dbPTM

AGAP2_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	AGAP2_HUMAN
UniProt AC	Q99490
Protein Name	Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2
Gene Name	AGAP2
Organism	Homo sapiens (Human).
Sequence Length	1192
Subcellular Localization	Isoform 1: Cytoplasm. Nucleus. Isoform 2: Cytoplasm.
Protein Description	GTPase-activating protein (GAP) for ARF1 and ARF5, which also shows strong GTPase activity. Isoform 1 participates in the prevention of neuronal apoptosis by enhancing PI3 kinase activity. It aids the coupling of metabotropic glutamate receptor 1 (GRM1) to cytoplasmic PI3 kinase by interacting with Homer scaffolding proteins, and also seems to mediate anti-apoptotic effects of NGF by activating nuclear PI3 kinase. Isoform 2 does not stimulate PI3 kinase but may protect cells from apoptosis by stimulating Akt. It also regulates the adapter protein 1 (AP-1)-dependent trafficking of proteins in the endosomal system. It seems to be oncogenic. It is overexpressed in cancer cells, prevents apoptosis and promotes cancer cell invasion..
Protein Sequence	MSRGAGALQRRTTTYLISLTLVKLESVPPPPPSPSAAAVGAPGARGSEPRDPGSPRGAEEPGKKRHERLFHRQDALWISTSSAGAGGAEPPALSPAPASPARPVSPAPGRRLSLWAAPPGPPLSGGLSPDSKPGGAPSSSRRPLLSSPSWGGPEPEGRTGGGVPGSSSPHPGTGSRRLKVAPPPPAPKPCKTVTTSGAKAGGGKGAGSRLSWPESEGKPRVKGSKSSAGTGASVSAAATAAAAGGGGSTASTSGGVGAGAGARGKLSPRKGKSKTLDNSDLHPGPPAGSPPPLTLPPTPSPATAVTAASAQPPGPAPPITLEPPAPGLKRGREGGRASTRDRKMLKFISGIFTKSTGGPPGSGPLPGPPSLSSGSGSRELLGAELRASPKAVINSQEWTLSRSIPELRLGVLGDARSGKSSLIHRFLTGSYQVLEKTESEQYKKEMLVDGQTHLVLIREEAGAPDAKFSGWADAVIFVFSLEDENSFQAVSRLHGQLSSLRGEGRGGLALALVGTQDRISASSPRVVGDARARALCADMKRCSYYETCATYGLNVDRVFQEVAQKVVTLRKQQQLLAACKSLPSSPSHSAASTPVAGQASNGGHTSDYSSSLPSSPNVGHRELRAEAAAVAGLSTPGSLHRAAKRRTSLFANRRGSDSEKRSLDSRGETTGSGRAIPIKQSFLLKRSGNSLNKEWKKKYVTLSSNGFLLYHPSINDYIHSTHGKEMDLLRTTVKVPGKRPPRAISAFGPSASINGLVKDMSTVQMGEGLEATTPMPSPSPSPSSLQPPPDQTSKHLLKPDRNLARALSTDCTPSGDLSPLSREPPPSPMVKKQRRKKLTTPSKTEGSAGQAEAKRKMWKLKSFGSLRNIYKAEENFEFLIVSSTGQTWHFEAASFEERDAWVQAIESQILASLQCCESSKVKLRTDSQSEAVAIQAIRNAKGNSICVDCGAPNPTWASLNLGALICIECSGIHRNLGTHLSRVRSLDLDDWPRELTLVLTAIGNDTANRVWESDTRGRAKPSRDSSREERESWIRAKYEQLLFLAPLSTSEEPLGRQLWAAVQAQDVATVLLLLAHARHGPLDTSVEDPQLRSPLHLAAELAHVVITQLLLWYGADVAARDAQGRTALFYARQAGSQLCADILLQHGCPGEGGSAATTPSAATTPSITATPSPRRRSSAASVGRADAPVALV

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Phosphorylation	------MSRGAGALQ ------CCCCCHHCH	51.21	-
3	Methylation	-----MSRGAGALQR -----CCCCCHHCHH	51.33	-
35	Phosphorylation	PPPPPSPSAAAVGAP CCCCCCCCHHCCCCC	34.57	19690332
113	Phosphorylation	PAPGRRLSLWAAPPG CCCCCEEEEEECCCC	21.76	-
128	Phosphorylation	PPLSGGLSPDSKPGG CCCCCCCCCCCCCCC	29.90	-
