GCR_RAT - dbPTM
GCR_RAT - PTM Information in dbPTM
Basic Information of Protein
UniProt ID GCR_RAT
UniProt AC P06536
Protein Name Glucocorticoid receptor
Gene Name Nr3c1
Organism Rattus norvegicus (Rat).
Sequence Length 795
Subcellular Localization Isoform A: Cytoplasm . Nucleus . Mitochondrion . Cytoplasm, cytoskeleton, spindle . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . After ligand activation, translocates from the cytoplasm to the nucleus.
Protein Description Receptor for glucocorticoids (GC). Has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE), both for nuclear and mitochondrial DNA, and as a modulator of other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Involved in chromatin remodeling. [PubMed: 12917342 Plays a role in rapid mRNA degradation by binding to the 5' UTR of target mRNAs and interacting with PNRC2 in a ligand-dependent manner which recruits the RNA helicase UPF1 and the mRNA-decapping enzyme DCP1A, leading to RNA decay (By similarity Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth (By similarity; Isoform A: Has transcriptional activation and repression activity. Mediates glucocorticoid-induced apoptosis. Promotes accurate chromosome segregation during mitosis. May act as a tumor suppressor. May play a negative role in adipogenesis through the regulation of lipolytic and antilipogenic gene expression.]
Protein Sequence MDSKESLAPPGRDEVPGSLLGQGRGSVMDFYKSLRGGATVKVSASSPSVAAASQADSKQQRILLDFSKGSTSNVQQRQQQQQQQQQQQQQQQQQQQPDLSKAVSLSMGLYMGETETKVMGNDLGYPQQGQLGLSSGETDFRLLEESIANLNRSTSVPENPKSSTSATGCATPTEKEFPKTHSDASSEQQNRKSQTGTNGGSVKLYPTDQSTFDLLKDLEFSAGSPSKDTNESPWRSDLLIDENLLSPLAGEDDPFLLEGNTNEDCKPLILPDTKPKIKDTGDTILSSPSSVALPQVKTEKDDFIELCTPGVIKQEKLGPVYCQASFSGTNIIGNKMSAISVHGVSTSGGQMYHYDMNTASLSQQQDQKPVFNVIPPIPVGSENWNRCQGSGEDSLTSLGALNFPGRSVFSNGYSSPGMRPDVSSPPSSSSAATGPPPKLCLVCSDEASGCHYGVLTCGSCKVFFKRAVEGQHNYLCAGRNDCIIDKIRRKNCPACRYRKCLQAGMNLEARKTKKKIKGIQQATAGVSQDTSENPNKTIVPAALPQLTPTLVSLLEVIEPEVLYAGYDSSVPDSAWRIMTTLNMLGGRQVIAAVKWAKAILGLRNLHLDDQMTLLQYSWMFLMAFALGWRSYRQSSGNLLCFAPDLIINEQRMSLPCMYDQCKHMLFVSSELQRLQVSYEEYLCMKTLLLLSSVPKEGLKSQELFDEIRMTYIKELGKAIVKREGNSSQNWQRFYQLTKLLDSMHEVVENLLTYCFQTFLDKTMSIEFPEMLAEIITNQIPKYSNGNIKKLLFHQK
Overview of Protein Modification Sites with Functional and Structural Information
Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations Modification Substrate Peptides
&
Secondary Structure		 ASA (%) Reference Orthologous
Protein Cluster
24MethylationGSLLGQGRGSVMDFY
HHHCCCCCCCHHHHH		26.73-
26PhosphorylationLLGQGRGSVMDFYKS
HCCCCCCCHHHHHHH		16.9525575281
43PhosphorylationGGATVKVSASSPSVA
CCCEEEEECCCHHHH		19.4027097102
45PhosphorylationATVKVSASSPSVAAA
CEEEEECCCHHHHHH		34.9627097102
46PhosphorylationTVKVSASSPSVAAAS
EEEEECCCHHHHHHH		22.4027097102
48PhosphorylationKVSASSPSVAAASQA
EEECCCHHHHHHHHC		26.7427097102
58AcetylationAASQADSKQQRILLD
HHHHCCCHHCEEEHH		51.5022902405
68AcetylationRILLDFSKGSTSNVQ
EEEHHHCCCCHHHHH		57.8022902405
100PhosphorylationQQQQPDLSKAVSLSM
HHHCCHHHHHHHHHH		26.4227097102
125PhosphorylationVMGNDLGYPQQGQLG
ECCCCCCCCCCCCCC		12.7427097102
134PhosphorylationQQGQLGLSSGETDFR
CCCCCCCCCCCHHHH		34.2027097102
135PhosphorylationQGQLGLSSGETDFRL
CCCCCCCCCCHHHHH		45.7727097102
138PhosphorylationLGLSSGETDFRLLEE
CCCCCCCHHHHHHHH		43.9227097102
153PhosphorylationSIANLNRSTSVPENP
HHHHCCCCCCCCCCC		24.7823984901
154PhosphorylationIANLNRSTSVPENPK
HHHCCCCCCCCCCCC		30.4223984901
155PhosphorylationANLNRSTSVPENPKS
HHCCCCCCCCCCCCC		36.6021738781
162PhosphorylationSVPENPKSSTSATGC
CCCCCCCCCCCCCCC		39.8525575281
163PhosphorylationVPENPKSSTSATGCA
CCCCCCCCCCCCCCC		32.2925575281
164PhosphorylationPENPKSSTSATGCAT
CCCCCCCCCCCCCCC		29.4925575281
165PhosphorylationENPKSSTSATGCATP
CCCCCCCCCCCCCCC		26.2722108457
167PhosphorylationPKSSTSATGCATPTE
CCCCCCCCCCCCCCC		32.3422668510
171PhosphorylationTSATGCATPTEKEFP
CCCCCCCCCCCCCCC		34.5223712012
173PhosphorylationATGCATPTEKEFPKT
CCCCCCCCCCCCCCC		56.1623984901
180PhosphorylationTEKEFPKTHSDASSE
CCCCCCCCCCCCCHH		27.1822668510
182PhosphorylationKEFPKTHSDASSEQQ
CCCCCCCCCCCHHHH		39.8522668510
186PhosphorylationKTHSDASSEQQNRKS
