OTUD5_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: OTUD5_MOUSE
• UniProt AC: Q3U2S4
• Protein Name: OTU domain-containing protein 5
• Gene Name: Otud5
• Organism: Mus musculus (Mouse).
• Sequence Length: 566
• Protein Description: Deubiquitinating enzyme that functions as negative regulator of the innate immune system. Acts via TRAF3 deubiquitination and subsequent suppression of type I interferon (IFN) production. Has peptidase activity towards 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. Can also cleave 'Lys-11'-linked ubiquitin chains (in vitro) (By similarity)..
• Protein Sequence: MTILPKKKPPPPDADPANEPPPPGPLPPAPRRGAGVGVGGGGTGVGGGERDRDSGVVGARPRASPPPQGPLPGPPGALHRWALAVPPGAVAGPRPQQASPPPCGGPGGPGGGPGDALGATTAGVGAAGVVVGVGGTVGVGGCCSGPGHSKRRRQAPGVGAVGGASPEREEVGAGYNSEDEYEAAAARIEAMDPATVEQQEHWFEKALRDKKGFIIKQMKEDGACLFRAVADQVYGDQDMHEVVRKHCMDYLMKNADYFSNYVTEDFTTYINRKRKNNCHGNHIEMQAMAEMYNRPVEVYQYSTEPINTFHGIHQNEDEPIRVSYHRNIHYNSVVNPNKATIGVGLGLPSFKPGFAEQSLMKNAIKTSEESWIEQQMLEDKKRATDWEATNEAIEEQVARESYLQWLRDQEKQARQVRGPSQPRKASATCSSATAAASSGLEEWTSRSPRQRSSASSPEHPELHAELGIKPPSPGTVLALAKPPSPCAPGTSSQFSAGGDRATSPLVSLYPALECRALIQQMSPSAFGLNDWDDDEILASVLAVSQQEYLDSMKKNKVHREPPPDKS

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
54	Phosphorylation	GGERDRDSGVVGARP CCCCCCCCCCCCCCC	34.09	25266776
64	Phosphorylation	VGARPRASPPPQGPL CCCCCCCCCCCCCCC	38.94	25521595
165	Phosphorylation	VGAVGGASPEREEVG CCCCCCCCCCHHHCC	30.48	25521595
175	Phosphorylation	REEVGAGYNSEDEYE HHHCCCCCCCHHHHH	18.25	25521595
177	Phosphorylation	EVGAGYNSEDEYEAA HCCCCCCCHHHHHHH	37.63	25521595
181	Phosphorylation	GYNSEDEYEAAAARI CCCCHHHHHHHHHHH	24.16	26643407
351	Ubiquitination	GLGLPSFKPGFAEQS ECCCCCCCCCHHHHH	48.87	-
366	Phosphorylation	LMKNAIKTSEESWIE HHHHHHHHCHHHHHH	34.95	22802335
380	Ubiquitination	EQQMLEDKKRATDWE HHHHHHHHHHCCCHH	34.48	-
381	Ubiquitination	QQMLEDKKRATDWEA HHHHHHHHHCCCHHH	60.63	-
426	Phosphorylation	PSQPRKASATCSSAT CCCCCCCCCCCHHHH	27.44	25266776
428	Phosphorylation	QPRKASATCSSATAA CCCCCCCCCHHHHHH	15.49	25777480
430	Phosphorylation	RKASATCSSATAAAS CCCCCCCHHHHHHHH	20.75	25777480
431	Phosphorylation	KASATCSSATAAASS CCCCCCHHHHHHHHH	31.61	25777480
433	Phosphorylation	SATCSSATAAASSGL CCCCHHHHHHHHHCH	20.83	26643407
437	Phosphorylation	SSATAAASSGLEEWT HHHHHHHHHCHHHHH	21.88	26643407
438	Phosphorylation	SATAAASSGLEEWTS HHHHHHHHCHHHHHH	42.54	25266776
444	Phosphorylation	SSGLEEWTSRSPRQR HHCHHHHHHCCHHHH	19.97	25266776
445	Phosphorylation	SGLEEWTSRSPRQRS HCHHHHHHCCHHHHC	31.66	23984901
447	Phosphorylation	LEEWTSRSPRQRSSA HHHHHHCCHHHHCCC	24.97	21082442
492	Phosphorylation	PCAPGTSSQFSAGGD CCCCCCCCCCCCCCC	34.77	24719451
495	Phosphorylation	PGTSSQFSAGGDRAT CCCCCCCCCCCCCCC	20.54	24719451
502	Phosphorylation	SAGGDRATSPLVSLY CCCCCCCCCCHHHHH	31.60	23527152
503	Phosphorylation	AGGDRATSPLVSLYP CCCCCCCCCHHHHHH	18.43	25521595
507	Phosphorylation	RATSPLVSLYPALEC CCCCCHHHHHHHHHH	29.84	26643407
509	Phosphorylation	TSPLVSLYPALECRA CCCHHHHHHHHHHHH	4.48	25777480

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of OTUD5_MOUSE !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
177	S	Phosphorylation	Phosphorylation at Ser-177 is required for deubiquitinating activity.	21183079
177	S	ubiquitylation	Phosphorylation at Ser-177 is required for deubiquitinating activity.	21183079

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of OTUD5_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
UBR5_MOUSE	Ubr5	physical	25470037
FMNL1_HUMAN	FMNL1	physical	26496610
GOGA4_HUMAN	GOLGA4	physical	26496610
NHS_HUMAN	NHS	physical	26496610
POGZ_HUMAN	POGZ	physical	26496610
UACA_HUMAN	UACA	physical	26496610
EP15R_HUMAN	EPS15L1	physical	26496610
MROH1_HUMAN	MROH1	physical	26496610

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"Large-scale phosphorylation analysis of mouse liver.";
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.;
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-503, AND MASSSPECTROMETRY.
PubMed: 17242355