AP2B_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: AP2B_HUMAN
• UniProt AC: Q92481
• Protein Name: Transcription factor AP-2-beta
• Gene Name: TFAP2B
• Organism: Homo sapiens (Human).
• Sequence Length: 460
• Subcellular Localization: Nucleus .
• Protein Description: Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. AP-2-beta appears to be required for normal face and limb development and for proper terminal differentiation and function of renal tubular epithelia..
• Protein Sequence: MHSPPRDQAAIMLWKLVENVKYEDIYEDRHDGVPSHSSRLSQLGSVSQGPYSSAPPLSHTPSSDFQPPYFPPPYQPLPYHQSQDPYSHVNDPYSLNPLHQPQQHPWGQRQRQEVGSEAGSLLPQPRAALPQLSGLDPRRDYHSVRRPDVLLHSAHHGLDAGMGDSLSLHGLGHPGMEDVQSVEDANNSGMNLLDQSVIKKVPVPPKSVTSLMMNKDGFLGGMSVNTGEVFCSVPGRLSLLSSTSKYKVTVGEVQRRLSPPECLNASLLGGVLRRAKSKNGGRSLRERLEKIGLNLPAGRRKAANVTLLTSLVEGEAVHLARDFGYICETEFPAKAVSEYLNRQHTDPSDLHSRKNMLLATKQLCKEFTDLLAQDRTPIGNSRPSPILEPGIQSCLTHFSLITHGFGAPAICAALTALQNYLTEALKGMDKMFLNNTTTNRHTSGEGPGSKTGDKEEKHRK

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
3	Phosphorylation	-----MHSPPRDQAA -----CCCCHHHHHH	34.99	101546443
15	Ubiquitination	QAAIMLWKLVENVKY HHHHHHHHHHHHCCH	38.40	-
21	Sumoylation	WKLVENVKYEDIYED HHHHHHCCHHHHCCC	55.28	12072434
21	Sumoylation	WKLVENVKYEDIYED HHHHHHCCHHHHCCC	55.28	-
26	Phosphorylation	NVKYEDIYEDRHDGV HCCHHHHCCCCCCCC	24.89	22817900
92	Ubiquitination	PYSHVNDPYSLNPLH CCCCCCCCCCCCCCC	18.53	21963094
94	Ubiquitination	SHVNDPYSLNPLHQP CCCCCCCCCCCCCCC	27.36	22817900
120	Phosphorylation	EVGSEAGSLLPQPRA HHHHHHHHCCCCHHH	33.51	50565749
223	Phosphorylation	DGFLGGMSVNTGEVF CCCCCCEEECCCCEE	18.58	20068231
226	Phosphorylation	LGGMSVNTGEVFCSV CCCEEECCCCEEEEC	32.34	20068231
232	Phosphorylation	NTGEVFCSVPGRLSL CCCCEEEECCCCHHH	21.63	20068231
238	Phosphorylation	CSVPGRLSLLSSTSK EECCCCHHHCCCCCC	26.38	51163003
241	Phosphorylation	PGRLSLLSSTSKYKV CCCHHHCCCCCCEEE	36.54	22617229
242	Phosphorylation	GRLSLLSSTSKYKVT CCHHHCCCCCCEEEE	36.35	29255136
243	Phosphorylation	RLSLLSSTSKYKVTV CHHHCCCCCCEEEEH	26.34	23663014
244	Phosphorylation	LSLLSSTSKYKVTVG HHHCCCCCCEEEEHH	35.62	30576142
245	Ubiquitination	SLLSSTSKYKVTVGE HHCCCCCCEEEEHHH	49.53	21963094
246	Phosphorylation	LLSSTSKYKVTVGEV HCCCCCCEEEEHHHH	15.51	20068231
247	Ubiquitination	LSSTSKYKVTVGEVQ CCCCCCEEEEHHHHH	34.86	22817900
254	Ubiquitination	KVTVGEVQRRLSPPE EEEHHHHHHHCCCHH	22.07	21963094
256	Ubiquitination	TVGEVQRRLSPPECL EHHHHHHHCCCHHHH	23.56	22817900
258	Phosphorylation	GEVQRRLSPPECLNA HHHHHHCCCHHHHCH	36.28	24732914
266	Phosphorylation	PPECLNASLLGGVLR CHHHHCHHHHHHHHH	24.05	24732914
277	Phosphorylation	GVLRRAKSKNGGRSL HHHHHHHHCCCCHHH	30.30	-
288	Ubiquitination	GRSLRERLEKIGLNL CHHHHHHHHHHCCCC	7.24	21963094
290	Ubiquitination	SLRERLEKIGLNLPA HHHHHHHHHCCCCCC	46.82	22817900
300	Ubiquitination	LNLPAGRRKAANVTL CCCCCCCHHHCCCEE	31.86	21963094
301	Ubiquitination	NLPAGRRKAANVTLL CCCCCCHHHCCCEEE	50.95	-
302	Ubiquitination	LPAGRRKAANVTLLT CCCCCHHHCCCEEEE	11.49	22817900
354	Ubiquitination	PSDLHSRKNMLLATK HHHHHHHHHHHHHHH	50.12	-
442	Phosphorylation	NTTTNRHTSGEGPGS CCCCCCCCCCCCCCC	34.88	27732954
443	Phosphorylation	TTTNRHTSGEGPGSK CCCCCCCCCCCCCCC	28.81	27732954

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
258	S	Phosphorylation	Kinase	PKA	-	Uniprot

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
21	K	Sumoylation	Sumoylated on Lys-21; which inhibits transcriptional activity.	12072434

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of AP2B_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
LZTR1_HUMAN	LZTR1	physical	20211142
SSBP4_HUMAN	SSBP4	physical	20211142
UBC9_HUMAN	UBE2I	physical	12072434
YETS4_HUMAN	YEATS4	physical	16698963
CBP_HUMAN	CREBBP	physical	25294805

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• 169100: Char syndrome (CHAR)
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.