dbPTM

EPS15_MOUSE - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	EPS15_MOUSE
UniProt AC	P42567
Protein Name	Epidermal growth factor receptor substrate 15
Gene Name	Eps15
Organism	Mus musculus (Mouse).
Sequence Length	897
Subcellular Localization	Cytoplasm. Cell membrane Peripheral membrane protein Cytoplasmic side. Membrane, clathrin-coated pit. Recruited to the plasma membrane upon EGFR activation and localizes to coated pits. Colocalizes with UBQLN1 in ubiquitin-rich cytoplasmic aggregat
Protein Description	Involved in cell growth regulation. May be involved in the regulation of mitogenic signals and control of cell proliferation. Involved in the internalization of ligand-inducible receptors of the receptor tyrosine kinase (RTK) type, in particular EGFR. Plays a role in the assembly of clathrin-coated pits (CCPs). Acts as a clathrin adapter required for post-Golgi trafficking. Seems to be involved in CCPs maturation including invagination or budding. Involved in endocytosis of integrin beta-1 (ITGB1) and transferrin receptor (TFR); internalization of ITGB1 as DAB2-dependent cargo but not TFR seems to require association with DAB2..
Protein Sequence	MAAAAQLSLTQLSSGNPVYEKYYRQVEAGNTGRVLALDAAAFLKKSGLPDLILGKIWDLADTDGKGVLSKQEFFVALRLVACAQNGLEVSLSSLSLAVPPPRFHDSSSPLLTSGPSVAELPWAVKSEDKAKYDAIFDSLSPVDGFLSGDKVKPVLLNSKLPVEILGRVWELSDIDHDGKLDRDEFAVAMFLVYCALEKEPVPMSLPPALVPPSKRKTWVVSPAEKAKYDEIFLKTDKDMDGYVSGLEVRETFLKTGLPSALLAHIWSLCDTKGCGKLSKDQFALAFHLINQKLIKGIDPPHSLTPEMIPPSDRSSLQKNITGSSPVADFSAIKELDTLNNEIVDLQREKNNVEQDLKEKEDTVKQRTSEVQDLQDEVQRESINLQKLQAQKQQVQELLGELDEQKAQLEEQLQEVRKKCAEEAQLISSLKAEITSQESQISSYEEELLKAREELSRLQQETAQLEESVESGKAQLEPLQQHLQESQQEISSMQMRLEMKDLETDNNQSNWSSSPQSVLVNGATDYCSLSTSSSETANFNEHAEGQNNLESEPTHQESSVRSSPEIAPSDVTDESEAVTVAGNEKVTPRFDDDKHSKEEDPFNVESSSLTDAVADTNLDFFQSDPFVGSDPFKDDPFGKIDPFGGDPFKGSDPFASDCFFKQTSTDPFTTSSTDPFSASSNSSNTSVETWKHNDPFAPGGTVVAAASDSATDPFASVFGNESFGDGFADFSTLSKVNNEDAFNPTISSSTSSVTIAKPMLEETASKSEDVPPALPPKVGTPTRPCPPPPGKRPINKLDSSDPLKLNDPFQPFPGNDSPKEKDPDMFCDPFTSSTTTNKEADPSNFANFSAYPSEEDMIEWAKRESEREEEQRLARLNQQEQEDLELAIALSKSEISEA

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Acetylation	------MAAAAQLSL ------CCHHHHHHH	13.05	-
8	Phosphorylation	MAAAAQLSLTQLSSG CCHHHHHHHHHHHCC	19.79	30352176
10	Phosphorylation	AAAQLSLTQLSSGNP HHHHHHHHHHHCCCC	24.88	28066266
13	Phosphorylation	QLSLTQLSSGNPVYE HHHHHHHHCCCCHHH	26.78	25293948
14	Phosphorylation	LSLTQLSSGNPVYEK HHHHHHHCCCCHHHH	51.70	25293948
19	Phosphorylation	LSSGNPVYEKYYRQV HHCCCCHHHHHHHHH	14.14	25293948
