dbPTM

RPGF4_MOUSE - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	RPGF4_MOUSE
UniProt AC	Q9EQZ6
Protein Name	Rap guanine nucleotide exchange factor 4
Gene Name	Rapgef4
Organism	Mus musculus (Mouse).
Sequence Length	1011
Subcellular Localization	Cytoplasm. Membrane Peripheral membrane protein.
Protein Description	Guanine nucleotide exchange factor (GEF) for RAP1A, RAP1B and RAP2A small GTPases that is activated by binding cAMP. Seems not to activate RAB3A. Involved in cAMP-dependent, PKA-independent exocytosis through interaction with RIMS2..
Protein Sequence	MVAAHAAHSQSSAEWIACLDKRPLERSSEDVDIIFTRLKGVKAFEKFHPNLLRQICLCGYYENLEKGITLFRQGDIGTNWYAVLAGSLDVKVSETSSHQDAVTICTLGIGTAFGESILDNTPRHATIVTRESSELLRIEQEDFKALWEKYRQYMAGLLAPPYGVMETGSNNDRIPDKENTPLIEPHVPLRPAHTITKVPSEKILRAGKILRIAILSRAPHMIRDRKYHLKTYRQCCVGTELVDWMIQQTSCVHSRTQAVGMWQVLLEDGVLNHVDQERHFQDKYLFYRFLDDEREDAPLPTEEEKKECDEELQDTMLLLSQMGPDAHMRMILRKPPGQRTVDDLEIIYDELLHIKALSHLSTTVKRELAGVLIFESHAKGGTVLFNQGEEGTSWYIILKGSVNVVIYGKGVVCTLHEGDDFGKLALVNDAPRAASIVLREDNCHFLRVDKEDFNRILRDVEANTVRLKEHDQDVLVLEKVPAGNRAANQGNSQPQQKYTVMSGTPEKILEHFLETIRLEPSLNEATDSVLNDFVMMHCVFMPNTQLCPALVAHYHAQPSQGTEQERMDYALNNKRRVIRLVLQWAAMYGDLLQEDDVAMAFLEEFYVSVSDDARMMAAFKEQLPELEKIVKQISEDAKAPQKKHKVLLQQFNTGDERAQKRQPIRGSDEVLFKVYCIDHTYTTIRVPVAASVKEVISAVADKLGSGEGLIIVKMNSGGEKVVLKSNDVSVFTTLTINGRLFACPREQFDSLTPLPEQEGPTTGTVGTFELMSSKDLAYQMTTYDWELFNCVHELELIYHTFGRHNFKKTTANLDLFLRRFNEIQFWVVTEVCLCSQLSKRVQLLKKFIKIAAHCKEYKNLNSFFAIVMGLSNVAVSRLALTWEKLPSKFKKFYAEFESLMDPSRNHRAYRLTAAKLEPPLIPFMPLLIKDMTFTHEGNKTFIDNLVNFEKMRMIANTARTVRYYRSQPFNPDAAQANKNHQDVRSYVRQLNVIDNQRTLSQMSHRLEPRRP

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
153 (in isoform 3)	Phosphorylation	-	4.67	29895711
167 (in isoform 3)	Phosphorylation	-	28.02	29895711
169 (in isoform 3)	Phosphorylation	-	39.62	29895711
182 (in isoform 3)	Phosphorylation	-	6.97	29895711
184 (in isoform 3)	Ubiquitination	-	36.72	-
202	Ubiquitination	ITKVPSEKILRAGKI EEECCCHHHHHHCCH	51.91	-
208	Acetylation	EKILRAGKILRIAIL HHHHHHCCHHHHHHH	37.27	22902405
283	Ubiquitination	QERHFQDKYLFYRFL CHHHCCCCCHHHCCC	32.82	-
320	Phosphorylation	QDTMLLLSQMGPDAH HHHHHHHHHHCCCHH	20.35	29233185
348	Phosphorylation	VDDLEIIYDELLHIK CCHHHHHHHHHHHHH	14.71	22817900
358	Phosphorylation	LLHIKALSHLSTTVK HHHHHHHHCCCHHHH	27.52	25367039
361	Phosphorylation	IKALSHLSTTVKREL HHHHHCCCHHHHHHH	19.33	25367039
362	Phosphorylation	KALSHLSTTVKRELA HHHHCCCHHHHHHHC	41.80	25367039
363	Phosphorylation	ALSHLSTTVKRELAG HHHCCCHHHHHHHCC	22.21	25367039
376	Phosphorylation	AGVLIFESHAKGGTV CCEEEEEECCCCCEE	20.28	25367039
631	Ubiquitination	PELEKIVKQISEDAK HHHHHHHHHHHHHCC	45.44	-
645	Ubiquitination	KAPQKKHKVLLQQFN CCCHHHHHHHHHHCC	43.38	-
653	Phosphorylation	VLLQQFNTGDERAQK HHHHHCCCCCHHHHH	47.50	22817900
680	Phosphorylation	KVYCIDHTYTTIRVP EEEEEECCEEEEECC	20.86	22817900
681	Phosphorylation	VYCIDHTYTTIRVPV EEEEECCEEEEECCC	9.85	22817900
682	Phosphorylation	YCIDHTYTTIRVPVA EEEECCEEEEECCCC	19.53	22817900
683	Phosphorylation	CIDHTYTTIRVPVAA EEECCEEEEECCCCC	9.07	22817900
693	Ubiquitination	VPVAASVKEVISAVA CCCCCCHHHHHHHHH	43.71	-
857	Phosphorylation	IAAHCKEYKNLNSFF HHHHHHHCCCHHHHH	7.23	-
915	Ubiquitination	AYRLTAAKLEPPLIP HHHHHHHHCCCCCCC	51.42	-
950	Ubiquitination	DNLVNFEKMRMIANT HHHCCHHHHHHHHHH	27.89	-
978	Ubiquitination	PDAAQANKNHQDVRS CCHHHHCCCHHHHHH	59.84	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
Oops, there are no upstream regulatory protein records of RPGF4_MOUSE !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of RPGF4_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of RPGF4_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference	Domain-Domain Interactions
Oops, there are no PPI records of RPGF4_MOUSE !!

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of RPGF4_MOUSE

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Large-scale identification and evolution indexing of tyrosinephosphorylation sites from murine brain."; Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.; J. Proteome Res. 7:311-318(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-681, AND MASSSPECTROMETRY.	18034455 [Abstract]