CCCCCCCHHHHHHHC		40.9422668510
207PhosphorylationGSVKLYPTDQSTFDL
CCEEEEECCHHHHHH		32.8723984901
210PhosphorylationKLYPTDQSTFDLLKD
EEEECCHHHHHHHHH		33.5723984901
211PhosphorylationLYPTDQSTFDLLKDL
EEECCHHHHHHHHHC		18.6123984901
221PhosphorylationLLKDLEFSAGSPSKD
HHHHCCCCCCCCCCC		23.5028432305
224PhosphorylationDLEFSAGSPSKDTNE
HCCCCCCCCCCCCCC		26.229749836
226PhosphorylationEFSAGSPSKDTNESP
CCCCCCCCCCCCCCC		44.8828432305
229PhosphorylationAGSPSKDTNESPWRS
CCCCCCCCCCCCCHH		44.6228432305
232PhosphorylationPSKDTNESPWRSDLL
CCCCCCCCCCHHHCE		31.6519406955
246PhosphorylationLIDENLLSPLAGEDD
EECCCCCCCCCCCCC		22.4919406955
278AcetylationPDTKPKIKDTGDTIL
CCCCCCCCCCCCCCC		56.2022902405
280PhosphorylationTKPKIKDTGDTILSS
CCCCCCCCCCCCCCC		31.7727097102
283PhosphorylationKIKDTGDTILSSPSS
CCCCCCCCCCCCCCC		25.7627097102
286PhosphorylationDTGDTILSSPSSVAL
CCCCCCCCCCCCCCC		36.0321738781
287PhosphorylationTGDTILSSPSSVALP
CCCCCCCCCCCCCCC		25.9229779826
289PhosphorylationDTILSSPSSVALPQV
CCCCCCCCCCCCCCC		39.1227097102
290PhosphorylationTILSSPSSVALPQVK
CCCCCCCCCCCCCCC		18.1327097102
297SumoylationSVALPQVKTEKDDFI
CCCCCCCCCCCCCCH		45.80-
297SumoylationSVALPQVKTEKDDFI
CCCCCCCCCCCCCCH		45.8023508108
313SumoylationLCTPGVIKQEKLGPV
HCCCCCCCHHHCCCE		50.4223508108
313SumoylationLCTPGVIKQEKLGPV
HCCCCCCCHHHCCCE		50.42-
325PhosphorylationGPVYCQASFSGTNII
CCEEEEEEECCCCCC		8.2827097102
327PhosphorylationVYCQASFSGTNIIGN
EEEEEEECCCCCCCC		42.0627097102
390PhosphorylationNWNRCQGSGEDSLTS
CCCCCCCCCCCCCHH		17.0923984901
394PhosphorylationCQGSGEDSLTSLGAL
CCCCCCCCCHHHCCC		29.2827097102
396PhosphorylationGSGEDSLTSLGALNF
CCCCCCCHHHCCCCC		26.3423984901
397PhosphorylationSGEDSLTSLGALNFP
CCCCCCHHHCCCCCC		30.0523984901
407PhosphorylationALNFPGRSVFSNGYS
CCCCCCCCCCCCCCC		33.1728689409
410PhosphorylationFPGRSVFSNGYSSPG
CCCCCCCCCCCCCCC		27.4728689409
413PhosphorylationRSVFSNGYSSPGMRP
CCCCCCCCCCCCCCC		15.3828689409
414PhosphorylationSVFSNGYSSPGMRPD
CCCCCCCCCCCCCCC		31.3028689409
415PhosphorylationVFSNGYSSPGMRPDV
CCCCCCCCCCCCCCC		19.5916641100
423PhosphorylationPGMRPDVSSPPSSSS
CCCCCCCCCCCCCCC		44.5023984901
424PhosphorylationGMRPDVSSPPSSSSA
CCCCCCCCCCCCCCC		40.4528689409
427PhosphorylationPDVSSPPSSSSAATG
CCCCCCCCCCCCCCC		46.3523984901
428PhosphorylationDVSSPPSSSSAATGP
CCCCCCCCCCCCCCC		33.8023984901
429PhosphorylationVSSPPSSSSAATGPP
CCCCCCCCCCCCCCC		28.4723984901
430PhosphorylationSSPPSSSSAATGPPP
CCCCCCCCCCCCCCC		24.7023984901
433PhosphorylationPSSSSAATGPPPKLC
CCCCCCCCCCCCCEE		50.3523984901
448PhosphorylationLVCSDEASGCHYGVL
EEECCCCCCCCEEEE		39.6523984901
452PhosphorylationDEASGCHYGVLTCGS
CCCCCCCEEEEECCC		17.0123984901
456PhosphorylationGCHYGVLTCGSCKVF
CCCEEEEECCCCEEE		16.6523984901
459PhosphorylationYGVLTCGSCKVFFKR
EEEEECCCCEEEEHH		16.9523984901
499AcetylationCPACRYRKCLQAGMN
CCHHHHHHHHHHCCC		30.29-
511AcetylationGMNLEARKTKKKIKG
CCCHHHHHHHHHCCC		72.28-
513AcetylationNLEARKTKKKIKGIQ
CHHHHHHHHHCCCHH		55.92-
514AcetylationLEARKTKKKIKGIQQ
HHHHHHHHHCCCHHH		66.03-
523PhosphorylationIKGIQQATAGVSQDT
CCCHHHHHCCCCCCC		21.4328432305
527PhosphorylationQQATAGVSQDTSENP
HHHHCCCCCCCCCCC		22.7827097102
530PhosphorylationTAGVSQDTSENPNKT
HCCCCCCCCCCCCCC		30.2428432305
531PhosphorylationAGVSQDTSENPNKTI
CCCCCCCCCCCCCCC		43.6028432305
721SumoylationELGKAIVKREGNSSQ
HHHHHHHHHCCCCCH		38.32-
721SumoylationELGKAIVKREGNSSQ
HHHHHHHHHCCCCCH		38.3223508108
Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)
Modified Location Modified Residue Modification Type of Upstream Proteins Gene Name of Upstream Proteins UniProt AC of Upstream Proteins Sources
171TPhosphorylationKinaseGSK3AP49840
PSP
171TPhosphorylationKinaseMAPK1P63086
GPS
171TPhosphorylationKinaseGSK-FAMILY-GPS
224SPhosphorylationKinaseCDK2P24941
PSP
232SPhosphorylationKinaseCDK2P24941
PSP
246SPhosphorylationKinaseMAPK1P63086
GPS
246SPhosphorylationKinaseMAPK8P45983
GPS
246SPhosphorylationKinaseJNK1P49185
PSP
246SPhosphorylationKinaseMAPK9P45984
GPS
246SPhosphorylationKinaseMAPK10P53779
GPS
527SPhosphorylationKinasePRKDCP78527
GPS
Functions of PTM Sites
Modified Location Modified Residue Modification Function Reference
224SPhosphorylation