106	Phosphorylation	PPPRFHDSSSPLLTS CCCCCCCCCCCCCCC	24.93	24925903
107	Phosphorylation	PPRFHDSSSPLLTSG CCCCCCCCCCCCCCC	41.87	24925903
108	Phosphorylation	PRFHDSSSPLLTSGP CCCCCCCCCCCCCCC	24.42	24925903
112	Phosphorylation	DSSSPLLTSGPSVAE CCCCCCCCCCCCHHH	39.04	24925903
113	Phosphorylation	SSSPLLTSGPSVAEL CCCCCCCCCCCHHHC	48.99	24925903
116	Phosphorylation	PLLTSGPSVAELPWA CCCCCCCCHHHCCCE	37.17	24925903
138	Phosphorylation	KYDAIFDSLSPVDGF HHHHHHHCCCCCCCC	21.72	23984901
140	Phosphorylation	DAIFDSLSPVDGFLS HHHHHCCCCCCCCCC	27.16	26745281
221	Phosphorylation	KRKTWVVSPAEKAKY CCCCEEECHHHHCCC	14.58	25338131
227	Ubiquitination	VSPAEKAKYDEIFLK ECHHHHCCCCEEEEE	65.39	22790023
242	Phosphorylation	TDKDMDGYVSGLEVR CCCCCCCCCCCHHHH	6.28	17242355
244	Phosphorylation	KDMDGYVSGLEVRET CCCCCCCCCHHHHHH	29.22	26525534
321	Phosphorylation	SSLQKNITGSSPVAD HHHHHHCCCCCCCCC	40.17	24925903
323	Phosphorylation	LQKNITGSSPVADFS HHHHCCCCCCCCCHH	23.87	24925903
324	Phosphorylation	QKNITGSSPVADFSA HHHCCCCCCCCCHHH	25.41	24925903
330	Phosphorylation	SSPVADFSAIKELDT CCCCCCHHHHHHHHH	29.63	28833060
337	Phosphorylation	SAIKELDTLNNEIVD HHHHHHHHHCHHHHH	43.97	25367039
367	Phosphorylation	EDTVKQRTSEVQDLQ HHHHHHHHHHHHHHH	27.09	25338131
386	Ubiquitination	RESINLQKLQAQKQQ HHHHHHHHHHHHHHH	45.81	22790023
434	Phosphorylation	SSLKAEITSQESQIS HHHHHHHHCCHHHHH	18.83	30635358
435	Phosphorylation	SLKAEITSQESQISS HHHHHHHCCHHHHHH	37.30	30635358
438	Phosphorylation	AEITSQESQISSYEE HHHHCCHHHHHHHHH	25.77	30635358
441	Phosphorylation	TSQESQISSYEEELL HCCHHHHHHHHHHHH	21.16	30635358
442	Phosphorylation	SQESQISSYEEELLK CCHHHHHHHHHHHHH	37.89	30635358
443	Phosphorylation	QESQISSYEEELLKA CHHHHHHHHHHHHHH	22.14	30635358
449	Ubiquitination	SYEEELLKAREELSR HHHHHHHHHHHHHHH	59.01	22790023
467	Phosphorylation	ETAQLEESVESGKAQ HHHHHHHHHHHCHHH	23.08	-
470	Phosphorylation	QLEESVESGKAQLEP HHHHHHHHCHHHHHH	43.36	-
472	Ubiquitination	EESVESGKAQLEPLQ HHHHHHCHHHHHHHH	42.09	22790023
485	Phosphorylation	LQQHLQESQQEISSM HHHHHHHHHHHHHHH	24.97	-
561	Phosphorylation	HQESSVRSSPEIAPS CCCCCCCCCCCCCCC	48.35	25521595
562	Phosphorylation	QESSVRSSPEIAPSD CCCCCCCCCCCCCCC	18.91	25521595
568	Phosphorylation	SSPEIAPSDVTDESE CCCCCCCCCCCCCCC	35.37	25619855
571	Phosphorylation	EIAPSDVTDESEAVT CCCCCCCCCCCCCEE	38.75	25619855
574	Phosphorylation	PSDVTDESEAVTVAG CCCCCCCCCCEEECC	33.40	25619855
578	Phosphorylation	TDESEAVTVAGNEKV CCCCCCEEECCCCEE	16.22	25619855
586	Phosphorylation	VAGNEKVTPRFDDDK ECCCCEECCCCCCCC	21.58	25619855
595	Phosphorylation	RFDDDKHSKEEDPFN CCCCCCCCCCCCCCC	47.72	23375375
