
538	Phosphorylation	IPRIPESSHPPLESS CCCCCCCCCCCCCCC	38.05	29899451
544	Phosphorylation	SSHPPLESSSSSFES CCCCCCCCCCCHHHH	43.11	20415495
545	Phosphorylation	SHPPLESSSSSFESQ CCCCCCCCCCHHHHH	25.51	22807455
546	Phosphorylation	HPPLESSSSSFESQK CCCCCCCCCHHHHHC	39.00	21183079
547	Phosphorylation	PPLESSSSSFESQKM CCCCCCCCHHHHHCC	40.78	19060867
548	Phosphorylation	PLESSSSSFESQKME CCCCCCCHHHHHCCC	34.67	30372032
551	Phosphorylation	SSSSSFESQKMERPS CCCCHHHHHCCCCCC	32.84	20415495
558	Phosphorylation	SQKMERPSISVISPT HHCCCCCCEEEECCC	33.44	24925903
560	Phosphorylation	KMERPSISVISPTSP CCCCCCEEEECCCCC	20.10	22324799
563	Phosphorylation	RPSISVISPTSPGAL CCCEEEECCCCCCHH	21.70	25521595
565	Phosphorylation	SISVISPTSPGALKD CEEEECCCCCCHHCC	40.54	25521595
566	Phosphorylation	ISVISPTSPGALKDA EEEECCCCCCHHCCC	25.40	25521595
716	Phosphorylation	SLPLPQPSPFMPRRH CCCCCCCCCCCCCCC	26.06	25521595
728	Phosphorylation	RRHIHGESSSKKLQR CCCCCCCCCHHHHHH	44.50	27742792
729	Phosphorylation	RHIHGESSSKKLQRS CCCCCCCCHHHHHHH	41.70	27742792
730	Phosphorylation	HIHGESSSKKLQRSQ CCCCCCCHHHHHHHH	42.99	27742792
736	Phosphorylation	SSKKLQRSQRISDSD CHHHHHHHHCCCCCC	15.73	21183079
740	Phosphorylation	LQRSQRISDSDISDY HHHHHCCCCCCCCCC	33.00	18388127
742	Phosphorylation	RSQRISDSDISDYEV HHHCCCCCCCCCCCC	30.01	18388127
745	Phosphorylation	RISDSDISDYEVDDG CCCCCCCCCCCCCCC	38.85	18388127
747	Phosphorylation	SDSDISDYEVDDGIG CCCCCCCCCCCCCCC	15.86	29899451
786	Phosphorylation	QRTTHHRSRSVSPHR HCCCCCCCCCCCCCC	25.26	29899451
788	Phosphorylation	TTHHRSRSVSPHRGD CCCCCCCCCCCCCCC	28.35	29899451
790	Phosphorylation	HHRSRSVSPHRGDDQ CCCCCCCCCCCCCCC	19.08	29899451
802	Phosphorylation	DDQGRPRSRLPNVPL CCCCCCCCCCCCCCC	40.63	29899451
812	Phosphorylation	PNVPLQRSLDEIHPT CCCCCCCCHHHCCCC	27.49	25521595
819	Phosphorylation	SLDEIHPTRRSRSPT CHHHCCCCCCCCCCC	25.26	29176673
822	Phosphorylation	EIHPTRRSRSPTRHH HCCCCCCCCCCCCCH	33.54	29176673
834	Phosphorylation	RHHDASRSLADHRSR CCHHHHHHHHHHHHH	25.97	29899451
848 (in isoform 6)	Phosphorylation	-	22.16	29899451
849	Phosphorylation	HAESQYSSEPDSELL HHHHHHCCCCCHHHC	48.49	29899451
849 (in isoform 6)	Phosphorylation	-	48.49	29899451
866	Phosphorylation	PRAKRGRSAECLHMT CCHHCCCCHHHHHHH	31.10	22324799
873	Phosphorylation	SAECLHMTSELQPSL CHHHHHHHCCCCHHH	14.33	19060867
874	Phosphorylation	AECLHMTSELQPSLD HHHHHHHCCCCHHHH	28.83	22324799
879	Phosphorylation	MTSELQPSLDRARSA HHCCCCHHHHHHHHH	29.91	29899451
887	Phosphorylation	LDRARSASTNCLRPD HHHHHHHCCCCCCCC	22.77	21183079
895	Phosphorylation	TNCLRPDTSLHSPER CCCCCCCCCCCCCHH	35.55	21183079
896	Phosphorylation	NCLRPDTSLHSPERE CCCCCCCCCCCCHHH	31.31	22807455
899	Phosphorylation	RPDTSLHSPERERGR CCCCCCCCCHHHCCC	33.48	19060867
908	Phosphorylation	ERERGRWSPSLARRR HHHCCCCCHHHHHCC	11.84	30372032
927	Phosphorylation	RIQIQHASPENDRHS CCEEEECCCCCCCCC	30.53	25521595
934 (in isoform 4)	Phosphorylation	-	42.15	29899451
936 (in isoform 4)	Phosphorylation	-	60.73	29899451
986	Phosphorylation	RGKHTTRSRSSEHSS CCCCCCCCCCCCHHH	34.13	29899451
988	Phosphorylation	KHTTRSRSSEHSSIR CCCCCCCCCCHHHHH	41.13	29899451
992	Phosphorylation	RSRSSEHSSIRTLCS CCCCCCHHHHHHHHH	24.33	29899451