9603939
224SPhosphorylation

9603939
224SPhosphorylation

9603939
232SPhosphorylation

9603939
232SPhosphorylation

9603939
232SPhosphorylation

9603939
246SPhosphorylation

9603939
246SPhosphorylation

9603939
297KSumoylation

23508108
297KSumoylation

23508108
313KSumoylation

23508108
313KSumoylation

23508108
424SPhosphorylation

-
424SPhosphorylation

-
424SPhosphorylation

-
721KSumoylation

23508108
721KSumoylation

23508108
Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location Modification Variant Position
(Distance <= 10) 		Residue Change SAP Related Disease Reference

Oops, there are no SNP-PTM records of GCR_RAT !!
Protein-Protein Interaction
Interacting Protein Gene Name Interaction Type PPI Reference Domain-Domain Interactions
MAS5_YEASTYDJ1physical
18829866
HSP77_YEASTSSC1physical
18829866
HSC82_YEASTHSC82physical
18829866
HSP82_YEASTHSP82physical
18829866
HS90A_RATHsp90aa1physical
12145311
HSP74_RATHspa4physical
12145311
UBC_RATUbcphysical
24334056
Drug and Disease Associations
Kegg Drug
DrugBank
There are no disease associations of PTM sites.
Regulatory Network of GCR_RAT

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Related Literatures of Post-Translational Modification
Phosphorylation
ReferencePubMed
"Phosphorylation and inhibition of rat glucocorticoid receptortranscriptional activation by glycogen synthase kinase-3 (GSK-3).Species-specific differences between human and rat glucocorticoidreceptor signaling as revealed through GSK-3 phosphorylation.";
Rogatsky I., Waase C.L.M., Garabedian M.J.;
J. Biol. Chem. 273:14315-14321(1998).
Cited for: PHOSPHORYLATION AT THR-171; SER-224; SER-232 AND SER-246.

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