dbPTM

NDE1_MOUSE - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	NDE1_MOUSE
UniProt AC	Q9CZA6
Protein Name	Nuclear distribution protein nudE homolog 1
Gene Name	Nde1
Organism	Mus musculus (Mouse).
Sequence Length	344
Subcellular Localization	Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle. Chromosome, centromere, kinetochore. Cleavage furrow. Localizes to the interphase and S phase centrosome. During mitosis, p
Protein Description	Required for centrosome duplication and formation and function of the mitotic spindle. Essential for the development of the cerebral cortex. May regulate the production of neurons by controlling the orientation of the mitotic spindle during division of cortical neuronal progenitors of the proliferative ventricular zone of the brain. Orientation of the division plane perpendicular to the layers of the cortex gives rise to two proliferative neuronal progenitors whereas parallel orientation of the division plane yields one proliferative neuronal progenitor and a post-mitotic neuron. A premature shift towards a neuronal fate within the progenitor population may result in an overall reduction in the final number of neurons and an increase in the number of neurons in the deeper layers of the cortex..
Protein Sequence	MEDSGKTFESEEEETNYWRDLAMTYKQRAENTQEELREFQEGSREYEAELEAQLQQIETRNRDLLSENNRLRMELESVKEKFEMQHSEGYRQISALEDDLAQTKAIKDQLQKYIRELEQANDDLERAKRATIMSLEDFEQRLNQAIERNAFLESELDEKENLLESVQRLKDEARDLRQELAVQQKQDKPRTPMPGSGQAKRTDMAVQATGSVPSTPVAHRGPSSGLNTPGMFRRGLDSSTSGTPLTPAARISALNIVGDLLRKVGALESKLASCRNFMYDQSPSRTSGPASGRGTKNRDGVDRRPGSTSVGDKGSGKRLEFGKPASEPASPALPSAQGVVKLLL

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
7	Phosphorylation	-MEDSGKTFESEEEE -CCCCCCCCCCHHHH	35.84	26643407
10	Phosphorylation	DSGKTFESEEEETNY CCCCCCCCHHHHHHH	46.23	26643407
15	Phosphorylation	FESEEEETNYWRDLA CCCHHHHHHHHHHHH	36.57	30635358
17	Phosphorylation	SEEEETNYWRDLAMT CHHHHHHHHHHHHHH	14.83	30635358
90	Phosphorylation	EMQHSEGYRQISALE HHHHCHHHHHHHHHH	8.69	-
211	Phosphorylation	MAVQATGSVPSTPVA EEEEECCCCCCCCCC	26.88	19060867
214	Phosphorylation	QATGSVPSTPVAHRG EECCCCCCCCCCCCC	43.09	21183079
215	Phosphorylation	ATGSVPSTPVAHRGP ECCCCCCCCCCCCCC	18.73	19060867
223	Phosphorylation	PVAHRGPSSGLNTPG CCCCCCCCCCCCCCC	39.60	28066266
224	Phosphorylation	VAHRGPSSGLNTPGM CCCCCCCCCCCCCCH	50.89	28066266
228	Phosphorylation	GPSSGLNTPGMFRRG CCCCCCCCCCHHHCC	26.68	26824392
238	Phosphorylation	MFRRGLDSSTSGTPL HHHCCCCCCCCCCCC	40.33	24759943
239	Phosphorylation	FRRGLDSSTSGTPLT HHCCCCCCCCCCCCC	26.68	23984901
240	Phosphorylation	RRGLDSSTSGTPLTP HCCCCCCCCCCCCCH	34.82	23984901
241	Phosphorylation	RGLDSSTSGTPLTPA CCCCCCCCCCCCCHH	42.49	23984901
243	Phosphorylation	LDSSTSGTPLTPAAR CCCCCCCCCCCHHHH	18.20	22817900
246	Phosphorylation	STSGTPLTPAARISA CCCCCCCCHHHHHHH	16.46	21082442
274	S-palmitoylation	LESKLASCRNFMYDQ HHHHHHHCCCCCCCC	3.24	-
278	Oxidation	LASCRNFMYDQSPSR HHHCCCCCCCCCCCC	4.04	17242355
279	Phosphorylation	ASCRNFMYDQSPSRT HHCCCCCCCCCCCCC	13.49	29895711
282	Phosphorylation	RNFMYDQSPSRTSGP CCCCCCCCCCCCCCC	23.19	26824392
284	Phosphorylation	FMYDQSPSRTSGPAS CCCCCCCCCCCCCCC	54.13	29895711
291	Phosphorylation	SRTSGPASGRGTKNR CCCCCCCCCCCCCCC	31.96	29514104
293	Methylation	TSGPASGRGTKNRDG CCCCCCCCCCCCCCC	47.30	58858713
295	Phosphorylation	GPASGRGTKNRDGVD CCCCCCCCCCCCCCC	24.64	23832136
307	Phosphorylation	GVDRRPGSTSVGDKG CCCCCCCCCCCCCCC	21.86	20139300
307 (in isoform 3)	Phosphorylation	-	21.86	19854140
308	Phosphorylation	VDRRPGSTSVGDKGS CCCCCCCCCCCCCCC	33.09	20139300
308 (in isoform 3)	Phosphorylation	-	33.09	19854140
309	Phosphorylation	DRRPGSTSVGDKGSG CCCCCCCCCCCCCCC	26.29	20139300
315	Phosphorylation	TSVGDKGSGKRLEFG CCCCCCCCCCCCCCC	47.05	20139300
315 (in isoform 3)	Phosphorylation	-	47.05	19854140
326	Phosphorylation	LEFGKPASEPASPAL CCCCCCCCCCCCCCC	53.27	26824392
330	Phosphorylation	KPASEPASPALPSAQ CCCCCCCCCCCCCCC	22.78	26824392
335	Phosphorylation	PASPALPSAQGVVKL CCCCCCCCCCCHHHH	33.79	28066266