138	Phosphorylation	SKPGGAPSSSRRPLL CCCCCCCCCCCCCCC	40.17	28787133
140	Phosphorylation	PGGAPSSSRRPLLSS CCCCCCCCCCCCCCC	36.32	22210691
146	Phosphorylation	SSRRPLLSSPSWGGP CCCCCCCCCCCCCCC	48.13	24732914
147	Phosphorylation	SRRPLLSSPSWGGPE CCCCCCCCCCCCCCC	23.95	24732914
149	Phosphorylation	RPLLSSPSWGGPEPE CCCCCCCCCCCCCCC	40.06	26437602
208	Phosphorylation	GGGKGAGSRLSWPES CCCCCCCCCCCCCHH	29.52	25332170
211	Phosphorylation	KGAGSRLSWPESEGK CCCCCCCCCCHHCCC	39.20	25307156
224	Phosphorylation	GKPRVKGSKSSAGTG CCCCCCCCCCCCCCC	24.52	28857561
226	Phosphorylation	PRVKGSKSSAGTGAS CCCCCCCCCCCCCHH	27.41	28857561
227	Phosphorylation	RVKGSKSSAGTGASV CCCCCCCCCCCCHHH	34.16	22210691
233	Phosphorylation	SSAGTGASVSAAATA CCCCCCHHHHHHHHH	20.25	22210691
235	Phosphorylation	AGTGASVSAAATAAA CCCCHHHHHHHHHHH	15.08	28787133
248	Phosphorylation	AAAGGGGSTASTSGG HHCCCCCCCCCCCCC	24.87	22817900
249	Phosphorylation	AAGGGGSTASTSGGV HCCCCCCCCCCCCCC	28.09	23403867
251	Phosphorylation	GGGGSTASTSGGVGA CCCCCCCCCCCCCCC	24.16	22210691
252	Phosphorylation	GGGSTASTSGGVGAG CCCCCCCCCCCCCCC	29.00	22817900
253	Phosphorylation	GGSTASTSGGVGAGA CCCCCCCCCCCCCCC	30.93	23403867
267	Phosphorylation	AGARGKLSPRKGKSK CCCCCCCCCCCCCCC	26.58	23532336
403	Phosphorylation	QEWTLSRSIPELRLG HHHHHCCCCCCCEEC	38.84	24719451
431	Phosphorylation	HRFLTGSYQVLEKTE HHHHHCCHHHHHHCC	12.18	24076635
498	Phosphorylation	SRLHGQLSSLRGEGR HHHHHHHHHHCCCCC	21.31	30301811
499	Phosphorylation	RLHGQLSSLRGEGRG HHHHHHHHHCCCCCC	30.58	25954137
505	Methylation	SSLRGEGRGGLALAL HHHCCCCCCHHEEEE	31.53	-
520	Phosphorylation	VGTQDRISASSPRVV EECCCCCCCCCCCHH	24.00	26699800
522	Phosphorylation	TQDRISASSPRVVGD CCCCCCCCCCCHHCH	33.80	24719451
523	Phosphorylation	QDRISASSPRVVGDA CCCCCCCCCCHHCHH	19.22	30576142
540	Ubiquitination	RALCADMKRCSYYET HHHHCHHCCCCHHHH	51.29	-
544	Phosphorylation	ADMKRCSYYETCATY CHHCCCCHHHHHHHH	14.67	-
545	Phosphorylation	DMKRCSYYETCATYG HHCCCCHHHHHHHHC	6.62	-
565	Ubiquitination	VFQEVAQKVVTLRKQ HHHHHHHHHHHHHHH	29.00	-
571	Ubiquitination	QKVVTLRKQQQLLAA HHHHHHHHHHHHHHH	56.42	-
581	Phosphorylation	QLLAACKSLPSSPSH HHHHHHHCCCCCCCC	45.45	28450419
584	Phosphorylation	AACKSLPSSPSHSAA HHHHCCCCCCCCCCC	61.24	28450419
585	Phosphorylation	ACKSLPSSPSHSAAS HHHCCCCCCCCCCCC	28.60	28450419
587	Phosphorylation	KSLPSSPSHSAASTP HCCCCCCCCCCCCCC	31.83	28450419
589	Phosphorylation	LPSSPSHSAASTPVA CCCCCCCCCCCCCCC	29.84	28464451
592	Phosphorylation	SPSHSAASTPVAGQA CCCCCCCCCCCCCCC	32.72	27080861
593	Phosphorylation	PSHSAASTPVAGQAS CCCCCCCCCCCCCCC	19.63	28450419
600	Phosphorylation	TPVAGQASNGGHTSD CCCCCCCCCCCCCCC	28.28	23401153
605	Phosphorylation	QASNGGHTSDYSSSL CCCCCCCCCCCCCCC	26.94	28450419
