GATA4_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: GATA4_MOUSE
• UniProt AC: Q08369
• Protein Name: Transcription factor GATA-4
• Gene Name: Gata4
• Organism: Mus musculus (Mouse).
• Sequence Length: 441
• Subcellular Localization: Nucleus .
• Protein Description: Transcriptional activator that binds to the consensus sequence 5'-AGATAG-3' and plays a key role in cardiac development (By similarity). In cooperation with TBX5, it binds to cardiac super-enhancers and promotes cardiomyocyte gene expression, while it downregulates endocardial and endothelial gene expression (By similarity). Involved in bone morphogenetic protein (BMP)-mediated induction of cardiac-specific gene expression (By similarity). Binds to BMP response element (BMPRE) DNA sequences within cardiac activating regions (By similarity). Acts as a transcriptional activator of ANF in cooperation with NKX2-5. [PubMed: 9584153 Promotes cardiac myocyte enlargement (By similarity Required during testicular development (By similarity May play a role in sphingolipid signaling by regulating the expression of sphingosine-1-phosphate degrading enzyme, spingosine-1-phosphate lyase (By similarity]
• Protein Sequence: MYQSLAMAANHGPPPGAYEAGGPGAFMHSAGAASSPVYVPTPRVPSSVLGLSYLQGGGSAAAAGTTSGGSSGAGPSGAGPGTQQGSPGWSQAGAEGAAYTPPPVSPRFSFPGTTGSLAAAAAAAAAREAAAYGSGGGAAGAGLAGREQYGRPGFAGSYSSPYPAYMADVGASWAAAAAASAGPFDSPVLHSLPGRANPGRHPNLDMFDDFSEGRECVNCGAMSTPLWRRDGTGHYLCNACGLYHKMNGINRPLIKPQRRLSASRRVGLSCANCQTTTTTLWRRNAEGEPVCNACGLYMKLHGVPRPLAMRKEGIQTRKRKPKNLNKSKTPAGPAGETLPPSSGASSGNSSNATSSSSSSEEMRPIKTEPGLSSHYGHSSSMSQTFSTVSGHGPSIHPVLSALKLSPQGYASPVTQTSQASSKQDSWNSLVLADSHGDIITA

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
18	Phosphorylation	HGPPPGAYEAGGPGA CCCCCCCHHCCCCCH	16.54	26643407
29	Phosphorylation	GPGAFMHSAGAASSP CCCHHCCCCCCCCCC	19.33	26643407
34	Phosphorylation	MHSAGAASSPVYVPT CCCCCCCCCCEECCC	34.34	26643407
35	Phosphorylation	HSAGAASSPVYVPTP CCCCCCCCCEECCCC	17.41	26643407
38	Phosphorylation	GAASSPVYVPTPRVP CCCCCCEECCCCCCC	12.23	26643407
41	Phosphorylation	SSPVYVPTPRVPSSV CCCEECCCCCCCHHH	17.43	26643407
105	Phosphorylation	AYTPPPVSPRFSFPG CCCCCCCCCCCCCCC	19.01	21746915
109	Phosphorylation	PPVSPRFSFPGTTGS CCCCCCCCCCCCHHH	31.63	26643407
134	Phosphorylation	REAAAYGSGGGAAGA HHHHHHCCCCCCCCC	22.91	28066266
160	Phosphorylation	GFAGSYSSPYPAYMA CCCCCCCCCCCCCCH	21.88	-
261	Phosphorylation	IKPQRRLSASRRVGL CCHHHHHCHHHHHCC	23.53	23140645
275	Phosphorylation	LSCANCQTTTTTLWR CCCCCCCCCCCCCCC	28.45	28576409
299	Methylation	NACGLYMKLHGVPRP HHHHHHHHHHCCCCC	24.63	22215809
311	Acetylation	PRPLAMRKEGIQTRK CCCHHHHHHHHHCCC	47.40	36761605
318	Acetylation	KEGIQTRKRKPKNLN HHHHHCCCCCCCCCC	69.07	36761617
320	Acetylation	GIQTRKRKPKNLNKS HHHCCCCCCCCCCCC	64.48	36761611
322	Acetylation	QTRKRKPKNLNKSKT HCCCCCCCCCCCCCC	76.68	36761599
366	Sumoylation	SEEMRPIKTEPGLSS CCCCCCCCCCCCCCC	49.90	-
367	Phosphorylation	EEMRPIKTEPGLSSH CCCCCCCCCCCCCCC	48.40	-
405	Phosphorylation	VLSALKLSPQGYASP HHHHHCCCCCCCCCC	41.89	27087446
409	Phosphorylation	LKLSPQGYASPVTQT HCCCCCCCCCCCCCC	17.74	29472430
411	Phosphorylation	LSPQGYASPVTQTSQ CCCCCCCCCCCCCCC	25.63	28066266
414	Phosphorylation	QGYASPVTQTSQASS CCCCCCCCCCCCCCC	39.11	28066266
416	Phosphorylation	YASPVTQTSQASSKQ CCCCCCCCCCCCCCC	17.58	28066266
417	Phosphorylation	ASPVTQTSQASSKQD CCCCCCCCCCCCCCC	48.96	29472430

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
105	S	Phosphorylation	Kinase	MAPK1	P63085	GPS
105	S	Phosphorylation	Kinase	ERK1	P27361	PSP
160	S	Phosphorylation	Kinase	CDK4	P11802	PSP
261	S	Phosphorylation	Kinase	MAPK1	P28482	GPS
261	S	Phosphorylation	Kinase	MAPK3	P27361	GPS
-	K	Ubiquitination	E3 ubiquitin ligase	Stub1	Q9WUD1	PMID:22199232

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
299	K	Methylation	Methylation at Lys-299 attenuates transcriptional activity.	22215809

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of GATA4_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
MED1_MOUSE	Med1	physical	20211142
SMAD1_MOUSE	Smad1	physical	14662776
SMAD2_MOUSE	Smad2	physical	14662776
SMAD3_MOUSE	Smad3	physical	14662776
SMAD4_MOUSE	Smad4	physical	14662776
SMCA4_MOUSE	Smarca4	physical	15525990
SMRD3_MOUSE	Smarcd3	physical	15525990
EP300_MOUSE	Ep300	physical	11481322
CDK9_HUMAN	CDK9	physical	20081228
CCNT1_HUMAN	CCNT1	physical	20081228
EP300_HUMAN	EP300	physical	20081228
ACTB_HUMAN	ACTB	physical	20081228
FAK1_MOUSE	Ptk2	physical	22734001
FOG2_MOUSE	Zfpm2	physical	17445768

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Methylation

"PRC2 directly methylates GATA4 and represses its transcriptionalactivity.";
He A., Shen X., Ma Q., Cao J., von Gise A., Zhou P., Wang G.,Marquez V.E., Orkin S.H., Pu W.T.;
Genes Dev. 26:37-42(2012).
Cited for: METHYLATION AT LYS-299.
PubMed: 22215809