CPSF1_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: CPSF1_HUMAN
• UniProt AC: Q10570
• Protein Name: Cleavage and polyadenylation specificity factor subunit 1
• Gene Name: CPSF1
• Organism: Homo sapiens (Human).
• Sequence Length: 1443
• Subcellular Localization: Nucleus, nucleoplasm.
• Protein Description: Component of the cleavage and polyadenylation specificity factor (CPSF) complex that plays a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. This subunit is involved in the RNA recognition step of the polyadenylation reaction..
• Protein Sequence: MYAVYKQAHPPTGLEFSMYCNFFNNSERNLVVAGTSQLYVYRLNRDAEALTKNDRSTEGKAHREKLELAASFSFFGNVMSMASVQLAGAKRDALLLSFKDAKLSVVEYDPGTHDLKTLSLHYFEEPELRDGFVQNVHTPRVRVDPDGRCAAMLVYGTRLVVLPFRRESLAEEHEGLVGEGQRSSFLPSYIIDVRALDEKLLNIIDLQFLHGYYEPTLLILFEPNQTWPGRVAVRQDTCSIVAISLNITQKVHPVIWSLTSLPFDCTQALAVPKPIGGVVVFAVNSLLYLNQSVPPYGVALNSLTTGTTAFPLRTQEGVRITLDCAQATFISYDKMVISLKGGEIYVLTLITDGMRSVRAFHFDKAAASVLTTSMVTMEPGYLFLGSRLGNSLLLKYTEKLQEPPASAVREAADKEEPPSKKKRVDATAGWSAAGKSVPQDEVDEIEVYGSEAQSGTQLATYSFEVCDSILNIGPCANAAVGEPAFLSEEFQNSPEPDLEIVVCSGHGKNGALSVLQKSIRPQVVTTFELPGCYDMWTVIAPVRKEEEDNPKGEGTEQEPSTTPEADDDGRRHGFLILSREDSTMILQTGQEIMELDTSGFATQGPTVFAGNIGDNRYIVQVSPLGIRLLEGVNQLHFIPVDLGAPIVQCAVADPYVVIMSAEGHVTMFLLKSDSYGGRHHRLALHKPPLHHQSKVITLCLYRDLSGMFTTESRLGGARDELGGRSGPEAEGLGSETSPTVDDEEEMLYGDSGSLFSPSKEEARRSSQPPADRDPAPFRAEPTHWCLLVRENGTMEIYQLPDWRLVFLVKNFPVGQRVLVDSSFGQPTTQGEARREEATRQGELPLVKEVLLVALGSRQSRPYLLVHVDQELLIYEAFPHDSQLGQGNLKVRFKKVPHNINFREKKPKPSKKKAEGGGAEEGAGARGRVARFRYFEDIYGYSGVFICGPSPHWLLVTGRGALRLHPMAIDGPVDSFAPFHNVNCPRGFLYFNRQGELRISVLPAYLSYDAPWPVRKIPLRCTAHYVAYHVESKVYAVATSTNTPCARIPRMTGEEKEFETIERDERYIHPQQEAFSIQLISPVSWEAIPNARIELQEWEHVTCMKTVSLRSEETVSGLKGYVAAGTCLMQGEEVTCRGRILIMDVIEVVPEPGQPLTKNKFKVLYEKEQKGPVTALCHCNGHLVSAIGQKIFLWSLRASELTGMAFIDTQLYIHQMISVKNFILAADVMKSISLLRYQEESKTLSLVSRDAKPLEVYSVDFMVDNAQLGFLVSDRDRNLMVYMYLPEAKESFGGMRLLRRADFHVGAHVNTFWRTPCRGATEGLSKKSVVWENKHITWFATLDGGIGLLLPMQEKTYRRLLMLQNALTTMLPHHAGLNPRAFRMLHVDRRTLQNAVRNVLDGELLNRYLYLSTMERSELAKKIGTTPDIILDDLLETDRVTAHF

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Phosphorylation	------MYAVYKQAH ------CCEECCCCC	18.97	-
21	Ubiquitination	LEFSMYCNFFNNSER CEEEEEEECCCCCCC	27.36	23503661
24	Ubiquitination	SMYCNFFNNSERNLV EEEEECCCCCCCEEE	47.16	23503661
38	Ubiquitination	VVAGTSQLYVYRLNR EEEECCEEEEEECCC	2.90	21963094
52	Ubiquitination	RDAEALTKNDRSTEG CCHHHHHCCCCCCCC	58.67	21906983
52	Acetylation	RDAEALTKNDRSTEG CCHHHHHCCCCCCCC	58.67	25953088
