dbPTM

CDN1A_MOUSE - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	CDN1A_MOUSE
UniProt AC	P39689
Protein Name	Cyclin-dependent kinase inhibitor 1
Gene Name	Cdkn1a
Organism	Mus musculus (Mouse).
Sequence Length	159
Subcellular Localization	Cytoplasm. Nucleus .
Protein Description	May be involved in p53/TP53 mediated inhibition of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex. [PubMed: 25329316 Inhibits DNA synthesis by DNA polymerase delta by competing with POLD3 for PCNA binding (By similarity]
Protein Sequence	MSNPGDVRPVPHRSKVCRCLFGPVDSEQLRRDCDALMAGCLQEARERWNFDFVTETPLEGNFVWERVRSLGLPKVYLSPGSRSRDDLGGDKRPSTSSALLQGPAPEDHVALSLSCTLVSERPEDSPGGPGTSQGRKRRQTSLTDFYHSKRRLVFCKRKP

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Acetylation	------MSNPGDVRP ------CCCCCCCCC	52.29	-
2	Phosphorylation	------MSNPGDVRP ------CCCCCCCCC	52.29	25266776
76	Phosphorylation	SLGLPKVYLSPGSRS HCCCCEEEECCCCCC	13.95	21743459
78	Phosphorylation	GLPKVYLSPGSRSRD CCCEEEECCCCCCCC	14.99	26824392
81	Phosphorylation	KVYLSPGSRSRDDLG EEEECCCCCCCCCCC	30.39	22345495
112	Phosphorylation	PEDHVALSLSCTLVS CCCEEEEEEEEEEEE	14.43	-
114	Phosphorylation	DHVALSLSCTLVSER CEEEEEEEEEEEECC	11.15	23649490
119	Phosphorylation	SLSCTLVSERPEDSP EEEEEEEECCCCCCC	30.88	23649490
125	Phosphorylation	VSERPEDSPGGPGTS EECCCCCCCCCCCCH	24.63	23649490
140	Phosphorylation	QGRKRRQTSLTDFYH HHHHHCCCCHHHHHH	24.72	21791608
141	Phosphorylation	GRKRRQTSLTDFYHS HHHHCCCCHHHHHHH	22.42	21791608

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
76	Y	Phosphorylation	Kinase	ABL1	P00519	GPS
78	S	Phosphorylation	Kinase	NUAK1	O60285	PSP
78	S	Phosphorylation	Kinase	NUAK1	Q641K5	Uniprot
112	S	Phosphorylation	Kinase	GSK3-BETA	Q9WV60	Uniprot
140	T	Phosphorylation	Kinase	AKT1	P31749	PSP
140	T	Phosphorylation	Kinase	AKT1	P31750	Uniprot
140	T	Phosphorylation	Kinase	CHEK1	O14757	GPS
140	T	Phosphorylation	Kinase	PIM1	P06803	Uniprot
140	T	Phosphorylation	Kinase	PIM2	Q62070	Uniprot
140	T	Phosphorylation	Kinase	PKA	-	Uniprot
141	S	Phosphorylation	Kinase	AKT1	P31749	PSP
141	S	Phosphorylation	Kinase	CHEK1	O14757	GPS
141	S	Phosphorylation	Kinase	NUAK1	O60285	PSP
141	S	Phosphorylation	Kinase	NUAK1	Q641K5	Uniprot
-	K	Ubiquitination	E3 ubiquitin ligase	Mkrn1	Q9QXP6	PMID:22199232
-	K	Ubiquitination	E3 ubiquitin ligase	Skp2	Q9Z0Z3	PMID:22199232

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Reference
2	S	ubiquitylation	-
Ubiquitination at Ser-2 leads to degradation by the proteasome pathway.
78	S	Phosphorylation	25329316
UV radiation-induced phosphorylation at Ser-78 and Ser-141 by NUAK1 leads to its degradation.
112	S	Phosphorylation	25329316
Phosphorylation at Ser-112 by GSK3-beta enhances ubiquitination by the DCX(DTL) complex (By similarity).
112	S	ubiquitylation	25329316
Phosphorylation at Ser-112 by GSK3-beta enhances ubiquitination by the DCX(DTL) complex (By similarity).
136	K	Acetylation	20154723
Acetylated in vitro on Lys-136, Lys-149, Lys-156 and Lys-158.
140	T	Phosphorylation	25329316
Phosphorylation of Thr-140 by PIM1 results in the relocation of CDKN1A to the cytoplasm and enhanced CDKN1A protein stability.
140	T	Phosphorylation	25329316
Phosphorylation of Thr-140 by PIM2 enhances its stability and inhibits cell proliferation.
140	T	Phosphorylation	25329316
Phosphorylation of Thr-140 or Ser-141 impairs binding to PCNA.
141	S	Phosphorylation	25329316
Phosphorylation of Thr-140 or Ser-141 impairs binding to PCNA.
141	S	Phosphorylation	25329316
UV radiation-induced phosphorylation at Ser-78 and Ser-141 by NUAK1 leads to its degradation.
149	K	Acetylation	20154723
Acetylated in vitro on Lys-136, Lys-149, Lys-156 and Lys-158.
156	K	Acetylation	20154723
Acetylated in vitro on Lys-136, Lys-149, Lys-156 and Lys-158.
158	K	Acetylation	20154723
Acetylated in vitro on Lys-136, Lys-149, Lys-156 and Lys-158.

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of CDN1A_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
CDK4_MOUSE	Cdk4	physical	18729080
CDK2_MOUSE	Cdk2	physical	18729080
CCND1_MOUSE	Ccnd1	physical	18729080
CCNB1_MOUSE	Ccnb1	physical	14749395
CDK1_MOUSE	Cdk1	physical	14749395
NF2L2_MOUSE	Nfe2l2	physical	19560419
PCNA_MOUSE	Pcna	physical	12970760
CCND1_MOUSE	Ccnd1	physical	12588994
HSP7C_MOUSE	Hspa8	physical	12588994
PCNA_MOUSE	Pcna	physical	24699724
DDB1_HUMAN	DDB1	physical	24699724
PCNA_HUMAN	PCNA	physical	24699724
CDK2_MOUSE	Cdk2	physical	15107404

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of CDN1A_MOUSE

Related Literatures of Post-Translational Modification