KCO1_ARATH - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: KCO1_ARATH
• UniProt AC: Q8LBL1
• Protein Name: Two-pore potassium channel 1
• Gene Name: TPK1
• Organism: Arabidopsis thaliana (Mouse-ear cress).
• Sequence Length: 363
• Subcellular Localization: Vacuole membrane ; Multi-pass membrane protein . Tonoplast.
• Protein Description: Voltage-independent, large conductance and potassium-selective tonoplast ion channel. Regulated by cytoplasmic calcium and pH. Does not mediate slow-vacuolar (SV) ionic currents, but essential to establish VK currents. Has some permeability for Rb(+) and NH(4)(+), but none for Na(+), Cs(+) or Li(+). Involved in intracellular K(+) redistribution and/or K(+) retranslocation between different tissues..
• Protein Sequence: MSSDAARTPLLPTEKIDTMAQDFNLNSRTSSSRKRRLRRSRSAPRGDCMYNDDVKIDEPPPHPSKIPMFSDLNPNLRRVIMFLALYLTIGTLCFYLVRDQISGHKTSGVVDALYFCIVTMTTVGYGDLVPNSSASRLLACAFVFSGMVLVGHLLSRAADYLVEKQEALLVRAFHLRQSFGPTDILKELHTNKLRYKCYATCLVLVVLFIVGTIFLVMVEKMPVISAFYCVCSTVTTLGYGDKSFNSEAGRLFAVFWILTSSICLAQFFLYVAELNTENKQRALVKWVLTRRITNNDLEAADLDEDGVVGAAEFIVYKLKEMGKIDEKDISGIMDEFEQLDYDESGTLTTSDIVLAQTTSQIQR

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
27	Phosphorylation	AQDFNLNSRTSSSRK HHHCCCCCCCCHHHH		30407730
42	Phosphorylation	RRLRRSRSAPRGDCM HHHHHHCCCCCCCCC		19376835
178	Phosphorylation	RAFHLRQSFGPTDIL HHHHHHHHHCCHHHH		25561503

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of KCO1_ARATH !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
42	S	Phosphorylation	Phosphorylation at Ser-42 increases and stabilizes the interaction with 14-3-3 proteins.	17764516

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of KCO1_ARATH !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
14336_ARATH	GRF6	physical	17764516
14331_ARATH	GRF1	physical	17764516
HHP4_ARATH	HHP4	physical	24833385
HHP2_ARATH	HHP2	physical	24833385
UBC34_ARATH	UBC34	physical	24833385
POP3_ARATH	HS1	physical	24833385
CNIH1_ARATH	AT3G12180	physical	24833385
HSDD3_ARATH	AT2G43420	physical	24833385
CP21D_ARATH	AT3G66654	physical	24833385
SPCS1_ARATH	AT2G22425	physical	24833385
BET12_ARATH	ATBET12	physical	24833385
TRXH7_ARATH	TH7	physical	24833385

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.