CRY2_ARATH - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: CRY2_ARATH
• UniProt AC: Q96524
• Protein Name: Cryptochrome-2 {ECO:0000303|Ref.2}
• Gene Name: CRY2 {ECO:0000303|Ref.2}
• Organism: Arabidopsis thaliana (Mouse-ear cress).
• Sequence Length: 612
• Subcellular Localization: Nucleus . Nucleus, PML body . Cytoplasm . Present in nuclear bodies (NBs) in blue light (e.g. photobodies) (PubMed:21511872, PubMed:23833191, PubMed:22311776). Translocates from the cytosol to the nucleus in response to blue light illumination (PubMe
• Protein Description: Photoreceptor that mediates primarily blue light inhibition of hypocotyl elongation and photoperiodic control of floral initiation, and regulates other light responses, including circadian rhythms, tropic growth, stomata opening, guard cell development, root development, bacterial and viral pathogen responses, abiotic stress responses, cell cycles, programmed cell death, apical dominance, fruit and ovule development, seed dormancy, and magnetoreception. Photoexcited cryptochromes interact with signaling partner proteins to alter gene expression at both transcriptional and post-translational levels and, consequently, regulate the corresponding metabolic and developmental programs. [PubMed: 21841916 Blue-light absorbing flavoprotein that activates reversible flavin photoreduction via an electron transport chain comprising a tryptophan triad (W-321, W-374 and W-397), or via an alternative electron transport that involves small metabolites, including NADPH, NADH, and ATP. The half-life of the activated signaling state is about 16 minutes]
• Protein Sequence: MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQFYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLYDPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSIESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLNEFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGEESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPLVDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQYISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKASGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAAPDEIVADSFEALGANTIKEPGLCPSVSSNDQQVPSAVRYNGSKRVKPEEEEERDMKKSRGFDERELFSTAESSSSSSVFFVSQSCSLASEGKNLEGIQDSSDQITTSLGKNGCK

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
523	Phosphorylation	KEPGLCPSVSSNDQQ CCCCCCCCCCCCCCC	33.37	23776212
525	Phosphorylation	PGLCPSVSSNDQQVP CCCCCCCCCCCCCCC	27.97	23776212
526	Phosphorylation	GLCPSVSSNDQQVPS CCCCCCCCCCCCCCC	42.12	23776212
533	Phosphorylation	SNDQQVPSAVRYNGS CCCCCCCCCEECCCC	40.31	23776212
587	Phosphorylation	SQSCSLASEGKNLEG ECCCCCCCCCCCCCC	52.77	23897926
598	Phosphorylation	NLEGIQDSSDQITTS CCCCCCCCCHHHHHH	21.31	23776212
599	Phosphorylation	LEGIQDSSDQITTSL CCCCCCCCHHHHHHC	41.65	23776212
603	Phosphorylation	QDSSDQITTSLGKNG CCCCHHHHHHCCCCC	12.52	23776212
604	Phosphorylation	DSSDQITTSLGKNGC CCCHHHHHHCCCCCC	24.47	23776212
605	Phosphorylation	SSDQITTSLGKNGCK CCHHHHHHCCCCCCC	26.86	30291188

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
587	S	Phosphorylation	Kinase	CK1	Q9M9H6	Uniprot
603	T	Phosphorylation	Kinase	CK1	Q9M9H6	Uniprot

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of CRY2_ARATH !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of CRY2_ARATH !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
COP1_ARATH	COP1	genetic	15111714
CRY2_ARATH	CRY2	physical	17073458
BH063_ARATH	CIB1	physical	18988809
COP1_ARATH	COP1	physical	11509693
CRY2_ARATH	CRY2	physical	15805487
BH063_ARATH	CIB1	physical	21037589
SPA1_ARATH	SPA1	physical	21514160
COP1_ARATH	COP1	physical	21514160
SPA1_ARATH	SPA1	physical	22139370
BH063_ARATH	CIB1	physical	22139370
SPA1_ARATH	SPA1	physical	22739826
BH063_ARATH	CIB1	physical	22739826
RFWD2_MOUSE	Rfwd2	physical	23833191
BH076_ARATH	CIB5	physical	24130508
COP1_ARATH	COP1	physical	24554768
BH063_ARATH	CIB1	physical	22922268
BH063_ARATH	CIB1	physical	24780222
CRY2_ARATH	CRY2	physical	25233328
DET1_ARATH	DET1	genetic	25962685

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.