605	Phosphorylation	EDPFNVESSSLTDAV CCCCCCCCCHHHHHH	22.42	23737553
606	Phosphorylation	DPFNVESSSLTDAVA CCCCCCCCHHHHHHH	18.83	23737553
607	Phosphorylation	PFNVESSSLTDAVAD CCCCCCCHHHHHHHC	44.56	23737553
609	Phosphorylation	NVESSSLTDAVADTN CCCCCHHHHHHHCCC	24.70	23737553
615	Phosphorylation	LTDAVADTNLDFFQS HHHHHHCCCCCHHHC	28.13	26643407
622	Phosphorylation	TNLDFFQSDPFVGSD CCCCHHHCCCCCCCC	41.94	26643407
638	Ubiquitination	FKDDPFGKIDPFGGD CCCCCCCCCCCCCCC	44.29	22790023
648	Ubiquitination	PFGGDPFKGSDPFAS CCCCCCCCCCCCCCC	63.99	-
655	Phosphorylation	KGSDPFASDCFFKQT CCCCCCCCCCCEEEC	34.98	28066266
662	Phosphorylation	SDCFFKQTSTDPFTT CCCCEEECCCCCCCC	33.90	25338131
664	Phosphorylation	CFFKQTSTDPFTTSS CCEEECCCCCCCCCC	51.59	25338131
670	Phosphorylation	STDPFTTSSTDPFSA CCCCCCCCCCCCCCC	28.34	25338131
681	Phosphorylation	PFSASSNSSNTSVET CCCCCCCCCCCCEEE	27.24	25338131
700	Phosphorylation	DPFAPGGTVVAAASD CCCCCCCEEEEEECC	20.09	26643407
706	Phosphorylation	GTVVAAASDSATDPF CEEEEEECCCCCCCC	27.63	26643407
708	Phosphorylation	VVAAASDSATDPFAS EEEEECCCCCCCCHH	30.49	26643407
710	Phosphorylation	AAASDSATDPFASVF EEECCCCCCCCHHHH	48.43	26643407
715	Phosphorylation	SATDPFASVFGNESF CCCCCCHHHHCCCCC	20.54	26643407
721	Phosphorylation	ASVFGNESFGDGFAD HHHHCCCCCCCCCCC	38.23	26643407
730	Phosphorylation	GDGFADFSTLSKVNN CCCCCCHHHHHCCCC	29.05	26643407
731	Phosphorylation	DGFADFSTLSKVNNE CCCCCHHHHHCCCCH	34.74	26643407
733	Phosphorylation	FADFSTLSKVNNEDA CCCHHHHHCCCCHHH	34.35	26643407
744	Phosphorylation	NEDAFNPTISSSTSS CHHHCCCCCCCCCCC	34.30	25293948
746	Phosphorylation	DAFNPTISSSTSSVT HHCCCCCCCCCCCEE	22.25	25293948
747	Phosphorylation	AFNPTISSSTSSVTI HCCCCCCCCCCCEEE	33.51	25293948
748	Phosphorylation	FNPTISSSTSSVTIA CCCCCCCCCCCEEEE	26.05	25293948
749	Phosphorylation	NPTISSSTSSVTIAK CCCCCCCCCCEEEEH	27.28	25293948
750	Phosphorylation	PTISSSTSSVTIAKP CCCCCCCCCEEEEHH	25.43	29472430
751	Phosphorylation	TISSSTSSVTIAKPM CCCCCCCCEEEEHHH	24.30	25293948
753	Phosphorylation	SSSTSSVTIAKPMLE CCCCCCEEEEHHHHH	19.71	25293948
762	Phosphorylation	AKPMLEETASKSEDV EHHHHHHHHCCCCCC	27.19	25293948
764	Phosphorylation	PMLEETASKSEDVPP HHHHHHHCCCCCCCC	44.10	30352176
766	Phosphorylation	LEETASKSEDVPPAL HHHHHCCCCCCCCCC	36.21	25338131
779	Phosphorylation	ALPPKVGTPTRPCPP CCCCCCCCCCCCCCC	25.19	26824392
781	Phosphorylation	PPKVGTPTRPCPPPP CCCCCCCCCCCCCCC	47.26	28725479
795	Ubiquitination	PGKRPINKLDSSDPL CCCCCCCCCCCCCCC	55.16	22790023
798	Phosphorylation	RPINKLDSSDPLKLN CCCCCCCCCCCCCCC	47.26	26824392
799	Phosphorylation	PINKLDSSDPLKLND CCCCCCCCCCCCCCC	42.80	22942356
