MYOD1_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: MYOD1_MOUSE
• UniProt AC: P10085
• Protein Name: Myoblast determination protein 1
• Gene Name: Myod1
• Organism: Mus musculus (Mouse).
• Sequence Length: 318
• Subcellular Localization: Nucleus .
• Protein Description: Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. [PubMed: 16901893 Together with MYF5 and MYOG, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins]
• Protein Sequence: MELLSPPLRDIDLTGPDGSLCSFETADDFYDDPCFDSPDLRFFEDLDPRLVHMGALLKPEEHAHFPTAVHPGPGAREDEHVRAPSGHHQAGRCLLWACKACKRKTTNADRRKAATMRERRRLSKVNEAFETLKRCTSSNPNQRLPKVEILRNAIRYIEGLQALLRDQDAAPPGAAAFYAPGPLPPGRGSEHYSGDSDASSPRSNCSDGMMDYSGPPSGPRRQNGYDTAYYSEAARESRPGKSAAVSSLDCLSSIVERISTDSPAAPALLLADAPPESPPGPPEGASLSDTEQGTQTPSPDAAPQCPAGSNPNAIYQVL

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
5	Phosphorylation	---MELLSPPLRDID ---CCCCCCCCCCCC	36.78	14749395
30	Phosphorylation	FETADDFYDDPCFDS EECCCHHCCCCCCCC	26.82	12415271
37	Phosphorylation	YDDPCFDSPDLRFFE CCCCCCCCCCCCHHH	10.48	-
99	Acetylation	RCLLWACKACKRKTT HHHHHHHHHHCCCCC	49.42	10619020
102	Acetylation	LWACKACKRKTTNAD HHHHHHHCCCCCCHH	62.89	10619020
104	Acetylation	ACKACKRKTTNADRR HHHHHCCCCCCHHHH	45.24	10619020
104	Methylation	ACKACKRKTTNADRR HHHHHCCCCCCHHHH	45.24	-
115	Phosphorylation	ADRRKAATMRERRRL HHHHHHHHHHHHHHH	22.94	17761773
133	Ubiquitination	NEAFETLKRCTSSNP HHHHHHHHHHHCCCC	53.50	-
156	Phosphorylation	ILRNAIRYIEGLQAL HHHHHHHHHHHHHHH	9.37	21454680
200	Phosphorylation	SGDSDASSPRSNCSD CCCCCCCCCCCCCCC	26.50	10942602
203	Phosphorylation	SDASSPRSNCSDGMM CCCCCCCCCCCCCCC	45.64	21659605
212	Phosphorylation	CSDGMMDYSGPPSGP CCCCCCCCCCCCCCC	9.53	12415271
237	Phosphorylation	YSEAARESRPGKSAA HCHHHHHHCCCCCHH	37.92	10838091
262	Phosphorylation	VERISTDSPAAPALL HHHHCCCCCCCCHHH	19.27	-
277	Phosphorylation	LADAPPESPPGPPEG HCCCCCCCCCCCCCC	42.01	-
296	Phosphorylation	DTEQGTQTPSPDAAP CCCCCCCCCCCCCCC	26.10	-
298	Phosphorylation	EQGTQTPSPDAAPQC CCCCCCCCCCCCCCC	38.51	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
5	S	Phosphorylation	Kinase	CDK1	P06493	PSP
5	S	Phosphorylation	Kinase	CDK1	P11440	PSP
30	Y	Phosphorylation	Kinase	ABL1	P00519	GPS
30	Y	Phosphorylation	Kinase	ABL1	P00520	PhosphoELM
30	Y	Phosphorylation	Kinase	ABL-FAMILY	-	GPS
156	Y	Phosphorylation	Kinase	MEK1	Q02750	PSP
156	Y	Phosphorylation	Kinase	MAP2K1	P31938	GPS
200	S	Phosphorylation	Kinase	CDK1	P06493	PSP
200	S	Phosphorylation	Kinase	CDK1	P11440	PSP
200	S	Phosphorylation	Kinase	CDK2	P24941	PSP
200	S	Phosphorylation	Kinase	CDK2	P97377	PSP
212	Y	Phosphorylation	Kinase	ABL1	P00519	GPS
212	Y	Phosphorylation	Kinase	ABL1	P00520	PhosphoELM
212	Y	Phosphorylation	Kinase	ABL-FAMILY	-	GPS
237	S	Phosphorylation	Kinase	MOS	P00536	PSP
-	K	Ubiquitination	E3 ubiquitin ligase	Fbxo32	Q9CPU7	PMID:22199232

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
104	K	Methylation	Methylation at Lys-104 by EHMT2/G9a inhibits myogenic activity.	-

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of MYOD1_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
KAT2B_MOUSE	Kat2b	physical	11285237
HDAC1_MOUSE	Hdac1	physical	11285237
NUPR1_MOUSE	Nupr1	physical	19723804
ANM5_MOUSE	Prmt5	physical	17043109
MYOD1_MOUSE	Myod1	physical	20211142
TFE2_MOUSE	Tcf3	physical	20211142
KAT2B_MOUSE	Kat2b	physical	9659901
EP300_MOUSE	Ep300	physical	14749395
KAT2B_MOUSE	Kat2b	physical	14749395
KDM1A_HUMAN	KDM1A	physical	20833138
NCOA2_MOUSE	Ncoa2	physical	15563453
NCOA1_MOUSE	Ncoa1	physical	15563453
BHE41_MOUSE	Bhlhe41	physical	12657651
MBB1A_MOUSE	Mybbp1a	physical	22333916
ASCL3_MOUSE	Ascl3	physical	11784080
TFE2_MOUSE	Tcf3	physical	11711431
MP2K1_MOUSE	Map2k1	physical	21454680
FBX32_MOUSE	Fbxo32	physical	21454680

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.