MDM4_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: MDM4_MOUSE
• UniProt AC: O35618
• Protein Name: Protein Mdm4
• Gene Name: Mdm4
• Organism: Mus musculus (Mouse).
• Sequence Length: 489
• Subcellular Localization: Nucleus.
• Protein Description: Inhibits p53/TP53- and TP73/p73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Inhibits degradation of MDM2. Can reverse MDM2-targeted degradation of TP53 while maintaining suppression of TP53 transactivation and apoptotic functions. The short isoform is a more potent inhibitor of TP53 activity than the long isoform..
• Protein Sequence: MTSHSTSAQCSASDSACRISSEQISQVRPKLQLLKILHAAGAQGEVFTMKEVMHYLGQYIMVKQLYDQQEQHMVYCGGDLLGDLLGCQSFSVKDPSPLYDMLRKNLVTSASINTDAAQTLALAQDHTMDFPSQDRLKHGATEYSNPRKRTEEEDTHTLPTSRHKCRDSRADEDLIEHLSQDETSRLDLDFEEWDVAGLPWWFLGNLRNNCIPKSNGSTDLQTNQDIGTAIVSDTTDDLWFLNETVSEQLGVGIKVEAANSEQTSEVGKTSNKKTVEVGKDDDLEDSRSLSDDTDVELTSEDEWQCTECKKFNSPSKRYCFRCWALRKDWYSDCSKLTHSLSTSNITAIPEKKDNEGIDVPDCRRTISAPVVRPKDGYLKEEKPRFDPCNSVGFLDLAHSSESQEIISSAREQTDIFSEQKAETESMEDFQNVLKPCSLCEKRPRDGNIIHGKTSHLTTCFHCARRLKKSGASCPACKKEIQLVIKVFIA

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
179	Phosphorylation	EDLIEHLSQDETSRL HHHHHHHCCCCCCCC	37.18	-
288	Phosphorylation	DDLEDSRSLSDDTDV CCCCHHHCCCCCCCC	35.86	-
337	Phosphorylation	YSDCSKLTHSLSTSN HHHHHHHHHCCCCCC	17.10	23984901
339	Phosphorylation	DCSKLTHSLSTSNIT HHHHHHHCCCCCCEE	20.84	29472430
341	Phosphorylation	SKLTHSLSTSNITAI HHHHHCCCCCCEEEC	32.57	22942356
342	Phosphorylation	KLTHSLSTSNITAIP HHHHCCCCCCEEECC	30.96	22345495
343	Phosphorylation	LTHSLSTSNITAIPE HHHCCCCCCEEECCC	23.90	29895711
346	Phosphorylation	SLSTSNITAIPEKKD CCCCCCEEECCCCCC	23.84	25195567
365	Phosphorylation	DVPDCRRTISAPVVR CCCCCCCEECCCEEC	10.42	26745281
367	Phosphorylation	PDCRRTISAPVVRPK CCCCCEECCCEECCC	26.38	23984901
402	Phosphorylation	DLAHSSESQEIISSA HCCCCHHHHHHHHHH	34.75	22556425
485	Methylation	KEIQLVIKVFIA--- HHHHHHEEEHHC---	24.50	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
341	S	Phosphorylation	Kinase	CHEK2	Q9Z265	Uniprot
367	S	Phosphorylation	Kinase	CHEK1	O14757	GPS
367	S	Phosphorylation	Kinase	CHEK1	O35280	Uniprot
367	S	Phosphorylation	Kinase	CHEK2	Q9Z265	Uniprot
-	K	Ubiquitination	E3 ubiquitin ligase	Mdm2	P23804	PMID:22199232

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
341	S	Phosphorylation	Phosphorylation at Ser-341 also induces ubiquitination and degradation but to a lower extent (By similarity).	-
341	S	ubiquitylation	Phosphorylation at Ser-341 also induces ubiquitination and degradation but to a lower extent (By similarity).	-
367	S	Phosphorylation	Phosphorylation at Ser-367 promotes interaction with YWHAG and subsequent ubiquitination and degradation.	-
367	S	ubiquitylation	Phosphorylation at Ser-367 promotes interaction with YWHAG and subsequent ubiquitination and degradation.	-

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of MDM4_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
MDM2_MOUSE	Mdm2	physical	20697359
CDN1A_MOUSE	Cdkn1a	physical	21148311
1433G_MOUSE	Ywhag	physical	21148311
P53_HUMAN	TP53	physical	21572037
MDM2_MOUSE	Mdm2	physical	21730132
P53_MOUSE	Trp53	physical	21730132
UB2D3_HUMAN	UBE2D3	physical	21572037
1433G_MOUSE	Ywhag	physical	24190973
MDM2_MOUSE	Mdm2	physical	19573810
P53_MOUSE	Trp53	physical	19573810
MDM2_MOUSE	Mdm2	physical	25981963
P53_MOUSE	Trp53	physical	25981963

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.