dbPTM

FA12_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	FA12_HUMAN
UniProt AC	P00748
Protein Name	Coagulation factor XII
Gene Name	F12
Organism	Homo sapiens (Human).
Sequence Length	615
Subcellular Localization	Secreted.
Protein Description	Factor XII is a serum glycoprotein that participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then trypsin cleaves it to beta-factor XIIa. Alpha-factor XIIa activates factor XI to factor XIa..
Protein Sequence	MRALLLLGFLLVSLESTLSIPPWEAPKEHKYKAEEHTVVLTVTGEPCHFPFQYHRQLYHKCTHKGRPGPQPWCATTPNFDQDQRWGYCLEPKKVKDHCSKHSPCQKGGTCVNMPSGPHCLCPQHLTGNHCQKEKCFEPQLLRFFHKNEIWYRTEQAAVARCQCKGPDAHCQRLASQACRTNPCLHGGRCLEVEGHRLCHCPVGYTGAFCDVDTKASCYDGRGLSYRGLARTTLSGAPCQPWASEATYRNVTAEQARNWGLGGHAFCRNPDNDIRPWCFVLNRDRLSWEYCDLAQCQTPTQAAPPTPVSPRLHVPLMPAQPAPPKPQPTTRTPPQSQTPGALPAKREQPPSLTRNGPLSCGQRLRKSLSSMTRVVGGLVALRGAHPYIAALYWGHSFCAGSLIAPCWVLTAAHCLQDRPAPEDLTVVLGQERRNHSCEPCQTLAVRSYRLHEAFSPVSYQHDLALLRLQEDADGSCALLSPYVQPVCLPSGAARPSETTLCQVAGWGHQFEGAEEYASFLQEAQVPFLSLERCSAPDVHGSSILPGMLCAGFLEGGTDACQGDSGGPLVCEDQAAERRLTLQGIISWGSGCGDRNKPGVYTDVAYYLAWIREHTVS

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
31	Phosphorylation	EAPKEHKYKAEEHTV CCCCCCCCCCCCCEE	19.82	-
43	O-linked_Glycosylation	HTVVLTVTGEPCHFP CEEEEEECCCCCCCC	30.50	OGP
53	Phosphorylation	PCHFPFQYHRQLYHK CCCCCHHHHHHHHHH	10.19	-
58	Phosphorylation	FQYHRQLYHKCTHKG HHHHHHHHHHCCCCC	7.30	-
109	O-linked_Glycosylation	SPCQKGGTCVNMPSG CCCCCCCEEECCCCC	23.19	1544894
231	Phosphorylation	SYRGLARTTLSGAPC CHHCEEEECCCCCCC	26.87	24719451
243	Phosphorylation	APCQPWASEATYRNV CCCCCCCCCCCCCCC	24.72	-
246	Phosphorylation	QPWASEATYRNVTAE CCCCCCCCCCCCCHH	20.97	24719451
249	N-linked_Glycosylation	ASEATYRNVTAEQAR CCCCCCCCCCHHHHH	25.69	3886654
297	O-linked_Glycosylation	CDLAQCQTPTQAAPP CCHHHCCCCCCCCCC	35.61	OGP
299	O-linked_Glycosylation	LAQCQTPTQAAPPTP HHHCCCCCCCCCCCC	33.94	3886654
305	O-linked_Glycosylation	PTQAAPPTPVSPRLH CCCCCCCCCCCCCCC	34.95	3886654
308	O-linked_Glycosylation	AAPPTPVSPRLHVPL CCCCCCCCCCCCCCC	12.90	3886654
308	Phosphorylation	AAPPTPVSPRLHVPL CCCCCCCCCCCCCCC	12.90	24945436
328	O-linked_Glycosylation	APPKPQPTTRTPPQS CCCCCCCCCCCCCCC	24.54	3886654
329	O-linked_Glycosylation	PPKPQPTTRTPPQSQ CCCCCCCCCCCCCCC	38.95	3886654
331	O-linked_Glycosylation	KPQPTTRTPPQSQTP CCCCCCCCCCCCCCC	36.91	OGP
331	Phosphorylation	KPQPTTRTPPQSQTP CCCCCCCCCCCCCCC	36.91	-
335	O-linked_Glycosylation	TTRTPPQSQTPGALP CCCCCCCCCCCCCCC	41.62	OGP
337	O-linked_Glycosylation	RTPPQSQTPGALPAK CCCCCCCCCCCCCCC	29.01	3886654
350	Phosphorylation	AKREQPPSLTRNGPL CCCCCCCCCCCCCCC	49.40	24505115
366	Phosphorylation	CGQRLRKSLSSMTRV HHHHHHHHHHHHHHH	27.35	-
368	Phosphorylation	QRLRKSLSSMTRVVG HHHHHHHHHHHHHHH	25.75	-
371	Phosphorylation	RKSLSSMTRVVGGLV HHHHHHHHHHHHHHH	23.43	-
433	N-linked_Glycosylation	VLGQERRNHSCEPCQ EECCCCCCCCCCCCC	37.33	17623646
433	N-linked_Glycosylation	VLGQERRNHSCEPCQ EECCCCCCCCCCCCC	37.33	16335952
466	Methylation	QHDLALLRLQEDADG HHHHHHHEECCCCCC	34.86	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
Oops, there are no upstream regulatory protein records of FA12_HUMAN !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of FA12_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of FA12_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
EPAS1_HUMAN	EPAS1	physical	21512133
PJA1_HUMAN	PJA1	physical	26186194
AMRA1_HUMAN	AMBRA1	physical	26186194
NUDC1_HUMAN	NUDCD1	physical	26186194
MRRP3_HUMAN	KIAA0391	physical	26186194
HIF1A_HUMAN	HIF1A	physical	18838541
FA12_HUMAN	F12	physical	18838541
ANR40_HUMAN	ANKRD40	physical	28514442
HSP7C_HUMAN	HSPA8	physical	28514442