71	Phosphorylation	EKLELAASFSFFGNV HHHHHHHHCCHHHHH	18.80	26074081
73	Phosphorylation	LELAASFSFFGNVMS HHHHHHCCHHHHHHH	19.71	26074081
80	Phosphorylation	SFFGNVMSMASVQLA CHHHHHHHHHHHHHH	13.60	26074081
83	Phosphorylation	GNVMSMASVQLAGAK HHHHHHHHHHHHCCC	11.18	26074081
97	Phosphorylation	KRDALLLSFKDAKLS CCCEEEEEECCCEEE	30.16	24719451
99	Ubiquitination	DALLLSFKDAKLSVV CEEEEEECCCEEEEE	54.09	23503661
101	Ubiquitination	LLLSFKDAKLSVVEY EEEEECCCEEEEEEE	18.45	22817900
102	Ubiquitination	LLSFKDAKLSVVEYD EEEECCCEEEEEEEC	51.97	23503661
116	Ubiquitination	DPGTHDLKTLSLHYF CCCCCCCCEEEEEEE	54.18	21906983
117	Phosphorylation	PGTHDLKTLSLHYFE CCCCCCCEEEEEEEC	28.95	25841592
122	Phosphorylation	LKTLSLHYFEEPELR CCEEEEEEECCHHHC	20.27	25841592
138	Phosphorylation	GFVQNVHTPRVRVDP CCCCCCCCCCEEECC	14.43	28985074
151	Ubiquitination	DPDGRCAAMLVYGTR CCCCCEEEEEEECCE	8.75	21890473
168	Phosphorylation	VLPFRRESLAEEHEG EEECCHHHHHHHCCC	30.94	28985074
249	Ubiquitination	AISLNITQKVHPVIW EEEECCCCCCCCCHH	41.81	24816145
317	Ubiquitination	FPLRTQEGVRITLDC CCCCCCCCEEEEEEC	12.27	21963094
321	Phosphorylation	TQEGVRITLDCAQAT CCCCEEEEEECEECE	13.14	20068231
321	Ubiquitination	TQEGVRITLDCAQAT CCCCEEEEEECEECE	13.14	22817900
328	Phosphorylation	TLDCAQATFISYDKM EEECEECEECEEEEE	14.93	20068231
331	Phosphorylation	CAQATFISYDKMVIS CEECEECEEEEEEEE	24.08	20068231
332	Phosphorylation	AQATFISYDKMVISL EECEECEEEEEEEEE	18.11	20068231
336	Ubiquitination	FISYDKMVISLKGGE ECEEEEEEEEECCCE	3.11	24816145
338	Phosphorylation	SYDKMVISLKGGEIY EEEEEEEEECCCEEE	16.78	24719451
344	Ubiquitination	ISLKGGEIYVLTLIT EEECCCEEEEEEEEC	3.05	24816145
345	Phosphorylation	SLKGGEIYVLTLITD EECCCEEEEEEEECC	5.77	20068231
351	Phosphorylation	IYVLTLITDGMRSVR EEEEEEECCCCCCEE	29.61	20068231
364	Ubiquitination	VRAFHFDKAAASVLT EEECCCCHHHHHHHH	38.69	-
395	Ubiquitination	LGNSLLLKYTEKLQE HCCHHHHHHHHHHCC	50.53	21906983
395	Acetylation	LGNSLLLKYTEKLQE HCCHHHHHHHHHHCC	50.53	26051181
397	Ubiquitination	NSLLLKYTEKLQEPP CHHHHHHHHHHCCCC	25.67	22817900
399	Ubiquitination	LLLKYTEKLQEPPAS HHHHHHHHHCCCCHH	47.90	21906983
399	Acetylation	LLLKYTEKLQEPPAS HHHHHHHHHCCCCHH	47.90	26051181
406	Phosphorylation	KLQEPPASAVREAAD HHCCCCHHHHHHHHC	32.82	28555341
414	Ubiquitination	AVREAADKEEPPSKK HHHHHHCCCCCCCCC	60.29	24816145
414	Acetylation	AVREAADKEEPPSKK HHHHHHCCCCCCCCC	60.29	26051181
422	Acetylation	EEPPSKKKRVDATAG CCCCCCCCCCCCCCC	62.48	18529077
422	Ubiquitination	EEPPSKKKRVDATAG CCCCCCCCCCCCCCC	62.48	24816145
