PEPD_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: PEPD_HUMAN
• UniProt AC: P12955
• Protein Name: Xaa-Pro dipeptidase
• Gene Name: PEPD
• Organism: Homo sapiens (Human).
• Sequence Length: 493
• Protein Description: Splits dipeptides with a prolyl or hydroxyprolyl residue in the C-terminal position. Plays an important role in collagen metabolism because the high level of iminoacids in collagen..
• Protein Sequence: MAAATGPSFWLGNETLKVPLALFALNRQRLCERLRKNPAVQAGSIVVLQGGEETQRYCTDTGVLFRQESFFHWAFGVTEPGCYGVIDVDTGKSTLFVPRLPASHATWMGKIHSKEHFKEKYAVDDVQYVDEIASVLTSQKPSVLLTLRGVNTDSGSVCREASFDGISKFEVNNTILHPEIVECRVFKTDMELEVLRYTNKISSEAHREVMKAVKVGMKEYELESLFEHYCYSRGGMRHSSYTCICGSGENSAVLHYGHAGAPNDRTIQNGDMCLFDMGGEYYCFASDITCSFPANGKFTADQKAVYEAVLRSSRAVMGAMKPGVWWPDMHRLADRIHLEELAHMGILSGSVDAMVQAHLGAVFMPHGLGHFLGIDVHDVGGYPEGVERIDEPGLRSLRTARHLQPGMVLTVEPGIYFIDHLLDEALADPARASFLNREVLQRFRGFGGVRIEEDVVVTDSGIELLTCVPRTVEEIEACMAGCDKAFTPFSGPK

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Acetylation	------MAAATGPSF ------CCCCCCCCC	13.05	22223895
5	Phosphorylation	---MAAATGPSFWLG ---CCCCCCCCCCCC	44.92	20068231
8	Phosphorylation	MAAATGPSFWLGNET CCCCCCCCCCCCCCC	30.33	20068231
15	Phosphorylation	SFWLGNETLKVPLAL CCCCCCCCCHHCHHH	35.56	20068231
17	Acetylation	WLGNETLKVPLALFA CCCCCCCHHCHHHHH	49.58	19816731
36	Ubiquitination	RLCERLRKNPAVQAG HHHHHHHHCCCCCCC	72.39	22053931
108	Sulfoxidation	PASHATWMGKIHSKE CCCCCCHHCCCCCHH	3.21	30846556
110	Acetylation	SHATWMGKIHSKEHF CCCCHHCCCCCHHHH	22.57	23954790
110	Ubiquitination	SHATWMGKIHSKEHF CCCCHHCCCCCHHHH	22.57	-
113	Phosphorylation	TWMGKIHSKEHFKEK CHHCCCCCHHHHHHH	43.12	28348404
114	Ubiquitination	WMGKIHSKEHFKEKY HHCCCCCHHHHHHHH	40.83	-
120	Ubiquitination	SKEHFKEKYAVDDVQ CHHHHHHHHCCCCHH	38.82	-
128	Phosphorylation	YAVDDVQYVDEIASV HCCCCHHHHHHHHHH	15.09	-
142	Phosphorylation	VLTSQKPSVLLTLRG HHHCCCCCEEEEECC	32.22	-
156	Phosphorylation	GVNTDSGSVCREASF CCCCCCCCCCCCCCC	23.23	28857561
167	Phosphorylation	EASFDGISKFEVNNT CCCCCCCCEEEECCE	36.50	8900231
188	Phosphorylation	VECRVFKTDMELEVL EEEEEEECCHHHHHH	29.16	16097034
190	Sulfoxidation	CRVFKTDMELEVLRY EEEEECCHHHHHHHH	8.40	21406390
196	Methylation	DMELEVLRYTNKISS CHHHHHHHHCCCCCH	41.21	115486993
200	Ubiquitination	EVLRYTNKISSEAHR HHHHHCCCCCHHHHH	35.84	-
303	Ubiquitination	GKFTADQKAVYEAVL CCCCCCHHHHHHHHH	40.13	21906983
306	Phosphorylation	TADQKAVYEAVLRSS CCCHHHHHHHHHHHC	11.75	20068231
484	Ubiquitination	ACMAGCDKAFTPFSG HHHCCCCCCCCCCCC	49.85	-
487	Phosphorylation	AGCDKAFTPFSGPK- CCCCCCCCCCCCCC-	28.43	27499020
490	Phosphorylation	DKAFTPFSGPK---- CCCCCCCCCCC----	56.22	23403867
493	Acetylation	FTPFSGPK------- CCCCCCCC-------	76.21	90257

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of PEPD_HUMAN !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of PEPD_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of PEPD_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
OSTP_HUMAN	SPP1	physical	21988832
ASSY_HUMAN	ASS1	physical	22863883
BCCIP_HUMAN	BCCIP	physical	22863883
CAN1_HUMAN	CAPN1	physical	22863883
CASP7_HUMAN	CASP7	physical	22863883
DLDH_HUMAN	DLD	physical	22863883
DPP3_HUMAN	DPP3	physical	22863883
IF5A1_HUMAN	EIF5A	physical	22863883
FTO_HUMAN	FTO	physical	22863883
GBP2_HUMAN	GBP2	physical	22863883
GUAD_HUMAN	GDA	physical	22863883
PSF2_HUMAN	GINS2	physical	22863883
GLSK_HUMAN	GLS	physical	22863883
GNS_HUMAN	GNS	physical	22863883
LDH6B_HUMAN	LDHAL6B	physical	22863883
MVD1_HUMAN	MVD	physical	22863883
NDRG1_HUMAN	NDRG1	physical	22863883
KS6A1_HUMAN	RPS6KA1	physical	22863883
AATC_HUMAN	GOT1	physical	26344197
CH10_HUMAN	HSPE1	physical	26344197
PSMG4_HUMAN	PSMG4	physical	26344197
TNG2_HUMAN	TANGO2	physical	26344197

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• 170100: Prolidase deficiency (PD)
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Acetylation

"Lys-N and trypsin cover complementary parts of the phosphoproteome ina refined SCX-based approach.";
Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J.,Mohammed S.;
Anal. Chem. 81:4493-4501(2009).
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, AND MASS SPECTROMETRY.
PubMed: 19413330

Phosphorylation

"Global phosphoproteome analysis on human HepG2 hepatocytes usingreversed-phase diagonal LC.";
Gevaert K., Staes A., Van Damme J., De Groot S., Hugelier K.,Demol H., Martens L., Goethals M., Vandekerckhove J.;
Proteomics 5:3589-3599(2005).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-188, AND MASSSPECTROMETRY.
PubMed: 16097034

"Large-scale characterization of HeLa cell nuclear phosphoproteins.";
Beausoleil S.A., Jedrychowski M., Schwartz D., Elias J.E., Villen J.,Li J., Cohn M.A., Cantley L.C., Gygi S.P.;
Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-487, AND MASSSPECTROMETRY.
PubMed: 15302935