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
234000	Factor XII deficiency (FA12D)
610618	Hereditary angioedema 3 (HAE3)
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of FA12_HUMAN

Related Literatures of Post-Translational Modification

N-linked Glycosylation
Reference	PubMed
"Human plasma N-glycoproteome analysis by immunoaffinity subtraction,hydrazide chemistry, and mass spectrometry."; Liu T., Qian W.-J., Gritsenko M.A., Camp D.G. II, Monroe M.E.,Moore R.J., Smith R.D.; J. Proteome Res. 4:2070-2080(2005). Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-249 AND ASN-433, AND MASSSPECTROMETRY.	16335952 [Abstract]
"Screening for N-glycosylated proteins by liquid chromatography massspectrometry."; Bunkenborg J., Pilch B.J., Podtelejnikov A.V., Wisniewski J.R.; Proteomics 4:454-465(2004). Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-433, AND MASSSPECTROMETRY.	14760718 [Abstract]
"Amino acid sequence of the heavy chain of human alpha-factor XIIa(activated Hageman factor)."; McMullen B.A., Fujikawa K.; J. Biol. Chem. 260:5328-5341(1985). Cited for: PROTEIN SEQUENCE OF 20-379, GLYCOSYLATION AT ASN-249; THR-299;THR-305; SER-308; THR-328; THR-329 AND THR-337, AND VARIANT PRO-207.	3886654 [Abstract]
"Characterization of human blood coagulation factor XII cDNA.Prediction of the primary structure of factor XII and the tertiarystructure of beta-factor XIIa."; Cool D.E., Edgell C.-J.S., Louie G.V., Zoller M.J., Brayer G.D.,McGillivray R.T.A.; J. Biol. Chem. 260:13666-13676(1985). Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 14-615, AND VARIANT PRO-207.	3877053 [Abstract]
O-linked Glycosylation
Reference	PubMed
"O-linked fucose is present in the first epidermal growth factordomain of factor XII but not protein C."; Harris R.J., Ling V.T., Spellman M.W.; J. Biol. Chem. 267:5102-5107(1992). Cited for: GLYCOSYLATION AT THR-109.	1544894 [Abstract]
"Amino acid sequence of the heavy chain of human alpha-factor XIIa(activated Hageman factor)."; McMullen B.A., Fujikawa K.; J. Biol. Chem. 260:5328-5341(1985). Cited for: PROTEIN SEQUENCE OF 20-379, GLYCOSYLATION AT ASN-249; THR-299;THR-305; SER-308; THR-328; THR-329 AND THR-337, AND VARIANT PRO-207.	3886654 [Abstract]