606	Phosphorylation	ASNGGHTSDYSSSLP CCCCCCCCCCCCCCC	28.95	28450419
608	Phosphorylation	NGGHTSDYSSSLPSS CCCCCCCCCCCCCCC	15.37	28450419
609	Phosphorylation	GGHTSDYSSSLPSSP CCCCCCCCCCCCCCC	20.59	23401153
610	Phosphorylation	GHTSDYSSSLPSSPN CCCCCCCCCCCCCCC	29.66	28450419
611	Phosphorylation	HTSDYSSSLPSSPNV CCCCCCCCCCCCCCC	38.06	28464451
614	Phosphorylation	DYSSSLPSSPNVGHR CCCCCCCCCCCCCCH	64.82	23401153
615	Phosphorylation	YSSSLPSSPNVGHRE CCCCCCCCCCCCCHH	20.67	28450419
634	Phosphorylation	AAAVAGLSTPGSLHR HHHHHCCCCHHHHHH	31.47	28450419
635	Phosphorylation	AAVAGLSTPGSLHRA HHHHCCCCHHHHHHH	36.39	23401153
638	Phosphorylation	AGLSTPGSLHRAAKR HCCCCHHHHHHHHHH	23.39	23401153
647	Phosphorylation	HRAAKRRTSLFANRR HHHHHHHHHHHCCCC	33.54	30266825
648	Phosphorylation	RAAKRRTSLFANRRG HHHHHHHHHHCCCCC	21.48	23401153
656	Phosphorylation	LFANRRGSDSEKRSL HHCCCCCCHHHCCCC	35.83	22817900
679	Ubiquitination	SGRAIPIKQSFLLKR CCCEEECCHHEEECC	34.24	-
681	Phosphorylation	RAIPIKQSFLLKRSG CEEECCHHEEECCCC	16.92	28122231
682 (in isoform 2)	Phosphorylation	-	9.55	16841086
685	Methylation	IKQSFLLKRSGNSLN CCHHEEECCCCCCCC	46.82	-
690	Phosphorylation	LLKRSGNSLNKEWKK EECCCCCCCCHHHHH	36.52	29978859
734	Ubiquitination	DLLRTTVKVPGKRPP EEEEEEEECCCCCCC	40.39	-
745	Phosphorylation	KRPPRAISAFGPSAS CCCCCCHHHCCCCCH	19.29	26074081
750	Phosphorylation	AISAFGPSASINGLV CHHHCCCCCHHCCHH	34.72	26074081
752	Phosphorylation	SAFGPSASINGLVKD HHCCCCCHHCCHHCC	22.41	26074081
774 (in isoform 2)	Phosphorylation	-	28.47	16841086
808	Phosphorylation	RNLARALSTDCTPSG HHHHHHHCCCCCCCC	22.63	23401153
809	Phosphorylation	NLARALSTDCTPSGD HHHHHHCCCCCCCCC	35.62	30266825
812	Phosphorylation	RALSTDCTPSGDLSP HHHCCCCCCCCCCCC	24.25	30266825
814	Phosphorylation	LSTDCTPSGDLSPLS HCCCCCCCCCCCCCC	27.13	30266825
818	Phosphorylation	CTPSGDLSPLSREPP CCCCCCCCCCCCCCC	28.65	28122231
821	Phosphorylation	SGDLSPLSREPPPSP CCCCCCCCCCCCCCC	37.67	28122231
827	O-linked_Glycosylation	LSREPPPSPMVKKQR CCCCCCCCCCCCHHH	31.25	30379171
827	Phosphorylation	LSREPPPSPMVKKQR CCCCCCCCCCCCHHH	31.25	23401153
831	Acetylation	PPPSPMVKKQRRKKL CCCCCCCCHHHHHCC	36.62	7704497
832	Acetylation	PPSPMVKKQRRKKLT CCCCCCCHHHHHCCC	37.31	6570039
839	Phosphorylation	KQRRKKLTTPSKTEG HHHHHCCCCCCCCCC	45.61	28060719
894	Phosphorylation	TWHFEAASFEERDAW EEEEEECCHHHHHHH	39.99	24719451
925	Phosphorylation	SSKVKLRTDSQSEAV HCCEEEECCCHHHHH	50.90	30266825
927	Phosphorylation	KVKLRTDSQSEAVAI CEEEECCCHHHHHHH	34.69	23401153
929	Phosphorylation	KLRTDSQSEAVAIQA EEECCCHHHHHHHHH	31.32	30266825
985	Phosphorylation	THLSRVRSLDLDDWP HHHHHCCCCCCCCCC	24.31	24247654
1006	Phosphorylation	LTAIGNDTANRVWES EEEECCCHHHCCCCC	29.81	-
1037	Ubiquitination	RESWIRAKYEQLLFL HHHHHHHHHHHHHHH	39.20	-