803	Ubiquitination	LDSSDPLKLNDPFQP CCCCCCCCCCCCCCC	51.11	22790023
816	Phosphorylation	QPFPGNDSPKEKDPD CCCCCCCCCCCCCCC	41.70	24925903
818	Ubiquitination	FPGNDSPKEKDPDMF CCCCCCCCCCCCCCC	79.80	22790023
820	Ubiquitination	GNDSPKEKDPDMFCD CCCCCCCCCCCCCCC	79.34	22790023
830	Phosphorylation	DMFCDPFTSSTTTNK CCCCCCCCCCCCCCC	27.44	23984901
831	Phosphorylation	MFCDPFTSSTTTNKE CCCCCCCCCCCCCCC	26.51	23984901
832	Phosphorylation	FCDPFTSSTTTNKEA CCCCCCCCCCCCCCC	27.07	23984901
833	Phosphorylation	CDPFTSSTTTNKEAD CCCCCCCCCCCCCCC	37.09	23984901
842	Phosphorylation	TNKEADPSNFANFSA CCCCCCCCCCCCCCC	45.16	23984901
848	Phosphorylation	PSNFANFSAYPSEED CCCCCCCCCCCCHHH	27.05	23984901
850	Phosphorylation	NFANFSAYPSEEDMI CCCCCCCCCCHHHHH	13.19	18515860
852	Phosphorylation	ANFSAYPSEEDMIEW CCCCCCCCHHHHHHH	40.73	27180971
864	Phosphorylation	IEWAKRESEREEEQR HHHHHHHHHHHHHHH	46.09	20469934

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
850	Y	Phosphorylation	Kinase	EGFR	P00533	PSP
850	Y	Phosphorylation	Kinase	EGFR	Q01279	Uniprot

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of EPS15_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of EPS15_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
DYN1_HUMAN	DNM1	physical	11483962
EPS15_MOUSE	Eps15	physical	9182572
AGFG1_HUMAN	AGFG1	physical	9303539
NUMB_HUMAN	NUMB	physical	9303539
NUMBL_HUMAN	NUMBL	physical	9303539
PRP17_HUMAN	CDC40	physical	9303539
EPN2_HUMAN	EPN2	physical	9303539
AGFG2_HUMAN	AGFG2	physical	9303539
PRKN_HUMAN	PARK2	physical	16862145
PRKN_MOUSE	Park2	physical	20064468
ITSN1_MOUSE	Itsn1	physical	10064583

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of EPS15_MOUSE

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"The phagosomal proteome in interferon-gamma-activated macrophages."; Trost M., English L., Lemieux S., Courcelles M., Desjardins M.,Thibault P.; Immunity 30:143-154(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-779 AND SER-816, ANDMASS SPECTROMETRY.	19144319 [Abstract]
"Large-scale phosphorylation analysis of mouse liver."; Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.; Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-106; SER-324; SER-561;SER-562 AND THR-781, AND MASS SPECTROMETRY.	17242355 [Abstract]
"Tyrosine phosphorylation of Eps15 is required for ligand-regulated,but not constitutive, endocytosis."; Confalonieri S., Salcini A.E., Puri C., Tacchetti C., Di Fiore P.P.; J. Cell Biol. 150:905-912(2000). Cited for: PHOSPHORYLATION AT TYR-850 BY EGFR, AND MUTAGENESIS OF TYR-850.	10953014 [Abstract]