431	Phosphorylation	VDATAGWSAAGKSVP CCCCCCCCCCCCCCC	13.81	28555341
435	Acetylation	AGWSAAGKSVPQDEV CCCCCCCCCCCHHHC	43.48	18529085
446	Ubiquitination	QDEVDEIEVYGSEAQ HHHCCEEEEECCCCC	27.86	21963094
460	Ubiquitination	QSGTQLATYSFEVCD CCCCEEEEEEHHHCC	28.43	21963094
473	Ubiquitination	CDSILNIGPCANAAV CCHHHCCCCCCCCCC	15.34	22817900
499	Ubiquitination	NSPEPDLEIVVCSGH CCCCCCCEEEEEECC	40.91	22817900
501	Ubiquitination	PEPDLEIVVCSGHGK CCCCCEEEEEECCCC	2.31	22817900
503	Ubiquitination	PDLEIVVCSGHGKNG CCCEEEEEECCCCCC	2.53	21890473
508	Ubiquitination	VVCSGHGKNGALSVL EEEECCCCCCHHHHH	46.67	22817900
511	Ubiquitination	SGHGKNGALSVLQKS ECCCCCCHHHHHHHC	13.17	22817900
551	Ubiquitination	KEEEDNPKGEGTEQE CCCCCCCCCCCCCCC	75.69	21906983
560	Phosphorylation	EGTEQEPSTTPEADD CCCCCCCCCCCCCCC	44.13	-
562	Phosphorylation	TEQEPSTTPEADDDG CCCCCCCCCCCCCCC	24.38	28985074
583	Ubiquitination	ILSREDSTMILQTGQ EEECCCCEEEEECCC	22.13	21890473
617	Phosphorylation	GNIGDNRYIVQVSPL CEECCCEEEEEECHH	16.23	28152594
674	Phosphorylation	MFLLKSDSYGGRHHR EEEEECCCCCCCCCE	32.54	22798277
675	Phosphorylation	FLLKSDSYGGRHHRL EEEECCCCCCCCCEE	28.70	22798277
681	Ubiquitination	SYGGRHHRLALHKPP CCCCCCCEEEECCCC	18.61	22817900
686	Acetylation	HHRLALHKPPLHHQS CCEEEECCCCCCCCC	49.53	7704233
686	Ubiquitination	HHRLALHKPPLHHQS CCEEEECCCCCCCCC	49.53	29967540
694	Acetylation	PPLHHQSKVITLCLY CCCCCCCCHHHHHHE	30.96	7704243
705	Phosphorylation	LCLYRDLSGMFTTES HHHEECCCCCCCCCH	32.28	20068231
709	Phosphorylation	RDLSGMFTTESRLGG ECCCCCCCCCHHHCC	22.29	20068231
710	Phosphorylation	DLSGMFTTESRLGGA CCCCCCCCCHHHCCC	22.24	20068231
712	Phosphorylation	SGMFTTESRLGGARD CCCCCCCHHHCCCCC	30.61	20068231
725	Phosphorylation	RDELGGRSGPEAEGL CCCCCCCCCCCCCCC	63.07	28464451
731	Ubiquitination	RSGPEAEGLGSETSP CCCCCCCCCCCCCCC	42.24	21890473
734	Phosphorylation	PEAEGLGSETSPTVD CCCCCCCCCCCCCCC	42.62	30108239
736	Phosphorylation	AEGLGSETSPTVDDE CCCCCCCCCCCCCCH	41.52	28176443
737	Phosphorylation	EGLGSETSPTVDDEE CCCCCCCCCCCCCHH	17.96	28176443
739	Phosphorylation	LGSETSPTVDDEEEM CCCCCCCCCCCHHHH	36.01	28176443
748	Phosphorylation	DDEEEMLYGDSGSLF CCHHHHHHCCCCCCC	19.99	28176443
751	Phosphorylation	EEMLYGDSGSLFSPS HHHHHCCCCCCCCCC	26.39	23401153
753	Phosphorylation	MLYGDSGSLFSPSKE HHHCCCCCCCCCCHH	30.14	28176443
756	Phosphorylation	GDSGSLFSPSKEEAR CCCCCCCCCCHHHHH	33.29	23401153
758	Phosphorylation	SGSLFSPSKEEARRS CCCCCCCCHHHHHHH	52.05	28176443
759	Ubiquitination	GSLFSPSKEEARRSS CCCCCCCHHHHHHHC	64.06	21906983