1038	Phosphorylation	ESWIRAKYEQLLFLA HHHHHHHHHHHHHHE	14.13	16841086
1130	Phosphorylation	QGRTALFYARQAGSQ CCCHHHHHHHHHHCH	11.17	16841086
1154	Phosphorylation	GCPGEGGSAATTPSA CCCCCCCCCCCCCCC	26.11	26074081
1157	Phosphorylation	GEGGSAATTPSAATT CCCCCCCCCCCCCCC	38.67	26074081
1158	Phosphorylation	EGGSAATTPSAATTP CCCCCCCCCCCCCCC	15.54	26074081
1160	Phosphorylation	GSAATTPSAATTPSI CCCCCCCCCCCCCCC	28.57	26074081
1163	Phosphorylation	ATTPSAATTPSITAT CCCCCCCCCCCCCCC	38.67	26074081
1164	Phosphorylation	TTPSAATTPSITATP CCCCCCCCCCCCCCC	15.43	26074081
1166	Phosphorylation	PSAATTPSITATPSP CCCCCCCCCCCCCCC	30.40	26074081
1168	Phosphorylation	AATTPSITATPSPRR CCCCCCCCCCCCCCC	28.90	23401153
1170	Phosphorylation	TTPSITATPSPRRRS CCCCCCCCCCCCCCC	18.69	26074081
1172	Phosphorylation	PSITATPSPRRRSSA CCCCCCCCCCCCCCC	27.04	26074081
1177	Phosphorylation	TPSPRRRSSAASVGR CCCCCCCCCCCCCCC	23.63	30576142
1178	Phosphorylation	PSPRRRSSAASVGRA CCCCCCCCCCCCCCC	26.49	30576142
1181	Phosphorylation	RRRSSAASVGRADAP CCCCCCCCCCCCCCC	25.48	26074081

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
249	S	Phosphorylation	Kinase	CDK5	Q00535	PSP
279	S	Phosphorylation	Kinase	CDK5	Q00535	PSP
472	S	Phosphorylation	Kinase	AKT1	P31749	PSP
629	S	Phosphorylation	Kinase	AKT1	P31749	PSP
682	Y	Phosphorylation	Kinase	FYN	P06241	PSP
774	Y	Phosphorylation	Kinase	FYN	P06241	PSP

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of AGAP2_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of AGAP2_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
E41L1_HUMAN	EPB41L1	physical	11136977
P85A_HUMAN	PIK3R1	physical	11136977
MPRIP_HUMAN	MPRIP	physical	20368287
MPRIP_MOUSE	Mprip	physical	20368287
NR4A1_MOUSE	Nr4a1	physical	20368287
SIA1A_MOUSE	Siah1a	physical	20368287
GRIP1_HUMAN	GRIP1	physical	21847098
GRIA2_HUMAN	GRIA2	physical	21847098
CDC42_HUMAN	CDC42	physical	22453919
RAC1_HUMAN	RAC1	physical	22453919
RAC2_HUMAN	RAC2	physical	22453919
RHOA_HUMAN	RHOA	physical	22453919
RAC1_MOUSE	Rac1	physical	22453919
ARF1_HUMAN	ARF1	physical	22453919
AKT1_HUMAN	AKT1	physical	22450747

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
There are no disease associations of PTM sites.
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of AGAP2_HUMAN

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Quantitative phosphoproteomic analysis of T cell receptor signalingreveals system-wide modulation of protein-protein interactions."; Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K.,Rodionov V., Han D.K.; Sci. Signal. 2:RA46-RA46(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-635 AND SER-638, ANDMASS SPECTROMETRY.	19690332 [Abstract]