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
90	Y	Phosphorylation	Kinase	FGFR1	P11362	PSP
279	Y	Phosphorylation	Kinase	FGFR1	P11362	PSP

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
246	T	Phosphorylation		21529751
Phosphorylation at Thr-246 is essential for the G2/M transition.

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of NDE1_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
LIS1_HUMAN	PAFAH1B1	physical	11163258
LIS1_MOUSE	Pafah1b1	physical	11163258
LIS1_HUMAN	PAFAH1B1	physical	20360068
DIXC1_HUMAN	DIXDC1	physical	20360068
NDEL1_HUMAN	NDEL1	physical	20360068
SYNE1_HUMAN	SYNE1	physical	20360068
NDE1_HUMAN	NDE1	physical	20360068
CCSE2_HUMAN	CCSER2	physical	20360068
RFA2_HUMAN	RPA2	physical	20360068
DYL1_MOUSE	Dynll1	physical	21911489
DC1I1_MOUSE	Dync1i1	physical	21911489
SMC3_MOUSE	Smc3	physical	25245017
SMC1A_MOUSE	Smc1a	physical	25245017
RAD21_MOUSE	Rad21	physical	25245017
SMCA5_MOUSE	Smarca5	physical	25245017
P53_MOUSE	Trp53	genetic	25245017

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of NDE1_MOUSE

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Human mutations in NDE1 cause extreme microcephaly withlissencephaly."; Alkuraya F.S., Cai X., Emery C., Mochida G.H., Al-Dosari M.S.,Felie J.M., Hill R.S., Barry B.J., Partlow J.N., Gascon G.G.,Kentab A., Jan M., Shaheen R., Feng Y., Walsh C.A.; Am. J. Hum. Genet. 88:536-547(2011). Cited for: PHOSPHORYLATION AT THR-246.	21529751 [Abstract]
"Large-scale phosphorylation analysis of mouse liver."; Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.; Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-246, AND MASSSPECTROMETRY.	17242355 [Abstract]

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