WEE1_DROME - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: WEE1_DROME
• UniProt AC: P54350
• Protein Name: Wee1-like protein kinase
• Gene Name: Wee1 {ECO:0000312|FlyBase:FBgn0011737}
• Organism: Drosophila melanogaster (Fruit fly).
• Sequence Length: 609
• Subcellular Localization: Nucleus.
• Protein Description: Acts as a negative regulator of entry into mitosis (G2 to M transition). [PubMed: 8573790]
• Protein Sequence: MAFRQSEHEMSVTSLDSSVELRSRSPSPQVFNPRKLRFADDDFDKDTPEGASPQHPLQQRPKLSSGEEQQLDSKIGKEGGDGDVSMSPPCQKVRALRLFSTPATPKTILQKSTTQCSNHLSAAAAAVNASRRSDDLFRLSERPRSLPLHNRKLPTQDTANVNPFTPDSLMAHNKKRCRTQFGRENLNLNVNAMQKYLLSDACDDDVTEEAGDSMREIHQQAPKRLALHDTNISRFKREFMQVNVIGVGEFGVVFQCVNRLDGCIYAIKKSKKPVAGSSFEKRALNEVWAHAVLGKHDNVVRYYSAWAEDDHMLIQNEFCDGGSLHARIQDHCLGEAELKIVLMHVIEGLRYIHSNDLVHMDLKPENIFSTMNPNAHKLVEVQPQQTKDDDGMDSVYEELRHSENLVTYKIGDLGHVTSVKEPYVEEGDCRYLPKEILHEDYSNLFKADIFSLGITLFEAAGGGPLPKNGPEWHNLRDGKVPILPSLSRDFNELIAQMMHPYPDKRPTSQSIFSHPILSAVDSKSKLQLGLELTVEKRKNEILMNKLREAKKQIKLLEQRVNLLAVTNNPDSLDGQRCLRSFTRRMRTPFSSHGKFDSISDRNKNVITNI

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
23	Phosphorylation	DSSVELRSRSPSPQV CCCEEHHHCCCCCCC	50.13	19429919
25	Phosphorylation	SVELRSRSPSPQVFN CEEHHHCCCCCCCCC	30.86	19429919
27	Phosphorylation	ELRSRSPSPQVFNPR EHHHCCCCCCCCCCC	29.25	19429919
47	Phosphorylation	DDDFDKDTPEGASPQ CCCCCCCCCCCCCCC	29.04	19429919
52	Phosphorylation	KDTPEGASPQHPLQQ CCCCCCCCCCCCCCC	35.63	19429919
87	Phosphorylation	GDGDVSMSPPCQKVR CCCCCCCCCHHHCEE	20.41	25749252
100	Phosphorylation	VRALRLFSTPATPKT EEEHHHHCCCCCCHH	37.77	19429919
101	Phosphorylation	RALRLFSTPATPKTI EEHHHHCCCCCCHHH	15.03	22817900
104	Phosphorylation	RLFSTPATPKTILQK HHHCCCCCCHHHHHC	26.90	19429919
133	Phosphorylation	AVNASRRSDDLFRLS HHHHHHCCCCHHHHC	34.07	22817900
145	Phosphorylation	RLSERPRSLPLHNRK HHCCCCCCCCCCCCC	36.70	29892262
165	Phosphorylation	TANVNPFTPDSLMAH CCCCCCCCHHHHHHH	27.69	22817900
168	Phosphorylation	VNPFTPDSLMAHNKK CCCCCHHHHHHHCHH	22.65	22817900
594	Acetylation	TPFSSHGKFDSISDR CCCCCCCCCCCCCCC	40.34	21791702

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of WEE1_DROME !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of WEE1_DROME !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of WEE1_DROME !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
PGPLE_DROME	PGRP-LE	physical	14605208
JMJD6_DROME	PSR	physical	14605208
JNK_DROME	bsk	physical	14605208
RUX_DROME	rux	genetic	12072468
ATR_DROME	mei-41	genetic	10790391
CDK1_DROME	cdc2	genetic	12072468
LOK_DROME	lok	genetic	15589158
LOK_DROME	lok	genetic	16139207
KI10A_DROME	Klp10A	physical	25284370
CDK1_DROME	cdc2	physical	8573790

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"Phosphoproteome analysis of Drosophila melanogaster embryos.";
Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
J. Proteome Res. 7:1675-1682(2008).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-23; SER-25; SER-27;THR-47; SER-52; THR-165 AND SER-168, AND MASS SPECTROMETRY.
PubMed: 18327897