PYRF_YEAST - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: PYRF_YEAST
• UniProt AC: P03962
• Protein Name: Orotidine 5'-phosphate decarboxylase
• Gene Name: URA3
• Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
• Sequence Length: 267
• Protein Sequence: MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELLELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQYSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLATGEYTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDDVVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Acetylation	------MSKATYKER ------CCCHHHHHH	30.88	22814378
5	Phosphorylation	---MSKATYKERAAT ---CCCHHHHHHHCC	38.57	27017623
12	Phosphorylation	TYKERAATHPSPVAA HHHHHHCCCCCHHHH	33.08	23749301
15	Phosphorylation	ERAATHPSPVAAKLF HHHCCCCCHHHHHHH	25.13	23749301
20	Ubiquitination	HPSPVAAKLFNIMHE CCCHHHHHHHHHHHH	43.82	23749301
28	Ubiquitination	LFNIMHEKQTNLCAS HHHHHHHHCCCCCHH	47.98	23749301
42	Ubiquitination	SLDVRTTKELLELVE HCCCCCHHHHHHHHH	45.83	23749301
54	Ubiquitination	LVEALGPKICLLKTH HHHHHCHHEEEEEHH	44.23	23749301
59	Ubiquitination	GPKICLLKTHVDILT CHHEEEEEHHHHHHH	24.52	23749301
75	Ubiquitination	FSMEGTVKPLKALSA CCCCCCCCCHHHHHH	44.46	22817900
78	Ubiquitination	EGTVKPLKALSAKYN CCCCCCHHHHHHHCC	56.67	23749301
83	Ubiquitination	PLKALSAKYNFLLFE CHHHHHHHCCEEEEC	37.03	22817900
93	Ubiquitination	FLLFEDRKFADIGNT EEEECCCCCCCCCCE	58.17	23749301
102	Ubiquitination	ADIGNTVKLQYSAGV CCCCCEEEEEEECCC	28.03	23749301
142	Ubiquitination	QAAEEVTKEPRGLLM HHHHHHHCCCCHHHH	71.54	23749301
176	Ubiquitination	VDIAKSDKDFVIGFI EEEHHCCCCEEEEEE	61.22	18433149
209	Ubiquitination	PGVGLDDKGDALGQQ CCCCCCCCCCCCCCC	59.36	23749301
219	Phosphorylation	ALGQQYRTVDDVVST CCCCCEEEHHHHHHC	24.29	28889911
228	Phosphorylation	DDVVSTGSDIIIVGR HHHHHCCCCEEEECC	26.23	28889911
240	Ubiquitination	VGRGLFAKGRDAKVE ECCCHHHCCCCCEEC	47.33	23749301
245	Ubiquitination	FAKGRDAKVEGERYR HHCCCCCEECCHHHH	45.14	22817900
253	Ubiquitination	VEGERYRKAGWEAYL ECCHHHHHHCHHHHH	42.17	23749301

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
-	K	Ubiquitination	E3 ubiquitin ligase	SSM4	P40318	PMID:22199232

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of PYRF_YEAST !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of PYRF_YEAST !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
RNQ1_YEAST	RNQ1	physical	11805837
PYRF_YEAST	URA3	physical	10688190
ARO9_YEAST	ARO9	genetic	10207060
ARO80_YEAST	ARO80	genetic	10207060
GAP1_YEAST	GAP1	genetic	10207060
FUR4_YEAST	FUR4	genetic	9829833
YJF5_YEAST	YJL055W	genetic	18186026
PYRF_YEAST	URA3	physical	19435314
PMP1_YEAST	PMP1	physical	26404137

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"A multidimensional chromatography technology for in-depthphosphoproteome analysis.";
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
Mol. Cell. Proteomics 7:1389-1396(2008).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-219 AND SER-228, ANDMASS SPECTROMETRY.
PubMed: 18407956

Ubiquitylation

"A proteomics approach to understanding protein ubiquitination.";
Peng J., Schwartz D., Elias J.E., Thoreen C.C., Cheng D.,Marsischky G., Roelofs J., Finley D., Gygi S.P.;
Nat. Biotechnol. 21:921-926(2003).
Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-93; LYS-209 AND LYS-253,AND MASS SPECTROMETRY.
PubMed: 12872131