UBP2_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: UBP2_MOUSE
• UniProt AC: O88623
• Protein Name: Ubiquitin carboxyl-terminal hydrolase 2
• Gene Name: Usp2
• Organism: Mus musculus (Mouse).
• Sequence Length: 613
• Subcellular Localization: Cytoplasm . Cytoplasm, perinuclear region . Localizes in the spermatid head in late-elongating spermatids in the thin area between the outer acrosomal membrane and the plasma membrane.; Isoform 2: Nucleus . Membrane ; Peripheral membrane protein . Cy
• Protein Description: Hydrolase that deubiquitinates polyubiquitinated target proteins such as MDM2, MDM4 and CCND1 (By similarity). Isoform 1 and isoform 2 possess both ubiquitin-specific peptidase and isopeptidase activities (By similarity). Deubiquitinates MDM2 without reversing MDM2-mediated p53/TP53 ubiquitination and thus indirectly promotes p53/TP53 degradation and limits p53 activity (By similarity). Has no deubiquitinase activity against p53/TP53 (By similarity). Prevents MDM2-mediated degradation of MDM4 (By similarity). Plays a role in the G1/S cell-cycle progression in normal and cancer cells (By similarity). Plays a role in the regulation of myogenic differentiation of embryonic muscle cells (By similarity). Regulates the circadian clock by modulating its intrinsic circadian rhythm and its capacity to respond to external cues. [PubMed: 23213472]
• Protein Sequence: MSQLSSTLKRYTESSRYTDAPYAKPGYGTYTPSSYGANLAASFLEKEKLGFKPVSPTSFLPRPRTYGPSSILDCDRGRPLLRSDIIGSSKRSESQTRGNERPSGSGLNGGSGFSYGVSSNSLSYLPMNARDQGVTLSQKKSNSQSDLARDFSSLRTSDGYRTSEGFRIDPGNLGRSPMLARTRKELCALQGLYQAASRSEYLTDYLENYGRKGSAPQVLTQAPPPSRVPEVLSPTYRPSGRYTLWEKSKGQASGPSRSSSPGRDTMNSKSAQGLAGLRNLGNTCFMNSILQCLSNTRELRDYCLQRLYMRDLGHTSSAHTALMEEFAKLIQTIWTSSPNDVVSPSEFKTQIQRYAPRFMGYNQQDAQEFLRFLLDGLHNEVNRVAARPKASPETLDHLPDEEKGRQMWRKYLEREDSRIGDLFVGQLKSSLTCTDCGYCSTVFDPFWDLSLPIAKRGYPEVTLMDCMRLFTKEDILDGDEKPTCCRCRARKRCIKKFSVQRFPKILVLHLKRFSESRIRTSKLTTFVNFPLRDLDLREFASENTNHAVYNLYAVSNHSGTTMGGHYTAYCRSPVTGEWHTFNDSSVTPMSSSQVRTSDAYLLFYELASPPSRM

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
143	Phosphorylation	LSQKKSNSQSDLARD HHCCCCCCHHHHHHH	37.85	23737553
145	Phosphorylation	QKKSNSQSDLARDFS CCCCCCHHHHHHHHH	34.12	23737553
161	Methylation	LRTSDGYRTSEGFRI CCCCCCCCCCCCEEC	36.99	18966987
167	Methylation	YRTSEGFRIDPGNLG CCCCCCEECCCCCCC	43.17	18966993
214	Phosphorylation	ENYGRKGSAPQVLTQ HHCCCCCCCCCHHCC	39.55	28542873
226	Phosphorylation	LTQAPPPSRVPEVLS HCCCCCCCCCCCCCC	52.24	22324799
233	Phosphorylation	SRVPEVLSPTYRPSG CCCCCCCCCCCCCCC	22.00	28542873
239	Phosphorylation	LSPTYRPSGRYTLWE CCCCCCCCCCEEEEE	27.23	25338131
253	Phosphorylation	EKSKGQASGPSRSSS ECCCCCCCCCCCCCC	43.26	23375375
258	Phosphorylation	QASGPSRSSSPGRDT CCCCCCCCCCCCCCC	39.23	23375375
259	Phosphorylation	ASGPSRSSSPGRDTM CCCCCCCCCCCCCCC	39.17	23737553
260	Phosphorylation	SGPSRSSSPGRDTMN CCCCCCCCCCCCCCC	32.40	23737553
268	Phosphorylation	PGRDTMNSKSAQGLA CCCCCCCCCCHHHHH	19.71	29899451
391	Phosphorylation	VAARPKASPETLDHL HHHCCCCCHHHHHCC	29.42	27742792
394	Phosphorylation	RPKASPETLDHLPDE CCCCCHHHHHCCCCH	40.65	27742792
498	Phosphorylation	KRCIKKFSVQRFPKI HHHHHHHHCCCCCCE	26.64	20139300
522	Ubiquitination	ESRIRTSKLTTFVNF HHHCCCCCCEEEECC	49.60	22790023
522 (in isoform 3)	Ubiquitination	-	49.60	22790023
528 (in isoform 3)	Ubiquitination	-	28.73	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of UBP2_MOUSE !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of UBP2_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of UBP2_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
NEDD4_HUMAN	NEDD4	physical	21478478
NEDD4_MOUSE	Nedd4	physical	21478478
CRY1_MOUSE	Cry1	physical	21966515
BMAL1_MOUSE	Arntl	physical	21966515
KC1E_MOUSE	Csnk1e	physical	21966515
UBP2_MOUSE	Usp2	physical	14686789
UBP2_HUMAN	USP2	physical	14686789
PER1_MOUSE	Per1	physical	23213472
PER2_MOUSE	Per2	physical	23213472
CRY1_MOUSE	Cry1	physical	23213472
CRY2_MOUSE	Cry2	physical	23213472
BMAL1_MOUSE	Arntl	physical	23213472

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.