993	Phosphorylation	SRSSEHSSIRTLCSM CCCCCHHHHHHHHHH	20.28	29899451
999	Phosphorylation	SSIRTLCSMHHLAPG HHHHHHHHHCCCCCC	25.02	28059163
1008	Phosphorylation	HHLAPGGSAPPSPLL CCCCCCCCCCCCCCC	43.44	-
1018	Phosphorylation	PSPLLTRTHRQGSPT CCCCCCCCCCCCCCC	19.04	29899451
1023	Phosphorylation	TRTHRQGSPTQSPPA CCCCCCCCCCCCCCC	19.21	25521595
1025	Phosphorylation	THRQGSPTQSPPADT CCCCCCCCCCCCCCC	43.29	25521595
1026 (in isoform 7)	Phosphorylation	-	58.27	29899451
1027	Phosphorylation	RQGSPTQSPPADTSF CCCCCCCCCCCCCCC	35.02	25521595
1027 (in isoform 7)	Phosphorylation	-	35.02	29899451
1049	Phosphorylation	LPQVPVRSGSIEQAS CCCCCCCCCCHHHHH	36.81	25521595
1051	Phosphorylation	QVPVRSGSIEQASLV CCCCCCCCHHHHHHH	24.97	25521595
1056	Phosphorylation	SGSIEQASLVVEERT CCCHHHHHHHHHHHH	22.76	29899451
1076	Phosphorylation	KVHRFKQTTGSGSSQ EEEEECCCCCCCCCC	33.44	22324799
1077	Phosphorylation	VHRFKQTTGSGSSQE EEEECCCCCCCCCCC	27.19	22324799
1079	Phosphorylation	RFKQTTGSGSSQELD EECCCCCCCCCCCCC	33.15	22324799
1081	Phosphorylation	KQTTGSGSSQELDHE CCCCCCCCCCCCCHH	30.46	22324799
1082	Phosphorylation	QTTGSGSSQELDHEQ CCCCCCCCCCCCHHH	31.06	29899451
1090	Phosphorylation	QELDHEQYSKYNIHK CCCCHHHHHHCCCCH	12.26	22807455
1091	Phosphorylation	ELDHEQYSKYNIHKD CCCHHHHHHCCCCHH	28.49	22324799
1100	Phosphorylation	YNIHKDQYRSCDNAS CCCCHHHHHCCCCCC	18.03	29899451
1102	Phosphorylation	IHKDQYRSCDNASAK CCHHHHHCCCCCCCC	22.38	29899451
1107	Phosphorylation	YRSCDNASAKSSDSD HHCCCCCCCCCCCCC	42.35	20415495
1110	Phosphorylation	CDNASAKSSDSDVSD CCCCCCCCCCCCHHH	38.81	25521595
1111	Phosphorylation	DNASAKSSDSDVSDV CCCCCCCCCCCHHHH	40.19	25521595
1113	Phosphorylation	ASAKSSDSDVSDVSA CCCCCCCCCHHHHHH	41.69	25521595
1116	Phosphorylation	KSSDSDVSDVSAISR CCCCCCHHHHHHHHH	36.97	22807455
1119	Phosphorylation	DSDVSDVSAISRASS CCCHHHHHHHHHHHC	25.52	20415495
1122	Phosphorylation	VSDVSAISRASSTSR HHHHHHHHHHHCCCC	22.85	20415495
1131	Phosphorylation	ASSTSRLSSTSFMSE HHCCCCCCCCCCCCC	30.38	29899451
1132	Phosphorylation	SSTSRLSSTSFMSEQ HCCCCCCCCCCCCCC	32.44	29899451
1133	Phosphorylation	STSRLSSTSFMSEQS CCCCCCCCCCCCCCC	23.70	23567750
1149	Phosphorylation	RPRGRISSFTPKMQG CCCCCCCCCCHHHCC	30.67	29899451
1169	Phosphorylation	SGRAIIKSTSVSGEI CCCEEEEEEEECCEE	18.59	19060867
1170	Phosphorylation	GRAIIKSTSVSGEIY CCEEEEEEEECCEEE	28.31	29899451
1171	Phosphorylation	RAIIKSTSVSGEIYT CEEEEEEEECCEEEE	22.75	19060867
1173	Phosphorylation	IIKSTSVSGEIYTLE EEEEEEECCEEEEEE	30.40	22807455
1177	Phosphorylation	TSVSGEIYTLEHNDG EEECCEEEEEECCCC	10.85	29899451
1178	Phosphorylation	SVSGEIYTLEHNDGS EECCEEEEEECCCCC	31.19	29899451
1185	Phosphorylation	TLEHNDGSQSDTAVG EEECCCCCCCCCEEE	29.40	22807455
1187	Phosphorylation	EHNDGSQSDTAVGTV ECCCCCCCCCEEEEE	38.33	19060867
1189	Phosphorylation	NDGSQSDTAVGTVGA CCCCCCCCEEEEECC	28.61	22807455
1193	Phosphorylation	QSDTAVGTVGAGGKK CCCCEEEEECCCCCC	14.79	29899451
1203	Phosphorylation	AGGKKRRSSLSAKVV CCCCCCCCCCHHHHH	39.40	29899451
1204	Phosphorylation	GGKKRRSSLSAKVVA CCCCCCCCCHHHHHE	25.24	29899451
1206	Phosphorylation	KKRRSSLSAKVVAIV CCCCCCCHHHHHEEE	28.17	-
1214	Phosphorylation	AKVVAIVSRRSRSTS HHHHEEEECCCCCHH	18.83	-