762	Ubiquitination	FSPSKEEARRSSQPP CCCCHHHHHHHCCCC	17.18	22817900
763	Ubiquitination	SPSKEEARRSSQPPA CCCHHHHHHHCCCCC	42.15	22817900
765	Phosphorylation	SKEEARRSSQPPADR CHHHHHHHCCCCCCC	27.98	30266825
766	Phosphorylation	KEEARRSSQPPADRD HHHHHHHCCCCCCCC	44.91	23401153
809	Acetylation	WRLVFLVKNFPVGQR EEEEEEECCCCCCCE	55.83	26051181
809	Ubiquitination	WRLVFLVKNFPVGQR EEEEEEECCCCCCCE	55.83	23000965
829	Ubiquitination	SFGQPTTQGEARREE CCCCCCCCHHHHHHH	50.65	24816145
847	Ubiquitination	QGELPLVKEVLLVAL CCCCCHHHHHHHHHH	50.19	33845483
859	Phosphorylation	VALGSRQSRPYLLVH HHHCCCCCCCEEEEE	33.83	-
893	Ubiquitination	GNLKVRFKKVPHNIN CCEEEEEEECCCCCC	42.15	-
894	Ubiquitination	NLKVRFKKVPHNINF CEEEEEEECCCCCCC	59.28	29967540
907	Ubiquitination	NFREKKPKPSKKKAE CCCCCCCCCCCCCCC	71.45	24816145
933	Phosphorylation	GRVARFRYFEDIYGY CCEEEEEECCHHHCC	14.49	22210691
941	Phosphorylation	FEDIYGYSGVFICGP CCHHHCCCEEEEECC	24.90	22210691
949	Phosphorylation	GVFICGPSPHWLLVT EEEEECCCCCEEEEE	18.06	-
956	Phosphorylation	SPHWLLVTGRGALRL CCCEEEEECCCCEEE	22.56	24719451
977	Ubiquitination	GPVDSFAPFHNVNCP CCCCCCCCCCCCCCC	29.08	22817900
1026	Ubiquitination	RCTAHYVAYHVESKV CCEEEEEEEEEECCE	4.99	21963094
1034	Phosphorylation	YHVESKVYAVATSTN EEEECCEEEEEECCC	10.09	28152594
1040	Ubiquitination	VYAVATSTNTPCARI EEEEEECCCCCCCCC	38.46	21963094
1042	Phosphorylation	AVATSTNTPCARIPR EEEECCCCCCCCCCC	21.60	25159151
1051	Phosphorylation	CARIPRMTGEEKEFE CCCCCCCCCCCEEEE	42.60	30576142
1055	Ubiquitination	PRMTGEEKEFETIER CCCCCCCEEEEEEEC	64.50	21906983
1079	Ubiquitination	EAFSIQLISPVSWEA CEEEEEEECCCCHHH	2.21	22817900
1081	Ubiquitination	FSIQLISPVSWEAIP EEEEEECCCCHHHCC	19.71	22817900
1083	Ubiquitination	IQLISPVSWEAIPNA EEEECCCCHHHCCCC	23.68	21890473
1088	Ubiquitination	PVSWEAIPNARIELQ CCCHHHCCCCEEEEE	35.16	22817900
1091	Ubiquitination	WEAIPNARIELQEWE HHHCCCCEEEEEECE	29.56	22817900
1104	Ubiquitination	WEHVTCMKTVSLRSE CEEEEEEEEEECCCC	48.17	21963094
1118	Ubiquitination	EETVSGLKGYVAAGT CCHHCCCCCEEEEEE	52.28	21906983
1156	Phosphorylation	PEPGQPLTKNKFKVL CCCCCCCCCCCEEEE	38.97	29900121
1157	Ubiquitination	EPGQPLTKNKFKVLY CCCCCCCCCCEEEEE	66.86	21906983
1159	Ubiquitination	GQPLTKNKFKVLYEK CCCCCCCCEEEEEEH	48.39	22817900
1161	Ubiquitination	PLTKNKFKVLYEKEQ CCCCCCEEEEEEHHC	32.70	22817900
1163	Ubiquitination	TKNKFKVLYEKEQKG CCCCEEEEEEHHCCC	4.75	21890473
1166	Ubiquitination	KFKVLYEKEQKGPVT CEEEEEEHHCCCCEE	52.46	21906983
1169	Ubiquitination	VLYEKEQKGPVTALC EEEEHHCCCCEEEEE	67.29	22817900
