DTX1_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: DTX1_HUMAN
• UniProt AC: Q86Y01
• Protein Name: E3 ubiquitin-protein ligase DTX1
• Gene Name: DTX1
• Organism: Homo sapiens (Human).
• Sequence Length: 620
• Subcellular Localization: Cytoplasm. Nucleus. Predominantly cytoplasmic. Associates with endocytic vesicles. Partially nuclear.
• Protein Description: Functions as a ubiquitin ligase protein in vivo, mediating ubiquitination and promoting degradation of MEKK1, suggesting that it may regulate the Notch pathway via some ubiquitin ligase activity (By similarity). Regulator of Notch signaling, a signaling pathway involved in cell-cell communications that regulates a broad spectrum of cell-fate determinations. Mainly acts as a positive regulator of Notch, but it also acts as a negative regulator, depending on the developmental and cell context. Mediates the antineural activity of Notch, possibly by inhibiting the transcriptional activation mediated by MATCH1. Involved in neurogenesis, lymphogenesis and myogenesis, and may also be involved in MZB (Marginal zone B) cell differentiation. Promotes B-cell development at the expense of T-cell development, suggesting that it can antagonize NOTCH1..
• Protein Sequence: MSRPGHGGLMPVNGLGFPPQNVARVVVWEWLNEHSRWRPYTATVCHHIENVLKEDARGSVVLGQVDAQLVPYIIDLQSMHQFRQDTGTMRPVRRNFYDPSSAPGKGIVWEWENDGGAWTAYDMDICITIQNAYEKQHPWLDLSSLGFCYLIYFNSMSQMNRQTRRRRRLRRRLDLAYPLTVGSIPKSQSWPVGASSGQPCSCQQCLLVNSTRAASNAILASQRRKAPPAPPLPPPPPPGGPPGALAVRPSATFTGAALWAAPAAGPAEPAPPPGAPPRSPGAPGGARTPGQNNLNRPGPQRTTSVSARASIPPGVPALPVKNLNGTGPVHPALAGMTGILLCAAGLPVCLTRAPKPILHPPPVSKSDVKPVPGVPGVCRKTKKKHLKKSKNPEDVVRRYMQKVKNPPDEDCTICMERLVTASGYEGVLRHKGVRPELVGRLGRCGHMYHLLCLVAMYSNGNKDGSLQCPTCKAIYGEKTGTQPPGKMEFHLIPHSLPGFPDTQTIRIVYDIPTGIQGPEHPNPGKKFTARGFPRHCYLPNNEKGRKVLRLLITAWERRLIFTIGTSNTTGESDTVVWNEIHHKTEFGSNLTGHGYPDASYLDNVLAELTAQGVSEAAAKA

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
187	Phosphorylation	TVGSIPKSQSWPVGA CCCCCCHHHCCCCCC	24.88	26074081
189	Phosphorylation	GSIPKSQSWPVGASS CCCCHHHCCCCCCCC	40.20	26074081
195	Phosphorylation	QSWPVGASSGQPCSC HCCCCCCCCCCCCCC	29.13	26074081
196	Phosphorylation	SWPVGASSGQPCSCQ CCCCCCCCCCCCCCC	40.70	26074081
201	Phosphorylation	ASSGQPCSCQQCLLV CCCCCCCCCCEEEEC	22.95	26074081
210	Phosphorylation	QQCLLVNSTRAASNA CEEEECCCHHHHHHH	16.28	24719451
211	Phosphorylation	QCLLVNSTRAASNAI EEEECCCHHHHHHHH	21.08	26074081
215	Phosphorylation	VNSTRAASNAILASQ CCCHHHHHHHHHHHH	26.30	28122231
221	Phosphorylation	ASNAILASQRRKAPP HHHHHHHHHHCCCCC	22.30	24850871
279	Phosphorylation	PPGAPPRSPGAPGGA CCCCCCCCCCCCCCC	33.20	26074081
288	Phosphorylation	GAPGGARTPGQNNLN CCCCCCCCCCCCCCC	31.10	26074081
303	Phosphorylation	RPGPQRTTSVSARAS CCCCCCCCCCEECCC	29.35	25954137
310	Phosphorylation	TSVSARASIPPGVPA CCCEECCCCCCCCCC	30.03	28111955
355	Ubiquitination	VCLTRAPKPILHPPP EEECCCCCCCCCCCC	43.64	29967540
389	Phosphorylation	KKKHLKKSKNPEDVV HHHHHHCCCCHHHHH	35.76	23532336
404	Ubiquitination	RRYMQKVKNPPDEDC HHHHHHHCCCCCCCC	71.43	-
424	Phosphorylation	RLVTASGYEGVLRHK HHHHCCCHHHHHHCC	13.65	21214269
431	Ubiquitination	YEGVLRHKGVRPELV HHHHHHCCCCCHHHH	53.30	-
472	Ubiquitination	SLQCPTCKAIYGEKT CCCCCCCCCCCCCCC	40.05	29967540
526	Ubiquitination	EHPNPGKKFTARGFP CCCCCCCCCCCCCCC	54.59	-
619	Ubiquitination	GVSEAAAKA------ CHHHHHHHC------	50.32	29967540

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
-	K	Ubiquitination	E3 ubiquitin ligase	MAP3K1	Q13233	PMID:15684388
-	K	Ubiquitination	E3 ubiquitin ligase	ITCH	Q96J02	PMID:22199232
-	K	Ubiquitination	E3 ubiquitin ligase	NEDD4	P46934	PMID:15620649

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of DTX1_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of DTX1_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
DTX3L_HUMAN	DTX3L	physical	12670957
DTX1_HUMAN	DTX1	physical	11564735
EP300_HUMAN	EP300	physical	11564735
GRB2_HUMAN	GRB2	physical	9590294
M3K1_HUMAN	MAP3K1	physical	15684388
NOTC1_HUMAN	NOTCH1	physical	20208568
EIF3F_HUMAN	EIF3F	physical	21124883
ITCH_HUMAN	ITCH	physical	17028573
G3BP1_HUMAN	G3BP1	physical	21988832

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.