1217	Phosphorylation	VAIVSRRSRSTSQLS HEEEECCCCCHHHCC	29.49	29899451
1219	Phosphorylation	IVSRRSRSTSQLSQT EEECCCCCHHHCCCC	33.39	29899451
1224	Phosphorylation	SRSTSQLSQTESGHK CCCHHHCCCCHHHHH	27.67	29899451
1235	Phosphorylation	SGHKKLKSTIQRSTE HHHHHHHHHHHHHHH	40.97	29899451
1236	Phosphorylation	GHKKLKSTIQRSTET HHHHHHHHHHHHHHH	21.39	29899451
1240	Phosphorylation	LKSTIQRSTETGMAA HHHHHHHHHHHHHHH	17.77	25521595
1241	Phosphorylation	KSTIQRSTETGMAAE HHHHHHHHHHHHHHH	39.49	19060867
1257	Phosphorylation	RKMVRQPSRESTDGS HHHHCCCCCCCCCCC	40.10	25521595
1260	Phosphorylation	VRQPSRESTDGSINS HCCCCCCCCCCCCCC	30.59	24925903
1261	Phosphorylation	RQPSRESTDGSINSY CCCCCCCCCCCCCCC	39.36	25521595
1264	Phosphorylation	SRESTDGSINSYSSE CCCCCCCCCCCCCCC	22.77	29899451
1267	Phosphorylation	STDGSINSYSSEGNL CCCCCCCCCCCCCCE	25.40	29899451
1268	Phosphorylation	TDGSINSYSSEGNLI CCCCCCCCCCCCCEE	16.24	29899451
1269	Phosphorylation	DGSINSYSSEGNLIF CCCCCCCCCCCCEEC	23.02	29899451
1270	Phosphorylation	GSINSYSSEGNLIFP CCCCCCCCCCCEECC	41.09	24925903
1384	Phosphorylation	QSLVFDESPQGKVLQ CCCEECCCCCCCEEE	25.11	29899451
1444	Phosphorylation	DPTLTPLTRRASQSS CCCCCHHHHHHHHHH	21.22	-
1448	Phosphorylation	TPLTRRASQSSLESS CHHHHHHHHHHHHCC	28.87	25521595
1450	Phosphorylation	LTRRASQSSLESSSG HHHHHHHHHHHCCCC	34.10	18388127
1451	Phosphorylation	TRRASQSSLESSSGP HHHHHHHHHHCCCCC	28.63	25521595
1454	Phosphorylation	ASQSSLESSSGPPCI HHHHHHHCCCCCCCC	34.79	22324799
1455	Phosphorylation	SQSSLESSSGPPCIR HHHHHHCCCCCCCCC	29.91	22324799
1456	Phosphorylation	QSSLESSSGPPCIRS HHHHHCCCCCCCCCC	65.68	22324799
1463	Phosphorylation	SGPPCIRS------- CCCCCCCC-------	25.35	29899451

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
413	S	Phosphorylation	Kinase	PRKACA	P17612	GPS

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of RIMS1_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of RIMS1_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
RAB3A_MOUSE	Rab3a	physical	11056535
RPGF4_MOUSE	Rapgef4	physical	11056535
RAB3A_MOUSE	Rab3a	physical	12578829
RAB3B_MOUSE	Rab3b	physical	12578829
RAB3C_MOUSE	Rab3c	physical	12578829
RAB3D_MOUSE	Rab3d	physical	12578829
RAB10_MOUSE	Rab10	physical	12578829
RAB26_MOUSE	Rab26	physical	12578829
RAB37_MOUSE	Rab37	physical	12578829

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"Qualitative and quantitative analyses of protein phosphorylation innaive and stimulated mouse synaptosomal preparations.";
Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F.,Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D.,Gerrits B., Panse C., Schlapbach R., Mansuy I.M.;
Mol. Cell. Proteomics 6:283-293(2007).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-716; SER-812; SER-1023;THR-1025 AND SER-1448, AND MASS SPECTROMETRY.
PubMed: 17114649

"Comprehensive identification of phosphorylation sites in postsynapticdensity preparations.";
Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R.,Burlingame A.L.;
Mol. Cell. Proteomics 5:914-922(2006).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-346; SER-812; THR-1025;SER-1027 AND SER-1451, AND MASS SPECTROMETRY.
PubMed: 16452087