1194	Phosphorylation	GQKIFLWSLRASELT HHHHHHHHHCHHHHH	15.32	20068231
1217	Phosphorylation	LYIHQMISVKNFILA HHHHHHHCCCHHHHH	23.00	24719451
1241	Ubiquitination	LRYQEESKTLSLVSR HHCHHHCCCEEEECC	57.68	27667366
1281	Phosphorylation	RDRNLMVYMYLPEAK CCCCEEEEEECHHHH	2.62	25072903
1283	Phosphorylation	RNLMVYMYLPEAKES CCEEEEEECHHHHHH	11.04	25072903
1288	Ubiquitination	YMYLPEAKESFGGMR EEECHHHHHHCCCCC	52.83	-
1325	Acetylation	GATEGLSKKSVVWEN CCCCCCCCCEEEEEC	54.86	25953088
1326	Ubiquitination	ATEGLSKKSVVWENK CCCCCCCCEEEEECC	45.14	29967540
1342	Ubiquitination	ITWFATLDGGIGLLL EEEEEEECCCCEEEE	49.25	22817900
1343	Ubiquitination	TWFATLDGGIGLLLP EEEEEECCCCEEEEE	32.50	22817900
1417	Ubiquitination	LSTMERSELAKKIGT HHHHCHHHHHHHHCC	59.99	22817900
1418	Ubiquitination	STMERSELAKKIGTT HHHCHHHHHHHHCCC	9.99	22817900
1420	Ubiquitination	MERSELAKKIGTTPD HCHHHHHHHHCCCCC	58.51	22817900
1421	Ubiquitination	ERSELAKKIGTTPDI CHHHHHHHHCCCCCC	40.54	21906983

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of CPSF1_HUMAN !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of CPSF1_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of CPSF1_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
CSN5_HUMAN	COPS5	physical	18173839
CSN2_HUMAN	COPS2	physical	18173839
CSTF3_HUMAN	CSTF3	physical	7590244
PAPOA_HUMAN	PAPOLA	physical	7590244
CSTFT_HUMAN	CSTF2T	physical	7590244
CPSF2_HUMAN	CPSF2	physical	22939629
CPSF3_HUMAN	CPSF3	physical	22939629
FIP1_HUMAN	FIP1L1	physical	22939629
WDR33_HUMAN	WDR33	physical	22939629
RBM40_HUMAN	RNPC3	physical	22939629
TF3C3_HUMAN	GTF3C3	physical	22939629
MED8_HUMAN	MED8	physical	22939629
NPM_HUMAN	NPM1	physical	21822216
HMOX1_HUMAN	HMOX1	physical	21102410
IL7RA_HUMAN	IL7R	physical	23151878
CPSF2_HUMAN	CPSF2	physical	26344197
CPSF3_HUMAN	CPSF3	physical	26344197
CPSF4_HUMAN	CPSF4	physical	26344197
CSTF2_HUMAN	CSTF2	physical	26344197
PP1B_HUMAN	PPP1CB	physical	26344197
RAGP1_HUMAN	RANGAP1	physical	26344197
SYMPK_HUMAN	SYMPK	physical	26344197
WDR33_HUMAN	WDR33	physical	26344197
CSN1_HUMAN	GPS1	physical	18173839

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• There are no disease associations of PTM sites.
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"Large-scale characterization of HeLa cell nuclear phosphoproteins.";
Beausoleil S.A., Jedrychowski M., Schwartz D., Elias J.E., Villen J.,Li J., Cohn M.A., Cantley L.C., Gygi S.P.;
Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-739, AND MASSSPECTROMETRY.
PubMed: 15302935